In-situ data collection at the photon factory macromolecular crystallography beamlines

2016 ◽  
Author(s):  
Yusuke Yamada ◽  
Masahiko Hiraki ◽  
Naohiro Matsugaki ◽  
Ryuichi Kato ◽  
Toshiya Senda
Keyword(s):  
Data Collection ◽  
Macromolecular Crystallography ◽  
Photon Factory ◽  
In Situ Data

scholarly journals Chemical sensors for in situ data collection in the cryosphere

2016 ◽  
Vol 82 ◽  
pp. 348-357 ◽  
Author(s):  
Elizabeth A. Bagshaw ◽  
Alexander Beaton ◽  
Jemma L. Wadham ◽  
Matt Mowlem ◽  
Jon R. Hawkings ◽  
...  
Keyword(s):  
Data Collection ◽  
Chemical Sensors ◽  
In Situ Data

scholarly journals Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography

IUCrJ ◽  
2020 ◽  
Vol 7 (6) ◽  
pp. 1131-1141
Author(s):  
Isabelle Martiel ◽  
Chia-Ying Huang ◽  
Pablo Villanueva-Perez ◽  
Ezequiel Panepucci ◽  
Shibom Basu ◽  
...  
Keyword(s):  
Data Collection ◽  
Phase Contrast ◽  
Low Dose ◽  
Phase Contrast Imaging ◽  
Cubic Phase ◽  
Macromolecular Crystallography ◽  
Contrast Imaging ◽  
X Ray ◽  
Serial Data

Serial protein crystallography has emerged as a powerful method of data collection on small crystals from challenging targets, such as membrane proteins. Multiple microcrystals need to be located on large and often flat mounts while exposing them to an X-ray dose that is as low as possible. A crystal-prelocation method is demonstrated here using low-dose 2D full-field propagation-based X-ray phase-contrast imaging at the X-ray imaging beamline TOMCAT at the Swiss Light Source (SLS). This imaging step provides microcrystal coordinates for automated serial data collection at a microfocus macromolecular crystallography beamline on samples with an essentially flat geometry. This prelocation method was applied to microcrystals of a soluble protein and a membrane protein, grown in a commonly used double-sandwich in situ crystallization plate. The inner sandwiches of thin plastic film enclosing the microcrystals in lipid cubic phase were flash cooled and imaged at TOMCAT. Based on the obtained crystal coordinates, both still and rotation wedge serial data were collected automatically at the SLS PXI beamline, yielding in both cases a high indexing rate. This workflow can be easily implemented at many synchrotron facilities using existing equipment, or potentially integrated as an online technique in the next-generation macromolecular crystallography beamline, and thus benefit a number of dose-sensitive challenging protein targets.

scholarly journals Indian moored observatory in the Arctic for long-term in situ data collection

2016 ◽  
Vol 7 (2) ◽  
pp. 55-61 ◽  
Author(s):  
R Venkatesan ◽  
KP Krishnan ◽  
M Arul Muthiah ◽  
B Kesavakumar ◽  
David T Divya ◽  
...  
Keyword(s):  
Data Collection ◽  
The Arctic ◽  
In Situ Data

Novel combined crystallization plate for high-throughput crystal screening and in situ data collection at a crystallography beamline

2021 ◽  
Vol 77 (9) ◽  
pp. 319-327
Author(s):  
Miao Liang ◽  
Li Yu ◽  
Zhijun Wang ◽  
Huan Zhou ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Data Collection ◽  
High Throughput ◽  
Diffraction Data ◽  
Large Scale ◽  
Liquid Drop ◽  
In Situ Data ◽  
Combined Crystallization ◽  
Manual Manipulation ◽  
Large Scale Screening

In situ microplates are small in size, crystal cultivation and operation are difficult, and the efficiency of crystal screening is relatively low. To solve this problem, a novel combined crystallization plate was designed for high-throughput crystal cultivation and in situ data collection. A frame was used to hold 48 in situ microplates, and the in situ microplates were sealed on one side with an ultralow background-scattering Kapton film. An automatic liquid handler (Mosquito) was used to add a liquid drop to the in situ microplates in the frame, and CrystalClear HD tape was used to seal the frame. A sealed frame holding 48 microplates was developed as a novel combined crystallization plate and was used for crystal cultivation under different conditions and in situ data collection at the synchrotron beamline. Moreover, individual microplates can be separated from the combined crystal plate and then fixed on a magnetic base or loaded onto a UniPuck for in situ data collection. Automatic grid scanning was used to locate crystals. The efficiency of the combined crystallization plate for crystal screening was verified. This method avoids the manual manipulation of crystals during crystal screening and diffraction data collection; therefore, the combined crystallization plate is suitable for large-scale screening of microcrystals.

IN-SITU DATA COLLECTION/MONITORING FOR MODELING THE FINAL SETTLING TANK (FSTs) AT WARDS ISLAND WPCP-BATTERY “E”

2007 ◽  
Vol 2007 (2) ◽  
pp. 311-336
Author(s):  
Krish Ramalingam ◽  
John Fillos ◽  
Allen Deur ◽  
Keith Beckmann
Keyword(s):  
Data Collection ◽  
Settling Tank ◽  
In Situ Data

scholarly journals ESRF/DBT/EMBL:BM14-2: MX station for in situ, dehydration and MAD experiments

2014 ◽  
Vol 70 (a1) ◽  
pp. C323-C323
Author(s):  
Babu Manjasetty ◽  
Akim Khadrouche ◽  
Bernard Lavault ◽  
Franck Felissaz ◽  
Trevor Mairs ◽  
...  
Keyword(s):  
Data Collection ◽  
High Energy ◽  
Control Device ◽  
In Situ Data ◽  
Multiple Wavelength ◽  
Concomitant Reduction ◽  
Diffraction Patterns ◽  
To Come ◽  
Novel Devices

BM14 was the first dedicated macromolecular crystallography (MX) beamline to come into operation at the ESRF during 1995. This experimental station has been designed specifically to produce MX diffraction data using Single/Multiple-wavelength Anomalous Diffraction (SAD/MAD) methods (www.bm14.eu). Currently, beamline is operated by a consortium between the ESRF, the EMBL-Grenoble and the Department of Biotechnology (DBT), India. Recently, the beamline optics was fully upgraded (hence the new denomination BM14-2). A four-time increase in beam brilliance is achieved with the concomitant reduction in average exposure time (~5s today versus 20s before), leading to a substantial gain in the screening capacity. Upgrade includes a new channel-cut crystal equipped with a second crystal pusher to reject high-energy harmonics. The latter device turned to be crucial for Sulphur-SAD experiment success rate. The special goniometer head is installed on microdiffractometer (MD2) goniometer which is capable of handling the CrystalDirect (CD) nanocrystallisation plates [1] so that any crystallisation hit can be exposed to the beam. The plate characteristics are optimal for in situ data collection and they produce diffraction patterns with significantly reduced background. A newly designed dehydration-to-cryogenic nozzle-changer device is also installed in order to avoid the down time between the experiments (such as, In situ screening, humidity control device (HC1), standard cryogenic data collection). European users may apply for access via the Biostruct-X program (www.biostruct-x.eu) both for CD plate nanocrystallisation trials and for beamtime. Users from India are encouraged to apply from DBT-Portal (http://process.mbu.iisc.ernet.in/BM14/index.jsp) for beamtime. The optics hutch refurbishment and implementation of the novel devices in the experimental hutch opens promising perspectives for collecting data at room temperature and that are of importance to the MX users.

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