scholarly journals Tension Stimulation of Tenocytes in Aligned Hyaluronic Acid/Platelet-Rich Plasma-Polycaprolactone Core-Sheath Nanofiber Membrane Scaffold for Tendon Tissue Engineering

2021 ◽  
Vol 22 (20) ◽  
pp. 11215
Author(s):  
Chih-Hao Chen ◽  
Dai-Ling Li ◽  
Andy Deng-Chi Chuang ◽  
Banendu Sunder Dash ◽  
Jyh-Ping Chen
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Hyaluronic Acid ◽  
Cellular Response ◽  
Platelet Rich Plasma ◽  
Nanofiber Membrane ◽  
Tendon Tissue ◽  
Fiber Alignment ◽  
Tendon Tissue Engineering

To recreate the in vivo niche for tendon tissue engineering in vitro, the characteristics of tendon tissue underlines the use of biochemical and biophysical cues during tenocyte culture. Herein, we prepare core-sheath nanofibers with polycaprolactone (PCL) sheath for mechanical support and hyaluronic acid (HA)/platelet-rich plasma (PRP) core for growth factor delivery. Three types of core-sheath nanofiber membrane scaffolds (CSNMS), consisting of random HA-PCL nanofibers (Random), random HA/PRP-PCL nanofibers (Random+) or aligned HA/PRP-PCL (Align+) nanofibers, were used to study response of rabbit tenocytes to biochemical (PRP) and biophysical (fiber alignment) stimulation. The core-sheath structures as well as other pertinent properties of CSNMS have been characterized, with Align+ showing the best mechanical properties. The unidirectional growth of tenocytes, as induced by aligned fiber topography, was confirmed from cell morphology and cytoskeleton expression. The combined effects of PRP and fiber alignment in Align+ CSNMS lead to enhanced cell proliferation rates, as well as upregulated gene expression and marker protein synthesis. Another biophysical cue on tenocytes was introduced by dynamic culture of tenocyte-seeded Align+ in a bioreactor with cyclic tension stimulation. Augmented by this biophysical beacon from mechanical loading, dynamic cell culture could shorten the time for tendon maturation in vitro, with improved cell proliferation rates and tenogenic phenotype maintenance, compared to static culture. Therefore, we successfully demonstrate how combined use of biochemical/topographical cues as well as mechanical stimulation could ameliorate cellular response of tenocytes in CSNMS, which can provide a functional in vitro environmental niche for tendon tissue engineering.

A Poly(lactic-co-glycolic acid) Knitted Scaffold for Tendon Tissue Engineering: An In Vitro and In Vivo Study

2010 ◽  
Vol 21 (13) ◽  
pp. 1737-1760 ◽  
Author(s):  
Cédryck Vaquette ◽  
Saïd Slimani ◽  
Cyril J. F. Kahn ◽  
Nguyen Tran ◽  
Rachid Rahouadj ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Glycolic Acid ◽  
In Vivo Study ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering

scholarly journals Anisotropic cytocompatible electrospun scaffold for tendon tissue engineering elicits limited inflammatory response in vitro

2018 ◽  
Vol 33 (1) ◽  
pp. 127-139 ◽  
Author(s):  
Andrea Fotticchia ◽  
David Musson ◽  
Cristina Lenardi ◽  
Emrah Demirci ◽  
Yang Liu
Keyword(s):  
Tissue Engineering ◽  
Inflammatory Response ◽  
Social Impact ◽  
Polymer Processing ◽  
High Recurrence Rate ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering ◽  
Tendon Tears

Tendon tears are a relevant concern for today’s national health systems because of their social impact and high recurrence rate. The current gold standard for fixing tendon tears is surgical repair; however, this strategy is not able to fully re-establish tendon integrity and functionality. Tissue engineering approaches aim at promoting tissue regeneration by delivering the opportune signals to the injured site combining biomaterials, cells and biochemical cues. Electrospinning is currently one of the most versatile polymer processing techniques that allows manufacturing of nano- and micro-fibres substrates. Such fibrous morphology is deemed to be an ideal substrate to convey topographical cues to cells. Here we evaluated the potential of polycaprolactone processed by means of electrospinning technology for tendon tissue engineering. Fibrous free-of-defects substrate with random and aligned fibres were successfully fabricated. Rat tenocytes were used to assess the cytocompatibility of the substrates for application as tendon tissue engineered devices. Tenocytes were able to proliferate and adapt to the substrates topography acquiring an elongated morphology, which is the precondition for oriented collagen deposition, when seeded on aligned fibres. Real time Polymerase Chain Reaction (Rt-PCR) also revealed the overall maintenance of tenocyte phenotype over 7 days culture. To verify suitability for in vivo implantation, the level of inflammatory cytokine genes expressed by THP-1 cells cultured in presence of electrospun polycaprolactone substrates was evaluated. Inflammatory response was limited. The novel preliminary in vitro work presented herein showing tenocytes compatibility and limited inflammatory cytokines synthesis suggests that electrospun polycaprolactone may be taken into consideration as substrate for tendon healing applications.

