Tc-99m Tetrofosmin Brain SPECT for the Assessment of Meningiomas. Correlation with Histological Grade and MIB-1 Proliferation Index

Alteration of β-catenin expression is involved in the development and evolution of hepatocellular carcinoma (HCC); β-catenin is able to influence tumor cell proliferation. We analyzed the immunohistochemical (IHC) expression of β-catenin on a group of 32 patients diagnosed with HCC using the anti-β-catenin monoclonal antibody (clone E247). We correlated the expression of β-catenin with the proliferation index of Ki-67 (PI Ki-67), the mitotic index (MI) and other clinical and pathological features. We observed an altered β-catenin expression in 58.38% of all HCC cases. This expression was insignificantly correlated with tumor size (]5 cm) (p = 0.683), histological grade G1-G2 (p = 0.307), vascular invasion (p = 0.299) and advanced pT stage (p = 0.453); we obtained a significantly higher MI in HCC with altered β-catenin expression (p = 0.018), as compared to HCC without overexpression (1.66 � 1.37) (p = 0.038) and a PI Ki-67 of 22.49 � 20.1 and 28.24 � 18.2, respectively in tumors with altered β-catenin expression with insignificant differences compared to HCC without overexpression (25.95 � 15.2) (p = 0.682 and p = 0.731, respectively). According to the results we obtained, aberrant β-catenin expression in HCC was correlated with a high mitotic index, therefore playing an important role in tumor progression by stimulating tumor cell proliferation; non-nuclear β-catenin overexpression can have a pathological significance in HCC, especially in cases of HCC associated with hepatitis B virus (HBV) infection.

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Noninvasive Carcinoma of the Breast: Angiogenesis and Cell Proliferation

Archives of Pathology & Laboratory Medicine ◽

10.5858/2004-128-893-ncotba ◽

2004 ◽

Vol 128 (8) ◽

pp. 893-896 ◽

Cited By ~ 1

Author(s):

Ying Cao ◽

Gladell P. Paner ◽

Leonard B. Kahn ◽

Prabha B. Rajan

Keyword(s):

Cell Proliferation ◽

Microvessel Density ◽

Carcinoma In Situ ◽

Nuclear Grade ◽

Proliferation Index ◽

Carcinoma Of The Breast ◽

Cell Proliferation Index ◽

Noninvasive Carcinoma ◽

Mib 1

Abstract Context.—Angiogenesis and the cell proliferation index can predict the prognosis of invasive breast carcinoma; however, little is known of their roles in noninvasive tumor. Objective.—To investigate the correlation of microvessel density and cell proliferation index with other histologic parameters (histologic type, nuclear grade, and mitotic count) in 65 cases of noninvasive carcinoma of the breast. Design.—Formalin-fixed, paraffin-embedded tissues from 65 cases of carcinoma in situ of the breast were immunostained with antibody against factor VIII antigen and proliferation-associated nuclear antigen MIB-1. The microvessel density was measured by counting the total number of microvessels around the carcinoma in situ per 10 low-power microscopic fields. The cell proliferation index was calculated by counting MIB-1–positive nuclei in 100 tumor cells. A χ2 test and Spearman rank correlation test were used for statistical analysis. Results.—The microvessel density and cell proliferation index of comedo-type, high-nuclear-grade ductal carcinomas in situ are significantly higher than those of either noncomedo type ductal carcinomas in situ or lobular carcinoma in situ (P < .001). Conclusions.—Angiogenesis and the cell proliferation index are active biological processes and may be considered as markers to separate low- and high-risk patients with noninvasive breast carcinomas.

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Medulloblastomas: a correlative study of MIB-1 proliferation index along with expression of c-Myc, ERBB2, and anti-apoptotic proteins along with histological typing and clinical outcome

Child s Nervous System ◽

10.1007/s00381-009-0884-9 ◽

2009 ◽

Vol 25 (7) ◽

pp. 825-835 ◽

Cited By ~ 12

Author(s):

Prasenjit Das ◽

Tarun Puri ◽

Vaishali Suri ◽

M. C. Sharma ◽

B. S. Sharma ◽

...

