scholarly journals Studies on lolium multiflorum endosperm in tissue culture I. Nutrition

1973 ◽  
Vol 26 (1) ◽  
pp. 123 ◽  
Author(s):  
M MeryI Smith ◽  
BA Stone
Keyword(s):  
Tissue Culture ◽  
Suspension Culture ◽  
Doubling Time ◽  
Lolium Multiflorum ◽  
Carbon Sources ◽  
Equimolar Mixture ◽  
Liquid Suspension ◽  
The Mean ◽  
Liquid Suspension Culture

Stocks of L. multiflorum endosperm callus have been maintained in liquid suspension culture on a modified White's medium for 5 years. The mean doubling time under the conditions used is 3� 2 days. Best growth is obtained on sucrose; fructose and glucose are good carbon sources, whereas growth is only moderate on an equimolar mixture of both.

scholarly journals TEMPERATURE-SENSITIVE VARIANTS OF NICOTIANA TABACUM ISOLATED FROM SOMATIC CELL CULTURE

Genetics ◽  
1979 ◽  
Vol 92 (1) ◽  
pp. 215-221
Author(s):  
Russell L Malmberg
Keyword(s):  
Nicotiana Tabacum ◽  
Suspension Culture ◽  
Selection Procedure ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Permissive Temperature ◽  
Temperature Growth ◽  
Liquid Suspension ◽  
Slow Growing ◽  
Liquid Suspension Culture

ABSTRACT Temperature-sensitive variants of Nicotiana tabacum were isolated from a liquid suspension culture of somatic cells by a negative selection procedure, using bromodeoxyuridine and light. A total of nine such variants have been recovered, with an estimated rate of 2 × 10-7 per cell division. The appearance of the variants at the permissive temperature varied from nearly wild type, white and friable, to brown, compact and slow growing. Two of the variants adapted from growth on solid medium to growth in a liquid suspension culture; these were further characterized for chromosome number, growth rate, cell death rate at the restrictive temperature, growth on nutritionally modified media, and RNA and protein synthesis. The variants have been placed on regeneration media, and one of them has produced plantlets. Leaves from a plantlet have been placed on callus-inducing media, and the resulting callus displayed the temperature-sensitive phenotype.

Effect of adroxazine, a heterocyclic compound of the adrenals, on the rate of replication of lymphoblastoid cells

1985 ◽  
Vol 63 (2) ◽  
pp. 122-127 ◽  
Author(s):  
F. A. H. Rice ◽  
J. D. McCurdy ◽  
C. Oresajo
Keyword(s):  
Tissue Culture ◽  
Dna Synthesis ◽  
Heterocyclic Compound ◽  
Doubling Time ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Lymphoblastoid Cells ◽  
Resting Time ◽  
The Mean

It has been found that the addition of 10−2–10−8 μL/mL of adroxazine, a heterocyclic compound of the adrenals, to the tissue culture medium increases the rate at which three strains of lymphoblastoid cells replicate. For example, the addition of 10−5 μg/mL of adroxazine to cultured L5178Y cells decreases their doubling time from approximately 11 to 8 h and addition of 10−3 μg/mL of adroxazine to the tissue culture medium of Huly-16 or Huly-29 cells decreases their doubling time from approximately 43 to 34 and 36 h, respectively. Other concentrations of adroxazine, from 10−2 to 10−8 μg/mL, cause a less but measurable decrease in doubling time. The effect of adroxazine on the rate of incorporation of [3H]thymidine into acid-precipitable material by lymphoblastoid cells was found to parallel the effect of adroxazine on doubling time. The effect of adroxazine on the length of DNA synthesis and mitosis was calculated from the percent of cells that are labeled with [3H]thymidine during a 20-min interval and the percent of cells in mitosis, respectively. Results showed that adroxazine does not decrease the length of DNA synthesis or mitosis, but markedly decreases the sum of pre- and post-mitotic resting times [Formula: see text]. Addition of 10−3 μg/mL of adroxazine to the medium decreases the resting times of Huly-16 cells from approximately 20 to 10 h and Huly-29 cells from approximately 12 to 5 h. Addition of 10−5 μg/mL of adroxazine to the medium decreases the resting time of L5178Y cells from 3.3 to 0.1 h. The effect of adroxazine on [Formula: see text] and [Formula: see text] was calculated by using the fraction of labeled cells in mitosis method. It was found that adroxazine markedly decreases the mean duration of both [Formula: see text] and [Formula: see text].

