Detection of early neoplasia in Barrett’s esophagus using lectin-based near-infrared imaging: an ex vivo study on human tissue

Abstract Background and study aims Endoscopic surveillance for Barrett’s esophagus (BE) is limited by long procedure times and sampling error. Near-infrared (NIR) fluorescence imaging minimizes tissue autofluorescence and optical scattering. We assessed the feasibility of a topically applied NIR dye-labeled lectin for the detection of early neoplasia in BE in an ex vivo setting. Methods Consecutive patients undergoing endoscopic mucosal resection (EMR) for BE-related early neoplasia were recruited. Freshly collected EMR specimens were sprayed at the bedside with fluorescent lectin and then imaged. Punch biopsies were collected from each EMR under NIR light guidance. We compared the fluorescence intensity from dysplastic and nondysplastic areas within EMRs and from punch biopsies with different histological grades. Results 29 EMR specimens were included from 17 patients. A significantly lower fluorescence was found for dysplastic regions across whole EMR specimens (P < 0.001). We found a 41 % reduction in the fluorescence of dysplastic compared to nondysplastic punch biopsies (P < 0.001), with a sensitivity and specificity for dysplasia detection of 80 % and 82.9 %, respectively. Conclusion Lectin-based NIR imaging can differentiate dysplastic from nondysplastic Barrett’s mucosa ex vivo.

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Assessment of ex-vivo and in-vivo near-infrared Raman spectroscopy for the classification of dysplasia within Barrett's esophagus

10.1117/12.384963 ◽

2000 ◽

Cited By ~ 4

Author(s):

Martin G. Shim ◽

Louis-Michel Wong Kee Song ◽

Norman E. Marcon ◽

Shirley Hassaram ◽

Brian C. Wilson

Keyword(s):

Raman Spectroscopy ◽

Barrett’S Esophagus ◽

Barrett's Esophagus ◽

Near Infrared ◽

Ex Vivo

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Liposomal Chitosan-N-Acetyl Cysteine Nanoparticle for In-Vitro/In-Vivo Near Infrared Fluorescent (NIR) Imaging

10.26434/chemrxiv.9976145.v1 ◽

2019 ◽

Author(s):

Piyush Kumar ◽

Timothy Van Treuren ◽

Amalendu Ranjan ◽

Jamboor K Vishwanatha

Keyword(s):

Near Infrared ◽

Infrared Imaging ◽

Tumor Imaging ◽

Ex Vivo ◽

Breast Cancer Cell Lines ◽

Nir Imaging ◽

Negative Surface ◽

Acetyl Cysteine

<p>In this work, we studied the effect of chitosan conjugated N-acetyl cysteine (CHT-NAC) coating on liposomal (DMPC:14 lysoPG: DSPE-2000-NH2) nanoparticles as a vehicle to cross the blood-brain barrier. The size of lipo-NP and Lipo-CHT-NAC NP were sub 50 nm with negative surface charge consistent with its use in an intravenous application. In vitro near infrared (NIR) imaging showed good cellular uptake in two triple-negative breast cancer cell lines (MDA-MB-231 and brain metastatic MDA-MB-831). Live (4-120 h) and ex-vivo near-infrared imaging at 24 h in nude mice showed the extended circulation of CHT-NAC Lipo-NP. These results demonstrated that Lipo-CHT-NAC NP could be used for metastatic brain tumor imaging.</p>

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Liposomal Chitosan-N-Acetyl Cysteine Nanoparticle for In-Vitro/In-Vivo Near Infrared Fluorescent (NIR) Imaging

10.26434/chemrxiv.9976145 ◽

2019 ◽

Author(s):

Piyush Kumar ◽

Timothy Van Treuren ◽

Amalendu Ranjan ◽

Jamboor K Vishwanatha

Keyword(s):

Near Infrared ◽

Infrared Imaging ◽

Tumor Imaging ◽

Ex Vivo ◽

Breast Cancer Cell Lines ◽

Nir Imaging ◽

Negative Surface ◽

Acetyl Cysteine

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Optical Detection and Eradication of Dysplastic Barrett's Esophagus

Technology in Cancer Research & Treatment ◽

10.1177/153303460300200403 ◽

2003 ◽

Vol 2 (4) ◽

pp. 289-302 ◽

Cited By ~ 7

Author(s):

Louis-Michel Wong Kee Song ◽

Kenneth K. Wang

Keyword(s):

