Onset and duration of stigma receptivity in Kunzea pomifera (Myrtaceae)

2006 ◽  
Vol 54 (6) ◽  
pp. 559 ◽  
Author(s):  
T. Page ◽  
G. M. Moore ◽  
J. Will ◽  
G. M. Halloran
Keyword(s):  
Pollen Tube ◽  
Reproductive Biology ◽  
Peroxidase Activity ◽  
Pollen Tube Growth ◽  
Seed Set ◽  
Stigma Receptivity ◽  
Controlled Pollination ◽  
Germinated Pollen ◽  
New Crop ◽  
Days After Anthesis

The onset and duration of stigma receptivity in K. pomifera was evaluated by observing stigma peroxidase activity, pollen-tube growth and seed set, following controlled pollination of flowers of different ages. Peroxidase activity was negligible from 1 day before to 4 days after anthesis, increasing to a peak of 65% at Day 13. The percentage of pistils bearing germinated pollen and ovaries exhibiting pollen-tube entry increased when pollen was applied to the stigma from the day before anthesis, to a maximum at Days 6 and 7 after anthesis, respectively, followed by a decline by Day 9. Under greenhouse conditions the optimum pollination period, measured as the level of set seed after pollination, ranged from 2 to 11 days after anthesis. The knowledge of this aspect of the species reproductive biology is of considerable value in controlled pollinations for its improvement as a new crop.

scholarly journals Effect of pistil age on pollen tube growth, fruit development and seed set in Cucurbita pepo L.

2014 ◽  
Vol 70 (3) ◽  
pp. 165-172 ◽  
Author(s):  
Ettore Pacini ◽  
Massimo Nepi
Keyword(s):  
Pollen Tube ◽  
Fruit Development ◽  
Pollen Tube Growth ◽  
Cucurbita Pepo ◽  
Seed Set ◽  
Fruit Weight ◽  
Tube Growth ◽  
Transmitting Tissue ◽  
Stigma Receptivity ◽  
Different Parts

The effects of pistil age on pollen tube growth, fruit development and seed set were studied in <em>Cucurbita pepo</em> L., the flower of which opens for only six hours. Stigma receptivity lasts four days, from one day before until two days after anthesis. Style receptivity lasts three days, from the day before to the day after anthesis. Ovule receptivity lasts two days: the day of anthesis and the day before. The rate of pollen tube growth varies in different parts of the pistil and in relation to pistil age. In the stigmatic and stylar region, the tubes grow faster if pollination occurs the day before anthesis; in the ovary they grow faster when pollination occurs at anthesis. In the receptacle region, where the transmitting tissue is reduced, the growth rate decreases independently of the time of pollination. The fruits are larger and heavier with more seeds when pollination occurs at anthesis. There is a positive correlation between seed number and fruit weight when pollination occurred at anthesis and the day before.

scholarly journals Ovarian Incompatibility in Tuberose (Polianthes Tuberosa L.) Cultivars and Breeding Lines

2021 ◽  
Author(s):  
Thangaraj Usha Bharathi ◽  
Rosalind Lallawmzuali ◽  
Subramanyamrao Prabhakarrao Kirthishree
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Normal Development ◽  
Seed Set ◽  
Interspecific Crosses ◽  
Self Incompatibility ◽  
Interaction Study ◽  
Nucellar Cell ◽  
Breeding Lines ◽  
Controlled Pollination

Abstract Background: Incompatibility occurs frequently in the plant system upon intra- or interspecific crosses resulting in several pollination barriers. The modest understanding on the breeding behaviour and mechanisms governing tuberose (Polianthes tuberosa L.) through this study will direct us to appraise the pollination and post pollination events on self- and cross- incompatibility and seed set behaviour for cross breeding programme.Results: Self-pollination of tuberose cultivars Arka Prajwal, Mexican Single and Arka Sugandhi failed to produce pods upon autogamy and geitonogamy. Pollen-pistil interaction study of incompatible cultivar Arka Prajwal substantiates that pollen tube enters the ovule within 24 hours after pollination. It discharges its contents into the embryo on the 1st day after pollination and polysaccharide granules evident upon fluorescence microscopic study. The pollen tube growth rates of self and controlled pollination were similar; however, sterility was expressed in the ovary. The female gametophytic development in self and cross incompatible cultivar Arka Prajwal showed normal development initially after pollination whereas embryosac degeneration was observed at later stage. Complete degeneration of the integument and nucellar cells was also observed. Conclusions: This study is the first to elucidate the occurrence of pseudo self-incompatibility in tuberose by identifying tuberose cultivars and breeding lines which produce pods upon geitonogamy. A positive pollen-pistil interaction with the degeneration of embryosac, integument and nucellar cell and pod shrinkage, abortion and fall confirms the prevalence of the ‘Ovarian Incompatibility’ or ‘Late-Acting Incompatibility’ in tuberose.

