Pollen viability in Quercus robur L.

The variability of viability (germination rate and the length of pollen tubes) of fresh pedunculate oak (Quercus robur L.) pollen grains was studied in vitro on a medium containing 15% sucrose. Spatial variability was studied by sampling fresh pollen grains from a total of thirteen trees at four different sites in the area of Belgrade (Kosutnjak, Banovo Brdo, Ada Ciganlija and Bojcin Forest) in a single year (2011). In order to assess temporal variability and determine the effects of climate change on a small time scale, we studied the viability of the pollen grains collected from one tree at the Banovo Brdo site in six different years (2004, 2005, 2006, 2007, 2011 and 2012). Interindividual variability was tested on the pollen grains sampled from eight trees at Ada Ciganlija in 2004. The percentage values of the pollen grain germination rate and the pollen tube length showed no statistically significant differences between the sites. However, the studied characteristics of the pollen grain viability (germination rate and pollen tube length) showed statistically significant differences in both temporal (between the pollen collection years) and interindividual variability. This type of research makes a valuable contribution to pedunculate oak breeding programs through the identification of trees with stable production and a good quality of pollen. Furthermore, it can be important in defining the patterns of spatial, temporal and individual variability of pollen grain viability under the influence of climate factors, which are showing compelling changing trends from year to year.

Download Full-text

Effects of storage temperature and duration on pollen grain viability and pollen-tube elongation in Chinese chinqapin (Castanea henryi Skan)

Bangladesh Journal of Botany ◽

10.3329/bjb.v49i2.49310 ◽

2020 ◽

Vol 49 (2) ◽

pp. 297-304

Author(s):

Huan Xiong ◽

Deyi Yuan ◽

Zhi-Yu Deng ◽

Genhua Niu ◽

Feng Zou

Keyword(s):

Pollen Tube ◽

Room Temperature ◽

Storage Temperature ◽

Southern China ◽

Germination Rate ◽

Pollen Viability ◽

Pollen Grain ◽

Tube Length ◽

Storage Duration ◽

Pollen Tube Length

Chinese chinquapin [Castanea henryi (Skan) Rehder & E.H. Wilson] is used as a food and timber crop in southern China. Most chinquapin cultivars are self-incompatible and bloom at different times; consequently, artificial pollination is used to ensure fruit set and nut yield. Effective pollen storage that enables producers and breeders to use stored pollen for cross-pollination at a later date is important. In this study, the cultivar Changmangzi was used to estimate the viability and pollen tube length of pollen stored at room temperature, and at 4, −20, and −80°C using in vitro germination tests. It was observed that pollen grain germination significantly decreased at all four storage temperatures. Pollen viability was 14.4% after only 24 days of storage at room temperature. The germination rate was 13.3% after 90 days of storage at 4°C, and 14.5% after 180 days at −20°C. The initial germination rate of pollen stored at −80°C was 56.3% at the beginning of the test and decreased to 15.4% after 240 days. Pollen-tube length decreased with increased storage duration; mean pollen-tube lengths ranged from 109.44 to 257.51 μm. Based on these results, it is suggested that a storage temperature of −80°C for Changmangzi pollen is good.

Download Full-text

Pollen morphology and viability of some Indian mangroves

Annals of Tropical Research ◽

10.32945/atr3015.2008 ◽

2008 ◽

pp. 60-71 ◽

Cited By ~ 1

Author(s):

Suparna Gupta ◽

Arunima Ghosh ◽

Subrata Maity ◽

Sauren Das

Keyword(s):

Pollen Tube ◽

Pollen Morphology ◽

Pollen Grains ◽

Pollen Grain ◽

Tube Length ◽

Strong Positive Correlation ◽

Viable Pollen ◽

The Family ◽

Indian Mangroves ◽

Pollen Tube Length

A study on pollen morphology of four common Indian mangroves from Sundarbans mangrove swamps, three species from the family Rhizophoraceae (Bruguiera sexangula Laur., Ceriops tagal Perr.and Rhizophora mucronata Lamk.) and one species from the family Avicenniaceae (Avicennia marina Forsk.) was carried out. Shape of the pollen grains were prolate, prolate spheroidal and subprolate with tricolporate apertures. Surface ornamentation of the studied taxa was reticulate, finely reticulate or scabrate. Pollen grain viability of the said taxa was determined by stain test (1% aceto-orcein) under in situ condition and percentage of viable pollen production was recorded. The result revealed that all the studied taxa produce a fairly good amount of viable pollen grains in natural condition, an indication that they are good propagule producer in the long run. Pollen grain germination and measurements of pollen tube length using different sucrose concentrations (0.2, 0.6, 1.0, 1.2, 1.6 and 2.0%). Maximum pollen fertility and pollen tube length occurred in 2% sucrose solution in case of A. marina and B. sexangula, whereas 1.6% in C. tagal and 1.0% in R. mucronata. A strong positive correlation exist between pollen germination and pollen tube length, but there was a hardly relation between sucrose concentration and pollen tube length.

