Investigation of the marine microorganism Cladosporium halotolerans for the isolation and identification of bioactive metabolites with potential anti-aging activity

2019 ◽  
Author(s):  
IA Nikolaou ◽  
N Tsafantakis ◽  
P Vlachou ◽  
E Baira ◽  
A Sklirou ◽  
...  
Keyword(s):  
Bioactive Metabolites ◽  
Marine Microorganism ◽  
Isolation And Identification

scholarly journals CYTOTOXIC METABOLITES OF ALTERNARIA ALTERNATA, AN ENDOPHYTE OF THE MEDICINAL PLANT BIDENS BIPINNATA

2020 ◽  
pp. 42-48
Author(s):  
RANDA ABDOU ◽  
MOHAMED DAWOUD
Keyword(s):  
Medicinal Plant ◽  
Cell Lines ◽  
Cancer Cell ◽  
Alternaria Alternata ◽  
Cytostatic Activity ◽  
Bioactive Metabolites ◽  
Isolation And Identification ◽  
Antitumor Effects ◽  
Chromatographic Fractionation ◽  
Promising Source

Objective: Endophytes are widely spread in the plant kingdom and represent a very promising source of biologically active natural products. The medicinal plant Bidens bipinnata Lin. (Asteraceae) which is known for its anti-inflammatory, antifungal and antitumor effects has been chosen for the investigation of its endophyte to search for bioactive metabolites. Methods: An endophytic Alternaria alternata species was isolated from the leaves of the plant B. bipinnata Lin. To investigate the metabolic profile of this endophytic fungus it was cultivated in several culture media as static and shaken culture. The antimicrobial and cytotoxic activities of the ethyl acetate extracts of the fungus were examined. Extracts exhibiting highest antimicrobial activities in agar diffusion assay and cytotoxicity against HeLa cancer cell line were subjected to activity-guided chromatographic fractionation for the identification of bioactive metabolites. A cytotoxic assay was performed on the isolated compounds against HeLa cancer cell lines as well as cytostatic activity tests against HUVEC and K-562 cell lines. Results: Chromatographic fractionation resulted in the isolation and identification of alternariol and tentoxin from the extract of the fungus cultivated in medium M5 while sterigmatocystin was isolated in addition to alternariol and tentoxin from the extract of the fungus grown in medium M25. Both alternariol and sterigmatocystin proved to be of moderate cytotoxicity and weak cytostatic activity with alternariol showing higher cytotoxic activity than sterigmatocystin. Highest cytotoxicity against HeLa cell lines was observed for tentoxin with a CC50 of 22.5 µg/ml. Conclusion: This study presents the isolation and identification of the bioactive metabolites alternariol, sterigmatocystin and tentoxin from the endophyte A. alternata in addition to the antifungal activity of the strain extract as well as the cytotoxic and cytostatic activities of the isolated metabolites against HeLa, HUVEC and K-562 cell lines, respectively.

Research Progress on Bioactive Metabolites from Marine Microorganism and Their Structures

2010 ◽  
Vol 8 (4) ◽  
pp. 309-320 ◽  
Author(s):  
Xing WANG ◽  
Wen-Hui WU ◽  
Zhi-Hua CHEN ◽  
Jie ZHANG ◽  
Bin BAO
Keyword(s):  
Research Progress ◽  
Bioactive Metabolites ◽  
Marine Microorganism

Detection of pharmacologically active natural products using ecology. Selected examples from Indopacific marine invertebrates and sponge-derived fungi

2003 ◽  
Vol 75 (2-3) ◽  
pp. 343-352 ◽  
Author(s):  
P. Proksch ◽  
R. Ebel ◽  
R. A. Edrada ◽  
P. Schupp ◽  
W. H. Lin ◽  
...  
Keyword(s):  
Natural Products ◽  
Marine Invertebrates ◽  
Marine Sponges ◽  
Bioactive Metabolites ◽  
Marine Biodiversity ◽  
Marine Microorganisms ◽  
Bioactive Constituents ◽  
Isolation And Identification ◽  
Bioactive Natural Products ◽  
Pharmacologically Active

This review article presents our group's recent research findings with regard to bioactive natural products from marine sponges and tunicates, as well as from sponge derived fungi. The organisms discussed originate in the Indopacific region, which has an exceptionally rich marine biodiversity. Major topics that are covered in our review include the chemical ecology of sponges, focusing on defense against fishes, as well as the isolation and identification of new bioactive constituents from sponges and tunicates. Sponge derived fungi are introduced as an emerging source for new bioactive metabolites, reflecting the currently growing interest in natural products from marine microorganisms.

scholarly journals Taxonomy and Characterization of Bioactive Metabolites from a New Marine Microorganism Strain MLA-21

2013 ◽  
Vol 25 (1) ◽  
pp. 220-224 ◽  
Author(s):  
Yingying Xing ◽  
Yihua Ma ◽  
Yuanyuan Lu ◽  
Tao Xi
Keyword(s):  
Bioactive Metabolites ◽  
Marine Microorganism

scholarly journals Isolation and Identification of DHA-Rich Marine Microorganism

2014 ◽  
Vol 47 (1) ◽  
pp. 31-38
Author(s):  
U-Cheol Jeong ◽  
Byeong-Dae Choi ◽  
Jong-Duck Choi ◽  
Seok-Joong Kang
Keyword(s):  
Marine Microorganism ◽  
Isolation And Identification

scholarly journals Isolation and Characterization of Novel Marine-Derived Actinomycete Taxa Rich in Bioactive Metabolites

2004 ◽  
Vol 70 (12) ◽  
pp. 7520-7529 ◽  
Author(s):  
Nathan A. Magarvey ◽  
Jessica M. Keller ◽  
Valerie Bernan ◽  
Martin Dworkin ◽  
David H. Sherman
Keyword(s):  
16S Rrna ◽  
Multidrug Resistant ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Bioactive Metabolites ◽  
Rrna Gene ◽  
New Genera ◽  
Isolation And Identification ◽  
Fermentation Products ◽  
Isolation And Characterization

ABSTRACT A unique selective enrichment procedure has resulted in the isolation and identification of two new genera of marine-derived actinobacteria. Approximately 90% of the microorganisms cultured by using the presented method were from the prospective new genera, a result indicative of its high selectivity. In this study, 102 actinomycetes were isolated from subtidal marine sediments collected from the Bismarck Sea and the Solomon Sea off the coast of Papua New Guinea. A combination of physiological parameters, chemotaxonomic characteristics, distinguishing 16S rRNA gene sequences, and phylogenetic analysis based on 16S rRNA genes provided strong evidence for the two new genera (represented by strains of the PNG1 clade and strain UMM518) within the family Micromonosporaceae. Biological activity testing of fermentation products from the new marine-derived actinomycetes revealed that several had activities against multidrug-resistant gram-positive pathogens, malignant cells, and vaccinia virus replication.

Targeted isolation of induced and bioactive metabolites from fungal co-cultures

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
N Bohni ◽  
O Schumpp ◽  
S Schnee ◽  
S Bertrand ◽  
K Gindro ◽  
...  
Keyword(s):  
Bioactive Metabolites

Investigation into the bioactive metabolites of deep sea fungi

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
CL Bromley ◽  
RM Young ◽  
S Jackson ◽  
T Sutton ◽  
A Dobson ◽  
...  
Keyword(s):  
Deep Sea ◽  
Bioactive Metabolites
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