Improvement of salt and waterlogging tolerance in wheat: comparative physiology of Hordeum marinum-Triticum aestivum amphiploids with their H. marinum and wheat parents

Hordeum marinum Huds. is a waterlogging-tolerant halophyte that has been hybridised with bread wheat (Triticum aestivum L.) to produce an amphiploid containing both genomes. This study tested the hypothesis that traits associated with waterlogging and salinity tolerances would be expressed in H. marinum-wheat amphiploids. Four H. marinum accessions were used as parents to produce amphiploids with Chinese Spring wheat, and their responses to hypoxic and 200 mM NaCl were evaluated. Relative growth rate (RGR) in the hypoxic-saline treatment was better maintained in the amphiploids (58–71% of controls) than in wheat (56% of control), but the amphiploids were more affected than H. marinum (68–97% of controls). In hypoxic-saline conditions, leaf Na+ concentrations in the amphiploids were lower than in wheat (30–41% lower) but were 39–47% higher than in the H. marinum parents. A strong barrier to radial oxygen loss formed in basal root zones under hypoxic conditions in two H. marinum accessions; this barrier was moderate in the amphiploids, absent in wheat, and was weaker for the hypoxic-saline treatment. Porosity of adventitious roots increased with the hypoxic treatments; values were 24–38% in H. marinum, 16–27% in the amphiploids and 16% in wheat. Overall, the amphiploids showed greater salt and waterlogging tolerances than wheat, demonstrating the expression of relevant traits from H. marinum in the amphiploids.

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Evaluation of root porosity and radial oxygen loss of disomic addition lines of Hordeum marinum in wheat

Functional Plant Biology ◽

10.1071/fp16272 ◽

2017 ◽

Vol 44 (4) ◽

pp. 400 ◽

Cited By ~ 7

Author(s):

Dennis Konnerup ◽

A. l. Imran Malik ◽

A. K. M. R. Islam ◽

Timothy David Colmer

Keyword(s):

Triticum Aestivum L ◽

Radial Oxygen Loss ◽

Waterlogging Tolerance ◽

Root Volume ◽

Root Porosity ◽

Oxygen Loss ◽

Chromosome Addition ◽

Disomic Addition Lines ◽

Gas Volume ◽

Root Aeration

Hordeum marinum Huds. is a waterlogging-tolerant wild relative of wheat (Triticum aestivum L.). Greater root porosity (gas volume per root volume) and formation of a barrier to reduce root radial O2 loss (ROL) contribute to the waterlogging tolerance of H. marinum and these traits are evident in some H. marinum–wheat amphiploids. We evaluated root porosity, ROL patterns and tolerance to hypoxic stagnant conditions for 10 various H. marinum (two accessions) disomic chromosome addition (DA) lines in wheat (two varieties), produced from two H. marinum–wheat amphiploids and their recurrent wheat parents. None of the DA lines had a barrier to ROL or higher root porosity than the wheat parents. Lack of a root ROL barrier in the six DA lines for H. marinum accession H21 in Chinese Spring (CS) wheat indicates that the gene(s) for this trait do not reside on one of these six chromosomes; unfortunately, chromosome 3 of H. marinum has not been isolated in CS. Unlike the H21–CS amphiploid, which formed a partial ROL barrier in roots, the H90–Westonia amphiploid and the four derived DA lines available did not. The unaltered root aeration traits in the available DA lines challenge the strategy of using H. marinum as a donor of these traits to wheat.

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A ‘Chinese Spring’ wheat (Triticum aestivum L.) bacterial artificial chromosome library and its use in the isolation of SSR markers for targeted genome regions

Theoretical and Applied Genetics ◽

10.1007/s00122-005-0077-1 ◽

2005 ◽

Vol 111 (8) ◽

pp. 1489-1494 ◽

Cited By ~ 15

Author(s):

B. Shen ◽

D.M. Wang ◽

C.L. McIntyre ◽

C.J. Liu

Keyword(s):

Triticum Aestivum ◽

Bacterial Artificial Chromosome ◽

Ssr Markers ◽

Spring Wheat ◽

Bacterial Artificial Chromosome Library ◽

Chinese Spring ◽

Artificial Chromosome ◽

Triticum Aestivum L ◽

Chinese Spring Wheat

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Chinese spring wheat (Triticum aestivum L.) chloroplast genome: Complete sequence and contig clones

Plant Molecular Biology Reporter ◽

10.1007/bf02823995 ◽

2000 ◽

Vol 18 (3) ◽

pp. 243-253 ◽

Cited By ~ 39

Author(s):

Yasunari Ogihara ◽

Kazuriho Isono ◽

Toshio Kojima ◽

Akira Endo ◽

Mitsumasa Hanaoka ◽

...

