scholarly journals A Comparative Evaluation of Different Chemical Agents and Herbal Products in Disinfecting Gutta-Percha Cones: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 36-39
Author(s):  
Manu Bansal ◽  
Rajinder Bansal ◽  
Nikhil Dev Wazir ◽  
Mandeep Singh Matta ◽  
Shalini Chaudhary ◽  
...  
Keyword(s):  
Aloe Vera ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Herbal Products ◽  
Whatman Filter Paper ◽  
Gutta Percha ◽  
Chemical Agents ◽  
Group 2

Abstract Introduction The main aim of this study was to compare and evaluate different chemical agents and herbal products in disinfecting gutta-percha (GP) cones through an in vitro study. Materials and Methods For this study, 160 GP cones were taken in test tubes, which were contaminated with Enterococcus faecalis inocula, prepared by dissolving it in Brain Heart Infusion (BHI) broth. The GP cones were then taken from the test tubes and dried on Whatman filter paper no. 1 and were divided into four groups according to the decontaminant used for the study—group 1 (5% sodium hypochlorite [NaOCl]), group 2 (2% chlorhexidine), group 3 (propolis), and group 4 (Aloe vera)—which were further subdivided into subgroups on the basis immersion time periods of 1 and 10 minutes. The GP cones were then again dried and taken in the test tubes containing freshly prepared BHI broth to check the turbidity. Results It was found that 2% chlorhexidine was the most effective disinfectant against E. faecalis, 5% NaOCl was the second best disinfectant followed by propolis, while Aloe vera had not shown any effect as GP disinfectant. Conclusion Since 2% chlorhexidine showed better disinfection efficacy against E. faecalis than 5% NaOCl, it can be recommended for chairside disinfection of GP. One-minute immersion of GP in 2% chlorhexidine is sufficient for elimination of E. faecalis.

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments

2020 ◽  
Author(s):  
Ahmad Almehmadi
Keyword(s):  
Sodium Hypochlorite ◽  
Bacterial Culture ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Sem Images ◽  
Streptococcus Sanguis ◽  
Implant Dentistry ◽  
Vitro Analysis

Abstract The re-use of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in-vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. 122 HAs (Used and sterilized n=107; New n=15) were procured from 3 centers, of which 3 samples were discarded due to perforation in sterilization pouch.  For sterility assessment, the used HAs (n=80) were cultured in Brain Heart Infusion Broth (BHI) and Potato Dextrose Agar (PDA), bacterial isolates were identified in 7 samples. Also, 24 used HAs were stained with Phloxine B, photographed and compared to new HAs (n=5). Scanning electron microscope (SEM) assessed the differences between the two sets of HAs, following which the 7 contaminated HAs along with 24 used HAs from staining experiment (Total=31) were subsequently treated with sodium hypochlorite (NaOCl) and SEM images were observed. About 8.75% of HAs tested positive in bacterial culture; Streptococcus sanguis, Dermabacter hominis, Staphylococcus haemolyticus, and Aspergillus species were isolated. Phloxine B staining was positive for used and sterilized HAs when compared to controls. The SEM images revealed deposits in the used HAs and although treatment with NaOCl eliminated the contamination of cultured HAs, the SEM showed visible debris in the HA thread region. This in-vitro study concluded that SEM images showed debris in used HAs at screw-hole and thread regions even though they tested negative in bacterial culture. The treatment with NaOCl of used HAs showed no bacterial contamination but the debris was observed in SEM images. Future studies on the chemical composition, biological implications, and clinical influence is warranted before considering the reuse of HAs.

QUALITY CONTROL AND IN-VITRO STUDY RELEASE BEHAVIOUR OF EURYCOMANONE IN HERBAL PRODUCTS SUPPLEMENT IN CAPSULE FORM

2020 ◽  
Vol 24 (04) ◽  
pp. 640-649
Author(s):  
Noorazwani Zainol ◽  
Mohamad Shahrizad Pairon ◽  
Mohd Eeyad Arief Mohd Nor Asri ◽  
Khetiswari Ganesan
Keyword(s):  
Quality Control ◽  
In Vitro Study ◽  
Vitro Study ◽  
Herbal Products

EVALUATION OF THE DISSOLVING EFFICACY OF ESSENTIAL OILS ON ENDODONTIC OBTURATING MATERIALS: AN IN VITRO STUDY.

