A PRp27 gene of Nicotiana benthamiana contributes to resistance to Pseudomonas syringae pv. tabaci but not to Colletotrichum destructivum or Colletotrichum orbiculare

2009 ◽  
Vol 36 (4) ◽  
pp. 351 ◽  
Author(s):  
Weilong Xie ◽  
Paul H. Goodwin

NbPRp27 from Nicotiana benthamiana Domin. is highly similar to NtPRp27, which is a secreted protein from Nicotiana tabacum L. belonging to pathogen-inducible genes comprising the PR17 family of pathogenesis-related proteins. A collection of related genes from plants in several plant families showed that their deduced amino acid sequences clustered according to plant family. Expression of NbPRp27 was not detectable in healthy leaves or stems but was expressed at high levels in roots. Expression was induced by wounding, BTH, ethylene, methyl jasmonate, ABA and NAA, but not by drought, heat or cold stress. Expression was induced by infection with the hemibiotrophic pathogens, Colletotrichum destructivum, Colletotrichum orbiculare and Pseudomonas syringae pv. tabaci. For infections with the Colletotrichum species, expression increased more slowly during biotrophy than necrotrophy, but the reverse was true for P. syringae pv. tabaci. Virus-induced silencing of NbPRp27 did not affect the lesion number produced by the Colletotrichum species but did reduce basal resistance to P. syringae pv. tabaci permitting higher bacterial populations. Based on sequence similarities, PRp27 proteins have been hypothesised to have protease activity and may contribute to resistance by exhibiting direct antimicrobial activity in the apoplast, releasing of antimicrobial compounds from the plant matrix or releasing elicitors from pathogens to induce resistance.

2008 ◽  
Vol 35 (11) ◽  
pp. 1112 ◽  
Author(s):  
C. P. Wijekoon ◽  
P. H. Goodwin ◽  
T. Hsiang

Epoxide hydrolase hydrates epoxides to vicinal diols in the phyto-oxylipin peroxygenase pathway resulting in the production of epoxy alcohols, dihydrodiols, triols and epoxides, including many lipid epoxides associated with resistance. Two epoxide hydrolase genes from Nicotiana benthamiana L., NbEH1.1 and NbEH1.2, were amplified from coding DNA of leaves during a susceptible response to the hemibiotrophic pathogens, Colletotrichum destructivum O’Gara, Colletotrichum orbiculare Berk. and Mont. von Arx. or Pseudomonas syringae pv. tabaci Wolf and Foster, or the hypersensitive resistance response to P. syringae pv. tabaci expressing avrPto. Increases in expression of NbEH1.1 generally occurred during the late biotrophic and necrotrophic stages in the susceptible responses and before the hypersensitive response. NbEH1.2 expression was not significantly induced by C. orbiculare but was induced by C. destructivum, P. syringae pv. tabaci and P. syringae pv. tabaci expressing avrPto, although to a lesser degree than NbEH1.1. Virus-induced gene silencing of NbEH1.1 delayed the appearance of lesions for C. destructivum, reduced populations of P. syringae pv. tabaci and increased populations of P. syringae pv. tabaci expressing avrPto. The importance of epoxide hydrolase during pathogen attack may be related to its roles in detoxification, signalling, or metabolism of antimicrobial compounds.


2009 ◽  
Vol 21 (8) ◽  
pp. 2517-2526 ◽  
Author(s):  
Shigeyuki Tanaka ◽  
Nobuaki Ishihama ◽  
Hirofumi Yoshioka ◽  
Aurélie Huser ◽  
Richard O'Connell ◽  
...  

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17306 ◽  
Author(s):  
Ourania I. Pavli ◽  
Georgia I. Kelaidi ◽  
Anastasia P. Tampakaki ◽  
George N. Skaracis

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