In vitro propagation of Epacris impressa (Epacridaceae) and the effects of clonal material

A system of micropropagation has been developed for Epacris impressa Labill. (pink heath) (Epacridaceae), the floral emblem of Victoria, Australia. Only explants from glasshouse-grown plants treated with 1.2 g L–1 mancozeb were established successfully in vitro. Shoot material was very sensitive to surface-sterilisation, with 0.5% NaOCl for 5 min being optimal. Multiple shooting was induced optimally on Woody Plant Medium (WPM, Lloyd and McCown 1980) with 12–25 µM of the cytokinin 2iP (6-(γ,γ-dimethylallylamino) purine). Inclusion of the auxin IBA (indole-3-butyric acid) induced callus and reduced shooting. Rooting in vitro was greatest (up to 40%) with half-strength WPM and 16 µM IBA. Clones from individual plants varied in multiple shooting response to 2iP (0–49 µM) and root induction response to auxins (IBA and NAA (α-naphthaleneacetic acid), 0–43 µM). These results suggest that explant materials are the main determinant of success in in vitro propagation and that they require individual optimisation of treatments to maximise shoot and root formation.

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Micropropagation of Antelope Bitterbrush [Purshia tridentata (Pursh) DC.]

HortScience ◽

10.21273/hortsci.32.2.312 ◽

1997 ◽

Vol 32 (2) ◽

pp. 312-314 ◽

Cited By ~ 1

Author(s):

John L. Edson ◽

David L. Wenny ◽

Annette Leege-Brusven

Keyword(s):

Woody Plant ◽

Naphthaleneacetic Acid ◽

Ex Vitro ◽

Indolebutyric Acid ◽

Axillary Shoots ◽

Full Strength ◽

Woody Plant Medium ◽

Half Strength ◽

In Vitro Survival

In vitro—derived microshoots of antelope bitterbrush, incubated for 1 month in media supplemented with 0.44 μm BA, grew 0.8 and 1.1 cm longer in woody plant medium (WPM) compared to full-strength and half-strength Murashige and Skoog (MS) media, respectively. Explants cultured in WPM supplemented with 0.44 μm BA and 0.54 μm NAA produced a mean of five axillary shoots per explant. Explants dipped in 0.1% IBA or 0.1% NAA rooted best in 0.1% IBA with 89% success ex vitro vs. 60% success in vitro. Survival of acclimatized plantlets rooted ex vitro was 95%, while 50% survived when rooted in vitro. After 1 year of greenhouse growth, 98% of plantlets survived and flowered. Chemical names used: benzyladenine (BA), 3-indolebutyric acid (IBA), 1-naphthaleneacetic acid (NAA).

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In vitro propagation of Xao tam phan (Paramignya trimera (Oliv.) Guill.)

Science and Technology Development Journal - Natural Sciences ◽

10.32508/stdjns.v1it2.452 ◽

2017 ◽

Vol 1 (T2) ◽

pp. 48-57

Author(s):

Hieu Trung Tran ◽

Chung Van Huynh ◽

Hue Thi Linh Bui ◽

Ngan Thi My Luong ◽

Anh Lan Bui ◽

...

Keyword(s):

In Vitro Propagation ◽

Word Of Mouth ◽

Woody Plant ◽

Leaf Explants ◽

Anticancer Properties ◽

Best Response ◽

Woody Plant Medium ◽

Old Trees ◽

Cut Surface

Paramignya trimera (Oliv.) Guill., a woody climber commonly known as "Xao tam phan", has been used in Vietnamese folk for the treatment of numerous cancers. Due to word of mouth about the anticancer properties of this plant, its stems and roots have been overexploited leading to the serious decline of this species in Phu Yen, Khanh Hoa and Ninh Thuan provinces. The aim of the study was to establish an in vitro propagation protocol for the conservation of P. trimera. In this research shoot clusters (5–8 shoots/cluster) were regenerated from axillary bud explants of 1–3 year-old trees after 3 months of cultures on the WPM (woody plant medium) supplemented with STS 3 and BA 5–7 mg/L. STS (silver thiosulfate) was used to prevent the leaf abscission. These shoot clusters grew slowly and reached 1–3 cm in heights after 4 months of the cultures. These shoot clusters did not form any roots after 2 months of culture on the rooting media with IBA and/or NAA 1–5 mg/L. However, there was 51 % of the treated shoot clusters acclimatized and produced new stem and leaves after 2 months growing in greenhouse. WPM supplemented with STS 3, BA 5 and IBA 5 mg/L showed the best response for callus induction in leaf explants after 3 months of cultures. Among the callus types, the milky white compact calli were induced at the cut surface of leaf explants after 3 months of the cultures and became the compact and nodulated calli within 4 weeks later.

