Physiological and Transcriptomic Characterization of Sea-Wheatgrass-Derived Waterlogging Tolerance in Wheat

Waterlogging, causing hypoxia stress and nitrogen depletion in the rhizosphere, has been an increasing threat to wheat production. We developed a wheat–sea wheatgrass (SWG) amphiploid showing superior tolerance to waterlogging and low nitrogen. Validated in deoxygenated agar medium for three weeks, hypoxia stress reduced the dry matter of the wheat parent by 40% but had little effect on the growth of the amphiploid. To understand the underlying mechanisms, we comparatively analyzed the wheat–SWG amphiploid and its wheat parent grown in aerated and hypoxic solutions for physiological traits and root transcriptomes. Compared with its wheat parent, the amphiploid showed less magnitude in forming root porosity and barrier to radial oxygen loss, two important mechanisms for internal O2 movement to the apex, and downregulation of genes for ethylene, lignin, and reactive oxygen species. In another aspect, however, hypoxia stress upregulated the nitrate assimilation/reduction pathway in amphiploid and induced accumulation of nitric oxide, a byproduct of nitrate reduction, in its root tips, and the amphiploid maintained much higher metabolic activity in its root system compared with its wheat parent. Taken together, our research suggested that enhanced nitrate assimilation and reduction and accumulation of nitric oxide play important roles in the SWG-derived waterlogging tolerance.

Effects of Dissolved O2 and Fe Availability on Growth, Morphology, Aerenchyma Formation and Radial Oxygen Loss of Canna indica L. and Heliconia psittacorum L.f.

In constructed wetlands (CWs), plants are usually affected by low O2 levels. Under such conditions, most soluble iron is reduced to ferrous (Fe2+) which is highly soluble, and toxic to plants as well. As a consequence of excessive ferrous iron with low O2 supply, plant growth is reduced, leading to declining nutrient removal efficiency. This study was conducted to determine the effects of different dissolved oxygen levels (normoxia and hypoxia) with Fe supplied on growth, morphology, and root anatomy of two wetland plants (Canna indica and Heliconia psittacorum). The plants were grown on a nutrient solution modified from Smart and Barko (1985) under normoxic and hypoxic conditions. All plants were grown in greenhouse conditions for 42 days. Plant growth rates and biomass accumulation were drastically reduced under hypoxia while leaf number was not affected. Under hypoxia, root diameter and root porosity also increased in C. indica, whereas H. psittacorum had greater aerenchyma formation. Moreover, C. indica showed adaptive traits to cope with hypoxia and Fe stress by increasing radial oxygen loss (ROL), releasing O2 to the rhizosphere to resist toxic effects of ferrous iron under hypoxia. In contrast, H. psittacorum had no ROL under hypoxia. Moreover, the plants showed leaf chlorosis, leaf roll, and root rotting. Hence, it is suggested that C. indica could have better performance than H. psittacorum to treat wastewater in CWs as this species can adapt to hypoxic conditions and releases O2 into rhizosphere which improves dissolved oxygen (DO) in the wastewater. Keywords: Aerenchyma, Dissolved oxygen, Iron, Root porosity, Wetland emergent plant

Effect of the Process Atmosphere Composition on Alloy 718 Produced by Laser Powder Bed Fusion

The detrimental effect of nitrogen and oxygen when it comes to the precipitation of the strengthening γ’’ and γ’ phases in Alloy 718 is well-known from traditional manufacturing. Hence, the influence of the two processing atmospheres, namely argon and nitrogen, during the laser powder bed fusion (L-PBF) of Alloy 718 parts was studied. Regardless of the gas type, considerable losses of both oxygen of about 150 ppm O2 (≈30%) and nitrogen on the level of around 400 ppm N2 (≈25%) were measured in comparison to the feedstock powder. The utilization of nitrogen as processing atmosphere led to a slightly higher nitrogen content in the as-built material—about 50 ppm—compared to the argon atmosphere. The presence of the stable nitrides and Al-rich oxides observed in the as-built material was related to the transfer of these inclusions from the nitrogen atomized powder feedstock to the components. This was confirmed by dedicated analysis of the powder feedstock and supported by thermodynamic and kinetic calculations. Rapid cooling rates were held responsible for the limited nitrogen pick-up. Oxide dissociation during laser–powder interaction, metal vaporization followed by oxidation and spatter generation, and their removal by processing atmosphere are the factors describing an important oxygen loss during L-PBF. In addition, the reduction of the oxygen level in the process atmosphere from 500 to 50 ppm resulted in the reduction in the oxygen level in as-built component by about 5%.

Atmospheric Loss of Atomic Oxygen during Proton Aurorae on Mars

Abstract— For the first time, the calculations of the penetration of protons of the undisturbed solar wind into the daytime atmosphere of Mars due to charge exchange in the extended hydrogen corona (Shematovich et al., 2021) are used allowing us to determine self-consistently the sources of suprathermal oxygen atoms, as well as their kinetics and transport. An additional source of hot oxygen atoms—collisions accompanied by the momentum and energy transfer from the flux of precipitating high-energy hydrogen atoms to atomic oxygen in the upper atmosphere of Mars—was included in the Boltzmann kinetic equation, which was solved with the Monte-Carlo kinetic model. As a result, the population of the hot oxygen corona of Mars has been estimated; and it has been shown that the proton aurorae are accompanied by the atmospheric loss of atomic oxygen, which is evaluated within a range of (3.5–5.8) × 107 cm–2 s–1. It has been shown that the exosphere becomes populated with a substantial amount of suprathermal oxygen atoms with kinetic energies up to the escape energy, 2 eV. The atomic oxygen loss rate caused by a sporadic source in the Martian atmosphere—the precipitation of energetic neutral atoms of hydrogen (H‑ENAs) during proton aurorae at Mars—was estimated by the self-consistent calculations according to a set of the Monte-Carlo kinetic models. These values turned out be comparable to the atomic oxygen loss supported by a regular source—the exothermic photochemical reactions (Groeller et al., 2014; Jakosky et al., 2018). It is currently supposed that the atmospheric loss of Mars due to the impact of the solar wind plasma and, in particular, the fluxes of precipitating high-energy protons and hydrogen atoms during solar flares and coronal mass ejections may play an important role in the loss of the neutral atmosphere on astronomic time scales (Jakosky et al., 2018).