Characterization of a Temperature-Sensitive Female Sterile Mutant (l(1)1074TS) in Drosophila Melanogaster

A new X-linked temperature-sensitive female sterile mutant (/(1)1074") is described. The nonpermissive temperature for this mutant is 29�C. There are two temperature-sensitive periods during development-one between the 6th and 12th hours of embryogenesis and a second commencing during the first larval instar and terminating at mid pupation. Embryological abnormalities first become apparent during gastrulation and eventually these result in the breakdown of organogenesis and the complete absence of normal muscular contractions. Preconditioning mutant females at the nonpermissive temperature for up to 48 h enhances the abnormal embryological effects produced by the mutant.

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THE INTERACTION OF TWO COMPLEX LOCI, ZESTE AND BITHORAX IN DROSOPHILA MELANOGASTER

Genetics ◽

10.1093/genetics/75.2.299 ◽

1973 ◽

Vol 75 (2) ◽

pp. 299-321

Author(s):

T C Kaufman ◽

S E Tasaka ◽

D T Suzuki

Keyword(s):

Drosophila Melanogaster ◽

Heterocyclic Compounds ◽

Larval Instar ◽

Sensitive Period ◽

Temperature Sensitive ◽

Extreme Form ◽

The Third ◽

Specific Allele ◽

Pigment Change

ABSTRACT It has been found that certain alleles of the zeste locus (za 1-1.0) have no phenotype of their own, but interact with certain alleles at the bithorax locus (bx 3-58.8). This interaction takes the form of an enhancement of the homeotic bx phenotype to a more extreme form—i.e., the metathorax is transformed into mesothorax in varying degrees depending on the bx allele used. This enhancement is somewhat reminiscent of the transvection effect described by Lewis (1954). The characterization of the interaction thus far has shown that the enhancement only effects bx alleles which arise spontaneously, whereas the origin of the za allele is unimportant. The gene claret nondisjunctional was used for the production of gynandromorphs which showed that the enhancing ability of za, like the eye pigment change caused by z, is autonomous. The enhancement of one specific allele (bx34e), which is temperature-sensitive, has allowed a delineation of the temperature-sensitive period of the bithorax locus to a period extending from the middle of the second larval instar to the middle of the third larval instar. These results, as well as those of other enhancer and suppressor systems in Drosophila, have revealed the possibility of the involvement of heterocyclic compounds in the control of cell determination and fate in Drosophila melanogaster.

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Characterization of two temperature-sensitive mutants of type 5 adenovirus with mutations in the 100,000-dalton protein gene.

Journal of Virology ◽

10.1128/jvi.40.2.491-500.1981 ◽

1981 ◽

Vol 40 (2) ◽

pp. 491-500 ◽

Cited By ~ 35

Author(s):

E A Oosterom-Dragon ◽

H S Ginsberg

Keyword(s):

Protein Gene ◽

Temperature Sensitive ◽

Temperature Sensitive Mutants ◽

Two Temperature

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Characterization of a cell cycle mutant derived from hamster fibroblast: reversion analysis.

The Journal of Cell Biology ◽

10.1083/jcb.92.3.629 ◽

1982 ◽

Vol 92 (3) ◽

pp. 629-633 ◽

Cited By ~ 5

Author(s):

D J Scharff ◽

A M Delegeane ◽

A S Lee

Keyword(s):

Cell Line ◽

Mutant Cell ◽

Chinese Hamster ◽

Temperature Sensitive ◽

Nonpermissive Temperature ◽

Molecular Weights ◽

A Cell ◽

Ts Mutant ◽

Spontaneous Revertant

K12 is a temperature-sensitive (ts) mutant cell line derived from Chinese hamster fibroblasts. When incubated at the nonpermissive temperature, K12 cells exhibit the following properties: (a) the cells cannot initiate DNA synthesis;o (b) the synthesis of cytosol thymidine kinase is suppressed; and (c) the synthesis of three cellular proteins of molecular weights 94, 78, and 58 kdaltons is greatly enhanced. Here we characterize a spontaneous revertant clone, R12, derived from the K12 cells. We selected the revertant clone for its ability to grow at the nonpermissive temperature. Our results indicate that all the traits which constitute the K12 mutant phenotype are simultaneously reverted to the wild type in the revertant cell line, suggesting that the ts mutation of the K12 cells is of regulatory nature and exerts multiple effects on the expressed phenotypes.

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