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Microspore embryogenesis in anther culture of three species of Populus and regeneration of dihaploid plants of Populustrichocarpa

Canadian Journal of Forest Research ◽

10.1139/x93-231 ◽

1993 ◽

Vol 23 (9) ◽

pp. 1821-1825 ◽

Cited By ~ 20

Author(s):

Snorri Baldursson ◽

Peter Krogstrup ◽

Jens Viktor Nørgaard ◽

Sven Bode Andersen

Keyword(s):

Chromosome Number ◽

Growth Regulator ◽

Woody Plant ◽

Adventitious Shoots ◽

Microspore Embryogenesis ◽

Naphthaleneacetic Acid ◽

Chromosome Counts ◽

Low Frequencies ◽

Woody Plant Medium

Microspore embryogenesis was induced from in vitro cultured anthers of Populusbalsamifera L., Populusmaximowiczii A. Henry, and Populustrichocarpa Torr. & Gray. Embryoids were formed at low frequencies on a modified Murashige and Skoog's medium, supplemented with 5 μM 6-benzylaminopurine, 5.1 mM L-glutamine, and 6% maltose. Growth regulator combinations (0–10 μM 6-benzylaminopurine and naphthaleneacetic acid) affected embryogenesis only slightly but formation of nonembryogenic callus from the anthers increased with increasing concentration of naphthaleneacetic acid. One donor clone of P. trichocarpa produced 54 embryoids from as many anthers during the two years of study. Adventitious shoots were obtained from 31 of these embryoids on woody plant medium with 2.5 μM 6-benzylaminopurine and 0.005 μM naphthaleneacetic acid. Adventitious shoots from 25 different embryoids were successfully rooted on woody plant medium containing 0.25 μM indole-3-butyric acid and transplanted to soil. Isozyme analysis confirmed microspore origin of all plants studied, and chromosome counts revealed that most of them had doubled their chromosome number spontaneously.

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(175) In Vitro Regeneration of Cladrastis kentukea

HortScience ◽

10.21273/hortsci.40.4.1052c ◽

2005 ◽

Vol 40 (4) ◽

pp. 1052C-1052

Author(s):

Denita Hadziabdic ◽

Robert N. Trigiano ◽

Stephen Garton ◽

Mark T. Windham ◽

William E. Klingeman

Keyword(s):

Growth Regulators ◽

Root Length ◽

Woody Plant ◽

In Vitro Regeneration ◽

Cambial Activity ◽

Ms Medium ◽

Total Root Length ◽

Woody Plant Medium ◽

Half Strength

Axillary buds from a single Cladrastis kentukea tree were initially cultured on two media, woody plant medium (WPM) and Murashige and Skoog (MS) containing 0, 1, 2, or 4 μm 6–benzylaminopurine (BA). Cultures were transferred to fresh media every 4 weeks. Elongated shoots were harvested after 39 weeks and transferred to half-strength MS medium supplemented with the following concentrations of IBA: 0, 3, 30, 100, and 300 μm for 3 d, then returned to half-strength MS without growth regulators. Explants exposed to 300 μm of IBA produced significantly more roots (75%) compared to explants exposed to other treatments. Fifty-four and 45% of the microshoots rooted when exposed to 100 and 30 μm IBA, respectively. Only 4% of the microshoots rooted when exposed to 3 μm IBA and none of the control microshoots rooted. Although the 300 μm treatment yielded the most rooted plantlets, there was significantly higher terminal meristem abortion compared to other treatments. There were no statistical differences between the numbers of roots and total root length among all treatments. Additionally, all microshoots that rooted had lenticels, suggesting that presence of lenticel cambial activity can possibly improve rooting abilities of selected microshoots. Rooted microshoots were gradually acclimatized to nonsterile environment.

