scholarly journals Impact of Indole-3-Butyric Acid (IBA) on the Root Induction of Arbutus pavarii Pamp (Lybian Strawberry Tree) in in vitro Culture

2019 ◽  
pp. 1-6
Author(s):  
Hameda A. Yousef ◽  
Hanan A. Idris ◽  
Rabha M. Mansur
Keyword(s):  
Butyric Acid ◽  
Plant Growth Regulator ◽  
In Vitro Propagation ◽  
Dry Weight ◽  
Induction Medium ◽  
Root Induction ◽  
Endangered Plant ◽  
Half Strength ◽  
Growth Indicators

The main objective of this study was to clarify the best concentration of the indole-3-butyric acid (IBA) in order to induce the formation of strong roots of Arbutus pavarii Pamp, an endangered plant in the El-Jabel El-Akhdar region in Libya. A study was carried out to find a protocol for its in vitro propagation. The present paper aimed to investigate the effects of different concentrations of IBA plant growth regulator on the rooting. Three weeks old seedlings obtained with in vitro germination were transferred to Murashige and Skoog (M&S) roots induction medium supplemented with different concentrations of IBA (0, 1, 1.5 and 2 mg L-1). The highest response was obtained with the M&S medium half-strength supplemented with IBA 1 mg L-1 concentration. All the measured growth indicators (rooting percentage, root length and dry weight) significantly enhanced when using this concentration.

scholarly journals Effects of Some Antioxidants on in Vitro Rooting of Apple Shoots

HortScience ◽  
1990 ◽  
Vol 25 (11) ◽  
pp. 1435-1436 ◽  
Author(s):  
Alvaro Standardi ◽  
Fausto Romani
Keyword(s):  
Citric Acid ◽  
Root Elongation ◽  
Induction Medium ◽  
Root Induction ◽  
Root Initiation ◽  
Acid Sodium Salt ◽  
Fast Decline ◽  
Half Strength ◽  
Diethyldithiocarbamic Acid

In vitro-proliferated shoots of `Delicious' and `Starkspur Red' apples (Malus domestica Borkh.) were submitted for 1 week to root induction in the dark with their basal parts in liquid medium containing 1.5 μm IBA and 43.4 mm sucrose. The shoots were transferred to root initiation and root elongation medium made up of plugs wetted with half-strength Lepoivre salts. When antioxidants, such as PVP, GH, 2-Me, and DIECA, were added to the liquid induction medium, the percentages of rooting usually decreased. In contrast, if the antioxidants PVP and citric acid were added in the initiation-elongation medium, the percentages of rooting increased. The results agree with the finding of other researchers who proposed a rapid increase in peroxidase activity during root induction followed by a fast decline during rooting initiation as a marker for good rooting responses. Moreover, the use of antioxidants both in induction and in initiation-elongation had no effect on number and length of roots. Chemical names used: polyvinylpyrrolidone-40 (PVP); glutathione reduced (GH); 2-mercaptoethanol (2-Me); diethyldithiocarbamic acid, sodium salt trihydrate (DIECA); N-(phenylmethyl) 1 H-purin-6-amine (BA); 1 H-indole-3-butyric acid (IBA); gibberellic acid (GA3).

In vitro propagation of Epacris impressa (Epacridaceae) and the effects of clonal material

2000 ◽  
Vol 48 (2) ◽  
pp. 215 ◽  
Author(s):  
J. Anthony ◽  
C. B. McLean ◽  
A. C. Lawrie
Keyword(s):  
In Vitro Propagation ◽  
Root Formation ◽  
Woody Plant ◽  
Multiple Shooting ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Main Determinant ◽  
Woody Plant Medium ◽  
Half Strength

A system of micropropagation has been developed for Epacris impressa Labill. (pink heath) (Epacridaceae), the floral emblem of Victoria, Australia. Only explants from glasshouse-grown plants treated with 1.2 g L–1 mancozeb were established successfully in vitro. Shoot material was very sensitive to surface-sterilisation, with 0.5% NaOCl for 5 min being optimal. Multiple shooting was induced optimally on Woody Plant Medium (WPM, Lloyd and McCown 1980) with 12–25 µM of the cytokinin 2iP (6-(γ,γ-dimethylallylamino) purine). Inclusion of the auxin IBA (indole-3-butyric acid) induced callus and reduced shooting. Rooting in vitro was greatest (up to 40%) with half-strength WPM and 16 µM IBA. Clones from individual plants varied in multiple shooting response to 2iP (0–49 µM) and root induction response to auxins (IBA and NAA (α-naphthaleneacetic acid), 0–43 µM). These results suggest that explant materials are the main determinant of success in in vitro propagation and that they require individual optimisation of treatments to maximise shoot and root formation.