All-Trans Retinoic Acid Delays the Differentiation of Primitive Hematopoietic Precursors (lin−c-kit+Sca-1+) While Enhancing the Terminal Maturation of Committed Granulocyte/Monocyte Progenitors

Blood ◽  
1999 ◽  
Vol 94 (2) ◽  
pp. 483-495 ◽  
Author(s):  
Louise E. Purton ◽  
Irwin D. Bernstein ◽  
Steven J. Collins
Keyword(s):  
Retinoic Acid ◽  
Suspension Culture ◽  
Terminal Differentiation ◽  
Potent Inducer ◽  
Cell Production ◽  
Trans Retinoic Acid ◽  
Liquid Suspension ◽  
All Trans Retinoic Acid ◽  
Colony Forming Cell ◽  
Liquid Suspension Culture

All-trans retinoic acid (ATRA) is a potent inducer of terminal differentiation of malignant promyelocytes, but its effects on more primitive hematopoietic progenitors and stem cells are less clear. In this study, we investigated the effect of ATRA on highly enriched murine hematopoietic precursor cells (lin−c-kit+Sca-1+) grown in liquid suspension culture for 28 days. ATRA initially slowed the growth of these hematopoietic precursors but prolonged and markedly enhanced their colony-forming cell production compared with the hematopoietic precursors cultured in its absence. At 7 and 14 days of culture, a substantially greater percentage of cells cultured with ATRA did not express lineage-associated antigens (55.4% at day 7 and 68.6% at day 14) and retained expression of Sca-1 (44.7% at day 7 and 79.9% at day 14) compared with cells grown in its absence (lin−cells: 31.5% at day 7 and 4% at day 14; Sca-1+: 10.4% at day 7 and 0.7% at day 14). Moreover, a marked inhibition of granulocyte production was observed in cultures continuously incubated with ATRA. Significantly, ATRA markedly prolonged and enhanced the production of transplantable colony-forming unit-spleen (CFU-S) during 14 days of liquid suspension culture. In contrast with its effects on primitive lin−c-kit+Sca-1+hematopoietic precursors, ATRA did not exert the same effects on the more committed lin−c-kit+Sca-1−progenitor cells. Moreover, the late addition of ATRA (7 days post-culture initiation) to cultures of primitive hematopoietic precursors resulted in a marked decrease in colony-forming cell production in these cultures, which was associated with enhanced granulocyte differentiation. These observations indicate that ATRA has different effects on hematopoietic cells depending on their maturational state, preventing and/or delaying the differentiation of primitive hematopoietic precursors while enhancing the terminal differentiation of committed granulocyte/monocyte progenitors.

scholarly journals Studies on lolium multiflorum endosperm in tissue culture II. Fine structure of cells and cell walls and the development of cell walls

1973 ◽  
Vol 26 (1) ◽  
pp. 135 ◽  
Author(s):  
DJ Mares ◽  
BA Stone
Keyword(s):  
Tissue Culture ◽  
Fine Structure ◽  
Cell Walls ◽  
Lolium Multiflorum ◽  
Suspension Cultures ◽  
Protein Bodies ◽  
Starch Granules ◽  
Cell Organelles ◽  
Stages Of Growth ◽  
Liquid Suspension

Log phase cells of L. multiflorum endosperm grown in liquid suspension cultures contain large nuclei, mitochondria, protein bodies, compound starch granules, small vacuoles, and multilayered membranous structures. At later stages of growth the cell organelles, protein bodies, and starch granules disappear and in senescent cultures many empty cells are seen.

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