scholarly journals Pollination methods, stigma receptivity and pollen tube growth in Eucalyptus argophloia

2012 ◽  
Vol 61 (1-6) ◽  
pp. 121-126 ◽  
Author(s):  
Bruce W. Randall ◽  
David A. Walton ◽  
David J. Lee ◽  
Helen M. Wallace
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Stigma Receptivity ◽  
Pollination Success ◽  
One Stop ◽  
Fresh Pollen ◽  
The One ◽  
Pollination Methods ◽  
Days After Anthesis

AbstractEucalyptus argophloia Blakely (Western white gum) has shown potential as a commercial forestry timber species in marginal environments of north-eastern Australia. We measured early pollination success in Eucalyptus argophloia to compare pollination methods, determine the timing of stigma receptivity and compare fresh and stored pollen. Early pollination success was measured by counting pollen tubes in the style of E. argophloia 12 days after pollination. We compared the early pollination success of 1) Artificially Induced Protogyny (AIP), one-stop and three-stop methods of pollination; 2) flowers pollinated at 2 day intervals between 2 days before and 6 days after anthesis and 3) fresh pollen and pollen that had been stored for 9 months. Our results show significantly more pollen tubes from unpollinated AIP and AIP treatments than either the one-stop pollination or three-stop pollination treatments. This indicates that self-pollination occurs in the unpollinated AIP treatment. There was very little pollen tube growth in the one-stop method indicating that the three-stop method is the most suitable for this species. Stigma receptivity in E. argophloia commenced six days after anthesis and no pollen tube growth was observed prior to this. Fresh pollen resulted in pollen tube growth in the style whereas the stored pollen resulted in a total absence of pollen tube growth. We recommend that breeding programs incorporating E. argophloia as a female parent use the three-stop pollination method, and controlled pollination be carried out at least six days after anthesis using fresh pollen.

scholarly journals Pollination Biology of Hydrangea macrophylla

HortScience ◽  
2005 ◽  
Vol 40 (2) ◽  
pp. 335-338 ◽  
Author(s):  
Sandra M. Reed
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Stigma Receptivity ◽  
Hydrangea Macrophylla ◽  
Significant Difference ◽  
Days After Anthesis

Little information is available on the reproductive behavior of Hydrangea macrophylla (Thunb. Ex J.A. Murr.) Ser. The objectives of this study were to investigate time of stigma receptivity, viability of pollen from sterile flowers, and self-incompatibility in this popular ornamental shrub. Pollen germination and pollen tube growth in styles were examined using fluorescence microscopy. Stigma receptivity was examined in cross-pollinations made from 1 day before anthesis to 8 days after anthesis. Maximum stigma receptivity for the two cultivars examined occurred from anthesis to 4 days after anthesis. Viability of pollen from sterile flowers was evaluated through pollen staining and observations of pollen tube growth. No significant difference in percent stainable pollen between fertile and sterile flowers was observed in any of the six taxa examined. Pollen germination and pollen tube growth were studied in cross-pollinations made using pollen from fertile and sterile flowers of two cultivars. For both cultivars, pollen tubes from fertile and sterile flowers grew to the same length and had entered ovules by 72 hours after pollination. Self-incompatibility was evaluated by comparing pollen germination and pollen tube growth in cross- and self-pollinations. In the five taxa examined, self pollen tubes were significantly shorter than cross pollen tubes in flowers that were examined 72 hours after pollination. This finding indicates the presence of a gametophytic self-incompatibility system in H. macrophylla.

scholarly journals Self-fertility and Time of Stigma Receptivity in Styrax japonicum

HortScience ◽  
2003 ◽  
Vol 38 (3) ◽  
pp. 429-431 ◽  
Author(s):  
Sandra M. Reed
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Fruit Set ◽  
The Self ◽  
Tube Growth ◽  
Stigma Receptivity ◽  
Days After Anthesis

The objectives of this study were to evaluate self-fertility and to determine the effectiveness of pollinations made over a 4-day period in Japanese snowbell, S. japonicum Sieb. & Zucc. Pollen germination and pollen tube growth were observed in stained styles following cross- and self-pollinations made from 1 day before to 2 days after anthesis. One month after pollination, fruit set averaged 40% in cross-pollinations and 14% in self-pollinations. Two months later, about one-third of the fruit resulting from cross-pollinations had aborted and only one fruit remained from the self-pollinations. This study demonstrated that stigmas of S. japonicum are receptive for at least 4 days and that flowers should be emasculated prior to making controlled cross-pollinations.