Download Full-text

In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

Pesticidi i fitomedicina ◽

10.2298/pif1701061g ◽

2017 ◽

Vol 32 (1) ◽

pp. 61-65 ◽

Cited By ~ 1

Author(s):

Natalia Georgieva ◽

Ivelina Nikolova ◽

Valentin Kosev ◽

Yordanka Naydenova

Keyword(s):

Pollen Tube ◽

Pollen Germination ◽

Culture Media ◽

Pollen Viability ◽

Pollen Grains ◽

Pollen Grain ◽

In Vitro Germination ◽

Pisum Sativum L ◽

Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

Download Full-text

Effect of nickel on pollen germination and pollen tube length in Arabis alpina (Brassicaceae)

Australian Journal of Botany ◽

10.1071/bt15291 ◽

2016 ◽

Vol 64 (4) ◽

pp. 302 ◽

Cited By ~ 1

Author(s):

Dolja Pavlova

Keyword(s):

Pollen Tube ◽

Pollen Germination ◽

Pollen Grains ◽

Tube Length ◽

Serpentine Soils ◽

Nutritional Medium ◽

Arabis Alpina ◽

Pollen Tube Elongation ◽

Germinated Pollen ◽

Pollen Tube Length

In this work we studied and compared the toxic effect of nickel (Ni) on pollen germination and pollen tube length in Arabis alpina L. collected from serpentine and non-serpentine populations distributed in the Rila mountains, Bulgaria. Pollen grains were treated with prepared standard solutions of 100, 300, 500, and 700 μM Ni as NiCl2 in distilled water. A nutritional medium was also used to assess pollen germination. Nickel inhibited pollen germination and pollen tube elongation in both serpentine and non-serpentine plants. The percentage of germinated pollen in serpentine plants treated with Ni was higher than in non-serpentine plants but there was no difference in pollen tube elongation between groups. However, pollen tubes showed abnormalities such as coiling and swelling of the tip, or burst, and varied considerably among the samples. A complete break of pollen tube elongation is due to such abnormalities. Also, decreased pollen fertility in both populations was found. The plants from serpentines were less sensitive to (i.e. more tolerant of) elevated Ni concentrations commonly found in serpentine soils.

Download Full-text

Assessment of Pollen Viability, Germination, and Tube Growth in Eight Tunisian Caprifig (Ficus carica L.) Cultivars

ISRN Agronomy ◽

10.1155/2013/207434 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4 ◽

Cited By ~ 16

Author(s):

Badii Gaaliche ◽

Afifa Majdoub ◽

Mehdi Trad ◽

Messaoud Mars

Keyword(s):

Pollen Tube ◽

Pollen Tube Growth ◽

Germination Rate ◽

Pollen Viability ◽

Tube Growth ◽

Tube Length ◽

Tetrazolium Chloride ◽

North West ◽

Ttc Test

The evaluation of pollen viability and its germination capacity are two essential criteria for pollinator’s characterization. This study was carried out to evaluate pollen quality of eight caprifigs grown in the center-east and north-west of Tunisia. Two colorimetric tests 2,3,5-triphenyl tetrazolium chloride (TTC) and acetocarmine were used to estimate pollen viability. Germination rate and pollen tube growth in a culture medium containing 5% sucrose, 5 ppm boric acid (H3BO3) and 1% agar were registered after 24, 48, and 72 hours (H) of incubation. Results showed that the highest pollen viability rate (84%) was obtained by TTC test in caprifig Assafri, followed by Jrani (80.2%), Djebba 2 (77.8%), and Djebba 1 (73.6%). That of other caprifigs did not exceed 50% for the two tests. In all caprifig types, germination rate and pollen tube growth varied according to the incubation period. The highest percentage of germination (72%) and maximum pollen tube length (960 μm) were recorded after 72 H of incubation in caprifigs Assafri and Jrani, respectively. Among the caprifigs studied, four types (Assafri, Jrani, Djebba 1, and Djebba 2) appeared to be suitable pollinators with respect to the criteria investigated.

