Background::
Panax Notoginseng Saponins (PNS) is used as traditional Chinese medicine for ischemic stroke and cardiovascular
disease, it has been proven to possess anticancer activity recently.
Objective::
In this study, we aimed to explore the anticancer curative effect and potential mechanisms of PNS in pancreatic cancer cells.
Methods::
Pancreatic cancer Miapaca2 and PANC-1 cells were treated with PNS and Gemcitabine (Gem), respectively. Then the cell
viability was assessed by CCK-8 assay, cell proliferation was tested by colony formation assay and EdU cell proliferation assay, cell
migration and invasiveness were tested by wound healing assay and transwell assay respectively, and cell apoptosis was detected by flow
cytometry. Finally, we detected the expression levels of proteins related to migration, apoptosis and autophagy through Western blotting.
Results::
PNS not only inhibited the proliferation, migration, invasion and autophagy of Miapaca2 and PANC-1 cells, but also induced
apoptosis and promoted chemosensitivity of pancreatic cancer cells to Gem.
Conclusion::
PNS may exhibit cytotoxicity and increase chemosensitivity of pancreatic cancer cells to Gem by inhibiting autophagy and
inducing apoptosis, providing a new strategy and potential treatment option for pancreatic cancer.
Beclin1 inhibition promotes autophagy and decreases gemcitabine–induced apoptosis in Miapaca2 pancreatic cancer cells