Onset and duration of stigma receptivity in Kunzea pomifera (Myrtaceae)

2006 ◽  
Vol 54 (6) ◽  
pp. 559 ◽  
Author(s):  
T. Page ◽  
G. M. Moore ◽  
J. Will ◽  
G. M. Halloran
Keyword(s):  
Pollen Tube ◽  
Reproductive Biology ◽  
Peroxidase Activity ◽  
Pollen Tube Growth ◽  
Seed Set ◽  
Stigma Receptivity ◽  
Controlled Pollination ◽  
Germinated Pollen ◽  
New Crop ◽  
Days After Anthesis

The onset and duration of stigma receptivity in K. pomifera was evaluated by observing stigma peroxidase activity, pollen-tube growth and seed set, following controlled pollination of flowers of different ages. Peroxidase activity was negligible from 1 day before to 4 days after anthesis, increasing to a peak of 65% at Day 13. The percentage of pistils bearing germinated pollen and ovaries exhibiting pollen-tube entry increased when pollen was applied to the stigma from the day before anthesis, to a maximum at Days 6 and 7 after anthesis, respectively, followed by a decline by Day 9. Under greenhouse conditions the optimum pollination period, measured as the level of set seed after pollination, ranged from 2 to 11 days after anthesis. The knowledge of this aspect of the species reproductive biology is of considerable value in controlled pollinations for its improvement as a new crop.

scholarly journals Effect of pistil age on pollen tube growth, fruit development and seed set in Cucurbita pepo L.

2014 ◽  
Vol 70 (3) ◽  
pp. 165-172 ◽  
Author(s):  
Ettore Pacini ◽  
Massimo Nepi
Keyword(s):  
Pollen Tube ◽  
Fruit Development ◽  
Pollen Tube Growth ◽  
Cucurbita Pepo ◽  
Seed Set ◽  
Fruit Weight ◽  
Tube Growth ◽  
Transmitting Tissue ◽  
Stigma Receptivity ◽  
Different Parts

The effects of pistil age on pollen tube growth, fruit development and seed set were studied in <em>Cucurbita pepo</em> L., the flower of which opens for only six hours. Stigma receptivity lasts four days, from one day before until two days after anthesis. Style receptivity lasts three days, from the day before to the day after anthesis. Ovule receptivity lasts two days: the day of anthesis and the day before. The rate of pollen tube growth varies in different parts of the pistil and in relation to pistil age. In the stigmatic and stylar region, the tubes grow faster if pollination occurs the day before anthesis; in the ovary they grow faster when pollination occurs at anthesis. In the receptacle region, where the transmitting tissue is reduced, the growth rate decreases independently of the time of pollination. The fruits are larger and heavier with more seeds when pollination occurs at anthesis. There is a positive correlation between seed number and fruit weight when pollination occurred at anthesis and the day before.

scholarly journals Effective Pollination Period in Almond Cultivars

HortScience ◽  
2004 ◽  
Vol 39 (1) ◽  
pp. 19-22 ◽  
Author(s):  
E. Ortega ◽  
J. Egea ◽  
F. Dicenta
Keyword(s):  
High Temperature ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Fruit Set ◽  
Fruit Trees ◽  
Prunus Dulcis ◽  
Tube Growth ◽  
Stigma Receptivity ◽  
Flower Density

In almond [Prunus dulcis (Mill.) D.A. Webb], a high flower density and fruit set rate is important, because yield increases with higher fruit set ratio. Furthermore, because the ovule of some cultivars mature at anthesis, rapid pollination and pollen tube growth along the style are essential to ensure fertilization of a viable ovule. In this work, we studied the effective pollination period (EPP) of four almond cultivars of different bloom time by studying pollen tube growth and fruit set. EPP in almond was longer than in other fruit trees, and its duration was determined by stigma receptivity, which decreased with high temperature. An acceptable fruit set for all cultivars was obtained following pollination from day 0 to day 4 after emasculation.

scholarly journals Reproductive biology in cassava: stigma receptivity and pollen tube growth

2019 ◽  
Vol 12 (1) ◽  
pp. 96-111 ◽  
Author(s):  
L.N. Ramos Abril ◽  
L.M. Pineda ◽  
I. Wasek ◽  
M. Wedzony ◽  
H. Ceballos
Keyword(s):  
Pollen Tube ◽  
Reproductive Biology ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Stigma Receptivity

scholarly journals An Evaluation of Fertility in Arkansas Primocane-fruiting Blackberries

HortScience ◽  
2010 ◽  
Vol 45 (7) ◽  
pp. 1000-1005 ◽  
Author(s):  
Alisha L. Ruple ◽  
John R. Clark ◽  
M. Elena Garcia
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Fruit Set ◽  
Pollen Viability ◽  
Tube Growth ◽  
Stigma Receptivity ◽  
Cross Pollination ◽  
Self Pollination ◽  
Selection Of

