Undercarboxylated Osteocalcin Increases the Dopaminergic Activity of Neuronal Differentiated PC12 Cells In Vitro

A new brominated norsesquiterpene glycoside, acoruside (1), has been isolated from the rhizomes of Acorus tatarinowii Schott, together with 8 known compounds (2-9). Their structures were elucidated mainly based on 1-dimensional (1D) and 2D nuclear magnetic resonance spectra. The absolute configuration of compound 1 was determined by comparing its experimental and calculated electronic circular dichroism spectra. The in vitro tests indicated that at 10 µM, compounds 2, 3, and 4 aggravated serum deprivation injuries of PC12 cells, compound 2 aggravated rotenone-induced injuries of PC12 cells, and compounds 3 and 4 aggravated the oxygen-glucose deprivation-induced injuries of PC12 cells.

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New Cyclic Diarylheptanoids from Platycarya strobilacea

Molecules ◽

10.3390/molecules25246034 ◽

2020 ◽

Vol 25 (24) ◽

pp. 6034

Author(s):

Wen-bing Ding ◽

Rui-yuan Zhao ◽

Guan-hua Li ◽

Bing-lei Liu ◽

Hua-liang He ◽

...

Keyword(s):

Nitric Oxide ◽

Pc12 Cells ◽

Stem Bark ◽

No Production ◽

Electronic Circular Dichroism ◽

Pheochromocytoma Pc12 ◽

Pheochromocytoma Pc12 Cells ◽

Induced Apoptosis ◽

Circular Dichroïsm

Five new cyclic diarylheptanoids (platycary A–E, compounds 1–5) and three previously identified analogues (i.e., phttyearynol (compound 6), myricatomentogenin (compound 7), and juglanin D (compound 8)) were isolated from the stem bark of Platycarya strobilacea. The structures of these compounds were determined using NMR, HRESIMS, and electronic circular dichroism (ECD) data. The cytotoxicity of compounds 1–5 and their ability to inhibit nitric oxide (NO) production, as well as protect against the corticosterone-induced apoptosis of Pheochromocytoma (PC12) cells, were evaluated in vitro using the appropriate bioassays. Compounds 1 and 2 significantly inhibited the corticosterone-induced apoptosis of PC12 cells at a concentration of 20 μΜ.

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Oligomeric Aβ affects cell function in NGF-differentiated PC12 cells

Neurobiology of Aging ◽

10.1016/s0197-4580(00)82734-7 ◽

2000 ◽

Vol 21 ◽

pp. 13

Author(s):

Linda M. Rowse ◽

M. Desiree Watson ◽

Glenn K. Walker ◽

Mark R. Emmerling ◽

Harry X. LeVine ◽

...

Keyword(s):

Pc12 Cells ◽

Cell Function ◽

Differentiated Pc12 Cells

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Synaptotagmin IV is necessary for the maturation of secretory granules in PC12 cells

The Journal of Cell Biology ◽

10.1083/jcb.200506163 ◽

2006 ◽

Vol 173 (2) ◽

pp. 241-251 ◽

Cited By ~ 53

Author(s):

Malika Ahras ◽

Grant P. Otto ◽

Sharon A. Tooze

Keyword(s):

Pc12 Cells ◽

Secretory Granules ◽

Granule Formation ◽

Synaptotagmin Iv ◽

Prohormone Convertase 2 ◽

Granule Maturation ◽

Golgi Network ◽

Interfering Rna

In neuroendocrine PC12 cells, immature secretory granules (ISGs) mature through homotypic fusion and membrane remodeling. We present evidence that the ISG-localized synaptotagmin IV (Syt IV) is involved in ISG maturation. Using an in vitro homotypic fusion assay, we show that the cytoplasmic domain (CD) of Syt IV, but not of Syt I, VII, or IX, inhibits ISG homotypic fusion. Moreover, Syt IV CD binds specifically to ISGs and not to mature secretory granules (MSGs), and Syt IV binds to syntaxin 6, a SNARE protein that is involved in ISG maturation. ISG homotypic fusion was inhibited in vivo by small interfering RNA–mediated depletion of Syt IV. Furthermore, the Syt IV CD, as well as Syt IV depletion, reduces secretogranin II (SgII) processing by prohormone convertase 2 (PC2). PC2 is found mostly in the proform, suggesting that activation of PC2 is also inhibited. Granule formation, and the sorting of SgII and PC2 from the trans-Golgi network into ISGs and MSGs, however, is not affected. We conclude that Syt IV is an essential component for secretory granule maturation.

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