Download Full-text

In vitro germination and pollen tube growth of maize (Zea mays) pollen. II. Pollen source, calcium, and boron interactions

Canadian Journal of Botany ◽

10.1139/b68-039 ◽

1968 ◽

Vol 46 (3) ◽

pp. 235-240 ◽

Cited By ~ 15

Author(s):

P. L. Pfahler

Keyword(s):

Zea Mays ◽

Pollen Tube ◽

Pollen Tube Growth ◽

Calcium Nitrate ◽

Pollen Grains ◽

Germination Percentage ◽

Tube Growth ◽

Tube Length ◽

Pollen Tube Length

Pollen grains from six single cross hybrids (Wf9 × H55, Wf9 × H50, H49 × H55, H49 × H50, Ky49 × Ky27, and K64 × K55) of Zea mays L. were cultured on an artificial medium containing 15% sucrose and 0.6% bacto-agar supplemented with all possible combinations of two levels (0.00 and 0.03%) of calcium nitrate (Ca) and two levels (0.00 and 0.01%) of boric acid (B). The effect of Ca and B on germination percentage was found to be dependent on the genetic source of the pollen. At the 0.00% levels of Ca and B, the germination percentage of the hybrids ranged from 26.6% for Ky49 × Ky27 to 58.1% for K64 × K55. With either Ky49 × Ky27 or K64 × K55 pollen, the addition of Ca and B to the medium resulted in a lower germination percentage whereas the addition of Ca and B increased the germination percentage of the other hybrids. The addition of B in the absence of Ca sharply reduced the germination percentage in all hybrids while in the presence of Ca, the effect of B varied with the hybrid involved. For pollen tube length, highly significant interactions between hybrids and levels of Ca and B were found. However, maximum pollen tube lengths were obtained for all hybrids with both Ca and B in the medium. The addition of B in the absence of Ca reduced the pollen tube length of all hybrids whereas the addition of B in the presence of Ca increased pollen tube length. In general, the addition of both Ca and B increased the rate of pollen tube growth especially in the period 2 to 3 hours after inoculation.

Download Full-text

Longevity and germination of Juniperus communis L. pollen after storage

Scientific Reports ◽

10.1038/s41598-021-90942-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Andrej Kormuťák ◽

Peter Bolecek ◽

Martin Galgóci ◽

Dušan Gömöry

Keyword(s):

Pollen Tube ◽

Pollen Viability ◽

Germination Percentage ◽

Tube Length ◽

In Vitro Germination ◽

Short Term ◽

Term Storage ◽

Pollen Tube Length ◽

Common Juniper

AbstractPollen storage belongs among the most important activities associated with pollen handling. It overcomes the differences in pollen shedding and ovule receptivity during controlled pollination experiments. It is especially important for species like common juniper (Juniperus communis L.) with an extremely low quality of seeds due to pollination failure. Additionally, it is a substantial part of germplasm preservation programmes in pollen banks. In the present paper, the effect of short-term storage of pollen was studied using pollen samples from five shrubs in an in vitro germination test. Two temperature regimes were tested. The pollen viability of freshly collected pollen varied considerably between individual shrubs, exhibiting 67.3–88.6% germination rate and 248.0–367.3 µm of pollen tubes. Storage at + 4 °C for four months was accompanied by a profound decline in pollen viability. The germination percentage was reduced to 49.2–75.2% and the pollen tube length to 32.5–69.0%, depending on individual shrubs. The corresponding decline in pollen viability characteristics during storage at − 20 °C was only negligible in two of the tested shrubs. In the remaining three shrub samples, an increase in germination percentage was observed. Pollen tube growth responded more sensitively to freezing, but, on average, the decrease in length was lower than that at + 4 °C. The rate of reduction in pollen tube length varied between 11.5 and 45.4%. Cytological events accompanying in vitro germination of freezer-stored pollen exhibited some delay in releasing the exine from pollen grains during the early stages of germination as compared with freshly collected pollen. In conclusion, short-term storage of the common juniper pollen in a freezer is better for the preservation of its viability than storage at + 4 °C.