Histology of organogenesis and somatic embryogenesis in excised root cultures of an endangered species Tylophora indica (Asclepiadaceae)

2010 ◽  
Vol 58 (3) ◽  
pp. 198 ◽  
Author(s):  
Aastha Sahai ◽  
Anwar Shahzad ◽  
Shiwali Sharma
Keyword(s):  
Somatic Embryogenesis ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium ◽  
Tylophora Indica ◽  
Regenerated Plants ◽  
Maturation Medium ◽  
Half Strength ◽  
One Year

This paper reports an efficient regeneration protocol through parallel organogenic and embryogenic pathways from green root segments (GRSs) of Tylophora indica (Burm.f) Merrill. GRSs explants from one year old in vitro cultures were cultured on Murashige and Skoog (MS) medium containing various cytokinins. Five µmol/L of 6-benzyladenine (BA) was most responsive for organogenesis in 1.5 cm long GRSs. Repeated subculture on medium containing both BA (5 µmol/L) and 1-naphthleneacetic acid (NAA) (0.1 µmol/L) promoted multiplication and proliferation of direct shoot buds (46.80 ± 0.96) and callus mediated somatic embryogenesis (18.07 ± 0.33). Germinated embryos isolated from callus were transferred onto maturation medium consisting of half-strength MS medium either devoid of plant growth regulators (PGRs) or with various concentrations of gibberellic acid (GA). Microshoots were excised during subculture and transferred onto root induction medium, thus ensuring a continuous supply of germplasm. Morphogenic variations were noticed in types of roots induced on various auxins. Regenerated plantlets and emblings hardened best on vermiculite with a survival rate of 90% and 70% respectively. However, the emblings were healthier in comparison to the regenerated plants. Histological analysis showed the origin and development of organogenesis.

scholarly journals In vitro clonal multiplication of Aegle marmelos (L.) Corr. through cotylodonary node culture

2013 ◽  
Vol 48 (1) ◽  
pp. 13-18 ◽  
Author(s):  
S Akter ◽  
TA Banu ◽  
MA Habib ◽  
S Afrin ◽  
A Khatun ◽  
...  
Keyword(s):  
Multiple Shoots ◽  
Poor Response ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium ◽  
Aegle Marmelos ◽  
Clonal Multiplication ◽  
Cotyledonary Nodes ◽  
Half Strength

Clonal multiplication of Aegle marmelos was achieved through in vitro culture. Cotyledonary nodes from one month old in-vitro grown seedlings of Aegle marmelos were cultured on MS medium supplemented with BAP, Kn, and IBA either alone or in combination. The highest regenerative response was observed on medium containing 2.5 mg/L BAP and 0.5 mg/L Kn from node and shoot tip within 8-10 days. When regenerated shoots were subcultured on MS medium supplemented with 0.2 mg/L BAP and 0.02 mg/L Kn, maximum number of multiple shoots were observed after third phase of subculture. Poor response was found using MS medium supplemented with Kn only. In vitro induced shoots were transferred into root induction medium consisting of half-strength MS supplemented with auxins, IAA, IBA or NAA. Rooting was best in medium supplemented with 1.0 mg/L IBA. Rooted plantlets were acclimatized and transferred to the soil with 96% survival rate. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15408 Bangladesh J. Sci. Ind. Res. 48(1), 13-18, 2013

scholarly journals Cytokinin and auxin levels in micropropagating Red Fuji and McIntosh apple varieties

2001 ◽  
pp. 53-59
Author(s):  
Katalin Magyarné Tábori ◽  
Judit Dobránszki ◽  
Antal Ferenczy ◽  
Erzsébet Jámborné Benczúr ◽  
János Lazányi
Keyword(s):  
Butyric Acid ◽  
Activated Charcoal ◽  
Root Elongation ◽  
Shoot Multiplication ◽  
Induction Medium ◽  
Root Induction ◽  
Proliferation Medium ◽  
Effects Of Media ◽  
Rooting Capacity

