Seasonal and Post-harvest Accumulation of Artemisinin, Artemisinic Acid, and Dihydroartemisinic Acid in Three Accessions of Artemisia annua Cultivated in West Virginia, USA

Planta Medica ◽  
2008 ◽  
Vol 74 (03) ◽  
Author(s):  
JFS Ferreira
Keyword(s):  
West Virginia ◽  
Artemisia Annua ◽  
Post Harvest ◽  
Artemisinic Acid ◽  
Dihydroartemisinic Acid

Lipid Transfer Proteins (AaLTP3 and AaLTP4) Are Involved in Sesquiterpene Lactone Secretion from Glandular Trichomes in Artemisia annua

2019 ◽  
Vol 60 (12) ◽  
pp. 2826-2836 ◽  
Author(s):  
Prakash Babu Adhikari ◽  
Jung Yeon Han ◽  
Chang Ho Ahn ◽  
Yong Eui Choi
Keyword(s):  
Fluorescent Protein ◽  
Artemisia Annua ◽  
Sesquiterpene Lactones ◽  
Glandular Trichomes ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Artemisinic Acid ◽  
Enhanced Production ◽  
Strong Positive Signal ◽  
Dihydroartemisinic Acid

Abstract In Artemisia annua plants, glandular trichomes (GTs) are responsible for the biosynthesis and secretion of sesquiterpene lactones including artemisinin/arteannuin B. Nonspecific lipid transfer proteins (LTPs) in plants bind and carry lipid molecules across the cell membrane and are also known as secretary proteins. Interestingly, the transcripts of LTP genes are exceptionally abundant in the GTs of A. annua. In the present study, we isolated two trichome-specific LTP genes (AaLTP3 and AaLTP4) from a Korean ecotype of A. annua. AaLTP3 was expressed abundantly in shoots, whereas AaLTP4 was expressed in flowers. The GUS signal driven by the AaLTP3 or AaLTP4 promoter in transgenic A. annua plants revealed that the AaLTP3 promoter was active on hair-like non-GTs and that the AaLTP4 promoter was active on GTs. Analysis of enhanced cyan fluorescent protein (ECFP) fluorescence fused with the AaLTP3 or AaLTP4 protein in transgenic tobacco revealed that ECFP florescence was very bright on secreted lipids of long GTs. Moreover, the florescence was also bright on the head cells of short trichomes and their secreted granules. Immunoblotting analysis of GT exudates in petioles of A. annua revealed a strong positive signal against the AaLTP4 antibody. Overexpression of AaLTP3 or AaLTP4 in transgenic A. annua plants resulted in enhanced production of sesquiterpene lactones (arteannuin B, artemisinin, dihydroartemisinic acid and artemisinic acid) compared with those of wild type. The present study shows that LTP genes (AaLTP3 or AaLTP4) play important roles in the sequestration and secretion of lipids in GTs of A. annua, which is useful for the enhanced production of sesquiterpene lactones by genetic engineering.

scholarly journals Preparation and Application of Reversed Phase ChromatorotorTM for the Isolation of Natural Products by Centrifugal Preparative Chromatography

2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Ilias Muhammad ◽  
Volodymyr Samoylenko ◽  
Francis Machumi ◽  
Mohamed Ahmed Zaki ◽  
Rabab Mohammed ◽  
...  
Keyword(s):  
Natural Products ◽  
Artemisia Annua ◽  
Polar Compounds ◽  
Reversed Phase ◽  
Preparative Chromatography ◽  
Planar Chromatography ◽  
Sorbent Layer ◽  
Preparative Scale ◽  
Artemisinic Acid ◽  
Binder Free

A method of preparation of rotors with a reversed phase (RP) solid silica gel sorbent layer has been developed for centrifugal preparative chromatography (CPC), also known as rotational planar chromatography (RPC). The rotors consist of binder free RP solid SiO2 layers of different thicknesses packed between two supported circular glass discs and can be used in any appropriate device for centrifugal chromatography, like Chromatotron® and CycloGraph®. Polar and /or semi-polar compounds with close R f values, as well as extracts and column fractions were separated and /or purified in a preparative and / or semi-preparative scale using the RP rotors, eluted with mixtures of aqueous-based solvents. We herein report three examples of its application, using RP ChromatorotorsTM, for the isolation of the diastereoisomeric alkaloids banistenosides I and II from Banisteriopsis caapi, saponins III and IV from Fagonia cretica, and the sesquiterpenes artemisinin (V) and artemisinic acid (VI) from Artemisia annua.