scholarly journals A bioreactor system for in vitro tendon differentiation and tendon tissue engineering

2015 ◽  
Vol 33 (6) ◽  
pp. 911-918 ◽  
Author(s):  
Daniel W. Youngstrom ◽  
Ibtesam Rajpar ◽  
David L. Kaplan ◽  
Jennifer G. Barrett
Keyword(s):  
Tissue Engineering ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering ◽  
Bioreactor System

scholarly journals Tissue engineering approaches for the construction of a completely autologous tendon substitute

2008 ◽  
Vol 41 (01) ◽  
pp. 38-46
Author(s):  
Bassetto Franco ◽  
Vindigni Vincenzo ◽  
Dalla Vedova Alessandro ◽  
Carolin Tonello ◽  
Giovanni Abatangelo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
In Vitro Regeneration ◽  
Mechanical Stimuli ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering ◽  
Mammalian Tissues ◽  
Fundamental Understanding ◽  
In Vitro Preservation

ABSTRACTTissue engineering is a multidisciplinary field that involves the application of the principles and methods of engineering and life sciences towards i) the fundamental understanding of structure-function relationships in normal and pathological mammalian tissues and ii) the development of biological substitutes that restore, maintain or improve tissue function. The goal of tissue engineering is to surpass the limitations of conventional treatments based on organ transplantation and biomaterial implantation. The field of tendon tissue engineering is relatively unexplored due to the difficulty in in vitro preservation of tenocyte phenotype. Only recently has mechanobiology allowed us to gain a better understanding of the fundamental role of in vitro mechanical stimuli in maintaining the phenotype of tendinous tissue. This review analyzes the techniques used so far for in vitro regeneration of tendinous tissue.

Core–Shell Poly(l-lactic acid)-Hyaluronic Acid Nanofibers for Cell Culture and Pelvic Ligament Tissue Engineering

2021 ◽  
Vol 17 (3) ◽  
pp. 399-406
Author(s):  
Di Zhang ◽  
Xiaobo Huang ◽  
Huaiming Wang ◽  
Yingqi Wei ◽  
Zifeng Yang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Hyaluronic Acid ◽  
Cellular Response ◽  
Pelvic Organ ◽  
Core Shell ◽  
Mouse Bone Marrow ◽  
Gene Markers ◽  
Ligament Tissue Engineering

Pelvic organ prolapse (POP) has become one of the most common serious diseases affecting parous women. Weakening of pelvic ligaments plays an essential role in the pathophysiology of POP. Currently, synthetic materials are widely applied for pelvic reconstructive surgery. However, synthetic nondegradable meshes for POP therapy cannot meet the clinical requirements due to its poor biocompatibility. Herein, we fabricated electrospun core–shell nanofibers of poly(l-lactic acid)-hyaluronic acid (PLLA/HA). After that, we combined them with mouse bone marrow-derived mesenchymal stem cells (mBMSCs) to assess the cellular response and pelvic ligament tissue engineering in vitro. The cellular responses on the composite nanofibers showed that the core–shell structure nanofibers displayed with excellent biocompatibility and enhanced cellular activity without cytotoxicity. Moreover, compared with PLLA nanofibers seeded with mBMSCs, PLLA/HA nanofibers exhibited more cellular function, as revealed by the quantitative real-time polymerase chain reaction (RT-qPCR) for pelvic ligament-related gene markers including Col1a1, Col1a3 and Tnc. These features suggested that this novel core–shell nanofiber is promising in stem cell-based tissue engineering for pelvic reconstruction.

scholarly journals Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age

2021 ◽  
Vol 22 (4) ◽  
pp. 1725
Author(s):  
Diego Delgado ◽  
Ane Miren Bilbao ◽  
Maider Beitia ◽  
Ane Garate ◽  
Pello Sánchez ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cellular Response ◽  
Platelet Rich Plasma ◽  
Biological Response ◽  
In Vitro Models ◽  
Molecular Composition ◽  
Cellular Models ◽  
The Central Nervous System

Platelet-rich plasma (PRP) is a biologic therapy that promotes healing responses across multiple medical fields, including the central nervous system (CNS). The efficacy of this therapy depends on several factors such as the donor’s health status and age. This work aims to prove the effect of PRP on cellular models of the CNS, considering the differences between PRP from young and elderly donors. Two different PRP pools were prepared from donors 65–85 and 20–25 years old. The cellular and molecular composition of both PRPs were analyzed. Subsequently, the cellular response was evaluated in CNS in vitro models, studying proliferation, neurogenesis, synaptogenesis, and inflammation. While no differences in the cellular composition of PRPs were found, the molecular composition of the Young PRP showed lower levels of inflammatory molecules such as CCL-11, as well as the presence of other factors not found in Aged PRP (GDF-11). Although both PRPs had effects in terms of reducing neural progenitor cell apoptosis, stabilizing neuronal synapses, and decreasing inflammation in the microglia, the effect of the Young PRP was more pronounced. In conclusion, the molecular composition of the PRP, conditioned by the age of the donors, affects the magnitude of the biological response.

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