scholarly journals In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

2017 ◽  
Vol 32 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Natalia Georgieva ◽  
Ivelina Nikolova ◽  
Valentin Kosev ◽  
Yordanka Naydenova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
In Vitro Germination ◽  
Pisum Sativum L ◽  
Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

scholarly journals In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

2013 ◽  
Vol 35 (4) ◽  
pp. 1116-1126 ◽  
Author(s):  
Taliane Leila Soares ◽  
Onildo Nunes de Jesus ◽  
Janay Almeida dos Santos-Serejo ◽  
Eder Jorge de Oliveira
Keyword(s):  
Pollen Germination ◽  
Culture Medium ◽  
Pollen Viability ◽  
Pollen Grains ◽  
In Vitro Germination ◽  
Triphenyltetrazolium Chloride ◽  
Histochemical Analysis ◽  
High Viability ◽  
Three Stages

The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro.

scholarly journals Determination of cardinal temperatures for sugar apple ( Annona squamosa L.)

2016 ◽  
Vol 40 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Bruno Rafael Alves Rodrigues ◽  
Rayane Carneiro dos Santos ◽  
Silvia Nietsche ◽  
Maria Olívia Mercadante-Simões ◽  
Isabella Renata Gomes da Cunha ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
In Vitro Germination ◽  
Cardinal Temperatures ◽  
Germination Of Pollen

ABSTRACT The sugar apple is highly dependent on the pollination and fruit tree pollen performance is crucial for successful fertilization and fruit set. The objective of this study was to determine the cardinal temperatures for in vitro pollen grains germination and evaluate the effect of temperature on pollen tube growth of sugar apple. The experimental design was in a completely randomized with eight treatments (10, 15, 20, 25, 30, 35, 40 and 45 ºC), four replicates and each plot was constituted by two slides per parcel. The cardinal temperatures were determined by evaluating in vitro germination of pollen grains and pollen tube growth in standard culture medium. It also assessed the pollen tube growth and the percentage of germination in vitro depending on the type of pollen grain arrangement (monad, dyad, triad and tetrad). In vitro germination of pollen grains and pollen tube growth varied significantly with temperature. The maximum germination recorded (48.13%) and the maximum lengths of pollen tubes (536.45 μm) were obtained when pollen grains were cultivated at 25 ºC. The estimated cardinal temperatures were 9.7, 26.9 and 44.2 ºC. Among the pollen grain arrangements, tetrads pollen grains were observed in higher proportions, however, monads pollen grains presented higher germination percentage.

scholarly journals Pollen tube growth of Paeonia tenuifolia L. (Paeoniaceae) in vitro and in vivo

1970 ◽  
Vol 40 (1) ◽  
pp. 93-95 ◽  
Author(s):  
Feruzan Dane ◽  
Nuran Ekici
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Tubes ◽  
In Vivo Studies ◽  
In Vivo Growth ◽  
Paeonia Tenuifolia

In vitro and in vivo studies on pollen germination of Paeonia tenuifolia L. (Paeoniaceae) revealed that pollen grains are shed at two-celled stage. Normal and abnormal pollens were observed. Pollen viability was recorded between 55 and 75%. In vitro studies revealed 85% germination and usually the germination was monosphonic. Some pollen tubes with swollen tube tip and undulations were found. Styles and stigma were found to contain many pollen tubes 24 hrs after pollination. Key words: Paeonia tenuifolia; Pollen tube; In vitro growth; In vivo growth  DOI: http://dx.doi.org/10.3329/bjb.v40i1.8003 Bangladesh J. Bot. 40(1): 93-95, 2011 (June)

scholarly journals Impact of Manganese on Pollen Germination and Tube Growth in Lily

2021 ◽  
Vol 74 ◽  
Author(s):  
Thomas Sawidis ◽  
Gülriz Baycu ◽  
Elżbieta Weryszko-Chmielewska ◽  
Aneta Sulborska
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Surrounding Medium ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Tube Growth ◽  
Low Concentrations ◽  
Main Effect