scholarly journals DMSO triggers the generation of ROS leading to an increase in artemisinin and dihydroartemisinic acid in Artemisia annua shoot cultures

2009 ◽  
Vol 29 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Abdul Mannan ◽  
Chunzhao Liu ◽  
Patrick R. Arsenault ◽  
Melissa J. Towler ◽  
Dan R. Vail ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Shoot Cultures ◽  
Dihydroartemisinic Acid

scholarly journals A New Sesquiterpene and other Constituents from Saussurea Lappa Root

2010 ◽  
Vol 5 (10) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Jin-ao Duan ◽  
Pengfei Hou ◽  
Yuping Tang ◽  
Pei Liu ◽  
Shulan Su ◽  
...  
Keyword(s):  
Acid Derivative ◽  
Artemisia Annua ◽  
Saussurea Lappa ◽  
Artemisinic Acid ◽  
Structural Skeleton ◽  
Dehydrocostus Lactone

Five sesquiterpenes, dehydrocostus lactone (1), santamarine (5), β-cyclocostunolide (6), 4α-hydroxy-4β-methyldihydrocostol (7) and 10α-hydroxyl-artemisinic acid (9), along with four other compounds, β-sitosterol (2), daucosterol (3), 5-hydroxy-methyl-furaldehyde (4), and trans-syingin (8), were isolated and identified from the roots of Saussurea lappa (Compositae). Based on previous reports and our study, sesquiterpene derivatives are common and characteristic constituents of the genus Saussurea. Among the nine compounds obtained, 9 is a new sesquiterpene. It is an artemisinic acid derivative, whose structural skeleton has not been reported for Saussurea species before, but artemisinic acid is a common compound in another Compositae species, Artemisia annua. Dehydrocostus lactone (1) is present in high-content and is a possible bioprecursor of 10α-hydroxyartemisinic acid (9).

scholarly journals Simultaneous Quantification of Five Sesquiterpene Components after Ultrasound Extraction in Artemisia annua L. by an Accurate and Rapid UPLC–PDA Assay

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1530 ◽  
Author(s):  
Jiaqi Ruan ◽  
Zhengyue Liu ◽  
Feng Qiu ◽  
Henan Shi ◽  
Manyuan Wang
Keyword(s):  
Artemisia Annua ◽  
Correlation Coefficients ◽  
Ultra Performance Liquid Chromatography ◽  
Artemisinic Acid ◽  
Artemisia Annua L ◽  
Acid Aqueous Solution ◽  
Photodiode Array ◽  
The Stability ◽  
Acquity Uplc

Objective: To develop an accurate and rapid ultra-performance liquid chromatography (UPLC) coupled with a photodiode array (PDA) method for the simultaneous determination of artemisinin (Art), arteannuin B (Art B), arteannuin C (Art C), dihydroartemisinic acid (DHAA) and artemisinic acid (AA) in Artemisia annua L. Methodology: Chromatography separation was performed on an ACQUITY UPLC BEH C18 Column with isocratic elution; the mobile phase was 0.1% formic acid aqueous solution (A) and acetonitrile (B) (A:B = 40:60, v/v). Data were recorded at an ultraviolet (UV) wavelength of 191 nm for Art, Art C, DHAA and AA, and 206 nm for Art B. Results: The calibration curves of the five sesquiterpene components were all linear with correlation coefficients more than 0.9990. The linear ranges were 31.44–1572 μg/mL, 25.48–1274 μg/mL, 40.56–2028 μg/mL, 31.44–1572 μg/mL and 26.88–1396 μg/mL for Art, Art B, Art C, DHAA and AA, respectively. The precision ranged from 0.08% to 2.88%, the stability was from 0.96% to 1.66%, and the repeatability was all within 2.42% and had a mean extraction recovery of 96.5% to 100.6%. Conclusion: The established UPLC–PDA method would be valuable for improving the quantitative analysis of sesquiterpene components in Artemisia annua L.

Biosynthesis of artemisinic acid in Artemisia annua

1990 ◽  
Vol 29 (7) ◽  
pp. 2129-2132 ◽  
Author(s):  
Anand Akhila ◽  
Kumkum Rani ◽  
Raghunath S. Thakur
Keyword(s):  
Artemisia Annua ◽  
Artemisinic Acid
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