Effects of media hormone content on in vitro shoot multiplication and rooting were examined in cv. Red Fuji and McIntosh apple scions. Multiplication responses of shoots to different concentrations (0.5 and 1.0 mg/l) of 6-benzylaminopurine and 6-benzylaminopurine riboside were tested at two levels (0.1 and 0.3 mg/l) of indole-3-butyric acid. The best proliferation rate was achieved on medium containing 1.0 mg/l 6-benzylaminopurine and 0.1 mg/l indole-3-butyric acid in cv. Red Fuji (5.3) and on medium containing 1.0 mg/l 6-benzylaminopurine and 0.3 mg/lindole-3-butyric acid in cv McIntosh (10.3). The length of shoots on these media was enough for rooting (38.4 mm in cv. Red Fuji and 39.3 mm in cv McIntosh). Shoots developed on the best proliferation medium were used for rooting. Effects of different concentrations of auxin in the root induction media and presence of activated charcoal in the root elongation media were examined on rooting capacity. The best rooting rate (100% in cv. McIntosh and 83% in cv. Red Fuji) was achieved when the root induction medium contained 1.0 mg/l indole-3-butyric acid. In general, rooting was inhibited in the presence of activated charcoal.  Because of high in vitro multiplication and rooting rate and high percent of survival during acclimatisation, the methods described here make effective micropropagation processes possible for cv. Red Fuji and McIntosh. 

scholarly journals Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

2016 ◽  
Vol 4 (1) ◽  
pp. 61-69 ◽  
Author(s):  
S Yesmin ◽  
A Hashem ◽  
MS Islam
Keyword(s):  
Morphological Variation ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

Nodal segments from naturally grown Eclipta alba (L.) Hassk.were used as explants for organogenesis. Multiple shoots were obtained from the explants cultured on MS medium supplemented with various concentrations of BAP and Kn alone or in combination with NAA and IAA. Maximum number of multiple shoots (18.40±0.67) were induced on MS medium supplemented with 1.0 mg/l BAP and 0.1mg/NAA. In vitro raised shoots were cultured onto half and full strength MS medium supplemented with different concentration of IBA, IAA and NAA. The best root induction medium was found to be half strength MS containing 0.1 mg/l IBA where 96% shoots rooted. Regenerated plantlets grew normally without showing any morphological variation and flowered after 45 days of transplantation.Jahangirnagar University J. Biol. Sci. 4(1): 61-69, 2015 (June)

scholarly journals Micropropagation of Strawberry (Fragaria ananassa) through runner culture

2013 ◽  
Vol 38 (3) ◽  
pp. 467-472 ◽  
Author(s):  
M Ashrafuzzaman ◽  
SM Faisal ◽  
D Yadav ◽  
D Khanam ◽  
F Raihan
Keyword(s):  
In Vitro Propagation ◽  
Average Length ◽  
Root Induction ◽  
Fragaria Ananassa ◽  
Shoot Induction ◽  
Half Strength ◽  
Number Of Leaves ◽  
Number Of Shoots

In vitro propagation of strawberry was conducted at the Biotechnology Lab. of BARI, Joydebpur, Gazipur. For shoot induction, five BAP concentrations viz., 0.0 (Control), 0.5, 1.0, 1.5, and 2.0 mg/l and for root induction four IBA concentrations viz., 0.0 (Control), 0.5, 1.0, and 1.5 mg/l were used. The highest average number of shoots (7) and the highest average length (3.34 cm) of shoot was observed at the concentration of 0.5 mg/l BAP. The highest average number of leaves (5) was also observed at the same concentration. Among the five rooting concentrations, IBA @ 0.5 mg/l showed the best performance in all the parameters studied. The highest number (6) of roots/culture and the longest (3.05 cm) roots were also obtained from this concentration. Half strength MS media without IBA concentration did not show any response regarding root induction. DOI: http://dx.doi.org/10.3329/bjar.v38i3.16973 Bangladesh J. Agril. Res. 38(3): 467-472, September 2013

scholarly journals Micropropagation of the Endangered and Decorative Specie Dianthus serotinus Waldst. et Kit.