Barrett’S Esophagus ◽

Barrett's Esophagus ◽

Sampling Error ◽

Imaging Techniques ◽

Standard Of Care ◽

Diagnostic Techniques ◽

Endoscopic Surveillance ◽

Current Status ◽

Delay In Diagnosis ◽

Multiple Biopsies

Dysplastic Barrett's esophagus is a condition that offers multiple diagnostic and therapeutic challenges. The diagnosis of dysplasia within Barrett's esophagus currently relies on periodic endoscopic surveillance with multiple biopsies, a methodology limited by random sampling error, inconsistent histopathologic interpretation and delay in diagnosis. Optical spectroscopic and imaging techniques have the potential to identify dysplastic or early neoplastic lesions in real-time. These diagnostic modalities are needed to enhance the endoscopic surveillance of Barrett's esophagus in the future as well as help to define lesions for endoscopic therapy. Esophagectomy has been the standard of care for Barrett's esophagus with high-grade dysplasia although it is a procedure associated with significant morbidity and mortality. Minimally invasive endoscopic ablative therapies are attractive and less morbid alternatives to esophagectomy, with promising results obtained from the use of light-activated drugs (i.e., photodynamic therapy). The combination of novel optical diagnostic techniques and therapies will provide the endoscopist with much needed tools that can considerably enhance the management of patients with Barrett's esophagus. This article reviews the current status and future prospects of optical-based modalities for diagnosis and therapy of dysplastic Barrett's esophagus.

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Methylene Blue Staining of Dysplastic and Nondysplastic Barrett's Esophagus: An In Vivo and Ex Vivo Study

Endoscopy ◽

10.1055/s-2001-14427 ◽

2001 ◽

Vol 33 (5) ◽

pp. 391-400 ◽

Cited By ~ 103

Author(s):

M. I. Canto ◽

S. Setrakian ◽

J. E. Willis ◽

A. Chak ◽

R. E. Petras ◽

...

Keyword(s):

Methylene Blue ◽

Barrett’S Esophagus ◽

Barrett's Esophagus ◽

Ex Vivo ◽

Blue Staining ◽

Ex Vivo Study

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In-vivo near-infrared optical imaging of growing osteosarcoma cell lesions xenografted in mice: dual-channel quantitative evaluation of volume and mineralization

Acta Radiologica ◽

10.1258/ar.2011.110131 ◽

2011 ◽

Vol 52 (9) ◽

pp. 978-988 ◽

Cited By ~ 8

Author(s):

Hitoshi Nakayama ◽

Tomoyuki Kawase ◽

Kazuhiro Okuda ◽

Larry F Wolff ◽

Hiromasa Yoshie

Keyword(s):

Optical Imaging ◽

Near Infrared ◽

Imaging Technique ◽

Ex Vivo ◽

Acquisition Time ◽

Osteosarcoma Cell ◽

Dual Channel ◽

Nir Imaging ◽

Ex Vivo Imaging

Background In a previous study using a rodent osteosarcoma-grafted rat model, in which cell-dependent mineralization was previously demonstrated to proportionally increase with growth, we performed a quantitative analysis of mineral deposit formation using 99mTc-HMDP and found some weaknesses, such as longer acquisition time and narrower dynamic ranges (i.e. images easily saturated). The recently developed near-infrared (NIR) optical imaging technique is expected to non-invasively evaluate changes in living small animals in a quantitative manner. Purpose To test the feasibility of NIR imaging with a dual-channel system as a better alternative for bone scintigraphy by quantitatively evaluating mineralization along with the growth of osteosarcoma lesions in a mouse-xenograft model. Material and Methods The gross volume and mineralization of osteosarcoma lesions were evaluated in living mice simultaneously with dual-channels by NIR dye-labeled probes, 2-deoxyglucose (DG) and pamidronate (OS), respectively. To verify these quantitative data, retrieved osteosarcoma lesions were then subjected to ex-vivo imaging, weighing under wet conditions, microfocus-computed tomography (μCT) analysis, and histopathological examination. Results Because of less scattering and no anatomical overlapping, as generally shown, specific fluorescence signals targeted to the osteosarcoma lesions could be determined clearly by ex-vivo imaging. These data were well positively correlated with the in-vivo imaging data ( r > 0.8, P < 0.02). Other good to excellent correlations ( r > 0.8, P < 0.02) were observed between DG accumulation and tumor gross volume and between OS accumulation and mineralization volume. Conclusion This in-vivo NIR imaging technique using DG and OS is sensitive to the level to simultaneously detect and quantitatively evaluate the growth and mineralization occuring in this type of osteosarcoma lesions of living mice without either invasion or sacrifice. By possible mutual complementation, this dual imaging system might be useful for accurate diagnosis even in the presence of overlapping tissues.

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Deep tissue near-infrared imaging for vascular network analysis

Journal of Innovative Optical Health Sciences ◽

10.1142/s1793545816500516 ◽

2017 ◽

Vol 10 (03) ◽

pp. 1650051 ◽

Cited By ~ 3

Author(s):

Kübra Seker ◽

Mehmet Engin

Keyword(s):

Network Analysis ◽

Near Infrared ◽

Infrared Imaging ◽

Imaging System ◽

Hemoglobin Concentration ◽

Cost Effective ◽

Vascular Network ◽

Vascular Imaging ◽

Nir Light ◽

Optical Region

Subcutaneous vein network plays important roles to maintain microcirculation that is related to some diagnostic aspects. Despite developments of optical imaging technologies, still the difficulties about deep skin vascular imaging have been continued. On the other hand, since hemoglobin concentration of human blood has key role in the veins imaging by optical manner, the used wavelength in vascular imaging, must be chosen considering absorption of hemoglobin. In this research, we constructed a near infrared (NIR) light source because of lower absorption of hemoglobin in this optical region. To obtain vascular image, reflectance geometry was used. Next, from recorded images, vascular network analysis, such as calculation of width of vascular of interest and complexity of selected region were implemented. By comparing with other modalities, we observed that proposed imaging system has great advantages including nonionized radiation, moderate penetration depth of 0.5–3[Formula: see text]mm and diameter of 1[Formula: see text]mm, cost-effective and algorithmic simplicity for analysis.

