scholarly journals Studies on lolium multiflorum endosperm in tissue culture II. Fine structure of cells and cell walls and the development of cell walls

1973 ◽  
Vol 26 (1) ◽  
pp. 135 ◽  
Author(s):  
DJ Mares ◽  
BA Stone
Keyword(s):  
Tissue Culture ◽  
Fine Structure ◽  
Cell Walls ◽  
Lolium Multiflorum ◽  
Suspension Cultures ◽  
Protein Bodies ◽  
Starch Granules ◽  
Cell Organelles ◽  
Stages Of Growth ◽  
Liquid Suspension

Log phase cells of L. multiflorum endosperm grown in liquid suspension cultures contain large nuclei, mitochondria, protein bodies, compound starch granules, small vacuoles, and multilayered membranous structures. At later stages of growth the cell organelles, protein bodies, and starch granules disappear and in senescent cultures many empty cells are seen.

scholarly journals Studies on lolium multiflorum endosperm in tissue culture I. Nutrition

1973 ◽  
Vol 26 (1) ◽  
pp. 123 ◽  
Author(s):  
M MeryI Smith ◽  
BA Stone
Keyword(s):  
Tissue Culture ◽  
Suspension Culture ◽  
Doubling Time ◽  
Lolium Multiflorum ◽  
Carbon Sources ◽  
Equimolar Mixture ◽  
Liquid Suspension ◽  
The Mean ◽  
Liquid Suspension Culture

Stocks of L. multiflorum endosperm callus have been maintained in liquid suspension culture on a modified White's medium for 5 years. The mean doubling time under the conditions used is 3� 2 days. Best growth is obtained on sucrose; fructose and glucose are good carbon sources, whereas growth is only moderate on an equimolar mixture of both.

Studies on Lolium Multiflorum Endosperm in Tissue Culture III. Structural Studies on the Cell Walls

1978 ◽  
Vol 31 (5) ◽  
pp. 573 ◽  
Author(s):  
Robin L Anderson ◽  
BA Stone
Keyword(s):  
Tissue Culture ◽  
Cell Wall ◽  
Protein Content ◽  
Cell Walls ◽  
Lolium Multiflorum ◽  
Structural Studies ◽  
Methylation Analysis ◽  
Successive Extraction

Cell wall preparations from ryegrass endosperm cells grown in tissue culture contain polysaccharides composed of glucose, xylose, arabinose and galactose residues. The protein content is very low. The cell wa.ll preparations have been fractionated by successive extraction with water at 40�C, 8 M urea, O�5 M KOH, 4�27 M KOH and 6 M NaOH-O' 81 M H3B03 ? The polysaccharides in the fractions were identified by monosaccharide and methylation analysis, supplemented by the use of a specific fi-glucan hydrolase. The results indicate that the three' main types of polysaccharide present are 1,3: 1,4-fi-glucan, arabinoxylan and/or galacto-arabinoxylan and cellulose.

Cell Fine Structure as Revealed in Dessicator-Dried Resinless Sections

1981 ◽  
Vol 39 ◽  
pp. 622-623
Author(s):  
R. P. Becker ◽  
J. J. Wolosewick ◽  
J. Ross-Stanton
Keyword(s):  
Fine Structure ◽  
Tannic Acid ◽  
Three Dimensional ◽  
Albino Rats ◽  
Cell Organelles ◽  
Vascular Perfusion ◽  
Thin Sections ◽  
Carbon Coated ◽  
Embedding Medium ◽  
Polyethylene Glycol 6000

Methodology has been introduced recently which allows transmission and scanning electron microscopy of cell fine structure in semi-thin sections unencumbered by an embedding medium. Images obtained from these “resinless” sections show a three-dimensional lattice of microtrabeculfee contiguous with cytoskeletal structures and membrane-bounded cell organelles. Visualization of these structures, especially of the matiiDra-nous components, can be facilitated by employing tannic acid in the fixation step and dessicator drying, as reported here.Albino rats were fixed by vascular perfusion with 2% glutaraldehyde or 1.5% depolymerized paraformaldehyde plus 2.5% glutaraldehyde in 0.1M sodium cacodylate (pH 7.4). Tissues were removed and minced in the fixative and stored overnight in fixative containing 4% tannic acid. The tissues were rinsed in buffer (0.2M cacodylate), exposed to 1% buffered osmium tetroxide, dehydrated in ethyl alcohol, and embedded in pure polyethylene glycol-6000 (PEG). Sections were cut on glass knives with a Sorvall MT-1 microtome and mounted onto poly-L-lysine, formvar-carbon coated grids while submerged in a solution of 95% ethanol containing 5% PEG.

UDP-D-glucose 4-epimerase activity of the tissue culture of white poplars (Populus alba L. var. pyramidalis)

1982 ◽  
Vol 47 (5) ◽  
pp. 1530-1536 ◽  
Author(s):  
Ladislav Bilisics ◽  
Štefan Karácsonyi ◽  
Marta Kubačková
Keyword(s):  
Tissue Culture ◽  
Cell Walls ◽  
Enzyme Preparation ◽  
Uridine Diphosphate ◽  
Populus Alba ◽  
Activity Change ◽  
White Poplar ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Galactose Content

The presence of UDP-D-glucose 4-epimerase (EC 5.1.3.2) in the culture tissue of white poplar was evidenced. As found, the partially purified enzyme preparation contained UDP-D-glucose glucosyltransferase, UDP-D-galactose galactosyltransferase and non-specific enzymes able to cleave the uridine-diphosphate saccharides into the appropriate hexose monophosphates. The activity change of UDP-D-glucose 4-epimerase in tissue culture cells during the growth was in accord with changes in D-galactose content in cell walls and indicated the possibility to regulate the formation of polysaccharides containing D-galactose at the level of production of UDP-D-galactose in cells.

