The main goal of this research is to investigate how learners with different cultural background differ in their interaction style and visual behavior in multimedia-enhanced education, more specifically between groups from the African vs. Asian regions. The researchers conducted a controlled eye-tracking experiment to explore and evaluate the visual behavior of African, Afghan, Japanese and Chinese learners when scanning through different online multimedia contents. The analysis of their eye-gaze patterns and heat-maps revealed significant differences in terms of learners' interaction style, gender, color, text or multimedia preferences. This cross-cultural investigation collectively contributes towards effective use of multimedia technologies in education that ultimately increases learners' engagement and retention.