scholarly journals (174) In Vitro Regeneration of Cornus kousa

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1052B-1052
Author(s):  
Denita Hadziabdic ◽  
Robert N. Trigiano ◽  
Stephen Garton ◽  
Mark T. Windham
Keyword(s):  
Woody Plant ◽  
In Vitro Regeneration ◽  
Root Production ◽  
Naphthaleneacetic Acid ◽  
Apical Buds ◽  
Cornus Kousa ◽  
Woody Plant Medium ◽  
Rooting Media ◽  
Indole 3 Acetic Acid

Axillary and apical buds from five Cornus kousa cultivars (`Little Beauty', `Samaritan', `Heart Throb', `Rosabella', and `Christian Prince') were initially established on two basal media, woody plant medium (WPM) and woody plant medium/broad leaved tree medium (BW), amended with the following concentrations of 6–benzylaminopurine (BA): 0, 2, 4, and 8 μm. After explants were transferred at 4-week intervals for 28 weeks beginning in April, only microshoots of `Samaritan', `HeartThrob' and `Rosabella', were harvested from proliferating cultures and placed on rooting media. `Little Beauty' and `Christian Prince' did not perform well in multiplication phase of tissue culture and were excluded from further studies. Rooting media contained WPM or BW supplemented with either 1-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) at the following concentrations: 0, 0.5, 1.5, 4.5, and 13.5 μm. Six weeks following rooting experiment, preliminary data were collected and results showed that total of nine plants rooted on both WPM and BW media supplemented with IBA, 17 plants rooted on media supplemented with NAA, and 14 plants rooted on media supplemented with IAA. These results indicated that NAA and IAA appeared to be better for root production on C. kousa cultivar microshoots than IBA. Additionally, WPM supported more root production, when compared to BW, even though both media resulted in rooted microshoots. Proliferating masses were placed on fresh medium with 2μm BA and were used again for the rooting projects.

scholarly journals IN VITRO FLOWERING OF MINIATURE ROSE CULTIVARS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 694e-694
Author(s):  
Michael Kane ◽  
Nancy L. Philman ◽  
Francis J. Marousky
Keyword(s):  
Woody Plant ◽  
Cut Flowers ◽  
Mineral Salts ◽  
Lateral Buds ◽  
Woody Plant Medium ◽  
Causative Agents ◽  
Ascorbic Acids ◽  
Indole 3 Acetic Acid ◽  
Stem Segments

Premature deterioration and/or wilting of cut flowers such as roses (“bent neck”) has been attributed to vascular blockage within the cut stem. Vascular blockage has been attributed to both the proliferation of bacteria in the cut flower water and/or to products exuded by the stem. Separation of these causative agents is prevented by the inability to obtain intact microbe-free flowers. With the objective to produce microbe-free flowers, 36 miniature rose cultivars were screened for their capacity to flower in vitro. Stem segments containing single lateral buds were surface sterilized in 1.05% (v/v) sodium hypochlorite and rinsed three times in sterile distilled deionized water. Buds were established on medium consisting of Murashige and Skoog mineral salts, Woody Plant Medium organics, 3.0% (w/v) sucrose, 0.5 mg/liter benzyladenine, 0.1 mg/liter indole-3-acetic acid, and 50 mg/liter each citric and ascorbic acids. Medium was solidified with 1.5 g/liter gelrite and 4 g/liter TC® agar. Of the 36 cultivars screened, eight (22%) grew poorly in vitro. Of the 28 responsive cultivars, 14 (50%) produced flower buds in vitro However, only six cultivars produced open flowers in vitro.

An Efficient in vitro Regeneration System for Australian-Grown Chickpea (Cicer arietinum) Cultivars

1995 ◽  
Vol 43 (5) ◽  
pp. 491 ◽  
Author(s):  
AL Adkins ◽  
ID Godwin ◽  
SW Adkins
Keyword(s):  
Cicer Arietinum ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Immature Cotyledon ◽  
Efficient Regeneration ◽  
Immature Cotyledons ◽  
Dim Light ◽  
Indole 3 Acetic Acid