Floral fertility of five primocane-fruiting (PF) blackberry (Rubus L. subgenus Rubus Watson) genotypes (‘Prime-Jim’®, APF-31, ‘Prime-Ark® 45’, APF-59, and APF-77) and three floricane-fruiting (FF) genotypes (‘Navaho’, ‘Natchez’, and ‘Ouachita’) were tested under field conditions using floricane flowers with four pollination treatments: undisturbed open-pollinated, emasculated self-pollinated, emasculated and cross-pollinated with pollen from a similar fruiting type (PF × PF or FF × FF), and emasculated and cross-pollinated with pollen from a different fruiting type (PF × FF or FF × PF). During primocane flowering, three pollination treatments (undisturbed open-pollinated, emasculated selfed, and emasculated cross-pollinated) were used to further test the fertility of the five PF genotypes. Significant differences between cross-pollination treatments and self-pollination were seldom noted with more differences seen in ‘Prime-Jim’® than any other genotype. Cross-pollinating primocane flowers on ‘Prime-Jim’® resulted in significantly higher fruit set, drupelet set, and average berry weight compared with self-pollination. Fruit set among genotypes ranged from 68.5 to 96.7%, and drupelet set rating ranged from 4.3 to 6.9 for floricane flowers. For open-pollinated primocane flowers, fruit set ranged from 63.9 to 92.1%, and drupelet set rating ranged from 4.3 to 7.2. The genotypes APF-31, APF-59, and APF-77 showed a marked improvement over ‘Prime-Jim’® in both percent fruit set and drupelet set of floricane and primocane flowers. The results indicated that fertility appears to be sufficient in all the genotypes evaluated and that the later-generation PF genotypes show improvement in fertility over ‘Prime-Jim’®. Pollen viability (using both chemical viability testing and in vitro germination testing), stigma receptivity, and pollen tube growth (using florescence) were evaluated in a controlled environment to determine if any improvements could be noticed when comparing a selection of later-generation PF genotypes (APF-31,' Prime-Ark® 45', APF-59, and APF-77) with ‘Prime-Jim’® and ‘Prime-Jan’®. Genotypes APF-31, APF-59, and APF-77 had significantly more viable pollen and pollen germination than ‘Prime-Jan’®. Stigma receptivity was observed in all genotypes. Pollen tube growth did not appear to be inhibited after self-pollination in any genotype studied, although significantly greater florescence resulting from pollen tube growth was seen after cross-pollination compared with self-pollination for APF-59. There were no significant differences in pollen tube growth between cross- and self-pollination for any other genotype.

Interspecific and Intergeneric Pistil - Pollen Compatibility of Banksia coccinea (Proteaceae)

1998 ◽  
Vol 46 (4) ◽  
pp. 453 ◽  
Author(s):  
T. L. Maguire ◽  
M. Sedgley
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Related Genus ◽  
Reciprocal Effect ◽  
Pollination Success ◽  
Intergeneric Crosses ◽  
Close Relationship ◽  
Species Groups ◽  
The Relationship

Interspecific and intergeneric pollen-tube growth was investigated using controlled hand pollinations of Banksia coccinea R.Br., 35 species of Banksia L.f., and three species of the related genus Dryandra R.Br. Currently, the relationship between B. coccinea and other species groups within Banksia is unclear. Some species supported no germination of B. coccinea pollen, some supported normal pollen-tube growth and others produced pollen-tube abnormalities including thickened walls, bulbous swellings, directionless growth, burst tips and branched tubes. Control of pollen-tube growth in the pistil was imposed in the pollen presenter and upper style. There was no significant reciprocal effect on pollination success in the lower style. The results of pollen-tube compatability in the lower style indicated that B. coccinea has a closer affinity to the section Oncostylis, than the section Banksia where it is currently placed. Intergeneric crosses of B. coccinea with Dryandra species resulted in some compatibility, with one cross having low numbers of pollen-tubes in the pollen presenter and upper style region. These results indicate a close relationship between Banksia and Dryandra, which are sister genera in the tribe Banksiae, family Proteaceae.

scholarly journals Stigmatic Receptivity Limits The Effective Pollination Period In Kiwifruit

1995 ◽  
Vol 120 (2) ◽  
pp. 199-202 ◽  
Author(s):  
Maria Victoria González ◽  
Manuel Coque ◽  
Maria Herrero
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Fruit Set ◽  
Limiting Factor ◽  
Stigma Receptivity ◽  
Factors Affecting ◽  
Different Ages ◽  
Days After Anthesis ◽  
Main Factor

The effective pollination period was determined in kiwifruit [Actinidia deliciosa (Chev.) Liang and Fergusonl and the factors affecting it were evaluated. The effective pollination period, measured as the capability to set fruit after hand-pollinating flowers of different ages, was 4 days; 5 days after anthesis fruit set decreased and 2 days later it was nil. Pollen tube growth did not appear to he a limiting factor since pollen tubes grew quickly and reached the base of the style 2 days after pollination and reached the ovules 1 day later. Ovules appeared viable for the 7 days following anthesis, and visibly degenerated within the following 3 days. Stigmatic receptivity was determined by the ability to sustain pollen germination after hand pollinating flowers of different ages. The duration of stigmatic receptivity closely fit the effective pollination period determined through fruit set. Thus, it appears that stigma receptivity is the main factor responsible for the short effective pollination period.

Inhibition of Pollen Tube Growth by Diffusate and Extract of Grape Pistils

1989 ◽  
Vol 58 (3) ◽  
pp. 515-521 ◽  
Author(s):  
Goro OKAMOTO ◽  
Ikuo SHIBUYA ◽  
Miwa FURUICHI ◽  
Kazuo SHIMAMURA
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth
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