scholarly journals Pollination Biology of Hydrangea macrophylla

HortScience ◽  
2005 ◽  
Vol 40 (2) ◽  
pp. 335-338 ◽  
Author(s):  
Sandra M. Reed
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Stigma Receptivity ◽  
Hydrangea Macrophylla ◽  
Significant Difference ◽  
Days After Anthesis

Little information is available on the reproductive behavior of Hydrangea macrophylla (Thunb. Ex J.A. Murr.) Ser. The objectives of this study were to investigate time of stigma receptivity, viability of pollen from sterile flowers, and self-incompatibility in this popular ornamental shrub. Pollen germination and pollen tube growth in styles were examined using fluorescence microscopy. Stigma receptivity was examined in cross-pollinations made from 1 day before anthesis to 8 days after anthesis. Maximum stigma receptivity for the two cultivars examined occurred from anthesis to 4 days after anthesis. Viability of pollen from sterile flowers was evaluated through pollen staining and observations of pollen tube growth. No significant difference in percent stainable pollen between fertile and sterile flowers was observed in any of the six taxa examined. Pollen germination and pollen tube growth were studied in cross-pollinations made using pollen from fertile and sterile flowers of two cultivars. For both cultivars, pollen tubes from fertile and sterile flowers grew to the same length and had entered ovules by 72 hours after pollination. Self-incompatibility was evaluated by comparing pollen germination and pollen tube growth in cross- and self-pollinations. In the five taxa examined, self pollen tubes were significantly shorter than cross pollen tubes in flowers that were examined 72 hours after pollination. This finding indicates the presence of a gametophytic self-incompatibility system in H. macrophylla.

scholarly journals Reproductive biology in cassava: stigma receptivity and pollen tube growth

2019 ◽  
Vol 12 (1) ◽  
pp. 96-111 ◽  
Author(s):  
L.N. Ramos Abril ◽  
L.M. Pineda ◽  
I. Wasek ◽  
M. Wedzony ◽  
H. Ceballos
Keyword(s):  
Pollen Tube ◽  
Reproductive Biology ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Stigma Receptivity

scholarly journals Self-fertility and Time of Stigma Receptivity in Styrax japonicum

HortScience ◽  
2003 ◽  
Vol 38 (3) ◽  
pp. 429-431 ◽  
Author(s):  
Sandra M. Reed
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Fruit Set ◽  
The Self ◽  
Tube Growth ◽  
Stigma Receptivity ◽  
Days After Anthesis

The objectives of this study were to evaluate self-fertility and to determine the effectiveness of pollinations made over a 4-day period in Japanese snowbell, S. japonicum Sieb. & Zucc. Pollen germination and pollen tube growth were observed in stained styles following cross- and self-pollinations made from 1 day before to 2 days after anthesis. One month after pollination, fruit set averaged 40% in cross-pollinations and 14% in self-pollinations. Two months later, about one-third of the fruit resulting from cross-pollinations had aborted and only one fruit remained from the self-pollinations. This study demonstrated that stigmas of S. japonicum are receptive for at least 4 days and that flowers should be emasculated prior to making controlled cross-pollinations.

scholarly journals Pollination methods, stigma receptivity and pollen tube growth in Eucalyptus argophloia

2012 ◽  
Vol 61 (1-6) ◽  
pp. 121-126 ◽  
Author(s):  
Bruce W. Randall ◽  
David A. Walton ◽  
David J. Lee ◽  
Helen M. Wallace
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Stigma Receptivity ◽  
Pollination Success ◽  
One Stop ◽  
Fresh Pollen ◽  
The One ◽  
Pollination Methods ◽  
Days After Anthesis