2021 ◽  
pp. 66-68
Author(s):  
Rakesh Pyla ◽  
Jai Kiran Killada ◽  
V Raja Sekhar
Keyword(s):  
Essential Oils ◽  
Epoxy Resin ◽  
In Vitro Study ◽  
Vitro Study ◽  
Lavender Oil ◽  
Citronella Oil ◽  
Gutta Percha ◽  
Lemon Oil ◽  
The Difference

Aim:Endodontic retreatment is a procedure that removes the lling materials from the root canals followed by their cleaning, shaping and obturation. This in-vitro study aimed to compare and evaluate the ability of various essential oils as solvents in dissolving gutta-percha, epoxy resin, and zinc oxide eugenol (ZOE) cements. Materials and methods: A total of 28 cylindrical specimens in each group ZOE, epoxy resin, 28 ISO size 40 gutta-percha cones were prepared and divided into four groups for immersion in the different solvents, i.e. lemon oil, citronella oil, lavender oil, and TCE(Tetrachloroethylene (control)) for 5 minutes. The obturating materials dissolution in the solvents were obtained by the difference between the pre-immersion original weight and the post-immersion weight on a digital analytical scale. Data were statistically analysed by a paired t-test and Analysis of Variance (ANOVA) (p < 0.05). Results: Order of efcacy of dissolution of essential oils was found to be lemon oil > Citronella oil > Lavender oil and was highly signicant (p < 0.01). In all the solvents, Gutta-percha showed maximum dissolution (Fvalue:149.56) followed by ZOE(89.07 ) and resin sealer least (23.86). Conclusion: It can be concluded that the lemon oil can be used as a solvent for dissolving obturating materials.

Implants With Internal Hexagon and Conical Implant-Abutment Connections: An In Vitro Study of the Bacterial Contamination

2014 ◽  
Vol 40 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Simonetta D'Ercole ◽  
Antonio Scarano ◽  
Vittoria Perrotti ◽  
Jorge Mulatinho ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
Bacterial Contamination ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bacterial Suspension ◽  
Inflammatory Reactions ◽  
Implant Abutment ◽  
Group 2 ◽  
Group 1 ◽  
Internal Connections

Prevention of microbial leakage at the implant-abutment junction is a major challenge for the construction of 2-stage implants in order to minimize inflammatory reactions and to maximize bone stability at the implant neck. The aim of the present in vitro study was an evaluation of the leakage observed over a period of 28 days in Cone Morse taper internal connections and in screwed-abutments connections. In the present study 10 specimens of Cone Morse (Group 1) and 10 of internal hexagon (Group 2) implants were used. The inner parts of 5 implants per group were inoculated with Pseudomonas aeruginosa (PS) suspension and 5 implants per group with Aggregatibacter actinomycetemcomitans (AA). The possible penetration of bacterial suspension into the surrounding solution was determined by the observation of turbidity of the broth. In Group 1, bacterial contamination was found in 3 out of 5 implant-abutment assemblies seeded with the PS and in 2 samples out of 5 in the assemblies seeded with AA, with a total of leaked assemblies in this group of 5 out of 10. In Group 2, bacterial contamination was found in 4 out of 5 implant-abutment assemblies seeded with the PS, and in 4 out of 5 samples seeded with AA, with a total of leaked assemblies of 8 out of 10. The present data confirm the reported high permeability to bacterial leakage of screw-retained abutment connections, and the lower infiltration rates—although not significantly—of Cone Morse taper internal connections.

Photomicrographic evaluation of the apical sealing capacity of three types of gutta-percha master cones: an in vitro study

Odontology ◽  
2011 ◽  
Vol 100 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Luminita Maria Nica ◽  
Andreea Didilescu ◽  
Darian Rusu ◽  
Adrian Bacila ◽  
Stefan-Ioan Stratul
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Sealing Capacity ◽  
Gutta Percha
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