Download Full-text

Assessment of pollen viability and germination in Swainsona formosa (G.Don) J.Thompson

Biospecies ◽

10.22437/biospecies.v12i1.6596 ◽

2019 ◽

Vol 12 (1) ◽

pp. 49-54 ◽

Cited By ~ 1

Author(s):

Zulkarnain Z

Keyword(s):

Pollen Tube ◽

Pollen Germination ◽

Proper Time ◽

Sucrose Concentration ◽

Germination Rate ◽

Pollen Viability ◽

Pollen Grains ◽

Tube Formation ◽

Viability Assessment

The work presented here was aimed at investigating the proper time (after spreading) for viability assessment and the effect of various sucrose concentrations on pollen germination in Swainsona formosa. The rate of pollen tube formation was determined for freshly shed pollen grains of glasshouse-grown plants at 10, 15, 20, 25, 30, 60 and 120-minute intervals after being plated on Brewbaker and Kwack (BK) medium. The results indicated that within 60 minutes pollen germination reached 63.70%, after which remained steady at 120 minutes (63.71%). Under the effect of various concentrations of sucrose, i.e. 0.5, 10, 15, and 20% (w/v), the germination rate of pollen grains was assessed at 60 minutes following germination. The results showed that sucrose concentration of 10 – 15% (w/v) produced better germination rate (64.14%) compare to lower concentrations (19.64 and 43.58% at zero and 5% sucrose, respectively). Sucrose concentration above 15% was also found to inhibit pollen germination (48.92% at sucrose concentration of 20%).

Download Full-text

IN VITRO GERMINATION AND POLLEN TUBE GROWTH OF MAIZE (ZEA MAYS L.) POLLEN.: I. CALCIUM AND BORON EFFECTS

Canadian Journal of Botany ◽

10.1139/b67-087 ◽

1967 ◽

Vol 45 (6) ◽

pp. 839-845 ◽

Cited By ~ 46

Author(s):

P. L. Pfahler

Keyword(s):

Pollen Tube ◽

Boric Acid ◽

Calcium Nitrate ◽

Pollen Grains ◽

Germination Percentage ◽

Tube Length ◽

Artificial Medium ◽

Bacto Agar ◽

Pollen Tube Length

Pollen grains from the single cross hybrid Wf9 × H55 were cultured on an artificial medium containing 15% sucrose and 0.6% bacto-agar supplemented with all possible combinations of three concentrations (0.00, 0.03, and 0.06%) of calcium nitrate and three concentrations (0.00, 0.01, and 0.02%) of boric acid. The combination containing 0.03% calcium nitrate and 0.01% boric acid produced the highest germination (47.9%) and the longest tube length (405 μ). The addition of either calcium nitrate or boric acid alone decreased the germination percentage and pollen tube length. The percentage of grains producing more than one pollen tube was reduced by the addition of calcium nitrate or boric acid singly or in combination. The significance of these results in relation to pollen physiology was discussed.

Download Full-text

Factors to affect inbred beet plants while developing material for linear selection

Vavilov Journal of Genetics and Breeding ◽

10.18699/vj19.512 ◽

2019 ◽

Vol 23 (4) ◽

pp. 439-447 ◽

Cited By ~ 1

Author(s):

M. I. Fedorova ◽

E. G. Kozar ◽

S. A. Vetrova ◽

V. A. Zayachkovskyi ◽

V. A. Stepanov

Keyword(s):

Pollen Tube ◽

Recurrent Selection ◽

Germination Rate ◽

Pollen Viability ◽

Tube Length ◽

Self Incompatibility ◽

Seed Productivity ◽

The Third ◽

Male Gametes

Considering its capacities, the generative system of Beta vulgaris L. is regarded as highly productive. While inbreeding, the reproductive potential of cross-pollinated beet plants with gametophytic self-incompatibility (SI) changes significantly and is determined by a joint effect of multiple factors including the level of inbred depression. In the present study, original data have been obtained revealing relationships between inbred beet seed productivity, its self-incompatibility and microgametophyte parameters, which is crucial for developing and maintaining constant fertile beet lines. It has been discovered that inbred depression increases the number of sterile microgametes and anomalous pollen grains, reduces pollen fertility and the length of pollen tubes. As a result, the seed yield in inbred beet progeny, including SI ones, reduces significantly just after the third inbreeding. At the same time, highly productive inbred beet is characterized by a lower rate of pollen tube growth in vitro. In inbred plants, there is no close relationship between pollen viability and seed productivity, because the elimination of germinated male gametes and degeneration of seed embryos may go over the entire period of fertilization starting its progamic phase. The SI plants have more degenerating embryos than self-fertile ones, but seed vessel outgrowth in the seeds with abortive embryos makes them morphologically similar to fertile seeds. For that reason, when assessing inbred beet plants based on their self-incompatibility/self-fertility, one should consider the qualitative characteristics of the seeds. Using the method of recurrent selection based on such factors as seed productivity, pollen tube length and field germination rate increase the output of plant forms with a potentially high self-compatibility in their progeny. To support such genotypes in the progeny, one has to, starting from the third inbreeding, perform sib crossing to reduce the negative effect of inbred depression and self-incompatibility.

Download Full-text