Abstract In vitro culture of Lilium longiflorum pollen grains was carried out to determine the role of manganese in pollen germination and pollen tube growth. Pollen germination was adversely affected by the presence of manganese (>10 −8 M), whereas low concentrations (10 −12 –10 −10 M) stimulated the process. Manganese caused morphological anomalies during tube growth, characterized by irregular pollen tube thickening and swollen tips. The main effect was the anomalous cell wall formation at the tip, in which the presence of several organelles reduced the number of secretory vesicles. A loose network of fibrillar material and spherical aggregates, mostly in the tip region, was detected, and this material was progressively loosened into the surrounding medium. As a response to potential toxicity, the excess manganese was isolated in vacuoles, which formed an internal barrier against penetration of manganese to the tip area. Elevated manganese concentrations might affect plant reproduction, resulting in anomalies in gamete development. Consequently, the loss in genetic diversity and decreased fruit set ultimately lower yield.

scholarly journals Foliar Application of Boron to Almond Trees Affects Pollen Quality

2000 ◽  
Vol 125 (2) ◽  
pp. 265-270 ◽  
Author(s):  
A.M.S. Nyomora ◽  
P.H. Brown ◽  
K. Pinney ◽  
V.S. Polito
Keyword(s):  
Pollen Germination ◽  
Foliar Application ◽  
Culture Media ◽  
Pollen Viability ◽  
Prunus Dulcis ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Mature Trees

The effect of boron (B) on in vivo and in vitro development of almond [Prunus dulcis (Mill.) D.A. Webb (syn. P. amygdalus Batsch)] pollen and pollen tubes and the resultant effect on fruit set was studied in mature trees. The cultivars Mono (pistil donor) and Butte (pollinizer) in an orchard with low soil B in Fresno, California were sprayed with B at 0, 0.8, 1.7, or 2.5 kg·ha-1 during Fall 1993. Pollen viability as indicated by the fluorescein diacetate method (FDA) was >85% and was not affected by field-applied B, however, in vivo pollen germination and tube growth were enhanced by foliar-applied B. More effect of applied B on in vivo growth appeared as pollen tubes progressed toward the ovary. For in vitro germination, foliar-applied B reduced bursting of tubes, and addition of B to the culture media significantly increased pollen germination and pollen tube growth.

scholarly journals Effect of high temperature on the reproductive development of chickpea genotypes under controlled environments

2012 ◽  
Vol 39 (12) ◽  
pp. 1009 ◽  
Author(s):  
Viola Devasirvatham ◽  
Pooran M. Gaur ◽  
Nalini Mallikarjuna ◽  
Raju N. Tokachichu ◽  
Richard M. Trethowan ◽  
...  
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Pollen Grains ◽  
High Temperature Stress ◽  
Pollen Production ◽  
Seed Number ◽  
Controlled Environments

High temperature during the reproductive stage in chickpea (Cicer arietinum L.) is a major cause of yield loss. The objective of this research was to determine whether that variation can be explained by differences in anther and pollen development under heat stress: the effect of high temperature during the pre- and post-anthesis periods on pollen viability, pollen germination in a medium, pollen germination on the stigma, pollen tube growth and pod set in a heat-tolerant (ICCV 92944) and a heat-sensitive (ICC 5912) genotype was studied. The plants were evaluated under heat stress and non-heat stress conditions in controlled environments. High temperature stress (29/16°C to 40/25°C) was gradually applied at flowering to study pollen viability and stigma receptivity including flower production, pod set and seed number. This was compared with a non-stress treatment (27/16°C). The high temperatures reduced pod set by reducing pollen viability and pollen production per flower. The ICCV 92944 pollen was viable at 35/20°C (41% fertile) and at 40/25°C (13% fertile), whereas ICC 5912 pollen was completely sterile at 35/20°C with no in vitro germination and no germination on the stigma. However, the stigma of ICC 5912 remained receptive at 35/20°C and non-stressed pollen (27/16°C) germinated on it during reciprocal crossing. These data indicate that pollen grains were more sensitive to high temperature than the stigma in chickpea. High temperature also reduced pollen production per flower, % pollen germination, pod set and seed number.

scholarly journals In VitroPollen Viability and Pollen Germination in Cherry Laurel (Prunus laurocerasusL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Melekber Sulusoglu ◽  
Aysun Cavusoglu
Keyword(s):  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Second Step ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Germinated Pollen ◽  
Cherry Laurel ◽  
Better Than

