A METHOD FOR THE SIMULTANEOUS DETERMINATION OF 17-OXOSTEROIDS AND 17-HYDROXYCORTICOSTEROIDS IN HUMAN PLASMA
Keyword(s):
SUMMARY A simple method for the simultaneous estimation of 17-hydroxycorticosteroids and 17-oxosteroids in human plasma is described. It involves extraction of free corticosteroids, hydrolysis of glucuronosides with β-glucuronidase and solvolysis of sulphates, chromatography on silica gel and colorimetry using microcells. Recoveries of added steroids, using appropriate conjugated compounds were over 80%.
1984 ◽
Vol 308
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pp. 55-63
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2016 ◽
Vol 60
(8)
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pp. 4734-4742
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2009 ◽
Vol 877
(31)
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pp. 3953-3959
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2001 ◽
Vol 755
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pp. 85-89
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