Composition of Alginate Synthesized during the Growth Cycle of Pseudomonas aeruginosa

A mucoid P. aeruginosa isolated from the sputum of a cystic fibrosis patient was grown in batch culture on a complex medium. During the growth cycle the amount of alginate produced was estimated and its composition was determined by proton magnetic resonance (IH-n.m.r) spectroscopy. Exopolysaccharide production occurred mainly during the exponential phase of growth. The alginate samples isolated varied little in composition and were characterized by being highly acetylated, high mannuronate (0.83 - 0.93 mole fraction) polymers. Guluronate was present only within heteropolymeric regions of the polysaccharides which all displayed a complete absence of polyguluronate. Ca2 + ion supplementation of the medium was not observed to increase the levels of guluronate in the algi nates produced.

Download Full-text

Faculty Opinions recommendation of Environmental Pseudomonads Inhibit Cystic Fibrosis Patient-Derived Pseudomonas aeruginosa.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.727019009.793530084 ◽

2017 ◽

Author(s):

Joanna Goldberg ◽

Eryn E Bernardy

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Cystic Fibrosis Patient

Download Full-text

Serial isolates of Pseudomonas aeruginosa from a cystic fibrosis patient have identical pilin sequences.

Infection and Immunity ◽

10.1128/iai.56.3.665-672.1988 ◽

1988 ◽

Vol 56 (3) ◽

pp. 665-672 ◽

Cited By ~ 21

Author(s):

B L Pasloske ◽

A M Joffe ◽

Q Sun ◽

K Volpel ◽

W Paranchych ◽

...

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Cystic Fibrosis Patient

Download Full-text

Chronic infection sustained by a Pseudomonas aeruginosa High-Risk clone producing the VIM-1 metallo-β-lactamase in a cystic fibrosis patient after lung transplantation

Journal of Cystic Fibrosis ◽

10.1016/j.jcf.2018.01.007 ◽

2018 ◽

Vol 17 (4) ◽

pp. 470-474

Author(s):

Simona Pollini ◽

Claudia Mugnaioli ◽

Daniela Dolce ◽

Silvia Campana ◽

Anna Silvia Neri ◽

...

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

High Risk ◽

Lung Transplantation ◽

Cystic Fibrosis Patient ◽

Chronic Infection

Download Full-text

Complete Genome Sequence of Pseudomonas aeruginosa Strain AA2 (LMG 27630), an Early Isolate Recovered from the Airway of a German Cystic Fibrosis Patient

Microbiology Resource Announcements ◽

10.1128/mra.00526-20 ◽

2020 ◽

Vol 9 (26) ◽

Author(s):

Andrea Sass ◽

Tom Coenye

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Respiratory Tract ◽

Cystic Fibrosis Patient ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Opportunistic Pathogen ◽

Content Type ◽

Airway Infections ◽

Early Isolate

ABSTRACT Pseudomonas aeruginosa is an opportunistic pathogen that is able to cause various infections, including airway infections in cystic fibrosis patients. Here, we present the complete closed and annotated genome sequence of P. aeruginosa AA2, an isolate obtained early during infection of the respiratory tract of a German cystic fibrosis patient.

Download Full-text

Quantitative multivolume proton-magnetic resonance imaging in patients with cystic fibrosis lung disease: comparison with clinical indicators

European Respiratory Journal ◽

10.1183/13993003.02020-2017 ◽

2019 ◽

Vol 53 (5) ◽

pp. 1702020 ◽

Cited By ~ 4

Author(s):

Francesca Pennati ◽

Caterina Salito ◽

Irene Borzani ◽

Giulia Cervellin ◽

Simone Gambazza ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Cystic Fibrosis ◽

Magnetic Resonance ◽

Lung Disease ◽

Proton Magnetic Resonance ◽

Magnetic Resonance Images ◽

Imaging Biomarkers ◽

Resonance Imaging ◽

Clinical Indicators ◽

Proton Magnetic Resonance Imaging

This cross-sectional study aims to verify the relationship between quantitative multivolume proton-magnetic resonance imaging (1H-MRI) and clinical indicators of ventilatory abnormalities in cystic fibrosis (CF) lung disease.Non-enhanced chest MRI, spirometry and multiple breath washout was performed by 28 patients (10–27 years) with CF lung disease. Images acquired at end-inspiration and end-expiration were registered by optical flow to estimate expiratory–inspiratory proton-density change (Δ1H-MRI) as a measure of regional ventilation. Magnetic resonance images were also evaluated using a CF-specific scoring system.Biomarkers of CF ventilation impairment were defined from the Δ1H-MRI as follows: Δ1H-MRI median, Δ1H-MRI quartile coefficient of variation (QCV) and percentage of low-ventilation volume (%LVV). Imaging biomarkers correlated to all the clinical measures of ventilation abnormality, with the strongest correlation between Δ1H-MRI median and forced expiratory volume in 1 s (r2=0.44, p<0.001), Δ1H-MRI QCV and lung clearance index (LCI) (r2=0.51, p<0.001) and %LVV and LCI (r2=0.66, p<0.001). Correlations were also found between imaging biomarkers of ventilation and morphological scoring.The study showed a significant correlation between quantitative multivolume MRI and clinical indicators of CF lung disease. MRI, as a non-ionising imaging technique, may be particularly attractive in CF care for longitudinal evaluation, providing a new imaging biomarker to detect early ventilatory abnormalities.

Download Full-text