Determination of Inorganic Selenium Species in Marine Waters by Hydride Generation - AFS

2004 ◽  
Vol 57 (10) ◽  
pp. 937 ◽  
Author(s):  
Bronwyn D. Wake ◽  
Edward C. V. Butler ◽  
Alison M. Featherstone ◽  
Patti Virtue ◽  
Bernard Averty ◽  
...  
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Hydride Generation ◽  
Reduction Step ◽  
Marine Waters ◽  
Atomic Fluorescence ◽  
Inorganic Selenium ◽  
Cryogenic Trapping ◽  
Better Than

A method based on hydride generation with cryogenic trapping and atomic fluorescence detection was developed that is capable of determining both inorganic Se species (SeIV and SeVI) while at sea. Evaluation of the system for optimal performance was made for each stage of the analysis and detection sequence, as well as for the SeVI reduction step. A detection limit of 0.4 ng L−1 Se in a 10 mL sample was achieved using this method. Precision was better than 3.5% for 25, 100, and 1000 ng L−1 SeIV standard solutions. Accuracy was determined by recovery studies using natural samples and a certified reference seawater (NASS-5).

scholarly journals Methods development for the determination of methyl mercury in sediment samples using gas chromatography with atomic fluorescence detection

2013 ◽  
Vol 16 (2) ◽  
pp. 53-60
Author(s):  
An Quoc Trieu ◽  
Huy Phuong Tran ◽  
Dong Van Nguyen
Keyword(s):  
Gas Chromatography ◽  
Detection Limit ◽  
Fluorescence Detection ◽  
Potassium Oxalate ◽  
Fluorescence Detector ◽  
Atomic Fluorescence ◽  
Sediment Samples ◽  
Methods Development ◽  
Instrumental System

An analytical method for methylmercury (MeHg) using gas chromatography with atomic fluorescence detection is studied. The instrumental system is made based on a old gas chromatograph interfaced with an atomic fluorescence detector which is specific to Hg, currently available in our lab. Operating parameters for the GC-AFS system are optimised and analytical performances of the system are verified by quality control chart for stability. MeHg in sediment is leached and extracted to dichloromethane (DCM) in the presence of nitric acid, potassium chloride and copper sulfate. DCM in the extract is purged and MeHg is back extracted to aqueous phase followed by ethylation with sodium tetraethylborate in acetate buffer pH 5.3 containing potassium oxalate. The ethylated MeHg is then extracted to hexane and injected to GC-AFS for quantitation. The instrumental detection limit and method detection limit are 0.5 pg MeHg and 0.029 ppb MeHg (as Hg), respectively. The method can be applied for the determination of MeHg in soil, sludge and sediment samples.

Determination of antimony by continuous hydride generation coupled with non-dispersive atomic fluorescence detection

1995 ◽  
Vol 10 (11) ◽  
pp. 969 ◽  
Author(s):  
Alessandro D'ulivo ◽  
Leonardo Lampugnani ◽  
Giovanna Pellegrini ◽  
Roberto Zamboni
Keyword(s):  
Fluorescence Detection ◽  
Hydride Generation ◽  
Atomic Fluorescence

Alcohol Determination by HPLC with Postcolumn Derivatization Based on the Thiosulfate-catalyzed Reaction of Alcohols and Cerium(IV) and Fluorescence Detection of Cerium(III)

2020 ◽  
Vol 16 ◽  
Author(s):  
Ikko Mikami ◽  
Eri Shibayama ◽  
Kengo Takagi
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Reaction Rate ◽  
Detection Method ◽  
Fluorescence Detector ◽  
Reaction Solution ◽  
Optimum Detection ◽  
Postcolumn Derivatization ◽  
Sensitive Method

Background: Determination of a reducing substance based on the reaction between Ce(IV) and a reducing substance and fluorescence detection of Ce(III) generated has been reported as a selective and sensitive method. However, this method could not be applied to the determination of alcohol due to the low reaction rate of alcohol and Ce(IV). Objective: We found that thiosulfate catalytically enhanced reaction of alcohols (such as, methanol, ethanol, and propanol) and Ce(IV). Utilizing this effect, we developed a new method for the determination of alcohols. Results: In the presence of thiosulfate, an increase in fluorescence intensity was detected by injecting alcohol at concentrations of several millimolar, whereas it was not observed even at the concentration of 10% v/v (2 M for ethanol) in the absence of thiosulfate. The optimum detection conditions were determined to be 4.0 mM Ce(IV) sulfate and 0.50 mM thiosulfate, and the detection limit (S/N = 3) of ethanol under these conditions was 1 mM. In the calibration curves, changes in the slope were observed when the alcohol concentrations were approximately 10–25 mM. Using a thiosulfate solution containing ethanol as the reaction solution, a calibration curve without any change in slope was obtained, although the concentration of ethanol at the detection limit increased. The alcohols in the liquor and fuel were successfully analyzed using the proposed detection method as a postcolumn reaction. Conclusion: This new alcohol detection method using a versatile fluorescence detector can be applied to the postcolumn reaction of HPLC omitting need of time-consuming pretreatment processes.

Determination of Selenium in Eggs of Different Birds Using Sample Digestion in a Reflux System and Hydride Generation Atomic Fluorescence Spectrometry

2016 ◽  
Vol 12 (2) ◽  
pp. 102-107 ◽  
Author(s):  
Walter N.L. dos Santos ◽  
Dannuza D. Cavalcante ◽  
Paula D.N. Gomes ◽  
Daniele C.M.B. Santos ◽  
Luciana B. Oliveira ◽  
...  
Keyword(s):  
Hydride Generation ◽  
Fluorescence Spectrometry ◽  
Atomic Fluorescence Spectrometry ◽  
Atomic Fluorescence ◽  
Sample Digestion
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