Pollen quality is important for growers and breeders. This study was carried out to determinein vitropollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasusL.). Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (iodine potassium iodide), were used. Pollen traits of genotypes were studied using anin vitromedium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r2= 0.0614 andr2= 0.0015, resp.). Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

Viability and in Vitro Germination of Johnsongrass (Sorghum Halepense) Pollen

2007 ◽  
Vol 21 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Ian C. Burke ◽  
John W. Wilcut ◽  
Nina S. Allen
Keyword(s):  
Suspension Culture ◽  
Boric Acid ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Calcium Nitrate ◽  
Medium Composition ◽  
In Vitro Tests ◽  
In Vitro Germination ◽  
Agar Culture

A high proportion of viable pollen grains must germinate to study the physiology of pollen growth to reduce the confounding effects of environmental influences on pollen germination. The objectives of this study were to evaluate the nuclear state and develop a suitable medium and culture method for in vitro germination of johnsongrass pollen. Johnsongrass pollen was trinucleate, and in vitro tests for pollen viability using Alexander's stain and a fluorochromatic reaction method (FCR) indicated johnsongrass pollen was viable (92.6 to 98.4%). A factorial treatment arrangement of four concentrations of sucrose, two concentrations of boric acid, and two concentrations of calcium nitrate were used to determine the optimum pollen-germination medium composition in suspension culture, agar culture, and cellophane membrane culture. Germination was highest in a suspension culture with a medium containing 0.3 M sucrose, 2.4 mM boric acid, and 3 mM calcium nitrate. Pollen germination using this medium was 78.9% when anthers were harvested just before anthesis.

scholarly journals Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa

Silva Fennica ◽  
2019 ◽  
Vol 53 (2) ◽  
Author(s):  
Yan Liu ◽  
Yuan Zhang ◽  
Qing Zhou ◽  
Jian Wu ◽  
Pingdong Zhang
Keyword(s):  
Pollen Germination ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Populus Tomentosa ◽  
2N Pollen ◽  
Significant Difference ◽  
Diploid Pollen ◽  
Germination Experiments ◽  
Diploid Gametes

Colchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, followed by pollen germination and crossing induced pollen with normal gametes to produce triploid in Carrière. The results showed that the predominant meiotic stages and the number of colchicine injections had significant effects on the occurrence rates of induced 2n pollen. When the colchicine injection was given at diakinesis, a significant decrease in the pollen production per bud was observed ( < 0.001). The morphology of the colchicine-induced 2n pollen was similar to that of the natural 2n pollen in its ectexine structure. The pollen germination experiments revealed that there was also no significant difference in germination rates between the induced diploid pollen and natural 2n pollen grains, and 68 triploids were created by crossing colchicine-induced pollen. Our findings revealed that colchicine injection could induce to produce 2n pollen and will not lead to dysfunction of induced diploid pollen.in vitroPopulus tomentosapP. tomentosa

scholarly journals Technique for In Vitro Pollen Germination and Short-term Pollen Storage in Caladium

HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 365-367 ◽  
Author(s):  
Zhanao Deng ◽  
Brent K. Harbaugh
Keyword(s):  
Pollen Germination ◽  
Large Scale ◽  
Pollen Viability ◽  
Storage Conditions ◽  
In Vitro Germination ◽  
Short Term ◽  
Pollen Storage ◽  
Breeding Programs ◽  
Sucrose Level

The sporadic nature of inflorescence production and flower protogyny in caladium (Caladium ×hortulanum Birdsey) makes it desirable to store pollen and to rapidly assess its viability for cross-pollinations in breeding programs. This study was conducted to develop a procedure to determine caladium pollen viability and to use that procedure to evaluate the effect of short-term storage conditions on pollen viability. The sucrose level in the culture medium was found to have a significant impact on the in vitro germination of caladium pollen; a concentration of 6.8% was determined to be optimal for pollen germination. Caladium pollen lost viability within 1 day under room (24 °C) or freezing (-20 °C) temperatures, but could be stored at 4 °C for 2 to 4 days. Pollen stored at 4 °C produced successful pollinations. Data obtained from large-scale greenhouse pollinations supported use of this in vitro germination assay as a convenient way to evaluate caladium pollen viability (and fertility).

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