Keyword(s):

Triticum Aestivum ◽

Chloroplast Genome ◽

Spring Wheat ◽

Chinese Spring ◽

Triticum Aestivum L ◽

Complete Sequence ◽

Chinese Spring Wheat

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Anatomical Adaptive Strategies to Flooding and Rhizosphere Oxidation in Mangrove Seedlings

Australian Journal of Botany ◽

10.1071/bt9960297 ◽

1996 ◽

Vol 44 (3) ◽

pp. 297 ◽

Cited By ~ 71

Author(s):

T Youssef ◽

P Saenger

Keyword(s):

Root Surface ◽

Limited Information ◽

Ground Tissue ◽

Radial Oxygen Loss ◽

Waterlogging Tolerance ◽

Mangrove Species ◽

Root Porosity ◽

Oxygen Loss ◽

Diffusive Resistance ◽

Mangrove Seedlings

Limited information exists on the relation between the capacity of mangrove seedlings to oxidise the rhizosphere and their differential waterlogging tolerance. Laboratory experiments were conducted to estimate radial oxygen loss (ROL) by the entire root, the area of oxidising sites (AOS) on the root surface, root porosity (POR), and the internal diffusive resistance in the ground tissue of seedlings of six mangrove species that show a differential response to flooding. Radial oxygen loss was extremely low in all viviparous seedlings (0.7-1.5 μmol O2 per cm2AOS per day). Differential tolerance of species coincided with the degree of porosity (14.8-45.7%) and the ability of seedlings to develop barriers to oxygen leakage on the root surface. The percentage area of lacunae in the ground tissue of seedlings of the four viviparous species revealed a constriction of the air flow path at the hypocotyl junction. These findings suggest that: (i) the differential tolerance to waterlogging in mangrove seedlings is not simply based on their ability to oxidise the rhizosphere; (ii) the high diffusive resistance in the hypocotyl junction is likely to affect root aeration when the plant's access to air is limited by partial or total submergence; and (iii) waterlogging tolerance is probably a function of the strategy by which roots conserve oxygen to maintain aerobic metabolism for longer periods during submergence. Implications of these findings in seedlings are discussed in relation to other anatomical and morphological adaptations to waterlogging in mature mangroves.

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Genome-Wide Analysis of Microsatellite Markers Based on Sequenced Database in Chinese Spring Wheat (Triticum aestivum L.)

PLoS ONE ◽

10.1371/journal.pone.0141540 ◽

2015 ◽

Vol 10 (11) ◽

pp. e0141540 ◽

Cited By ~ 20

Author(s):

Bin Han ◽

Changbiao Wang ◽

Zhaohui Tang ◽

Yongkang Ren ◽

Yali Li ◽

...

Keyword(s):

Triticum Aestivum ◽

Microsatellite Markers ◽

Spring Wheat ◽

Chinese Spring ◽

Triticum Aestivum L ◽

Genome Wide Analysis ◽

Genome Wide ◽

Chinese Spring Wheat

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Control of alien gene expression for aluminum tolerance in wheat

Genome ◽

10.1139/g90-002 ◽

1990 ◽

Vol 33 (1) ◽

pp. 9-12 ◽

Cited By ~ 18

Author(s):

J. P. Gustafson ◽

K. Ross

Keyword(s):

Gene Expression ◽

Triticum Aestivum ◽

Chinese Spring ◽

Aluminum Tolerance ◽

Wheat Chromosome ◽

Triticum Aestivum L ◽

D Genome ◽

Chinese Spring Wheat ◽

Secale Cereale L ◽

Alien Gene

The expression of aluminum tolerance from rye (Secale cereale L.) when present in a wheat (Triticum aestivum L. em. Thell.) background has been observed to be much lower than that in rye itself. By crossing each of the ditelocentric lines of 'Chinese Spring' wheat with a tolerant rye, the effects of the presence or absence of each arm of wheat on the expression of rye aluminum tolerance could be established. Of 42 wheat chromosome arms, 18 affected the expression of rye aluminum tolerance. Tolerance was increased over that observed in the euploid wheat–rye hybrid when arms 4AL, 5AL, 6AL, 7BS, 7BL, and 3DS were absent. Tolerance was reduced when arms 2AL, 5AS, 6BS, 1DS, 1DL, 2DL, 4DL, 5DS, 5DL, 6DL, 7DS, and 7DL were absent. Thus, the control of aluminum tolerance expression from rye in a wheat background was evidently under the influence of genes located on a number of wheat chromosome arms, with a few arms tending to enhance expression and many others tending to reduce it. In fact, 5AS of 'Chinese Spring' enhances expression, while 5AL suppresses it. The D genome of bread wheat appears to have the most pronounced effect on the expression of rye aluminum tolerance.Key words: rye, activator genes, suppressor genes, alien manipulation.

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CHROMOSOMES OF CHINESE SPRING WHEAT CARRYING GENES FOR CROSSABILITY WITH BETZES BARLEY

Canadian Journal of Genetics and Cytology ◽

10.1139/g82-024 ◽

1982 ◽

Vol 24 (2) ◽

pp. 227-233 ◽

Cited By ~ 31

Author(s):

George Fedak ◽

Perry Y. Jui

Keyword(s):

Triticum Aestivum ◽

Hordeum Vulgare ◽

Chinese Spring ◽

Seed Set ◽

Triticum Aestivum L ◽

Chromosome Substitution ◽

Substitution Lines ◽

Hordeum Vulgare L ◽

Chromosome Substitution Lines ◽

Chinese Spring Wheat

Chromosome substitution lines of the variety Hope in Chinese Spring (Triticum aestivum L.) were crossed onto Betzes barley (Hordeum vulgare L. emend. Lam.). Three substitution lines of Hope involving chromosomes 5A, 5B, 5D gave no seed-set indicating that their counterparts in Chinese Spring were responsible for crossability with barley and that they function in complementary fashion. Other chromosomes of Hope had minor effects on crossability with barley.

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