scholarly journals The Lipolytic Activity of Antithrombin III

1977 ◽  
Author(s):  
M. Nakagawa ◽  
S. Okuda ◽  
M. Watada ◽  
H. Ijichi
Keyword(s):  
Myocardial Infarction ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Lipase Activity ◽  
Inhibitory Activity ◽  
Antithrombin Iii ◽  
Lipolytic Activity ◽  
Lipoprotein Lipase Activity ◽  
Coagulation Mechanism ◽  
The Relationship

Antithrombin III in the circulating blood is known to play an important role on the coagulation mechanism of the blood and is reported to be decreased in its amount on the cases of myocardial infarction and arteriosclerosis, in the meanwhile the arteriosclerosis has been considered to be one of thrombogenic factors. This research was designed to investigate the effects of antithrombin III on the relationship between fibrin formation and atherosclerosis.Antithrombin III was purified from human defibrinated plasma by Heparin Sepharose Affinity Chromatography. Using this antithrombin III as an enzyme, its lipolytic activity was assayed spectrophotometrically detecting the produced free fatty acid from various substrates, Intralipid, Triolein, and Human Chylomicron respectively. Lipoprotein lipase activity was detected in the purified antithrombin III. Intralipid was hydrolyzed to a greater extent than triolein or chylomicron and it is concluded that antithrombin III in the circulating blood has not only the inhibitory activity of thrombin but also has the lipolytic activity. From these observations it is considered that this lipolytic activity is beneficial for preventing the developement of atherosclerosis and also of thrombosis.

Effect of prolactin on lipoprotein lipase in crop sac and adipose tissue of pigeons

1975 ◽  
Vol 228 (5) ◽  
pp. 1542-1544 ◽  
Author(s):  
MM Garrison ◽  
RO Scow
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Lipoprotein Lipase ◽  
Adult Female ◽  
Lipase Activity ◽  
Milk Lipid ◽  
Lipoprotein Lipase Activity ◽  
Omental Adipose Tissue ◽  
Chylomicron Triglyceride

The effect of prolactin on lipoprotein lipase activity of crop sac, omental adipose tissue, and esophagus was studied in adult female pigeons. Prolactin injected for 4 days, 1 mg/day, increased lipoprotein lipase activity from 17 to 177 U/g in crop sac and from 68 to 118 U/g in adipose tissue, but had no effect on the activity in esophagus, 4 U/g. (10 = 1 mumol of chylomicron triglyceride hydrolyzed to free fatty acid and glycerol per hour.? Prolactin increased the weight of crop sac from 1.4 to 7.2 G. The effect of prolactin on lipoprotein lipase activity and weight of crop sac occurred mostly during the 3rd and 4th days of treatment, whereas the effect on the activity of adipose tissue occurred later, during the 4th day of treatment. Crop "milk" collected from pigeons injected with 2 mg of prolactin daily for 4 days contained a small amount of lipoprotein lipase activity, 12 U/g, is smaller than 10% of that found in crop sac. The finding of markedly increased lipoprotein lipase activity in crop sac of prolactin-treated pigeons suggests that blood triglyceride may be used by crop sac for the formation of crop milk lipid.

Kinetics Of Anticoagulant And Lipolytic Activity And Radiolabel After Intravenous Administration Of 35S-Heparin Fractions Of Different Molecular Weight

1981 ◽  
Author(s):  
C A M de Swart ◽  
J J Sixma ◽  
A Nijmeijer ◽  
E Holmer ◽  
L O Andersson ◽  
...  
Keyword(s):  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Antithrombin Iii ◽  
Lipolytic Activity ◽  
Convex Curve ◽  
Lipoprotein Lipase Activity ◽  
Hepatic Triglyceride ◽  
Triglyceride Lipase ◽  
Heparin Fractions ◽  
Hepatic Triglyceride Lipase

Heparin fractions were prepared from commercial pig mucosal by affinity chromatography on antithrombin III, 35S- radiolabelling and gelchromatography. Three fractions were obtained with moLwt.of < 6.000, 6 - 35.000 and > 35.000 daltons. They were administered intravenous as a bolus into human volunteers. The anticoagulant activity was measured with the APTT and Xa inactivation, using standard curves. The lipolytic activity was as^ed as hepatic triglyceride lipase activity, and as extra-hepatic lipoprotein lipase activity by hydrolysis of tritiated triolein. Radioactivity data were corrected for degraded heparin fragments uncomplexed to protein by subtracting radiolabel with a mol.wt. < 10.000 passing through a filter.Low mol.wt. fractions induced neither of both lipase activity and had no effect on the APTT. Anti Xa activity and radioactivity disappeared in parallel with slightly concave curves in semilogarthmic plots. Heparin of intermediate and high mol.wt. induced both lipolytic and both anticoagulant activities. The elimination of radioactivity, hepatic triglyceride lipase activity and anti-Xa activity occurred parallel according to a convex curve in semilogarthmic plots. The extra-hepatic lipoprotein lipase activity disappeared following a slightly concave curve.These data indicate that relatively large heparin molecules are required for lipolytic and APTT activity. Hepatic triglyceride lipase activity might be present complexed to heparin, comparable to the antithrombin III-heparin complex. The elimination of anti Xa activity and hepatic triglyceride lipase activity might be determined by the heparin part of the complexes.

