Novel combined crystallization plate for high-throughput crystal screening and in situ data collection at a crystallography beamline

2021 ◽  
Vol 77 (9) ◽  
pp. 319-327
Author(s):  
Miao Liang ◽  
Li Yu ◽  
Zhijun Wang ◽  
Huan Zhou ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Data Collection ◽  
High Throughput ◽  
Diffraction Data ◽  
Large Scale ◽  
Liquid Drop ◽  
In Situ Data ◽  
Combined Crystallization ◽  
Manual Manipulation ◽  
Large Scale Screening

In situ microplates are small in size, crystal cultivation and operation are difficult, and the efficiency of crystal screening is relatively low. To solve this problem, a novel combined crystallization plate was designed for high-throughput crystal cultivation and in situ data collection. A frame was used to hold 48 in situ microplates, and the in situ microplates were sealed on one side with an ultralow background-scattering Kapton film. An automatic liquid handler (Mosquito) was used to add a liquid drop to the in situ microplates in the frame, and CrystalClear HD tape was used to seal the frame. A sealed frame holding 48 microplates was developed as a novel combined crystallization plate and was used for crystal cultivation under different conditions and in situ data collection at the synchrotron beamline. Moreover, individual microplates can be separated from the combined crystal plate and then fixed on a magnetic base or loaded onto a UniPuck for in situ data collection. Automatic grid scanning was used to locate crystals. The efficiency of the combined crystallization plate for crystal screening was verified. This method avoids the manual manipulation of crystals during crystal screening and diffraction data collection; therefore, the combined crystallization plate is suitable for large-scale screening of microcrystals.

Meet your Users in Situ Data Collection from within Apps in Large-Scale Deployments

2015 ◽  
Vol 6 (3) ◽  
pp. 17-32 ◽  
Author(s):  
Nikolaos Batalas ◽  
Javier Quevedo-Fernandez ◽  
Jean-Bernard Martens ◽  
Panos Markopoulos
Keyword(s):  
Data Collection ◽  
Large Scale ◽  
Creative Design ◽  
Research Perspective ◽  
Software Application ◽  
In Situ Data ◽  
Research Perspectives ◽  
In The Wild ◽  
Design Representations

Increasingly, ‘app-store' releases of software are used as a vehicle for large-scale user trials ‘in the wild'. Several opportunities and methodological challenges arise from having little or no access to users, other than through the application itself. So far, researchers have needed to hardcode survey items into the software application studied, which is laborious and error prone. This paper discusses how these problems are addressed using TEMPEST, a platform for longitudinal in situ data collection. The authors illustrate the use of TEMPEST to study the deployment and real-world use of a tablet application called idAnimate; this application has been designed to support the creation of simple animations as design representations during the creative design process. The authors discuss how the tool has supported the gathering of data in over 4000 installations, both from a development and research perspective, and relate their experiences to current research perspectives on large-scale app trials.

scholarly journals Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline

Crystals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 798 ◽  
Author(s):  
Miao Liang ◽  
Zhijun Wang ◽  
Hai Wu ◽  
Li Yu ◽  
Bo Sun ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Data Collection ◽  
Diffraction Data ◽  
The Novel ◽  
X Ray Diffraction ◽  
Shanghai Synchrotron Radiation Facility ◽  
In Situ Data ◽  
Crystallization Experiments ◽  
Efficient Data

An efficient data collection method is important for microcrystals, because microcrystals are sensitive to radiation damage. Moreover, microcrystals are difficult to harvest and locate owing to refraction effects from the surface of the liquid drop or optically invisible, owing to their small size. Collecting X-ray diffraction data directly from the crystallization devices to completely eliminate the crystal harvesting step is of particular interest. To address these needs, novel microplates combining crystal growth and data collection have been designed for efficient in situ data collection and fully tested at Shanghai Synchrotron Radiation Facility (SSRF) crystallography beamlines. The design of the novel microplates fully adapts the advantage of in situ technology. Thin Kapton membranes were selected to seal the microplate for crystal growth, the crystallization plates can support hanging drop and setting drop vapor diffusion crystallization experiments. Then, the microplate was fixed on a magnetic base and mounted on the goniometer head for in situ data collection. Automatic grid scanning was applied for crystal location with a Blu-Ice data collection system and then in situ data collection was performed. The microcrystals of lysozyme were selected as the testing samples for diffraction data collection using the novel microplates. The results show that this method can achieve comparable data quality to that of the traditional method using the nylon loop. In addition, our method can efficiently and diversely perform data acquisition experiments, and be especially suitable for solving structures of multiple crystals at room temperature or cryogenic temperature.

High-throughput counter-diffusion capillary crystallization andin situdiffraction using high-pressure freezing in protein crystallography

2012 ◽  
Vol 45 (5) ◽  
pp. 999-1008 ◽  
Author(s):  
Mareike Kurz ◽  
Beat Blattmann ◽  
Andres Kaech ◽  
Christophe Briand ◽  
Paul Reardon ◽  
...  
Keyword(s):  
High Pressure ◽  
Low Temperature ◽  
Data Collection ◽  
High Throughput ◽  
Diffraction Data ◽  
High Throughput Screening ◽  
Structural Information ◽  
Major Step ◽  
Counter Diffusion