Keyword(s):

Clinical Outcome ◽

Proliferation Index ◽

Correlative Study ◽

Histological Typing ◽

Apoptotic Proteins ◽

Mib 1

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Clinicopathological significance of WT1 expression in invasive breast carcinoma with >90% mucinous component

Journal of Clinical Pathology ◽

10.1136/jclinpath-2021-207464 ◽

2021 ◽

pp. jclinpath-2021-207464

Author(s):

Xiaoli Xu ◽

Rui Bi ◽

Ruohong Shui ◽

Baohua Yu ◽

Yufan Cheng ◽

...

Keyword(s):

Breast Carcinoma ◽

Growth Pattern ◽

Histological Grade ◽

Invasive Breast Carcinoma ◽

Nuclear Grade ◽

Proliferation Index ◽

Her2 Overexpression ◽

Ki 67 ◽

Mucinous Component ◽

Clinicopathological Significance

AimsThis study was aimed to investigate the clinicopathological significance of immunohistochemical (IHC) Wilm’s tumour 1 (WT1) expression in invasive breast carcinoma with >90% mucinous components.MethodsOne hundred specimens of invasive breast carcinoma with >90% mucinous component were collected. All H&E-stained slides were reviewed, and the clinicopathological data, including sex, age, tumour size, nuclear grade, histological grade, growth pattern and lymph node (LN) status, were collected. IHC staining of WT1, oestrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and Ki-67 was performed. Fluorescence in situ hybridisation was used to verify the amplification of the HER2 gene. The relationship between WT1 expression and clinicopathological features was analysed statistically.ResultsWT1 expression was detected in 67% (67/100) of invasive breast carcinoma with >90% mucinous components. WT1 expression was significantly associated with low-to-intermediate nuclear grade/histological grade, ER and PR positivity, HER2 negativity, Ki-67 proliferation index <30% and noLN metastasis (all p<0.001). Micropapillary architecture was observed in 80% of cases. WT1 expression was not significantly correlated with different percentage of micropapillary components (p=0.422). None of the histological grade 3 tumours, tumours with HER2 overexpression/amplification and triple-negative specimens showed WT1 expression.ConclusionsWT1 expression was significantly related with low-intermediate nuclear/histological grade, ER positivity, HER2 negativity, a lower Ki-67 proliferation index and no LN metastasis in invasive breast carcinoma with >90% mucinous component. The micropapillary growth pattern in this type of tumour did not show a specific relationship with WT1 expression.

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Prognostic value of MIB-1 proliferation index in solitary fibrous tumors of the pleura implemented in a new score – a multicenter study

Respiratory Research ◽

10.1186/s12931-017-0693-8 ◽

2017 ◽

Vol 18 (1) ◽

Cited By ~ 11

Author(s):

Matthias Diebold ◽

Alex Soltermann ◽

Selma Hottinger ◽

Sarah R. Haile ◽

Lukas Bubendorf ◽

...

Keyword(s):

Multicenter Study ◽

Prognostic Value ◽

Proliferation Index ◽

Solitary Fibrous Tumors ◽

Mib 1

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Medulloblastoma: evaluation of proliferative index by monoclonal antibody Mib-1, its prognostic correlation and therapeutic implications

Arquivos de Neuro-Psiquiatria ◽

10.1590/s0004-282x2003000400004 ◽

2003 ◽

Vol 61 (3A) ◽

pp. 547-551 ◽

Cited By ~ 4

Author(s):

Antonio Fernandes Ferrari ◽

Maria Betânia Mahler Araújo ◽

Paulo Henrique Aguiar ◽

José Pindaro Pereira Plese

Keyword(s):

Monoclonal Antibody ◽

Prognostic Value ◽

Cellular Proliferation ◽

Proliferation Index ◽

Histopathological Diagnosis ◽

Central Nervous System Tumor ◽

Proliferative Index ◽

The Mean ◽

Monoclonal Antibody Mib 1 ◽

Mib 1

In the past few years, the monoclonal antibody MIB-1 has been used by researchers in order to retrospectively study paraffin imbibed tumor fragments. The medulloblastoma is the most common malignant central nervous system tumor in childhood. The objectives were: determination of the mean Mib-1 LI value from these patients, as well as the prognostic value of the method.This retrospective study represents an analysis of the cellular proliferation index of posterior fossa medulloblastomas collected from 22 patients at A.C. Camargo Hospital, from January 1990 to December 1999. The histopathological diagnosis was confirmed by H&E and proliferative index (LI) was achived with Mib-1 which detects proliferating cells during G1, G2, S and M phases.The results demostrated that the mean Mib-1 was 30,1%, and ranged from 5,2% to 62,0%.In conclusion, this method has prognostic value, has to be used as routine for patients harboring medulloblastomas and the ones who have PI greater than the mean value found in this study, should be treated aggressively.