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Folate Receptor Near-Infrared Optical Imaging Provides Sensitive and Specific Intraoperative Visualization of Nonfunctional Pituitary Adenomas

Operative Neurosurgery ◽

10.1093/ons/opy034 ◽

2018 ◽

Vol 16 (1) ◽

pp. 59-70 ◽

Cited By ~ 8

Author(s):

Steve S Cho ◽

Ryan Zeh ◽

John T Pierce ◽

Jun Jeon ◽

MacLean Nasrallah ◽

...

Keyword(s):

Predictive Value ◽

Pituitary Adenomas ◽

Near Infrared ◽

Folate Receptor ◽

P Value ◽

Fluorescence Signal ◽

Postoperative Mri ◽

Nir Fluorescence ◽

Residual Neoplasm ◽

Nir Dye

Abstract BACKGROUND Surgical resection is the primary treatment for nonfunctional (NF) pituitary adenomas, but gross-total resection is difficult to achieve in all cases. NF adenomas overexpress folate receptor alpha (FRα). OBJECTIVE To test the hypothesis that we could target FRα for highly sensitive and specific intraoperative detection of NF adenomas using near-infrared (NIR) imaging. METHODS Fourteen patients with NF pituitary adenoma were infused with the folate analog NIR dye OTL38 preoperatively. NIR fluorescence signal-to-background ratio (SBR) was recorded for each tumor during resection of the adenomas. Extent of surgery was not modified based on the presence or absence of fluorescence. Immunohistochemistry was performed to assess FRα expression in all specimens. Magnetic resonance imaging (MRI) was performed postoperatively to assess residual neoplasm. RESULTS Nine adenomas overexpressed FRα and fluoresced with a NIR SBR of 3.2 ± 0.52, whereas the 5 non-FRα-overexpressing adenomas fluoresced with an SBR of 1.5 ± 0.21. Linear regression demonstrated a significant correlation between intraoperative SBR and the FRα expression (P-value < .001). Analysis of 14 margin samples revealed that the surgeon's impression of the tissue had 83% sensitivity, 100% specificity, 100% positive predictive value, and 89% negative predictive value, while NIR fluorescence had 100% for all values. NIR fluorescence accurately predicted postoperative MRI results in 78% of FRα-overexpressing patients. CONCLUSION Preoperative injection of folate-tagged NIR dye provides strong signal and visualization of NF pituitary adenomas. It is 100% sensitive and specific for detecting margin neoplasm and can predict postoperative MRI findings. Our results suggest that NIR fluorescence may be superior to white-light visualization alone and may improve resection rates in NF pituitary adenomas.

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Clinical use of organic near-infrared fluorescent contrast agents in image-guided oncologic procedures and its potential in veterinary oncology

Veterinary Record ◽

10.1136/vr.104851 ◽

2018 ◽

Vol 183 (11) ◽

pp. 354-354 ◽

Cited By ~ 6

Author(s):

Sophie Favril ◽

Eline Abma ◽

Francesco Blasi ◽

Emmelie Stock ◽

Nausikaa Devriendt ◽

...

Keyword(s):

Fluorescence Imaging ◽

Fluorescent Probes ◽

Near Infrared ◽

Sentinel Lymph Node Mapping ◽

Cost Effective ◽

Surgical Oncology ◽

Great Promise ◽

Lymph Node Mapping ◽

Nir Light ◽

Nir Fluorescence

One of the major challenges in surgical oncology is the intraoperative discrimination of tumoural versus healthy tissue. Until today, surgeons rely on visual inspection and palpation to define the tumoural margins during surgery and, unfortunately, for various cancer types, the local recurrence rate thus remains unacceptably high. Near-infrared (NIR) fluorescence imaging is an optical imaging technique that can provide real-time preoperative and intraoperative information after administration of a fluorescent probe that emits NIR light once exposed to a NIR light source. This technique is safe, cost-effective and technically easy. Several NIR fluorescent probes are currently studied for their ability to highlight neoplastic cells. In addition, NIR fluorescence imaging holds great promise for sentinel lymph node mapping. The aim of this manuscript is to provide a literature review of the current organic NIR fluorescent probes tested in the light of human oncology and to introduce fluorescence imaging as a valuable asset in veterinary oncology.

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