Fibrinolytic and Esterolytic Activity of Human Plasma

1965 ◽  
Vol 13 (02) ◽  
pp. 484-491
Author(s):  
D. M Adamis ◽  
G. M Maniatis
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Total Activity ◽  
Satisfactory Method ◽  
Acetone Treatment ◽  
Before And After ◽  
Esterolytic Activity ◽  
Hydrolysis Of

SummaryThe fibrinolytic and esterolytic activity of the plasma before and after activation with Streptokinase is studied. The inhibitors of plasmin are neutralized by the acidification of the plasma or by the acetone treatment. The inhibitors of the “trypsin like enzymes” are neutralized only by acetone. The hydrolysis of the BAEE by the acetone treated and activated plasma seems to be a satisfactory method for the simultaneous determination of the total (plasmin-plasminogen) activity and the total activity of the (trypsin like enzymes) with arginine exopeptidase activity.

A METHOD FOR THE SIMULTANEOUS DETERMINATION OF 17-OXOSTEROIDS AND 17-HYDROXYCORTICOSTEROIDS IN HUMAN PLASMA

1964 ◽  
Vol 28 (3) ◽  
pp. 289-296 ◽  
Author(s):  
N. DESHPANDE ◽  
R. D. BULBROOK
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Silica Gel ◽  
Simultaneous Estimation ◽  
Simple Method ◽  
Conjugated Compounds ◽  
Hydrolysis Of

SUMMARY A simple method for the simultaneous estimation of 17-hydroxycorticosteroids and 17-oxosteroids in human plasma is described. It involves extraction of free corticosteroids, hydrolysis of glucuronosides with β-glucuronidase and solvolysis of sulphates, chromatography on silica gel and colorimetry using microcells. Recoveries of added steroids, using appropriate conjugated compounds were over 80%.

The Plasmin Inhibitors of Plasma

1964 ◽  
Vol 12 (01) ◽  
pp. 119-125 ◽  
Author(s):  
Y Shamash ◽  
A Rimon
Keyword(s):  
Human Plasma ◽  
New Method ◽  
Normal Amount ◽  
Caseinolytic Activity ◽  
Before And After ◽  
Plasmin Inhibitors

SummaryA new method for the assay of plasmin inhibitors in human plasma is described. The method consists of determination of the caseinolytic activity of a standard plasmin solution before and after incubation with the inhibitor, with lysine added to the mixture as a stabilizer of plasmin. Using this method, it was found that plasma contains enough inhibitors to inactivate 30 caseinolytic units of plasmin, or 10 times the normal amount of plasminogen in human plasma.

Simultaneous determination of propranolol or metoprolol in the presence of butyrophenones in human plasma by gas chromatography with mass spectrometry

1993 ◽  
Vol 82 (1) ◽  
pp. 87-90 ◽  
Author(s):  
Maria Pia Quaglio ◽  
Anna Maria Bellini ◽  
Luciano Minozzi ◽  
Guiseppe Frisina ◽  
Fabio Testoni
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Simultaneous Determination ◽  
Human Plasma
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