scholarly journals Concanavalin A Enhanced Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells

2020 ◽  
Vol 14 (01) ◽  
pp. 123-127
Author(s):  
Ketut Suardita ◽  
Ira Arundina ◽  
Udijanto Tedjosasongko ◽  
Anita Yuliati ◽  
Harry Huiz Peeters ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Concanavalin A ◽  
Dental Pulp ◽  
In Vitro Study ◽  
Analysis Data ◽  
Dental Pulp Stem Cells ◽  
P Value ◽  
Animal Cells

Abstract Objective Dental pulp stem cells (DPSCs) can be used as a component in the formation of regenerative dentine during direct pulp capping therapy. Concanavalin A (ConA) is a type of lectin with a molecular weight of 26 kDa derived from the Canavalia ensiformis plant. Lectins possess strong proliferation and differentiation abilities in various animal cells including lymphocytes, osteoblasts, and chondrocytes. The aim of study was to determine the effect of ConA on the proliferation and osteogenic differentiation of DPSCs in vitro. Materials and Methods In this in vitro study, DPSCs were isolated from third molars before ConA induction was performed at concentrations of 5 and 10 μg/mL. The proliferation assay was determined by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Osteogenic differentiation was determined by means of mineralization. Statistical Analysis Data were analyzed using analysis of variance and a Student’s t-test. The p-value was set at 0.05. Results The addition of 5 and 10 µg/mL of ConA to DPSCs can significantly increase the proliferation and osteogenic differentiation of DPSCs (p ≤0.05). Conclusion ConA can increase the proliferation and osteogenic differentiation of DPSCs.

An In Vitro Study of the Effects of Crocin on the Modulation of DSPP, VEGF-A, HLA-G5, STAT3 and CD200 Expression in Human Dental Pulp Stem Cells

2021 ◽  
Author(s):  
Mansoore Saharkhiz ◽  
Fariba Emadian Razavi ◽  
Seyed Mohammad Riahi ◽  
Malaksima Ayadilord ◽  
Zeinab Rostami ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
In Vitro Study ◽  
Dental Pulp Stem Cells ◽  
Vitro Study ◽  
Human Dental Pulp

scholarly journals Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem Cells In Vitro

2015 ◽  
Vol 21 (3-4) ◽  
pp. 729-739 ◽  
Author(s):  
Jonas Jensen ◽  
David Christian Evar Kraft ◽  
Helle Lysdahl ◽  
Casper Bindzus Foldager ◽  
Muwan Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Effect of Vitamins D and E on the Proliferation, Viability, and Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Lina M. Escobar ◽  
Zita Bendahan ◽  
Andrea Bayona ◽  
Jaime E. Castellanos ◽  
María-Clara González
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Dental Pulp ◽  
Morphological Changes ◽  
In Vitro Study ◽  
Osteoblastic Differentiation ◽  
Dental Pulp Stem Cells ◽  
Alizarin Red ◽  
Human Dental Pulp

Introduction. The aim of the present study was to determine the effects of vitamins D and E on the proliferation, morphology, and differentiation of human dental pulp stem cells (hDPSCs). Methods. In this in vitro experimental study, hDPSCs were isolated, characterized, and treated with vitamins D and E, individually and in combination, utilizing different doses and treatment periods. Changes in morphology and cell proliferation were evaluated using light microscopy and the resazurin assay, respectively. Osteoblast differentiation was evaluated with alizarin red S staining and expression of RUNX2, Osterix, and Osteocalcin genes using real-time RT-PCR. Results. Compared with untreated cells, the number of cells significantly reduced following treatment with vitamin D (49%), vitamin E (35%), and vitamins D + E (61%) after 144 h. Compared with cell cultures treated with individual vitamins, cells treated with vitamins D + E demonstrated decreased cell confluence, with more extensive and flatter cytoplasm that initiated the formation of a significantly large number of calcified nodules after 7 days of treatment. After 14 days, treatment with vitamins D, E, and D + E increased the transcription of RUNX2, Osterix, and Osteocalcin genes. Conclusions. Vitamins D and E induced osteoblastic differentiation of hDPSCs, as evidenced by the decrease in cell proliferation, morphological changes, and the formation of calcified nodules, increasing the expression of differentiation genes. Concurrent treatment with vitamins D + E induces a synergistic effect in differentiation toward an osteoblastic lineage.

scholarly journals Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Casiano Del Angel-Mosqueda ◽  
Yolanda Gutiérrez-Puente ◽  
Ada Pricila López-Lozano ◽  
Ricardo Emmanuel Romero-Zavaleta ◽  
Andrés Mendiola-Jiménez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Epidermal Growth

Association of Dental Pulp Stem Cells and Ricinus Bone Compound in a Model of Bone Defect

2020 ◽  
Vol 3 (3) ◽  
pp. 267-278
Author(s):  
Alan Jesus ◽  
Adriano Jesus ◽  
Flávia Lima ◽  
Luiz Freitas ◽  
Cássio Meira ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Bone Defect ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Osteogenic Potential ◽  
Autogenous Bone ◽  
Control Group

Autogenous bone grafting is needed in some bone tissue defects; however, it causes secondary surgical wounds and morbidity. Tissue bioengineering may be an alternative approach for bone regeneration. Here we investigated the osteogenic potential of dental pulp stem cells from deciduous teeth (DPSC) in association with a Ricinus bone compound (RBC) in a model of bone defect. The influence of the biomaterial RBC on the proliferation and osteogenic differentiation of DPSC was assessed in vitro by MTT metabolism and alizarin red staining, respectively. The morphologic analysis was performed using the optic and scanning electron (SEM) microscopies. For the in vivo study, 54 Wistar rats submitted to calvarial defects were filled with RBC or RBC+DPSC. A control group had the defects filled only with blood clots. Analyses were performed 15, 30 and 60 days after treatment using digital radiography, optical microscopy, SEM and chemical analysis by electron dispersive spectroscopy. The Ricinus bone compound (RBC) did not inhibit the osteogenic differentiation in vitro. No spontaneous regeneration was observed in the control group. The area of the calvarial defect of the RBC+DPSC group showed greater radiopacity on day 15. The RBC presented no reabsorption, was biocompatible and showed osteointegration, working as a mechanical filling. Only sparse ossification areas were found and those were larger and more developed on the RBC+DPSC group when compared to animals treated only with RBC. RBC in association with DPSC is a promising combination for applications in bone regeneration.  

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