scholarly journals Tissue engineering approaches for the construction of a completely autologous tendon substitute

2008 ◽  
Vol 41 (01) ◽  
pp. 38-46
Author(s):  
Bassetto Franco ◽  
Vindigni Vincenzo ◽  
Dalla Vedova Alessandro ◽  
Carolin Tonello ◽  
Giovanni Abatangelo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
In Vitro Regeneration ◽  
Mechanical Stimuli ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering ◽  
Mammalian Tissues ◽  
Fundamental Understanding ◽  
In Vitro Preservation

ABSTRACTTissue engineering is a multidisciplinary field that involves the application of the principles and methods of engineering and life sciences towards i) the fundamental understanding of structure-function relationships in normal and pathological mammalian tissues and ii) the development of biological substitutes that restore, maintain or improve tissue function. The goal of tissue engineering is to surpass the limitations of conventional treatments based on organ transplantation and biomaterial implantation. The field of tendon tissue engineering is relatively unexplored due to the difficulty in in vitro preservation of tenocyte phenotype. Only recently has mechanobiology allowed us to gain a better understanding of the fundamental role of in vitro mechanical stimuli in maintaining the phenotype of tendinous tissue. This review analyzes the techniques used so far for in vitro regeneration of tendinous tissue.

A Poly(lactic-co-glycolic acid) Knitted Scaffold for Tendon Tissue Engineering: An In Vitro and In Vivo Study

2010 ◽  
Vol 21 (13) ◽  
pp. 1737-1760 ◽  
Author(s):  
Cédryck Vaquette ◽  
Saïd Slimani ◽  
Cyril J. F. Kahn ◽  
Nguyen Tran ◽  
Rachid Rahouadj ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Glycolic Acid ◽  
In Vivo Study ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering

scholarly journals Anisotropic cytocompatible electrospun scaffold for tendon tissue engineering elicits limited inflammatory response in vitro

2018 ◽  
Vol 33 (1) ◽  
pp. 127-139 ◽  
Author(s):  
Andrea Fotticchia ◽  
David Musson ◽  
Cristina Lenardi ◽  
Emrah Demirci ◽  
Yang Liu
Keyword(s):  
Tissue Engineering ◽  
Inflammatory Response ◽  
Social Impact ◽  
Polymer Processing ◽  
High Recurrence Rate ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering ◽  
Tendon Tears

Tendon tears are a relevant concern for today’s national health systems because of their social impact and high recurrence rate. The current gold standard for fixing tendon tears is surgical repair; however, this strategy is not able to fully re-establish tendon integrity and functionality. Tissue engineering approaches aim at promoting tissue regeneration by delivering the opportune signals to the injured site combining biomaterials, cells and biochemical cues. Electrospinning is currently one of the most versatile polymer processing techniques that allows manufacturing of nano- and micro-fibres substrates. Such fibrous morphology is deemed to be an ideal substrate to convey topographical cues to cells. Here we evaluated the potential of polycaprolactone processed by means of electrospinning technology for tendon tissue engineering. Fibrous free-of-defects substrate with random and aligned fibres were successfully fabricated. Rat tenocytes were used to assess the cytocompatibility of the substrates for application as tendon tissue engineered devices. Tenocytes were able to proliferate and adapt to the substrates topography acquiring an elongated morphology, which is the precondition for oriented collagen deposition, when seeded on aligned fibres. Real time Polymerase Chain Reaction (Rt-PCR) also revealed the overall maintenance of tenocyte phenotype over 7 days culture. To verify suitability for in vivo implantation, the level of inflammatory cytokine genes expressed by THP-1 cells cultured in presence of electrospun polycaprolactone substrates was evaluated. Inflammatory response was limited. The novel preliminary in vitro work presented herein showing tenocytes compatibility and limited inflammatory cytokines synthesis suggests that electrospun polycaprolactone may be taken into consideration as substrate for tendon healing applications.

scholarly journals A bioreactor system for in vitro tendon differentiation and tendon tissue engineering

2015 ◽  
Vol 33 (6) ◽  
pp. 911-918 ◽  
Author(s):  
Daniel W. Youngstrom ◽  
Ibtesam Rajpar ◽  
David L. Kaplan ◽  
Jennifer G. Barrett
Keyword(s):  
Tissue Engineering ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering ◽  
Bioreactor System

scholarly journals Tension Stimulation of Tenocytes in Aligned Hyaluronic Acid/Platelet-Rich Plasma-Polycaprolactone Core-Sheath Nanofiber Membrane Scaffold for Tendon Tissue Engineering

2021 ◽  
Vol 22 (20) ◽  
pp. 11215
Author(s):  
Chih-Hao Chen ◽  
Dai-Ling Li ◽  
Andy Deng-Chi Chuang ◽  
Banendu Sunder Dash ◽  
Jyh-Ping Chen
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Hyaluronic Acid ◽  
Cellular Response ◽  
Platelet Rich Plasma ◽  
Nanofiber Membrane ◽  
Tendon Tissue ◽  
Fiber Alignment ◽  
Tendon Tissue Engineering