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(174) In Vitro Regeneration of Cornus kousa

HortScience ◽

10.21273/hortsci.40.4.1052b ◽

2005 ◽

Vol 40 (4) ◽

pp. 1052B-1052

Author(s):

Denita Hadziabdic ◽

Robert N. Trigiano ◽

Stephen Garton ◽

Mark T. Windham

Keyword(s):

Woody Plant ◽

In Vitro Regeneration ◽

Root Production ◽

Naphthaleneacetic Acid ◽

Apical Buds ◽

Cornus Kousa ◽

Woody Plant Medium ◽

Rooting Media ◽

Indole 3 Acetic Acid

Axillary and apical buds from five Cornus kousa cultivars (`Little Beauty', `Samaritan', `Heart Throb', `Rosabella', and `Christian Prince') were initially established on two basal media, woody plant medium (WPM) and woody plant medium/broad leaved tree medium (BW), amended with the following concentrations of 6–benzylaminopurine (BA): 0, 2, 4, and 8 μm. After explants were transferred at 4-week intervals for 28 weeks beginning in April, only microshoots of `Samaritan', `HeartThrob' and `Rosabella', were harvested from proliferating cultures and placed on rooting media. `Little Beauty' and `Christian Prince' did not perform well in multiplication phase of tissue culture and were excluded from further studies. Rooting media contained WPM or BW supplemented with either 1-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) at the following concentrations: 0, 0.5, 1.5, 4.5, and 13.5 μm. Six weeks following rooting experiment, preliminary data were collected and results showed that total of nine plants rooted on both WPM and BW media supplemented with IBA, 17 plants rooted on media supplemented with NAA, and 14 plants rooted on media supplemented with IAA. These results indicated that NAA and IAA appeared to be better for root production on C. kousa cultivar microshoots than IBA. Additionally, WPM supported more root production, when compared to BW, even though both media resulted in rooted microshoots. Proliferating masses were placed on fresh medium with 2μm BA and were used again for the rooting projects.

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In vitro Propagation of Globba schomburgkii Hook. f. via Bulbil Explants

Walailak Journal of Science and Technology (WJST) ◽

10.48048/wjst.2018.3507 ◽

2017 ◽

Vol 15 (10) ◽

pp. 701-710

Author(s):

Piyaporn SAENSOUK ◽

Surapon SAENSOUK ◽

Phattaraporn PIMMUEN

Keyword(s):

Plant Regeneration ◽

Callus Induction ◽

In Vitro Propagation ◽

Root Formation ◽

Survival Rates ◽

Shoot Formation ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Different Types

An efficient and rapid protocol for the micropropagation of Globba schomburgkii Hook. f. via bulbil explants was investigated. The long divided and undivided bubils of G. schomburgkii Hook. f. were cultured on MS medium (Murashige and Skoog) that had either 3 mg/l benzyladenine (BA) or 0.5 mg/l naphthaleneacetic acid (NAA) added for 8 weeks. The results indicated that the long divided bulbils of G. schomburgkii Hook. f. showed a greater amount of plant regeneration than the undivided bulbils. Callus induction, as well as shoot and root formation, were observed when culturing microshoots of 1 cm in length on media (MS) that had Thidiazuron (TDZ) or NAA plus BA added at a range of concentrations for 8 weeks. The highest percentage of callus induction was 40 % when culturing the microshoots on MS medium supplemented with NAA and BA. The best result for shoot formation was achieved when culturing the microshoots on MS medium with TDZ added. The highest number of roots was obtained when culturing the microshoots on MS medium with NAA and BA added. The in vitro-derived plantlets of G. schomburgkii Hook. f. were transplanted to pots containing different types of potting mixture in a greenhouse. The survival rates were 80 % when G. schomburgkii Hook. f. was transplanted to sand.