A comparison of methods for efficient in vitro regeneration of Australian-grown chickpea (Cicer arietinum L.) cultivars was undertaken. The most efficient regeneration system was one where immature cotyledon and embryonic axis explants, 14-21 days post-pollination, were cultivated on Murashige and Skoog's salts with Gamborg's vitamins, 1.0, 3.0 or 5.2 mg L-1 zeatin, 0 or 35 μg L-1 indole-3-acetic acid, 30 g L-1 sucrose and 8 g L-1 Phytagar. The first embryoid structures appeared after 2 weeks of culture at 25 ± 1°C in dim light (150 μmol m-2 s-1) and formed directly on the edges of the immature cotyledons or petiole stumps. Between 10 and 20 structures were produced on each cotyledon explant in two cultivars, however, the embryogenic structures which developed on cv. Narayen were more efficiently transformed into shoots than far cv. Amethyst. An efficient regeneration medium (2 mg L-1 naphthaleneacetic acid, 1/2 Murashige and Skoog's salts with Gamborg's vitamins, and 0.5 g L-1 activated charcoal) was used to develop a portion of the shoots into morphologically normal plants growing in a vermiculite and soil potting mix in a growth room. Less efficient in vitro regeneration was observed when hypocotyl and shoot sections, and shoot apices were induced to form callus and plants by organogenesis. These plants could not be established in a potting mix. The amount and type of callus produced varied between explant type and cultivar.

In vitro propagation of Epacris impressa (Epacridaceae) and the effects of clonal material

2000 ◽  
Vol 48 (2) ◽  
pp. 215 ◽  
Author(s):  
J. Anthony ◽  
C. B. McLean ◽  
A. C. Lawrie
Keyword(s):  
In Vitro Propagation ◽  
Root Formation ◽  
Woody Plant ◽  
Multiple Shooting ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Main Determinant ◽  
Woody Plant Medium ◽  
Half Strength

A system of micropropagation has been developed for Epacris impressa Labill. (pink heath) (Epacridaceae), the floral emblem of Victoria, Australia. Only explants from glasshouse-grown plants treated with 1.2 g L–1 mancozeb were established successfully in vitro. Shoot material was very sensitive to surface-sterilisation, with 0.5% NaOCl for 5 min being optimal. Multiple shooting was induced optimally on Woody Plant Medium (WPM, Lloyd and McCown 1980) with 12–25 µM of the cytokinin 2iP (6-(γ,γ-dimethylallylamino) purine). Inclusion of the auxin IBA (indole-3-butyric acid) induced callus and reduced shooting. Rooting in vitro was greatest (up to 40%) with half-strength WPM and 16 µM IBA. Clones from individual plants varied in multiple shooting response to 2iP (0–49 µM) and root induction response to auxins (IBA and NAA (α-naphthaleneacetic acid), 0–43 µM). These results suggest that explant materials are the main determinant of success in in vitro propagation and that they require individual optimisation of treatments to maximise shoot and root formation.

Microspore embryogenesis in anther culture of three species of Populus and regeneration of dihaploid plants of Populustrichocarpa

1993 ◽  
Vol 23 (9) ◽  
pp. 1821-1825 ◽  
Author(s):  
Snorri Baldursson ◽  
Peter Krogstrup ◽  
Jens Viktor Nørgaard ◽  
Sven Bode Andersen
Keyword(s):  
Chromosome Number ◽  
Growth Regulator ◽  
Woody Plant ◽  
Adventitious Shoots ◽  
Microspore Embryogenesis ◽  
Naphthaleneacetic Acid ◽  
Chromosome Counts ◽  
Low Frequencies ◽  
Woody Plant Medium

Microspore embryogenesis was induced from in vitro cultured anthers of Populusbalsamifera L., Populusmaximowiczii A. Henry, and Populustrichocarpa Torr. & Gray. Embryoids were formed at low frequencies on a modified Murashige and Skoog's medium, supplemented with 5 μM 6-benzylaminopurine, 5.1 mM L-glutamine, and 6% maltose. Growth regulator combinations (0–10 μM 6-benzylaminopurine and naphthaleneacetic acid) affected embryogenesis only slightly but formation of nonembryogenic callus from the anthers increased with increasing concentration of naphthaleneacetic acid. One donor clone of P. trichocarpa produced 54 embryoids from as many anthers during the two years of study. Adventitious shoots were obtained from 31 of these embryoids on woody plant medium with 2.5 μM 6-benzylaminopurine and 0.005 μM naphthaleneacetic acid. Adventitious shoots from 25 different embryoids were successfully rooted on woody plant medium containing 0.25 μM indole-3-butyric acid and transplanted to soil. Isozyme analysis confirmed microspore origin of all plants studied, and chromosome counts revealed that most of them had doubled their chromosome number spontaneously.

scholarly journals (175) In Vitro Regeneration of Cladrastis kentukea

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1052C-1052
Author(s):  
Denita Hadziabdic ◽  
Robert N. Trigiano ◽  
Stephen Garton ◽  
Mark T. Windham ◽  
William E. Klingeman
Keyword(s):  
Growth Regulators ◽  
Root Length ◽  
Woody Plant ◽  
In Vitro Regeneration ◽  
Cambial Activity ◽  
Ms Medium ◽  
Total Root Length ◽  
Woody Plant Medium ◽  
Half Strength