2013 ◽  
Vol 41 (2) ◽  
pp. 370 ◽  
Author(s):  
Marija MARKOVIĆ ◽  
Mihailo GRBIĆ ◽  
Matilda DJUKIĆ
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Shoot Multiplication ◽  
Naphthaleneacetic Acid ◽  
Endangered Plant ◽  
Ms Medium ◽  
Medium Ph ◽  
Half Strength ◽  
Terminal Buds

During past decades, great attention has been paid to propagation of endangered plant taxa in order to preserve biodiversity. The aim of this study was to optimize a protocol for in vitro propagation of the critically endangered and decorative species Dianthus serotinus Waldst. et Kit. The effects of different concentration of MS salt (Murashige and Skoog) of the culture, medium pH and different carbohydrates (sucrose, glucose, and fructose) on shoot multiplication were examined. The best results were obtained on half-strength MS (Murashige and Skoog) medium, whose pH was 5.8, with sucrose supplied at a concentration of 3%, when shoots with 1-2 nodes or shoot tips (with terminal buds only) were used as explants. The shoots were rooted (76.7%) on half-strength MS medium containing 0.5 mg∙L-1 NAA (1-naphthaleneacetic acid). The obtained plantlets were successfully acclimatized (89%) in a 4:1 mixture of peat and sand and they flowered the following year. Presented protocol enables successful in vitro propagation of D. serotinus.

Notes on In-vitro Propagation of Tecomella undulata (Sm.) Seem

2017 ◽  
Vol 24 (4) ◽  
pp. 217-220
Author(s):  
Rakesh Poria ◽  
K. Bangarwa ◽  
Kavita Sharma ◽  
R. Yadav
Keyword(s):  
In Vitro Propagation ◽  
Dark Period ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Root Induction ◽  
Step Procedure ◽  
Half Strength ◽  
Tecomella Undulata

Different explants of Tecomella undulata (Sm.) Seem. cultured on modified MS media gave significant differences for shoot regeneration and root differentiation. Plantlets were successfully developed using axillary bud and cotyledon as explants. There was no shoot formation from leaf explants. Maximum multiple shoots from axillary buds and cotyledons were obtained on medium containing MS major salts with BAP 4.0 mg/l + IAA 0.1 mg/l and BAP 5.0 mg/l + NAA 0.5 mg/l, respectively. Healthy roots developed from two step procedure. Isolated shoots were rooted by culturing on half strength MS liquid medium having IBA 2.5 mg/l for 48 hrs and then transferring to hormone free half strength solid MS medium. An initial dark period for 5-7 days favoured root induction. About 55 percent of in-vitro produced shoots were rooted.

scholarly journals Improved Rooting and Acclimatization of Micropropagated Hazelnut Shoots

HortScience ◽  
2004 ◽  
Vol 39 (7) ◽  
pp. 1688-1690 ◽  
Author(s):  
Mehmet Nuri Nas ◽  
Paul E. Read
Keyword(s):  
Survival Rate ◽  
Plant Growth ◽  
Butyric Acid ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Root Induction ◽  
Ex Vitro ◽  
Potential Use ◽  
Commercial Micropropagation

Microshoots of four hazelnut genotypes grown in vitro on Nas and Read medium (NRM) containing various combinations of CuSO4 • 5H2O and myo-inositol were successfully rooted and acclimatized ex vitro without any need of in vitro hardening treatments. Dipping of shoot bases in 1000 ppm indole-3-butyric acid (IBA) solution for 5 or 10 seconds followed by placement of shoots in plant growth regulator free NRM gave rise to formation of roots as early as 8 days. Shoots treated for 5 and 10 seconds rooted similarly, and depending on genotype, 88% to 98% rooting was observed within 15 days after treatment with IBA. Ex vitro survival of shoots three months after in vitro-root induction was 73% when shoots were treated with IBA for 5 seconds and 66% when shoots were treated for 10 seconds. The highest ex vitro survival rate (97%) 3 months after root induction was observed when shoots were treated with IBA solution for 10 seconds, and then cultured directly in peat pellets. Shoots developed good roots, and grew up to 70 cm in height 3 months after root induction. The potential use of rooting and acclimatization protocol for commercial micropropagation of hazelnut is presented.

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