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Micropropagation of two threatened Tasmanian species of Calocephalus (Asteraceae), with comments on phenotypic plasticity

Australian Journal of Botany ◽

10.1071/bt02120 ◽

2003 ◽

Vol 51 (4) ◽

pp. 415 ◽

Cited By ~ 2

Author(s):

Rebecca Jane Sands ◽

Natalie Ruth Brown ◽

Anthony Koutoulis

Keyword(s):

Phenotypic Plasticity ◽

Plant Growth Regulator ◽

Root Formation ◽

Indoleacetic Acid ◽

Naphthaleneacetic Acid ◽

Root Induction ◽

Ms Medium ◽

Ex Vitro ◽

Morphological Differences

Micropropagation systems were developed for Calocephalus citreus Less. and C. lacteus Less., two threatened Tasmanian members of the Asteraceae. Disinfected cold-treated capitula were used to initiate regeneration. For C. citreus, initiation was achieved on Murashige and Skoog (MS) medium with 0.1�mg�L–1 or 0.5�mg�L–1 indoleacetic acid (IAA) and 1�mg�L–1 6-benzylaminopurine (BAP) in 5�weeks, while for C. lacteus initiation was achieved on MS with α-naphthaleneacetic acid (NAA) (0.1�mg�L–1) in 3�weeks and on MS without any plant growth regulator (PGR) in 6�weeks. Multiplication was achieved in both species on MS with various concentrations of IAA (0.01–0.5�mg�L–1) and BAP (0.1–1�mg�L–1). In C. citreus, shooting in all treatments did not differ significantly from PGR-free MS, while in C. lacteus PGR-free MS was one of the better treatments. Multiplication media also initiated root formation in C. lacteus, thereby facilitating immediate planting out. Optimal root induction in C. citreus was achieved by using MS with 1�g�L–1 activated charcoal. Clear morphological differences between in vitro and ex vitro plants of both species were observed. This phenotypic plasticity was more pronounced in C. lacteus than in C. citreus. As C. lacteus has a wider distribution than C. citreus and C. lacteus was more responsive during many stages of the micropropagation process, it may be possible to use the culture-induced phenotype to provide insights into the ecology of plant species.

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Propagación in vitro de Psidium guajaba mediante organogénesis directa a partir de segmentos nodales

Ciencia y Tecnología Agropecuaria ◽

10.21930/rcta.vol8_num1_art:79 ◽

2007 ◽

Vol 8 (1) ◽

pp. 22 ◽

Cited By ~ 1

Author(s):

Fabiola Ocampo ◽

Víctor Manuel Núñez

Keyword(s):

In Vitro Propagation ◽

Woody Plant ◽

Adventitious Shoots ◽

Nodal Segments ◽

Direct Organogenesis ◽

Post Inoculation ◽

Ex Vitro ◽

Mass Multiplication ◽

Woody Plant Medium

Se indujeron múltiples brotes mediante organogénesis directa a partir de segmentos nodales de 10 genotipos diferentes de guayaba. Para ello se estableció un sistema de propagación clonal in vitro combinado con inducción rápida de brotes ex vitro para propagar árboles élite. La utilización de segmentos nodales permitió obtener en poco tiempo brotes adventicios adecuados para multiplicación masiva. La respuesta in vitro de los genotipos fue evaluada usando los medios de cultivo MS (Murashige y Skoog, 1962), Mc (Mascarenhas) y WPM (Woody Plant Medium) suplementados con 0,1 mg•L-1 de ácido indolácetico (AIA) y 0,25 mg•L-1 de bencilaminopurina (BAP). El procedimiento de desinfección con hipoclorito de sodio previno eficientemente la contaminación de los explantes después de la inoculación en el medio de cultivo. El mayor porcentaje en la inducción de brotes se logró con 0,25 mg•L-1 de BAP. Los segmentos nodales presentaron de 1 a 2 brotes adventicios por explante después de 15 días de inoculados y de 3 a 7 brotes a los 30 días después del inicio del cultivo. Una vez individualizados los brotes se usaron en una nueva fase de multiplicación masiva en la que se probaron cuatro sustratos diferentes durante el enraizamiento y el endurecimiento. Esta metodología permitió la propagación in vitro de guayaba cuatro semanas después del inicio del cultivo. Los mejores resultados se lograron con el medio WPM que permitió obtener las primeras plántulas enraizadas dos semanas después de la transferencia al sustrato de enraizamiento. In vitro propagation of Psidium guajaba using direct organogenesis from nodal segmentsMultiple shoots were induced using direct organogenesis from nodal segments of 10 different genotypes of guayaba. For this an in vitro clonal propagation system combined with rapid ex vitro induction of shoots was established in order to propagate elite trees. The use of nodal segments resulted in adventitious shoots adequate for mass multiplication in less time. The in vitro response of the genotypes was evaluated using the culture media MS (Murashige and Skoog, 1962), Mc (Mascarenhas) and WPM (Woody Plant Medium) supplemented with 0.1 mg·L-1 of indole acetic acid (IAA) and 0.25 mg·L-1 of benzoaminopurine (BAP). The procedure for sterilizing with sodium hypochlorite effectively prevented the contamination of the explants after the inoculation of the culture medium. The greatest percentage of shoot induction was achieved with 0.25 mg·L-1 of BAP. The nodal segments showed between 1-2 adventitious shoots per explant 15 days post-inoculation and 3-7 shoots 30 days post-inoculation. Once individualized, the shoots were used in a new mass multiplication phase in which four different substrates were tested during rooting and hardening. This methodology permitted the in vitro propagation of guayaba four weeks post-inoculation. The best results were achieved with the WPM medium that resulted in the first rooted plantlets two weeks after the transfer to the rooting substrate.