Axillary buds from a single Cladrastis kentukea tree were initially cultured on two media, woody plant medium (WPM) and Murashige and Skoog (MS) containing 0, 1, 2, or 4 μm 6–benzylaminopurine (BA). Cultures were transferred to fresh media every 4 weeks. Elongated shoots were harvested after 39 weeks and transferred to half-strength MS medium supplemented with the following concentrations of IBA: 0, 3, 30, 100, and 300 μm for 3 d, then returned to half-strength MS without growth regulators. Explants exposed to 300 μm of IBA produced significantly more roots (75%) compared to explants exposed to other treatments. Fifty-four and 45% of the microshoots rooted when exposed to 100 and 30 μm IBA, respectively. Only 4% of the microshoots rooted when exposed to 3 μm IBA and none of the control microshoots rooted. Although the 300 μm treatment yielded the most rooted plantlets, there was significantly higher terminal meristem abortion compared to other treatments. There were no statistical differences between the numbers of roots and total root length among all treatments. Additionally, all microshoots that rooted had lenticels, suggesting that presence of lenticel cambial activity can possibly improve rooting abilities of selected microshoots. Rooted microshoots were gradually acclimatized to nonsterile environment.

scholarly journals Screening Pyrus Germplasm for in Vitro Rooting Response

HortScience ◽  
1995 ◽  
Vol 30 (6) ◽  
pp. 1292-1294 ◽  
Author(s):  
Barbara M. Reed
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Additional Treatment ◽  
In Vitro Rooting ◽  
Root Production ◽  
Naphthaleneacetic Acid ◽  
Indole 3 Acetic Acid ◽  
Rooting Response ◽  
Selection Of

Micropropagated shoots of 49 Pyrus species and cultivars and one selection of Pyronia veitchii (Trabut) Guillaumin were evaluated to test their responses to several in vitro rooting techniques. Auxin treatment was required for rooting in most cases. Eighteen of 50 accessions rooted ≥50% with a 15-second, 10-mm IBA dip followed by growth on medium with no growth regulators (NGR). Twelve accessions rooted on a medium with 10 μm IBA applied for 1 week followed by NGR medium for 3 weeks; NGR medium alone was effective for only two accessions. Twenty-eight accessions rooted poorly with IBA treatments; an additional treatment of a 15-second dip in 10 mm NAA followed by NGR medium produced ≥50% rooting for eight genotypes. Root production increased for 10 of 19 especially recalcitrant genotypes by 10 μm IAA treatments in darkness or at 30C and NAA dip treatments. Of rooted shoots, 73% survived acclimation in the greenhouse. Selections of Pyrus betulifolia Bunge, P. calleryana Decne., P. hondoensis Kikuchi and Nakai, P. koehnei C. Schneider, P. pashia Buch.-Ham. ex D. Don, P. pyrifolia (Burm.f.) Nakai cv. Shinseiki, P. regelii Rheder, P. ussuriensis Maxim., and the Pyronia veitchii selection failed to root in any of the treatments. Twenty-five of 32 P. communis L. cultivars and three other species rooted on at least one of the treatments. Chemical names used: 1-naphthaleneacetic acid (NAA), 1H-indole-3-butyric acid (IBA), 1H-indole-3-acetic acid (IAA).

scholarly journals In vitro regeneration of Didymopanax morototoni

2006 ◽  
Vol 66 (2a) ◽  
pp. 455-462 ◽  
Author(s):  
E. T. H. Franco ◽  
L. B. Gavioli ◽  
A. G. Ferreira
Keyword(s):  
Plant Regeneration ◽  
Somatic Embryos ◽  
Woody Plant ◽  
In Vitro Regeneration ◽  
Casein Hydrolysate ◽  
Forest Species ◽  
Embryogenic Calli ◽  
Woody Plant Medium ◽  
Germinated Seeds

The present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first step, woody plant medium (WPM) with casein hydrolysate (250 mgL-1 ) and 2,4-D (1.0 and 5.0 mgL-1) were used combined with kinetin (0.1 and 1.0 mgL-1). Twenty days after inoculation, the material was evaluated. Embryogenic calli were split, transferred to expression medium with several combinations of NAA and KIN, and moved to fresh medium after 60 days. Light did not interfere in embryo expression. Somatic embryos were formed either from individual cells or cell clusters. Plantlets were obtained in WPM medium and 10 gL-1 of sucrose with no plant regulator, or using 0.1 mgL-1 BAP and 0.5 mgL-1GA. Plantlets from somatic embryos of D. morototoni developed in 33% of the cases.