Post-crystallization treatments such as manual fishing of crystals and soaking in cryoprotectant solutions, especially of large macromolecular complexes and membrane proteins, are cumbersome and often lead to crystal damage and reduced diffraction data quality. Here, a capillary crystallization plate is presented that simultaneously allows counter-diffusion crystallization at the nanolitre scale in a high-throughput screening mode, low-temperaturein situdiffraction data collection from crystals after cryoprotection and low-temperaturein situdata collection of crystals without the addition of any cryoprotectant after high-pressure (HP) freezing. The development of this plate and plunge cooling of crystals in the capillaries is a major step towards implementing automatedin situhigh-throughput crystal diffraction data collection at a synchrotron beamline. In combination with HP freezing this offers a new opportunity to obtain structural information from fragile crystals of supramolecular complexes that might otherwise not be feasible.

scholarly journals Basic Mechanical Parameter Relations of Soft Clay in Pearl River Delta

2014 ◽  
Vol 8 (1) ◽  
pp. 320-325 ◽  
Author(s):  
Zhangming Li ◽  
Na Qi ◽  
Zhibin Masumi ◽  
Weidi Lin
Keyword(s):  
Shear Strength ◽  
Pearl River Delta ◽  
Large Scale ◽  
Soft Clay ◽  
River Delta ◽  
Pearl River ◽  
Cylindrical Cavity Expansion ◽  
In Situ Data ◽  
The Pearl River

Basic parameters relations among CPT parameters, un-drained strength and other mechanical parameters of soft clay are presented based on an elastic-plastic solution for cylindrical cavity expansion for soil investigation in energy engineering. The relation between CPT parameters and shear strength from vane test is also presented based on the result. Thus, the CPT parameters can be determined directly by elastic parameters and shear strength or vane shear parameters and vice versa. That makes it possible to save the high test costs and provide theoretical formulas to avoid some tests which are limited due to the site and/or other condition. Results are compared between the relations and in situ data at a large-scale project in the Pearl River Delta. The results showed consistency between the relation and in situ data.

In-situ data collection at the photon factory macromolecular crystallography beamlines

2016 ◽  
Author(s):  
Yusuke Yamada ◽  
Masahiko Hiraki ◽  
Naohiro Matsugaki ◽  
Ryuichi Kato ◽  
Toshiya Senda
Keyword(s):  
Data Collection ◽  
Macromolecular Crystallography ◽  
Photon Factory ◽  
In Situ Data

scholarly journals Freeboard, snow depth and sea-ice roughness in East Antarctica from in situ and multiple satellite data

2011 ◽  
Vol 52 (57) ◽  
pp. 242-248 ◽  
Author(s):  
Thorsten Markus ◽  
Robert Massom ◽  
Anthony Worby ◽  
Victoria Lytle ◽  
Nathan Kurtz ◽  
...  
Keyword(s):  
Sea Ice ◽  
Correlation Coefficient ◽  
Satellite Data ◽  
Snow Depth ◽  
Large Scale ◽  
Mean Difference ◽  
Laser Altimeter ◽  
In Situ Data ◽  
Ice Roughness

AbstractIn October 2003 a campaign on board the Australian icebreaker Aurora Australis had the objective to validate standard Aqua Advanced Microwave Scanning Radiometer (AMSR-E) sea-ice products. Additionally, the satellite laser altimeter on the Ice, Cloud and land Elevation Satellite (ICESat) was in operation. To capture the large-scale information on the sea-ice conditions necessary for satellite validation, the measurement strategy was to obtain large-scale sea-ice statistics using extensive sea-ice measurements in a Lagrangian approach. A drifting buoy array, spanning initially 50 km × 100 km, was surveyed during the campaign. In situ measurements consisted of 12 transects, 50–500 m, with detailed snow and ice measurements as well as random snow depth sampling of floes within the buoy array using helicopters. In order to increase the amount of coincident in situ and satellite data an approach has been developed to extrapolate measurements in time and in space. Assuming no change in snow depth and freeboard occurred during the period of the campaign on the floes surveyed, we use buoy ice-drift information as well as daily estimates of thin-ice fraction and rough-ice vs smooth-ice fractions from AMSR-E and QuikSCAT, respectively, to estimate kilometer-scale snow depth and freeboard for other days. the results show that ICESat freeboard estimates have a mean difference of 1.8 cm when compared with the in situ data and a correlation coefficient of 0.6. Furthermore, incorporating ICESat roughness information into the AMSR-E snow depth algorithm significantly improves snow depth retrievals. Snow depth retrievals using a combination of AMSR-E and ICESat data agree with in situ data with a mean difference of 2.3 cm and a correlation coefficient of 0.84 with a negligible bias.

scholarly journals Chemical sensors for in situ data collection in the cryosphere

2016 ◽  
Vol 82 ◽  
pp. 348-357 ◽  
Author(s):  
Elizabeth A. Bagshaw ◽  
Alexander Beaton ◽  
Jemma L. Wadham ◽  
Matt Mowlem ◽  
Jon R. Hawkings ◽  
...  
Keyword(s):  
Data Collection ◽  
Chemical Sensors ◽  
In Situ Data

scholarly journals MicroED data collection with SerialEM

2018 ◽  
Author(s):  
M. Jason de la Cruz ◽  
Michael W. Martynowycz ◽  
Johan Hattne ◽  
Tamir Gonen
Keyword(s):  
Data Collection ◽  
High Throughput ◽  
Large Scale ◽  
Large Scale Data ◽  
Data Analyses ◽  
Continuous Rotation ◽  
Scale Data

AbstractWe developed a procedure for the cryoEM method MicroED using SerialEM. With this approach, SerialEM coordinates stage rotation, microscope operation, and camera functions for automated continuous-rotation MicroED data collection. More than 300 datasets can be collected overnight in this way, facilitating high-throughput MicroED data collection for large-scale data analyses.

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