Gibberellic acid detection of dwarf offtypes in micropropagated Cavendish bananas

1996 ◽  
Vol 36 (2) ◽  
pp. 237 ◽  
Author(s):  
OP Damasco ◽  
ID Godwin ◽  
MK Smith ◽  
SW Adkins
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Culture ◽  
New Guinea ◽  
Plant Establishment ◽  
Musa Spp ◽  
Naturally Occurring ◽  
Identification Criterion ◽  
Dwarf Cultivar

Detection of dwarf offtypes produced by micropropagation of Cavendish bananas (Musa spp.) cultivars New Guinea Cavendish and Williams was achieved by spraying gibberellic acid (GA3) solution (289 pmol/L) onto deflasked plants and measuring various plantlet responses. The most useful identification criterion was elongation of the sheath of the first leaf to form after GA3 application. Elongation of this structure was about 2-fold greater in normal plants than observed in dwarfs. Similar measurements taken earlier during in vitro culture or later during plant establishment in soil were not as useful in discriminating between normals and dwarfs as the measurements made at deflasking. The similar GA3-induced elongation response of the dwarf offtype and that of the naturally occurring dwarf cultivar Dwarf Parfitt suggests that the mechanism for dwarfism could be the same in the 2 cases.

scholarly journals Embryo Rescue in Wide Crosses in Arachis. 3. In vitro Culture of Peg Tips of A. hypogaea Selfs and Interspecific Hybrids1

1987 ◽  
Vol 14 (2) ◽  
pp. 70-74 ◽  
Author(s):  
J. P. Moss ◽  
H. T. Stalker
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Culture ◽  
Successful Treatment ◽  
Interspecific Hybrids ◽  
Embryo Rescue ◽  
Wide Crosses ◽  
Reproductive Tissues ◽  
Globular Stage ◽  
Callus Production

Abstract Embryo rescue in wide crosses in Arachis has only been achieved from culturing ovules excised from well developed pods, or from immature pods derived from flowers treated with gibberellic acid (GA) and which had embryos large enough to dissect without injury. The objective of this study was to determine whether reproductive tissues could grow in vitro without the need to dissect them from the peg tip and to determine the effects of GA application on flowers at the time of pollination, age of peg when cultured, and the presence of the peg meristem on reproductive growth, callus production, and peg elongation in vitro. Pegs elongated in culture only when the peg meristem was not removed. Ovules enlarged and grew out of the surrounding peg tissue in 3.8 to 32.8% of the cultures. Significantly more ovules grew when the peg meristem was removed (p < 0.01) and when 10- and 20- day-old pegs were cultured (p < 0.05). Overall, the most successful treatment for growth of ovules was treating flowers with GA at pollination and culturing without the peg meristem 10 days after pollination when 25.0 and 32.8% of all hybrid and selfed ovules, respectively, grew. Embryo growth was observed in an average of 8.4 and 17.6% of embryo sacs in hybrid and self peg tips, respectively, with several embryos reaching the globular stage after 21 days in vitro. This illustrates the potential for culturing young reproductive tissues of Arachis to recover interspecific hybrids.

Isohexenylnaphthazarins from Lithospermum canescens (Michx.) Lehm. and Arnebia euchroma (Royle) Jonst. in vitro culture

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Graikou ◽  
H Damianakos ◽  
K Syklowska-Baranek ◽  
A Pietrosiuk ◽  
M Jeziorek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Arnebia Euchroma ◽  
Lithospermum Canescens

Effect of antioxidants on in vitro culture of pomegranate cv. Sindhuri

2018 ◽  
Vol 34 (2) ◽  
pp. 311-318
Author(s):  
Ravi Kumar ◽  
◽  
M.L. Jakhar ◽  
Komal Sekhawat ◽  
Swarnlata Kumawat ◽  
...  
Keyword(s):  
In Vitro Culture

Leaf Color of Plants Regenerated through In Vitro Culture from Variegated Leaf Segments of Caladium.

1993 ◽  
Vol 62 (3) ◽  
pp. 619-624 ◽  
Author(s):  
Yu Zhu ◽  
Tetsuyuki Takemoto ◽  
Susumu Yazawa
Keyword(s):  
In Vitro Culture ◽  
Leaf Color ◽  
Leaf Segments ◽  
Variegated Leaf

In vitro Culture of Human PBMCs

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (3) ◽  
Author(s):  
Santosh Panda ◽  
Balachandran Ravindran
Keyword(s):  
In Vitro Culture ◽  
Human Pbmcs

Morphogenesis of a Dissected Birch Leaf in vitro Culture

2020 ◽  
Vol 51 (6) ◽  
pp. 397-409
Author(s):  
O. S. Mashkina ◽  
T. M. Tabatskaya
Keyword(s):  
In Vitro Culture

scholarly journals Assessment of Photo-Induced Cytotoxic Activity of Cachrys sicula and Cachrys libanotis Enriched-Coumarin Extracts against Human Melanoma Cells

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 123
Author(s):  
Mariangela Marrelli ◽  
Maria Rosaria Perri ◽  
Valentina Amodeo ◽  
Francesca Giordano ◽  
Giancarlo A. Statti ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Melanoma Cells ◽  
Therapeutic Approaches ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Naturally Occurring ◽  
Quantitative Analyses ◽  
Solid Liquid ◽  
Uva Radiation

Photochemotherapy is one of the most interesting current therapeutic approaches for the treatment of melanoma. Different classes of naturally occurring phytochemicals demonstrated interesting photoactive properties. The aim of this study was to evaluate the photocytotoxic potential of two Cachrys species from Southern Italy: C. sicula and C. libanotis (Apiaceae). The enriched-coumarin extracts were obtained from aerial parts through both traditional maceration and pressurized cyclic solid-liquid (PCSL) extraction using Naviglio extractor®. Qualitative and quantitative analyses of furanocoumarins were performed with GC-MS. The photocytotoxic effects were verified on C32 melanoma cells irradiated at a dose of 1.08 J/cm2. The apoptotic responses were also assessed. Moreover, phenolic content and the in vitro antioxidant potential were estimated. Xanthotoxin, bergapten, and isopimpinellin were identified. All the samples induced concentration-dependent photocytotoxic effects (IC50 ranging from 3.16 to 18.18 μg/mL). The C. libanotis sample obtained with Naviglio extractor® was the most effective one (IC50 = 3.16 ± 0.21 μg/mL), followed by C. sicula sample obtained with the same technique (IC50 = 8.83 ± 0.20 μg/mL). Both Cachrys samples obtained through PCSL induced up-regulation of apoptotic signals such as BAX (Bcl2-associated X protein) and PARP (poly ADP-ribose polymerase) cleavage. Moreover, these samples proved to be more photoactive, giving a greater upregulation of p21 protein in the presence of UVA radiation. Obtained results suggest that investigated species could be promising candidates for further investigations aimed to find new potential drugs for the photochemotherapy of skin cancer.

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