scholarly journals RESPON PERTUMBUHAN TUNAS SANINTEN (Castanopsis argentea (Blume) A.DC.) TERHADAP PEMBERIAN ZAT PENGATUR TUMBUH BAP DAN IAA SECARA IN VITRO The growth response of Saninten (Castanopsis argentea (Blume) A.DC.) In Vitro by Adding Plant Growth Regulator BAP

2018 ◽  
Vol 8 (3) ◽  
pp. 208-214
Author(s):  
Arum Sekar Wulandari ◽  
Erina Sulistiani ◽  
Esthi Liani Agustiani
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Growth Response ◽  
Woody Plant ◽  
Shoot Height ◽  
Woody Plant Medium ◽  
Indole 3 Acetic Acid

Saninten (Castanopsis argentea (Blume) A.DC.) is one of the member Fagaceae family which can produce wood and non-wood product. Micropropagation of saninten by in vitro has never been reported. This research aims to identify the growth response of saninten shoot by adding Plant Growth Regulator (PGR), they are 6-benzylaminopurine (BAP) and indole-3-acetic-acid (IAA) with different concentrations in propagation in vitro on woody plant medium (WPM). The research conducted a completely randomized design (CRD) factorial. They are PGR type and PGR concentration. The PGR type consist of two levels namely BAP and IAA. PGR concentration consist of three levels namely 0 mg/L BAP and IAA, 0.5 mg/L BAP or 0.1 mg/L IAA, and 1.0 mg/L BAP or 0.1 mg/L IAA. Parameters observed is the amount of shoot, shoot height, and callus growth. The combination of BAP (0, 0.5, 1 mg/L) and IAA (0, 0.1, 0.1 mg/L) haven’t produce optimal growth of shoot. WPM medium with 0.5 mg/L BAP was able to produce the best of percentage of shoot, the number of shoots, and shoot height growth. WPM medium with IAA concentration of 0.1 and 0.2 mg/L produce explant with callus.Key words: 6-benzylaminopurine (BAP), Castanopsis argentea, indole-3-acetic-acid (IAA), woody plant medium (WPM)

scholarly journals Micropropagation of Antelope Bitterbrush [Purshia tridentata (Pursh) DC.]

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 312-314 ◽  
Author(s):  
John L. Edson ◽  
David L. Wenny ◽  
Annette Leege-Brusven
Keyword(s):  
Woody Plant ◽  
Naphthaleneacetic Acid ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Axillary Shoots ◽  
Full Strength ◽  
Woody Plant Medium ◽  
Half Strength ◽  
In Vitro Survival

In vitro—derived microshoots of antelope bitterbrush, incubated for 1 month in media supplemented with 0.44 μm BA, grew 0.8 and 1.1 cm longer in woody plant medium (WPM) compared to full-strength and half-strength Murashige and Skoog (MS) media, respectively. Explants cultured in WPM supplemented with 0.44 μm BA and 0.54 μm NAA produced a mean of five axillary shoots per explant. Explants dipped in 0.1% IBA or 0.1% NAA rooted best in 0.1% IBA with 89% success ex vitro vs. 60% success in vitro. Survival of acclimatized plantlets rooted ex vitro was 95%, while 50% survived when rooted in vitro. After 1 year of greenhouse growth, 98% of plantlets survived and flowered. Chemical names used: benzyladenine (BA), 3-indolebutyric acid (IBA), 1-naphthaleneacetic acid (NAA).

scholarly journals In Vitro and Macropropagation of the Wildflower Dyssodia pentacheta (D. C.) Robins

HortScience ◽  
1991 ◽  
Vol 26 (12) ◽  
pp. 1555-1557
Author(s):  
Thomas W. Zimmerman ◽  
Fred T. Davies ◽  
Jayne M. Zajicek
Keyword(s):  
Shoot Growth ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Nodal Explant ◽  
System A ◽  
Woody Plant Medium

Dyssodia pentacheta, a prostrate-growing perennial Texas wildflower with potential for use in low-maintenance landscapes, was propagated in vitro and by stem cuttings under mist. Optimum rooting for IBA-treated semihardwood terminal stem cuttings (3 to 30 mm IBA) and in vitro-grown nodal segments (30 to 100 mm IBA) occurred after 4 weeks under an intermittent mist system. A 300-mm IBA basal dip was lethal to macroand microcuttings. In vitro, D. pentacheta produced more shoots per nodal explant on Woody Plant Medium (2 g Gelrite/liter) with 1 to 10 μ m BA than with combinations of BA and 0.5 μm NAA. After shoot proliferation, the shoots were subculture twice and grown on growth regulator-free medium. When maintaining D. pentacheta in vitro on media devoid of plant growth regulators, 1% sucrose was more effective than 2% for promoting shoot growth and suppressing apparent production of phenolics. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA); 1H-indole-3-butyric acid (IBA); 1-naphthaleneacetic acid (NAA).

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