AbstractEucalyptus argophloia Blakely (Western white gum) has shown potential as a commercial forestry timber species in marginal environments of north-eastern Australia. We measured early pollination success in Eucalyptus argophloia to compare pollination methods, determine the timing of stigma receptivity and compare fresh and stored pollen. Early pollination success was measured by counting pollen tubes in the style of E. argophloia 12 days after pollination. We compared the early pollination success of 1) Artificially Induced Protogyny (AIP), one-stop and three-stop methods of pollination; 2) flowers pollinated at 2 day intervals between 2 days before and 6 days after anthesis and 3) fresh pollen and pollen that had been stored for 9 months. Our results show significantly more pollen tubes from unpollinated AIP and AIP treatments than either the one-stop pollination or three-stop pollination treatments. This indicates that self-pollination occurs in the unpollinated AIP treatment. There was very little pollen tube growth in the one-stop method indicating that the three-stop method is the most suitable for this species. Stigma receptivity in E. argophloia commenced six days after anthesis and no pollen tube growth was observed prior to this. Fresh pollen resulted in pollen tube growth in the style whereas the stored pollen resulted in a total absence of pollen tube growth. We recommend that breeding programs incorporating E. argophloia as a female parent use the three-stop pollination method, and controlled pollination be carried out at least six days after anthesis using fresh pollen.

scholarly journals Stigmatic Receptivity Limits The Effective Pollination Period In Kiwifruit

1995 ◽  
Vol 120 (2) ◽  
pp. 199-202 ◽  
Author(s):  
Maria Victoria González ◽  
Manuel Coque ◽  
Maria Herrero
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Fruit Set ◽  
Limiting Factor ◽  
Stigma Receptivity ◽  
Factors Affecting ◽  
Different Ages ◽  
Days After Anthesis ◽  
Main Factor

The effective pollination period was determined in kiwifruit [Actinidia deliciosa (Chev.) Liang and Fergusonl and the factors affecting it were evaluated. The effective pollination period, measured as the capability to set fruit after hand-pollinating flowers of different ages, was 4 days; 5 days after anthesis fruit set decreased and 2 days later it was nil. Pollen tube growth did not appear to he a limiting factor since pollen tubes grew quickly and reached the base of the style 2 days after pollination and reached the ovules 1 day later. Ovules appeared viable for the 7 days following anthesis, and visibly degenerated within the following 3 days. Stigmatic receptivity was determined by the ability to sustain pollen germination after hand pollinating flowers of different ages. The duration of stigmatic receptivity closely fit the effective pollination period determined through fruit set. Thus, it appears that stigma receptivity is the main factor responsible for the short effective pollination period.

scholarly journals Transcriptome and translatome changes in germinated pollen under heat stress uncover roles of transporter genes involved in pollen tube growth

2020 ◽  
Author(s):  
Laetitia Poidevin ◽  
Javier Forment ◽  
Dilek Unal ◽  
Alejandro Ferrando
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Stress ◽  
High Temperature ◽  
Heat Shock ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Heat Shock Genes ◽  
Germinated Pollen

ABSTRACTPlant reproduction is one key biological process very sensitive to heat stress and, as a consequence, enhanced global warming poses serious threats to food security worldwide. In this work we have used a high-resolution ribosome profiling technology to study how heat affects both the transcriptome and the translatome of Arabidopsis thaliana pollen germinated in vitro. Overall, a high correlation between transcriptional and translational responses to high temperature was found, but specific regulations at the translational level were also present. We show that bona fide heat shock genes are induced by high temperature indicating that in vitro germinated pollen is a suitable system to understand the molecular basis of heat responses. Concurrently heat induced significant down-regulation of key membrane transporters required for pollen tube growth, thus uncovering heat-sensitive targets. We also found that a large subset of the heat-repressed transporters is specifically up-regulated, in a coordinated manner, with canonical heat-shock genes in pollen tubes grown in vitro and semi in vivo, based on published transcriptomes from Arabidopsis thaliana. Ribosome footprints were also detected in gene sequences annotated as non-coding, highlighting the potential for novel translatable genes and translational dynamics.

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