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Micropropagation of Strawberry (Fragaria ananassa) through runner culture

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v38i3.16973 ◽

2013 ◽

Vol 38 (3) ◽

pp. 467-472 ◽

Cited By ~ 3

Author(s):

M Ashrafuzzaman ◽

SM Faisal ◽

D Yadav ◽

D Khanam ◽

F Raihan

Keyword(s):

In Vitro Propagation ◽

Average Length ◽

Root Induction ◽

Fragaria Ananassa ◽

Shoot Induction ◽

Half Strength ◽

Number Of Leaves ◽

Number Of Shoots

In vitro propagation of strawberry was conducted at the Biotechnology Lab. of BARI, Joydebpur, Gazipur. For shoot induction, five BAP concentrations viz., 0.0 (Control), 0.5, 1.0, 1.5, and 2.0 mg/l and for root induction four IBA concentrations viz., 0.0 (Control), 0.5, 1.0, and 1.5 mg/l were used. The highest average number of shoots (7) and the highest average length (3.34 cm) of shoot was observed at the concentration of 0.5 mg/l BAP. The highest average number of leaves (5) was also observed at the same concentration. Among the five rooting concentrations, IBA @ 0.5 mg/l showed the best performance in all the parameters studied. The highest number (6) of roots/culture and the longest (3.05 cm) roots were also obtained from this concentration. Half strength MS media without IBA concentration did not show any response regarding root induction. DOI: http://dx.doi.org/10.3329/bjar.v38i3.16973 Bangladesh J. Agril. Res. 38(3): 467-472, September 2013

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Impact of Indole-3-Butyric Acid (IBA) on the Root Induction of Arbutus pavarii Pamp (Lybian Strawberry Tree) in in vitro Culture

Asian Journal of Research in Agriculture and Forestry ◽

10.9734/ajraf/2019/v4i330062 ◽

2019 ◽

pp. 1-6

Author(s):

Hameda A. Yousef ◽

Hanan A. Idris ◽

Rabha M. Mansur

Keyword(s):

Butyric Acid ◽

Plant Growth Regulator ◽

In Vitro Propagation ◽

Dry Weight ◽

Induction Medium ◽

Root Induction ◽

Endangered Plant ◽

Half Strength ◽

Growth Indicators

The main objective of this study was to clarify the best concentration of the indole-3-butyric acid (IBA) in order to induce the formation of strong roots of Arbutus pavarii Pamp, an endangered plant in the El-Jabel El-Akhdar region in Libya. A study was carried out to find a protocol for its in vitro propagation. The present paper aimed to investigate the effects of different concentrations of IBA plant growth regulator on the rooting. Three weeks old seedlings obtained with in vitro germination were transferred to Murashige and Skoog (M&S) roots induction medium supplemented with different concentrations of IBA (0, 1, 1.5 and 2 mg L-1). The highest response was obtained with the M&S medium half-strength supplemented with IBA 1 mg L-1 concentration. All the measured growth indicators (rooting percentage, root length and dry weight) significantly enhanced when using this concentration.

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