Analysis of water-soluble proteins and peptides from Bt-transgenic and non-transgenic maize varieties by capillary electrophoresis

2009 ◽  
Author(s):  
Petra Sázelová ◽  
Václav Kašička ◽  
Elena Ibañez ◽  
Alejandro Cifuentes
Keyword(s):  
Capillary Electrophoresis ◽  
Soluble Proteins ◽  
Transgenic Maize ◽  
Water Soluble ◽  
Maize Varieties ◽  
Proteins And Peptides

Applications of capillary electrophoresis for rapidly separating and characterizing water-soluble proteins of wheat grains

2013 ◽  
Vol 41 (4) ◽  
pp. 601-612
Author(s):  
C. Han ◽  
Z. Yu ◽  
S. Feng ◽  
D. Lv ◽  
X. Yan ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Soluble Proteins ◽  
Water Soluble ◽  
Wheat Grains

scholarly journals Extraction and separation of water-soluble proteins from Bacillus thuringiensis -transgenic and non-transgenic maize species by CZE

2009 ◽  
Vol 32 (21) ◽  
pp. 3801-3808 ◽  
Author(s):  
Petra Sázelová ◽  
Václav Kašička ◽  
Elena Ibáñez ◽  
Alejandro Cifuentes
Keyword(s):  
Bacillus Thuringiensis ◽  
Soluble Proteins ◽  
Transgenic Maize ◽  
Water Soluble ◽  
Extraction And Separation

scholarly journals Rapid separation and characterization of grain water-soluble proteins in bread wheat cultivars (Triticum aestivum L.) by capillary electrophoresis

2008 ◽  
Vol 88 (4) ◽  
pp. 843-848 ◽  
Author(s):  
Ai-li Wang ◽  
Yu-he Pei ◽  
Xiao-hui Li ◽  
Yan-zhen Zhang ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Capillary Electrophoresis ◽  
Bread Wheat ◽  
Biochemical Markers ◽  
Soluble Proteins ◽  
Water Soluble ◽  
Wheat Cultivars ◽  
Organic Modifiers ◽  
Glycine Buffer

Water-soluble (WS) proteins in wheat grain are considered to represent the suite of biologically active enzymes and enzyme inhibitors in the grain. In this study, a rapid capillary electrophoresis (CE) method for WS protein separations was developed using untreated fused-silica columns and an acidic phosphate-glycine buffer system. In order to optimize the resolution and reproducibility of CE separation, different protein extraction methods, organic modifiers in phosphate-glycine buffer and capillary electrophoresis conditions, including capillary length and inner diameter (ID), operating temperature, performance voltages, sample injection times, etc., were investigated. High resolution and reproducibility of WS proteins were achieved using 20% ethanol as the extracting buffer. The optimal condition to separate these proteins was 50 μm ID × 31.5 cm (26.5 cm to the detector) capillary at 11.0 kV and 35°C. The optimum buffer was 0.1 M phosphate-glycine (pH 2.5) containing 20% acetonitrile (ACN) and 0.05% hydroxylpropylmethylcellulose. Using this method, the WS proteins were well separated in less than 10 min. A total of 120 Chinese bread wheat cultivars were analyzed. The CE patterns of most bread wheat cultivars showed a higher level of polymorphisms compared with SDS-PAGE patterns. All cultivars analyzed could be readily differentiated based on their WS protein profiles. Results indicate that the WS proteins are useful biochemical markers for wheat genetics and breeding research and CE is expected to become a new and powerful tool for the separation and characterization of grain WS proteins in bread wheat. Key words: Triticum aestivum, bread wheat, water-soluble proteins, capillary electrophoresis, biochemical markers

Capillary electrophoretic profiling of tryptic digests of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species

2012 ◽  
Vol 134 (3) ◽  
pp. 1607-1615 ◽  
Author(s):  
Petra Sázelová ◽  
Václav Kašička ◽  
Carlos Leon ◽  
Elena Ibáñez ◽  
Alejandro Cifuentes
Keyword(s):  
Bacillus Thuringiensis ◽  
Soluble Proteins ◽  
Transgenic Maize ◽  
Water Soluble ◽  
Capillary Electrophoretic

scholarly journals Chiral Selectors in Capillary Electrophoresis: Trends During 2017–2018

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1135 ◽  
Author(s):  
Raymond B. Yu ◽  
Joselito P. Quirino
Keyword(s):  
Capillary Electrophoresis ◽  
Molecular Mass ◽  
Chiral Separation ◽  
Chiral Separations ◽  
Water Soluble ◽  
Resonance Spectroscopy ◽  
Electrokinetic Chromatography ◽  
Chiral Selectors ◽  
Analytical Technique ◽  
Pharmaceutical Industries

Chiral separation is an important process in the chemical and pharmaceutical industries. From the analytical chemistry perspective, chiral separation is required for assessing the fit-for-purpose and the safety of chemical products. Capillary electrophoresis, in the electrokinetic chromatography mode is an established analytical technique for chiral separations. A water-soluble chiral selector is typically used. This review therefore examines the use of various chiral selectors in electrokinetic chromatography during 2017–2018. The chiral selectors were both low and high (macromolecules) molecular mass molecules as well as molecular aggregates (supramolecules). There were 58 papers found by search in Scopus, indicating continuous and active activity in this research area. The macromolecules were sugar-, amino acid-, and nucleic acid-based polymers. The supramolecules were bile salt micelles. The low molecular mass selectors were mainly ionic liquids and complexes with a central ion. A majority of the papers were on the use or preparation of sugar-based macromolecules, e.g., native or derivatised cyclodextrins. Studies to explain chiral recognition of macromolecular and supramolecular chiral selectors were mainly done by molecular modelling and nuclear magnetic resonance spectroscopy. Demonstrations were predominantly on drug analysis for the separation of racemates.

Use of High Ionic Strength Buffers for the Separation of Proteins and Peptides with Capillary Electrophoresis

1992 ◽  
Vol 15 (6-7) ◽  
pp. 1143-1161 ◽  
Author(s):  
F. A. Chen ◽  
L. Kelly ◽  
R. Palmieri ◽  
R. Biehler ◽  
H. Schwartz
Keyword(s):  
Capillary Electrophoresis ◽  
Ionic Strength ◽  
High Ionic Strength ◽  
Separation Of Proteins ◽  
Proteins And Peptides
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