To recreate the in vivo niche for tendon tissue engineering in vitro, the characteristics of tendon tissue underlines the use of biochemical and biophysical cues during tenocyte culture. Herein, we prepare core-sheath nanofibers with polycaprolactone (PCL) sheath for mechanical support and hyaluronic acid (HA)/platelet-rich plasma (PRP) core for growth factor delivery. Three types of core-sheath nanofiber membrane scaffolds (CSNMS), consisting of random HA-PCL nanofibers (Random), random HA/PRP-PCL nanofibers (Random+) or aligned HA/PRP-PCL (Align+) nanofibers, were used to study response of rabbit tenocytes to biochemical (PRP) and biophysical (fiber alignment) stimulation. The core-sheath structures as well as other pertinent properties of CSNMS have been characterized, with Align+ showing the best mechanical properties. The unidirectional growth of tenocytes, as induced by aligned fiber topography, was confirmed from cell morphology and cytoskeleton expression. The combined effects of PRP and fiber alignment in Align+ CSNMS lead to enhanced cell proliferation rates, as well as upregulated gene expression and marker protein synthesis. Another biophysical cue on tenocytes was introduced by dynamic culture of tenocyte-seeded Align+ in a bioreactor with cyclic tension stimulation. Augmented by this biophysical beacon from mechanical loading, dynamic cell culture could shorten the time for tendon maturation in vitro, with improved cell proliferation rates and tenogenic phenotype maintenance, compared to static culture. Therefore, we successfully demonstrate how combined use of biochemical/topographical cues as well as mechanical stimulation could ameliorate cellular response of tenocytes in CSNMS, which can provide a functional in vitro environmental niche for tendon tissue engineering.

The Role of Bioreactors in Ligament and Tendon Tissue Engineering

2016 ◽  
Vol 11 (1) ◽  
pp. 35-40 ◽  
Author(s):  
James Mace ◽  
Andy Wheelton ◽  
Wasim S. Khan ◽  
Sanj Anand
Keyword(s):  
Tissue Engineering ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering

scholarly journals The Role of Scaffolds in Tendon Tissue Engineering

2020 ◽  
Vol 11 (4) ◽  
pp. 78
Author(s):  
Angelo V. Vasiliadis ◽  
Konstantinos Katakalos
Keyword(s):  
Tissue Engineering ◽  
Tendon Repair ◽  
Treatment Strategies ◽  
Tendon Injury ◽  
Functional Restoration ◽  
Tendon Tissue ◽  
Age Related ◽  
Tendon Tissue Engineering ◽  
Promising Solution

Tendons are unique forms of connective tissue aiming to transmit the mechanical force of muscle contraction to the bones. Tendon injury may be due to direct trauma or might be secondary to overuse injury and age-related degeneration, leading to inflammation, weakening and subsequent rupture. Current traditional treatment strategies focus on pain relief, reduction of the inflammation and functional restoration. Tendon repair surgery can be performed in people with tendon injuries to restore the tendon’s function, with re-rupture being the main potential complication. Novel therapeutic approaches that address the underlying pathology of the disease is warranted. Scaffolds represent a promising solution to the challenges associated with tendon tissue engineering. The ideal scaffold for tendon tissue engineering needs to exhibit physiologically relevant mechanical properties and to facilitate functional graft integration by promoting the regeneration of the native tissue.

scholarly journals Platelet-Rich Fibrin Scaffolds for Cartilage and Tendon Regenerative Medicine: From Bench to Bedside

2019 ◽  
Vol 20 (7) ◽  
pp. 1701 ◽  
Author(s):  
Silvia Barbon ◽  
Elena Stocco ◽  
Veronica Macchi ◽  
Martina Contran ◽  
Francesca Grandi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Regenerative Medicine ◽  
Related Factors ◽  
Surgical Interventions ◽  
Platelet Rich Fibrin ◽  
Tendon Tissue Engineering ◽  
Ready Availability

Nowadays, research in Tissue Engineering and Regenerative Medicine is focusing on the identification of instructive scaffolds to address the requirements of both clinicians and patients to achieve prompt and adequate healing in case of injury. Among biomaterials, hemocomponents, and in particular Platelet-rich Fibrin matrices, have aroused widespread interest, acting as delivery platforms for growth factors, cytokines and immune/stem-like cells for immunomodulation; their autologous origin and ready availability are also noteworthy aspects, as safety- and cost-related factors and practical aspects make it possible to shorten surgical interventions. In fact, several authors have focused on the use of Platelet-rich Fibrin in cartilage and tendon tissue engineering, reporting an increasing number of in vitro, pre-clinical and clinical studies. This narrative review attempts to compare the relevant advances in the field, with particular reference being made to the regenerative role of platelet-derived growth factors, as well as the main pre-clinical and clinical research on Platelet-rich Fibrin in chondrogenesis and tenogenesis, thereby providing a basis for critical revision of the topic.

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