Selective Transport of Water-Soluble Proteins from Aqueous to Ionic Liquid Phase via a Temperature-Sensitive Phase Change of These Mixtures

Mixtures of some ionic liquids (ILs) and water show reversible phase change between a homogeneous mixture and phase-separated state by a small change in temperature. Some water-soluble proteins have been migrated from the aqueous to the IL phase. When tetrabutylphosphonium 2,4,6-trimethylbenzenesulfonate was used as an IL, cytochrome c (Cyt.c) was found to be extracted from the water phase to the IL phase. Conversely, both horseradish peroxidase (HRP) and azurin remained in the aqueous phase. This selective extraction was comprehended to be due to the difference in solubility of these proteins in both phases. The separated aqueous phase contained a small amount of IL, which induced the salting-out of Cyt.c. On the other hand, condensed IL phase promoted the salting-in of Cyt.c. As a result, Cyt.c was preferably dissolved in the hydrated IL phase rather than aqueous phase. In the case of HRP, there was only a salting-out profile upon increasing the concentration of IL, which induced selective dissolution of HRP in the aqueous phase. These results clearly suggest that the profile of salting-out and salting-in for proteins is the key factor to facilitate the selective extraction of proteins from aqueous to the IL phase.

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Trypsin inhibitor from starchy endosperm of rye seeds

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1974.003 ◽

2015 ◽

Vol 43 (1) ◽

pp. 27-37

Author(s):

A. Pawłowski

Keyword(s):

Molecular Weight ◽

Ammonium Sulphate ◽

Trypsin Inhibitor ◽

Soluble Proteins ◽

Water Soluble ◽

Starchy Endosperm ◽

Salting Out ◽

Isolation And Purification

A method for the preparation of a trypsin inhibitor from starchy endosperm of rye seeds is described. Isolation and purification comprised the following steps: salting out of the water-soluble proteins at pH 4.5 with ammonium sulphate (0.4 saturation), followed by chromatography on CM-Cellulose, Sephadex G-100 and SE Sephadex C-25. The finally purified preparation of inhibitor was found to be homogeneous by both chromatographic and electrophoretic analyses. The preparation inhibited trypsin and chymotrypsin but was inactive against papain, kallikrein and pepsin. The molecular weight of the inhibitor was calculated to foe about 10 000.

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A method for selective separation of scandium (III) from yttrium and a number of rare-earth elements for its subsequent determination

Industrial laboratory Diagnostics of materials ◽

10.26896/1028-6861-2018-84-11-23-27 ◽

2018 ◽

Vol 84 (11) ◽

pp. 23-27

Author(s):

M. I. Degtev ◽

A. A. Yuminova ◽

A. S. Maksimov ◽

A. P. Medvedev

Keyword(s):

Rare Earth ◽

Aqueous Phase ◽

Selective Isolation ◽

Optimum Ratio ◽

Extraction Condition ◽

Sulfosalicylic Acid ◽

Salting Out ◽

Subsequent Determination ◽

Toxic Organic Solvents ◽

Metal Ions Extraction

The possibility of using an aqueous stratified system of antipyrine — sulfosalicylic acid — water for the selective isolation of scandium macro- and microquantities for subsequent determination is studied. The proposed extraction system eliminates the usage of toxic organic solvents. The organic phase with a volume of 1.2 to 2.0 ml, resulting from delamination of the aqueous phase containing antipyrine and sulfosalicylic acid is analysed to assess the possibility of using such systems for metal ions extraction. Condition necessary for the formation of such a phase were specified: the ratio of the initial components, their concentration, presence of inorganic salting out agents. The optimum ratio of antipyrine to sulfosalicylic acid is 2:1 at concentrations of 0.6 and 0.3 mol/liter in a volume of the aqueous phase of 10 ml. The obtained phase which consists of antipyrinium sulfosalicylate, free antipyrine and water, quantitatively extracts macro- and microquantities of scandium at pH = 1.54. Macro- and microquantities of yttrium, terbium, lanthanum, ytterbium and gadolinium are not extracted under the aforementioned conditions thus providing selective isolation of scandium from the bases containing yttrium, ytterbium, terbium, lanthanum, and gadolinium.