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MIB-1 proliferation index correlates with survival in pleural malignant mesothelioma

Histopathology ◽

10.1046/j.1365-2559.2000.00793.x ◽

2000 ◽

Vol 36 (1) ◽

pp. 26-31 ◽

Cited By ~ 25

Author(s):

Comin ◽

Anichini ◽

Boddi ◽

Novelli ◽

Dini

Keyword(s):

Malignant Mesothelioma ◽

Proliferation Index ◽

Pleural Malignant Mesothelioma ◽

Mib 1

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Assessment of Proliferation Index with MIB-1 as a Prognostic Factor in Radiation Therapy for Cervical Cancer

Gynecologic Oncology ◽

10.1006/gyno.2000.5950 ◽

2000 ◽

Vol 79 (2) ◽

pp. 300-304 ◽

Cited By ~ 7

Author(s):

Mitsuaki Suzuki ◽

Shizuka Tsukagoshi ◽

Yasushi Saga ◽

Michitaka Ohwada ◽

Ikuo Sato

Keyword(s):

Cervical Cancer ◽

Radiation Therapy ◽

Prognostic Factor ◽

Proliferation Index ◽

Mib 1

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Qualitative analysis of spinal intramedullary lesions using PET/CT

Journal of Neurosurgery Spine ◽

10.3171/2015.2.spine141254 ◽

2015 ◽

Vol 23 (5) ◽

pp. 613-619 ◽

Cited By ~ 12

Author(s):

Kentaro Naito ◽

Toru Yamagata ◽

Hironori Arima ◽

Junya Abe ◽

Naohiro Tsuyuguchi ◽

...

Keyword(s):

Fdg Pet ◽

Proliferation Index ◽

Low Grade ◽

Tumor Proliferation ◽

High Grade ◽

Pet Ct ◽

Grade Malignancy ◽

High Grade Malignancy ◽

Met Pet ◽

Mib 1

OBJECT Although the usefulness of PET for brain lesions has been established, few reports have examined the use of PET for spinal intramedullary lesions. This study investigated the diagnostic utility of PET/CT for spinal intramedullary lesions. METHODS l-[methyl-11C]-methionine (MET)- or [18F]-fluorodeoxyglucose (FDG)-PET/CT was performed in 26 patients with spinal intramedullary lesions. The region of interest (ROI) within the spinal cord parenchyma was placed manually in the axial plane. Maximum pixel counts in the ROIs were normalized to the maximum standardized uptake value (SUVmax) using subject body weight. For FDG-PET the SUVmax was corrected for lean body mass (SULmax) to exclude any influence of the patient’s body shape. Each SUV was analyzed based on histopathological results after surgery. The diagnostic validity of the SUV was further compared with the tumor proliferation index using the MIB-1 monoclonal antibody (MIB-1 index). RESULTS A total of 16 patients underwent both FDG-PET and MET-PET, and the remaining 10 patients underwent either FDG-PET or MET-PET. Pathological diagnoses included high-grade malignancy such as glioblastoma multiforme, anaplastic astrocytoma, or anaplastic ependymoma in 5 patients; low-grade malignancy such as hemangioblastoma, diffuse astrocytoma, or ependymoma in 12 patients; and nonneoplastic lesion including cavernous malformation in 9 patients. Both FDG and MET accumulated significantly in high-grade malignancy, and the SULmax and SUVmax correlated with the tumor proliferation index. Therapeutic response after chemotherapy or radiation in high-grade malignancy was well monitored. However, a significant difference in SULmax and SUVmax for FDG-PET and MET-PET was not evident between low-grade malignancy and nonneoplastic lesions. CONCLUSIONS Spinal PET/CT using FDG or MET for spinal intramedullary lesions appears useful and practical, particularly for tumors with high-grade malignancy. Differentiation of tumors with low-grade malignancy from nonneoplastic lesions may still prove difficult. Further technological refinement, including the selection of radiotracer or analysis evaluation methods, is needed.

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