scholarly journals Clearing-factor lipase in adipose tissue. A possible role of adenosine 3′,5′-(cyclic)-monophosphate in the regulation of its activity

1968 ◽  
Vol 109 (5) ◽  
pp. 841-849 ◽  
Author(s):  
D. R. Wing ◽  
D S Robinson
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Cyclic Amp ◽  
Lipase Activity ◽  
Glucose Concentration ◽  
Free Fatty Acid Concentration ◽  
Epididymal Fat ◽  
Fat Bodies

1. The rise in clearing-factor lipase activity that occurs when epididymal fat bodies from starved rats are incubated in appropriate media in vitro is inhibited in the presence of 6-N-2′-O-dibutyryl-3′,5′-(cyclic)-AMP (1mm). 2. Inhibition occurs at a concentration of glucose in the incubation medium of 1·3mg./ml. or less, but not at a glucose concentration of 2·4mg./ml., unless caffeine (1mm), an inhibitor of 3′,5′-(cyclic)-nucleotide phosphodiesterase, is also present. Caffeine (5mm) alone inhibits the rise in clearing-factor lipase activity at a glucose concentration of 2·4mg./ml. of medium. 3. The concentration of free fatty acids in the epididymal fat bodies normally falls during incubations in vitro as the rise in clearing-factor lipase activity occurs. In the presence of 1mm-6-N-2′-O-dibutyryl-3′,5′-(cyclic)-AMP, however, either the tissue free fatty acid concentration is increased or it does not fall to the same extent. The concentration of glucose in the incubation medium is important in determining the direction and extent of the changes in tissue free fatty acid concentration that occur in the presence of 6-N-2′-O-dibutyryl-3′,5′-(cyclic)-AMP. 4. Free fatty acid concentrations in epididymal fat bodies in vivo rise as the clearing-factor lipase activity of the tissue falls during starvation. 5. The possibility that the concentration of 3′,5′-(cyclic)-AMP in adipose tissue may regulate clearing-factor lipase activity, and that the regulation may occur through effects of the nucleotide on tissue free fatty acid concentrations, is discussed.

Influence of processing temperature and seasonal change in diet on lipase activity and lipolysis during the mechanical separation of bovine milk

1985 ◽  
Vol 52 (2) ◽  
pp. 255-266 ◽  
Author(s):  
Eric C. Needs ◽  
Malcolm Anderson ◽  
Stuart J. Payne ◽  
Elizabeth A. Ridout
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Seasonal Change ◽  
Lipase Activity ◽  
Bovine Milk ◽  
Skim Milk ◽  
Relative Activity ◽  
Heat Inactivation ◽  
Processing Temperature ◽  
Original Activity

SUMMARYThe effect of separating conditions on lipase activity and free fatty acid levels in preheated milk, cream and skim milk was measured on nine occasions during a 12-week period covering the seasonal change from winter feeding to summertime grazing. This change consisted of four periods each representing a different type of forage intake, namely: silage, kale, daytime grazing and 24 h grazing. Milk was separated at 30, 40, 50 and 60°C with preheating times of 10, 25 and 55 s. Results were expressed both as absolute values and in terms of changes relative to the original unheated milk. Lipase activity and free fatty acid concentration were significantly reduced as separation temperature increased but were not influenced by holding time. The loss of activity in cream was progressive so that at 60°C only 40% of the original activity remained. Up to 50°C little change occurred in preheated milk or skim milk activity, while at 60°C 83 and 76% respectively of the original activity remained. The amount of activity calculated to be associated with the fat fraction of the cream also decreased with temperature. Activity varied significantly with date; maximum values were observed during the first 3 weeks of summertime grazing. Relative activity values indicated that the susceptibility of milk lipase to heat inactivation also varied with date. Lipolysis was also significantly affected by date. Cream free fatty acid levels were lower during the period of daytime grazing and were significantly higher than those in preheated milk. The correlation between lipase activity and free fatty acid levels was generally poor, accounting for between 0 and 34% of the variance. Possible reasons for the effect of separating temperature on lipolysis in cream are discussed.

Influence of hypothyroidism on the lipolytic activity of norepinephrine in the newborn lamb

1986 ◽  
Vol 108 (3) ◽  
pp. 451-454 ◽  
Author(s):  
C. Wrutniak ◽  
G. Cabello
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Thyroid Hormones ◽  
Lipolytic Activity ◽  
Plasma Free Fatty Acid ◽  
Newborn Lamb ◽  
Group A ◽  
Group B ◽  
Infusion Of Norepinephrine ◽  
Medium Group

ABSTRACT The effects of hypothyroidism on the lipolytic activity of norepinephrine were assessed in the newborn lamb. Lambs were separated into three groups: group A were controls; groups B and C were made hypothyroid by administration of benzylthiouracil from birth until 11 days of age. In control lambs, plasma free fatty acid concentrations, used as an index of lipolytic activity, increased significantly (plus 0·45 mmol/l) during the infusion of norepinephrine, whereas they did not change in hypothyroid lambs (group B). Adding thyroxine and tri-iodothyronine to the infusion medium (group C) immediately restored the free fatty acid response to norepinephrine in hypothyroid lambs (plus 0·41 mmol/l). These results suggest that thyroid hormones could modulate the lipolytic activity of catecholamines in the newborn lamb without a latent period. J. Endocr. (1986) 108, 451–454

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