Effect of nickel on pollen germination and pollen tube length in Arabis alpina (Brassicaceae)

2016 ◽  
Vol 64 (4) ◽  
pp. 302 ◽  
Author(s):  
Dolja Pavlova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Tube Length ◽  
Serpentine Soils ◽  
Nutritional Medium ◽  
Arabis Alpina ◽  
Pollen Tube Elongation ◽  
Germinated Pollen ◽  
Pollen Tube Length

In this work we studied and compared the toxic effect of nickel (Ni) on pollen germination and pollen tube length in Arabis alpina L. collected from serpentine and non-serpentine populations distributed in the Rila mountains, Bulgaria. Pollen grains were treated with prepared standard solutions of 100, 300, 500, and 700 μM Ni as NiCl2 in distilled water. A nutritional medium was also used to assess pollen germination. Nickel inhibited pollen germination and pollen tube elongation in both serpentine and non-serpentine plants. The percentage of germinated pollen in serpentine plants treated with Ni was higher than in non-serpentine plants but there was no difference in pollen tube elongation between groups. However, pollen tubes showed abnormalities such as coiling and swelling of the tip, or burst, and varied considerably among the samples. A complete break of pollen tube elongation is due to such abnormalities. Also, decreased pollen fertility in both populations was found. The plants from serpentines were less sensitive to (i.e. more tolerant of) elevated Ni concentrations commonly found in serpentine soils.

scholarly journals Effect of morphactin (IT 3456) on pollen germination and pollen tube growth in four species of Angiospermae

2014 ◽  
Vol 51 (2) ◽  
pp. 179-185
Author(s):  
Barbara Gabara
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Tube Growth ◽  
Tube Length ◽  
Antirrhinum Majus ◽  
Eschscholtzia Californica ◽  
Germination Process ◽  
Narcissus Poeticus ◽  
Pollen Tube Length

The action of morphactin in 10, 100 and 1000 ppm on pollen germination and tube growth was studied in four species: <em>Antirrhinum majus, Campanula rapunculoides, Eschscholtzia californica</em> and <em>Narcissus poeticus</em>. Pollen grains showed different sensitivity to particular concentrations of morphactin. Although the concentration of 1000 ppm inhibited completely pollen germination in <em>A. majus</em> and <em>C. rapunculoides</em>, it was not sufficient to suppress this process in <em>E. californica</em> and <em>N. poeticus</em>. Morphactin has a different effect on the percentage of germinated and ruptured pollen, tube length and its rate of growth. This effect was either inhibitory, neutral or stimulatory, depending on the morphactin concentration, the time of culture and species studied. The action of morphactin on the germination process is discussed.

scholarly journals Effects of fluorides and sulphur dioxide on pollen germination and growth of the pollen tube

2014 ◽  
Vol 54 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Leon Mejnartowicz ◽  
Andrzej Lewandowski
Keyword(s):  
Air Pollution ◽  
Pollen Tube ◽  
Sulphur Dioxide ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Phosphate Fertilizer ◽  
Tube Length ◽  
Pine Pollen ◽  
Germination And Growth ◽  
Pollen Tube Length

The action of fluorides and sulphur dioxides from emissions from a phosphate fertilizer factory significantly reduced the germination of Scots pine pollen grains. The pollen tube length indicated that its growth is uninhibited even though the pollen was collected under conditions of air-pollution. There are statistically significant differences showing longer tubes in the sensitive trees pollen grains. l he ageing of pollen from the sensitive trees occurs probably more rapidly than in the tolerant trees.

Differences in Style Length and In vitro Germinated Pollen Tube Length and Other Reproductive Structures of Tetraploid and Diploid Cottons

2000 ◽  
Vol 3 (9) ◽  
pp. 1372-1374
Author(s):  
Mohammed Jurial Baloch . ◽  
Abdul Rahim Lakho . ◽  
Hidayatullah Bhutto . ◽  
Mohammad Hussain Arain .
Keyword(s):  
Pollen Tube ◽  
Tube Length ◽  
Style Length ◽  
Reproductive Structures ◽  
Germinated Pollen ◽  
Pollen Tube Length

GENOTYPIC DIFFERENCES IN CARDINAL TEMPERATURES FOR IN VITRO POLLEN GERMINATION AND POLLEN TUBE GROWTH OF COCONUT HYBRIDS

2017 ◽  
Vol 54 (5) ◽  
pp. 731-743 ◽  
Author(s):  
C. S. RANASINGHE ◽  
M. D. P. KUMARATHUNGE ◽  
K. G. S. KIRIWANDENIYA
Keyword(s):  
High Temperature ◽  
Sri Lanka ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Fruit Set ◽  
Tube Growth ◽  
Tube Length ◽  
Cardinal Temperatures ◽  
Pollen Tube Length

