Development of a concentration unit for the automated system for incorporating L-asparaginase into human erythrocytes

2019 ◽  
Author(s):  
A. S. Makagonova ◽  
N. S. Kushnir ◽  
D. V. Borsakova ◽  
A. V. Samorodov ◽  
F. I. Ataullakhanov
Author(s):  
S. A. Livesey ◽  
A. A. del Campo ◽  
E. S. Griffey ◽  
D. Ohlmer ◽  
T. Schifani ◽  
...  

The aim of this study is to compare methods of sample preparation for elemental analysis. The model system which is used is the human erythrocyte. Energy dispersive spectroscopic analysis has been previously reported for cryofixed and cryosectioned erythrocytes. Such work represents the reference point for this study. The use of plastic embedded samples for elemental analysis has also been documented. The work which is presented here is based on human erythrocytes which have been either chemically fixed and embedded or cryofixed and subsequently processed by a variety of techniques which culminated in plastic embedded samples.Heparinized and washed erythrocytes were prepared by the following methods for this study :(1). Chemical fixation in 4% paraformaldehyde/0.25% glutaraldehyde/0.2 M sucrose in 0.1 M Na cacodylate, pH 7.3 for 30 min, followed by ethanol dehydration, infiltration and embedding in Lowicryl K4M at -20° C.


1974 ◽  
Author(s):  
Peter H. Henry ◽  
Roy A. Turner ◽  
Robert B. Matthie

2020 ◽  
Vol 6 (2) ◽  
pp. 147-153
Author(s):  
Muhamad Yusup ◽  
Po. Abas Sunarya ◽  
Krisandi Aprilyanto

System The process of counting and storing in a manual water reservoir analysis has a high percentage of error rate compared to an automated system. In a company industry, especially in the WWT (Waste Water Treatment) section, it has several reservoir tanks as stock which are still counted manually. The ultrasonic sensor is placed at the top of the WWT tank in a hanging position. Basically, to measure the volume in a tank only variable height is always changing. So by utilizing the function of the ultrasonic sensor and also the tube volume formula, the stored AIR volume can be monitored in real time based on IoT using the Blynk application. From the sensor, height data is obtained which then the formula is processed by Arduino Wemos and then information is sent to the MySQL database server via the WIFI network.


In automated control systems for technical processes, the conversion of a continuous signal into a digital code and vice versa from a digital code to a continuous (analog) value is widely used. For direct type converters often used the term ADC, the reverse - DAC. The characteristics of the converters often dramatically affect the parameters of the entire automated system. The importance of the correct choice of ADCs and DACs has especially increased recently in connection with the mass introduction of microcontrollers MC. Indeed, in addition to the ADC and DAC, it is necessary to place the processor core in the microcontroller's crystal, I/O interfaces and many other elements necessary for the functioning of the MC. The use of information converters in the construction industry imposes additional requirements on converters: for example, in building monitoring systems, precision ADCs with extremely high accuracy are often required (while performance may be low), in other applications it is necessary to provide the necessary parameters at a high level of industrial interference, etc. This article explores issues related to the rational choice of ADCs and DACs, taking into account current trends in the IT field and the specifics of work in the construction industry. Sigma-Delta converters are noted as the most promising models of direct type converters.


1960 ◽  
Vol XXXIV (II) ◽  
pp. 305-311 ◽  
Author(s):  
M. G. Woldring ◽  
A. Bakker ◽  
H. Doorenbos

ABSTRACT The red cell triiodothyronine uptake technique as used in our hospital is described. Incubation time is of almost no importance. The temperature during incubation should be 37° C. Further improvement of the technique is obtained when all blood samples are brought up to 40 % haematocrit prior to incubation. Clinical results are discussed. It is yet too early to give a definite assessment of its clinical value, but it is definitely superior to the measurement of the BMR.


Author(s):  
Jacqueline Tran ◽  
Dianne Bautista ◽  
Zoltan Seres ◽  
Loic Cornaut ◽  
Torquil Gundlach ◽  
...  

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