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ANALISA KANDUNGAN BETA-GLUKAN LARUT AIR DAN LARUT ALKALI DARI TUBUH BUAH JAMUR TIRAM (Pleurotus ostreatus) DAN SHIITAKE (Lentinus edodes)

Jurnal Sains dan Teknologi Indonesia ◽

10.29122/jsti.v13i3.894 ◽

2013 ◽

Vol 13 (3) ◽

Author(s):

Netty Widyastuti ◽

Teguh Baruji ◽

Henky Isnawan ◽

Priyo Wahyudi ◽

Donowati Donowati

Keyword(s):

Molecular Structure ◽

Molecular Weight ◽

Immune System ◽

Pleurotus Ostreatus ◽

Water Soluble ◽

Low Molecular Weight ◽

Lentinus Edodes ◽

Beta Glucan ◽

Oyster Mushrooms ◽

The Difference

Beta glucan is a polysaccharide compound, generally not soluble inwater and resistant to acid. Beta glucan is used as an immunomodulator (enhancing the immune system) in mammals is usually a beta-glucan soluble in water, easily absorbed and has a low molecular weight. Several example of beta-glucan such as cellulose (β-1 ,4-glucan), lentinan (β-1 0.6-glucan) and (β-1 ,3-glucan), pleuran (β-1, 6 and β-1 ,3-glucan) are isolated from species of fungi Basidiomycota include mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes).The purpose of thisresearch activity is to obtain beta-glucan compound that can be dissolved in water and in alkali derived from fungi Basidiomycota, i.e, Oyster mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes). The result of beta-glucan compared to characterize the resulting beta glucan that is molecular structure . The difference of beta glucan extraction is based on the differences in solubility of beta-glucan. Beta glucan could be water soluble and insoluble water.

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Extraction of hafnium(IV) with organophosphorus reagents from solutions of mineral acids mixtures

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19793656 ◽

1979 ◽

Vol 44 (12) ◽

pp. 3656-3664

Author(s):

Oldřich Navrátil ◽

Jiří Smola ◽

Rostislav Kolouch

Keyword(s):

Ionic Strength ◽

Aqueous Phase ◽

Organic Phase ◽

Perchloric Acid ◽

Synergic Effect ◽

Salting Out ◽

Initial Concentration ◽

Mixed Complexes ◽

Synergic Extraction ◽

Mineral Acids

Extraction of hafnium(IV) was studied from solutions of mixtures of perchloric and nitric acids and of perchloric and hydrochloric acids for constant ionic strength, I = 2, 4, 6, or 8, and for cHf 4 . 10-4 mol l-1. The organic phase was constituted by solutions of some acidic or neutral organophosphorus reagents or of 2-thenoyltrifluoroacetone, 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone, or N-benzoyl-N-phenylhydroxylamine in benzene, chloroform, or n-octane. A pronounced synergic extraction of hafnium proceeds only on applying organophosphorus reagents from an aqueous phase whose acidity is not lower than 3M-(HClO4 + HNO3) or 5M-(HClO4 + HCl). The synergic effect was not affected markedly by a variation of the initial concentration of hafnium in the range 1 . 10-8 -4 .10-4 mol l-1, it lowered with increasing initial concentration of the organophosphorus reagent and decreasing concentration of the H+ ions. It is suggested that the hafnium passes into the organic phase in the form of mixed complexes, the salting-out effect of perchloric acid playing an appreciable part.

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Analysis of water-soluble proteins and peptides from Bt-transgenic and non-transgenic maize varieties by capillary electrophoresis

Collection Symposium Series ◽

10.1135/css200911110 ◽

2009 ◽

Author(s):

Petra Sázelová ◽

Václav Kašička ◽

Elena Ibañez ◽

Alejandro Cifuentes

Keyword(s):

Capillary Electrophoresis ◽

Soluble Proteins ◽

Transgenic Maize ◽

Water Soluble ◽

Maize Varieties ◽

Proteins And Peptides

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A Model of Wheat Grain Growth and Its Applications to Different Temperature and Carbon Dioxide Levels

Functional Plant Biology ◽

10.1071/pp9950843 ◽

1995 ◽

Vol 22 (5) ◽

pp. 843 ◽

Cited By ~ 8

Author(s):

YP Wang ◽

RM Gifford

Keyword(s):

Growth Rate ◽

Grain Growth ◽

Temperature Response ◽

Rate Sensitivity ◽

Temperature Sensitive ◽

Potential Growth ◽

Carbon Supply ◽

The Difference ◽

Carbon Dioxide Levels ◽

Kernel Growth

Kernel growth after anthesis is simulated as a function of the potential kernel growth rate, current photosynthate production and mobilisation of stored reserves. The potential growth rate of the kernel is simulated as two temperature-sensitive processes, cell production and cell growth. The difference between the potential and actual growth rates of the kernel depends on the carbon supply to the free space of the kernel endosperm, while the carbon supply is itself affected by the actual kernel growth rate. Sensitivity analysis showed that the growth rate of the grain per plant is most sensitive to the potential growth rate of the kernel and number of kernels per plant. This model is able to simulate the observed rates of grain growth and leaf senescence from anthesis to physiological maturity for wheat plants grown in two CO2 concentrations. The simulated temperature response of grain growth agrees well with the experimenal observations.

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