SUMMARYSuccessful fruit set in coconut depends on several reproductive processes including pollen germination and pollen tube growth. High temperature (˃33 °C) during flowering reduces fruit set in coconut. Therefore, identification and development of coconut varieties or hybrids with high reproductive heat tolerance will benefit the coconut industry in view of the climate changes. This experiment was conducted to quantify the response of pollen germination and pollen tube growth of seven coconut hybrids to increasing temperature from 16 to 38 °C. A Principal Component Analysis (PCA) was carried out to classify coconut hybrids on the basis of their temperature tolerances to pollen germination. Pollen germination and pollen tube length of the hybrids ranged from 56 to 78% and 242 to 772 µm, respectively. A modified bilinear model best described the response to temperature of pollen germination and pollen tube length. Cardinal temperatures (Tmin, Topt and Tmax) of pollen germination and pollen tube length varied among the seven hybrids. PCA identified Tmax for pollen germination and Topt for pollen tube length as the most important parameters in describing varietal tolerance to high temperature. PCA also identified SLGD × Sri Lanka Tall and Sri Lanka Brown Dwarf × Sri Lanka Tall as the most tolerant hybrids to high temperature stress and Sri Lanka Tall × Sri Lanka Tall and Sri Lanka Green Dwarf × San Ramon as less tolerant ones based on cardinal temperatures for pollen germination and pollen tube length. Tmax for pollen germination of the most tolerant and less tolerant hybrids were 41.9 and 39.5 °C, respectively. Topt for pollen tube length in the most tolerant and less tolerant hybrids were 29.5 and 26.0 °C, respectively.

scholarly journals In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

2017 ◽  
Vol 32 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Natalia Georgieva ◽  
Ivelina Nikolova ◽  
Valentin Kosev ◽  
Yordanka Naydenova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
In Vitro Germination ◽  
Pisum Sativum L ◽  
Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

In vitro germination and pollen tube growth of maize (Zea mays) pollen. II. Pollen source, calcium, and boron interactions

1968 ◽  
Vol 46 (3) ◽  
pp. 235-240 ◽  
Author(s):  
P. L. Pfahler
Keyword(s):  
Zea Mays ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Calcium Nitrate ◽  
Pollen Grains ◽  
Germination Percentage ◽  
Tube Growth ◽  
Tube Length ◽  
Pollen Tube Length

Pollen grains from six single cross hybrids (Wf9 × H55, Wf9 × H50, H49 × H55, H49 × H50, Ky49 × Ky27, and K64 × K55) of Zea mays L. were cultured on an artificial medium containing 15% sucrose and 0.6% bacto-agar supplemented with all possible combinations of two levels (0.00 and 0.03%) of calcium nitrate (Ca) and two levels (0.00 and 0.01%) of boric acid (B). The effect of Ca and B on germination percentage was found to be dependent on the genetic source of the pollen. At the 0.00% levels of Ca and B, the germination percentage of the hybrids ranged from 26.6% for Ky49 × Ky27 to 58.1% for K64 × K55. With either Ky49 × Ky27 or K64 × K55 pollen, the addition of Ca and B to the medium resulted in a lower germination percentage whereas the addition of Ca and B increased the germination percentage of the other hybrids. The addition of B in the absence of Ca sharply reduced the germination percentage in all hybrids while in the presence of Ca, the effect of B varied with the hybrid involved. For pollen tube length, highly significant interactions between hybrids and levels of Ca and B were found. However, maximum pollen tube lengths were obtained for all hybrids with both Ca and B in the medium. The addition of B in the absence of Ca reduced the pollen tube length of all hybrids whereas the addition of B in the presence of Ca increased pollen tube length. In general, the addition of both Ca and B increased the rate of pollen tube growth especially in the period 2 to 3 hours after inoculation.

scholarly journals POLLUTION AND DEVELOPMENT OF TOMATO POLLEN

HortScience ◽  
1992 ◽  
Vol 27 (11) ◽  
pp. 1163e-1163
Author(s):  
Yuan-Hai Zhang ◽  
Lyle E. Craker
Keyword(s):  
Air Pollution ◽  
Acid Rain ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Tube Length ◽  
Lycopersicon Hirsutum ◽  
Tomato Plants ◽  
Gametophytic Selection ◽  
Lycopersicon Pennellii ◽  
Pollen Tube Length

Air pollution may play a role in gametophytic selection. To estimate whether such selection was occurring, pollen grains from homozygous and heterozygous tomato plants were tested under pollution stress. Homozygous pollen could be expected to respond to pollution more uniformly than heterozygous due to the identical genotype of the pollen grains. Acid rain reduced pollen germination and tube elongation in Lycopersicon hirsutum LA1777 (heterozygous) and Lycopersicon pennellii LA716 (nearly homozygous). UV-B reduced tube length of the pollen from both plants, but ozone only reduced pollen tube length of L. pennellii. The responses of these two kinds of pollen to acid rain, ozone, and UV-B appears to be same in terms of heterozygosity and stress dosages, suggesting the reduction of pollen germination and tube elongation under pollution stress may be mediated through physiological or physical alterations and not a response of different genotypes.