The Fine Structure of Fossil Plant Cell Walls

Science ◽  
1984 ◽  
Vol 225 (4662) ◽  
pp. 621-623 ◽  
Author(s):  
E. L. SMOOT ◽  
T. N. TAYLOR
Keyword(s):  
Fine Structure ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Fossil Plant

scholarly journals Characterization of the hydroxyproline-rich protein core of an arabinogalactan-protein secreted from suspension-cultured Lolium multiflorum (Italian ryegrass) endosperm cells

1989 ◽  
Vol 264 (3) ◽  
pp. 857-862 ◽  
Author(s):  
P A Gleeson ◽  
M McNamara ◽  
R E H Wettenhall ◽  
B A Stone ◽  
G B Fincher
Keyword(s):  
Polyclonal Antibodies ◽  
Lolium Multiflorum ◽  
Italian Ryegrass ◽  
Arabinogalactan Protein ◽  
Myeloma Protein ◽  
Protein Core ◽  
Liquid Suspension ◽  
Peptide Component ◽  
Immunodominant Epitopes

An arabinogalactan-protein (AGP) purified from the filtrate of liquid-suspension-cultured Italian-ryegrass (Lolium multiflorum) endosperm cells by affinity chromatography on myeloma protein J539-Sepharose was deglycosylated with trifluoromethanesulphonic acid to remove polysaccharide chains that are covalently associated with hydroxyproline residues in the peptide component of the proteoglycan. The protein core, which accounts for less than 10% (w/w) of the intact proteoglycan, was purified by h.p.l.c. It has an apparent Mr of 35,000, but reacts very poorly with both Coomassie Brilliant Blue R and silver stains. Amino-acid-sequence analysis of the N-terminus of the h.p.l.c.-purified protein core and of tryptic peptides generated from the unpurified protein reveals a high content of hydroxyproline and alanine. These are sometimes arranged in short (Ala-Hyp) repeat sequences of up to six residues. Polyclonal antibodies raised against the protein core do not cross-react with native AGP, the synthetic peptide (Ala-Hyp)4, poly-L-hydroxyproline or poly-L-proline. The results suggest that the polysaccharide chains in the native AGP render the protein core of the proteoglycan inaccessible to the antibodies and that the immunodominant epitopes include domains of the protein other than those rich in Ala-Hyp repeating units.

Fine structure of the arabinogalactan-protein from Lolium multiflorum

1987 ◽  
Vol 162 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Antony Bacic ◽  
Shirley C. Churms ◽  
Alistair M. Stephen ◽  
Peter B. Cohen ◽  
Geoffrey B. Fincher
Keyword(s):  
Fine Structure ◽  
Lolium Multiflorum ◽  
Arabinogalactan Protein

Preservation of cyanobacteria Glaucospira laxissima at low temperature

2020 ◽  
pp. 50-53
Author(s):  
Daria I. Petrukhina
Keyword(s):  
Low Temperature ◽  
Suspension Cultures ◽  
Short Paper ◽  
Dmso Solution ◽  
Liquid Suspension

This short paper addresses viability and renewed growth of cyanobacteria Glaucospira laxissima after 1-year preservation under -80°C in a deep freezer. To storage in liquid suspension cultures with min 40% viability were used 10%-glucose and DMSO solution.

Effects of pretreatments with sulfhydryls and alkylating agents on spheroplast formation from Schwanniomyces species

1984 ◽  
Vol 30 (3) ◽  
pp. 368-374 ◽  
Author(s):  
T. M. Dowhanick ◽  
C. J. Panchal ◽  
G. G. Stewart
Keyword(s):  
Cell Wall ◽  
Stationary Phase ◽  
Growth Phase ◽  
Cell Walls ◽  
Alkylating Agents ◽  
Inhibitory Effects ◽  
Late Stationary Phase ◽  
Stages Of Growth ◽  
Degradative Enzymes ◽  
Sulfhydryl Compounds

Pretreatment of cells with β-mercaptoethanol, dithiothreitol, or cysteine increased the rate of spheroplast formation at all stages of growth in Schwanniomyces castellii and S. occidentalis, but had little effect on final yields of spheroplasts when compared with controls. Pretreatment with iodoacetate and cystine resulted in decreased rates of formation and lower yields of spheroplasts at all stages of growth for both species. The enhanced rates of spheroplast formation in the presence of the sulfhydryl compounds were more pronounced when the experimental cells were derived from early or late stationary phase cultures, whereas the inhibitory effects of cystine and iodoacetate were not associated with the growth phase. In agreement with extant data on other yeast genera, sulfhydryl compounds increased the susceptibility of the yeast cell wall to degradative enzymes, whilst alkylating agents decreased susceptibility of cell walls to these enzymes.

Sign in / Sign up

Export Citation Format

Share Document