IN VITRO GERMINATION AND POLLEN TUBE GROWTH OF MAIZE (ZEA MAYS L.) POLLEN.: I. CALCIUM AND BORON EFFECTS

1967 ◽  
Vol 45 (6) ◽  
pp. 839-845 ◽  
Author(s):  
P. L. Pfahler
Keyword(s):  
Pollen Tube ◽  
Boric Acid ◽  
Calcium Nitrate ◽  
Pollen Grains ◽  
Germination Percentage ◽  
Tube Length ◽  
Artificial Medium ◽  
Bacto Agar ◽  
Pollen Tube Length

Pollen grains from the single cross hybrid Wf9 × H55 were cultured on an artificial medium containing 15% sucrose and 0.6% bacto-agar supplemented with all possible combinations of three concentrations (0.00, 0.03, and 0.06%) of calcium nitrate and three concentrations (0.00, 0.01, and 0.02%) of boric acid. The combination containing 0.03% calcium nitrate and 0.01% boric acid produced the highest germination (47.9%) and the longest tube length (405 μ). The addition of either calcium nitrate or boric acid alone decreased the germination percentage and pollen tube length. The percentage of grains producing more than one pollen tube was reduced by the addition of calcium nitrate or boric acid singly or in combination. The significance of these results in relation to pollen physiology was discussed.

scholarly journals Vitality and in vitro pollen germination of different ‘Oblacinska’ sour cherry clones

Genetika ◽  
2017 ◽  
Vol 49 (3) ◽  
pp. 791-800
Author(s):  
Milica Fotiric-Aksic ◽  
Radosav Cerovic ◽  
Vera Rakonjac ◽  
Ivana Bakic ◽  
Slavica Colic ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Fruit Set ◽  
Sour Cherry ◽  
Tube Growth ◽  
Tube Length ◽  
In Vitro Pollen Germination ◽  
Pollen Tube Length

Vitality of pollen, in vitro pollen germination and pollen tube growth (pollen tube length and pollen tube growth rate) were investigated in Oblacinska sour cherry in order to determine the differences between clones which have divergent yielding potential. For this purpose two ?Oblacinska? sour cherry clones with high fruit set and high yields (II/2, III/9) and two with low fruit set and low-yielding (XI/3 and XIII/1) were used in this study. Pollen germination was done on artificial medium containing 14% sucrose and 0.3% agar-agar at room temperature (23?C). Pollen tube growth was stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact with the medium. The maximum percentage of germination ranged from 13.01% (clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube length varied from 64.84 ?m (clone XIII/1, after 1 h) to >1,100 ?m (clones II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 ?m min-1) after 6 h of germination, but rather decreasing until 24 h of germination (0.56-0.83 ?m min-1). The dynamics of in vitro pollen tubes growth among the clones were quite different, especially after 12 h and 24 h of germination. Clones that are singled out as fruitful (II/2 and III/9) gave much better results regarding pollen germination and pollen tube growth in comparison to clones which were characterized by low fruit set and yields (XI/3 and XIII/1).

scholarly journals Pollen viability in Quercus robur L.

2017 ◽  
Vol 69 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Branislava Batos ◽  
Danijela Miljkovic
Keyword(s):  
Pollen Tube ◽  
Quercus Robur ◽  
Interindividual Variability ◽  
Germination Rate ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Tube Length ◽  
Pedunculate Oak ◽  
Pollen Tube Length

The variability of viability (germination rate and the length of pollen tubes) of fresh pedunculate oak (Quercus robur L.) pollen grains was studied in vitro on a medium containing 15% sucrose. Spatial variability was studied by sampling fresh pollen grains from a total of thirteen trees at four different sites in the area of Belgrade (Kosutnjak, Banovo Brdo, Ada Ciganlija and Bojcin Forest) in a single year (2011). In order to assess temporal variability and determine the effects of climate change on a small time scale, we studied the viability of the pollen grains collected from one tree at the Banovo Brdo site in six different years (2004, 2005, 2006, 2007, 2011 and 2012). Interindividual variability was tested on the pollen grains sampled from eight trees at Ada Ciganlija in 2004. The percentage values of the pollen grain germination rate and the pollen tube length showed no statistically significant differences between the sites. However, the studied characteristics of the pollen grain viability (germination rate and pollen tube length) showed statistically significant differences in both temporal (between the pollen collection years) and interindividual variability. This type of research makes a valuable contribution to pedunculate oak breeding programs through the identification of trees with stable production and a good quality of pollen. Furthermore, it can be important in defining the patterns of spatial, temporal and individual variability of pollen grain viability under the influence of climate factors, which are showing compelling changing trends from year to year.

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