scholarly journals Overexpression of GSK3-like Kinase 5 (OsGSK5) in rice (Oryza sativa) enhances salinity tolerance in part via preferential carbon allocation to root starch

2017 ◽  
Vol 44 (7) ◽  
pp. 705 ◽  
Author(s):  
Maysaya Thitisaksakul ◽  
Maria C. Arias ◽  
Shaoyun Dong ◽  
Diane M. Beckles
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Salinity Tolerance ◽  
Carbon Allocation ◽  
Oryza Sativa L ◽  
Rice Seedling ◽  
Shoot Biomass ◽  
Salt Shock ◽  
Seedling Roots ◽  
Root Starch

Rice (Oryza sativa L.) is very sensitive to soil salinity. To identify endogenous mechanisms that may help rice to better survive salt stress, we studied a rice GSK3-like isoform (OsGSK5), an orthologue of a Medicago GSK3 previously shown to enhance salinity tolerance in Arabidopsis by altering carbohydrate metabolism. We wanted to determine whether OsGSK5 functions similarly in rice. OsGSK5 was cloned and sequence, expression, evolutionary and functional analyses were conducted. OsGSK5 was expressed highest in rice seedling roots and was both salt and sugar starvation inducible in this tissue. A short-term salt-shock (150 mM) activated OsGSK5, whereas moderate (50 mM) salinity over the same period repressed the transcript. OsGSK5 response to salinity was due to an ionic effect since it was unaffected by polyethylene glycol. We engineered a rice line with 3.5-fold higher OsGSK5 transcript, which better tolerated cultivation on saline soils (EC = 8 and 10 dS m–2). This line produced more panicles and leaves, and a higher shoot biomass under high salt stress than the control genotypes. Whole-plant 14C-tracing and correlative analysis of OsGSK5 transcript with eco-physiological assessments pointed to the accelerated allocation of carbon to the root and its deposition as starch, as part of the tolerance mechanism.

Physiological characterisation and fine mapping of a salt-tolerant mutant in rice (Oryza sativa)

2015 ◽  
Vol 42 (11) ◽  
pp. 1026 ◽  
Author(s):  
Ping Deng ◽  
Dan Jiang ◽  
Yanmin Dong ◽  
Xingyu Shi ◽  
Wen Jing ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Oryza Sativa L ◽  
Recessive Gene ◽  
Nacl Stress ◽  
Shoot Biomass ◽  
Salt Tolerant ◽  
Candidate Locus ◽  
F2 Population

Salt-tolerant mutants are valuable resources for basic and applied research on plant salt tolerance. Here, we report the isolation and characterisation of a salt-tolerant rice (Oryza sativa L.) mutant. This mutant was identified from an ethyl methanesulfonate-induced Nipponbare mutant library, designated as rice salt tolerant 1 (rst1). The rst1 mutant was tolerant to salt stress and showed significantly higher shoot biomass and chlorophyll content, but lower lipid peroxidation and electrolyte leakage under NaCl stress. The improved salt tolerance of this mutant may be due mainly to its enhanced ability to restrict Na+ accumulation in shoots under salt stress conditions. Genetic analysis indicated that the salt tolerance of the rst1 mutant was controlled by a single recessive gene. Quantitative trait locus (QTL) mapping for salt tolerance was performed using an F2 population of rst1 × Peiai 64. Two QTLs were detected, in which the locus on chromosome 6 was determined to be the candidate locus of the rst1 gene. The rst1 locus was subsequently shown to reside within a 270.4-kb region defined by the markers IM29432 and IM29702. This result will be useful for map-based cloning of the rst1 gene and for marker-assisted breeding for salt tolerance in rice.

scholarly journals Enhancement of the Aroma Compound 2-Acetyl-1-pyrroline in Thai Jasmine Rice (Oryza sativa) by Rhizobacteria under Salt Stress

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1065
Author(s):  
Kawiporn Chinachanta ◽  
Arawan Shutsrirung ◽  
Laetitia Herrmann ◽  
Didier Lesueur ◽  
Wasu Pathom-aree
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Oryza Sativa L ◽  
Plant Growth Promoting Rhizobacteria ◽  
Soil Salinization ◽  
Rice Seedling ◽  
Aroma Compound ◽  
Maximum Enhancement ◽  
Plant Growth Promoting ◽  
Jasmine Rice

Thai jasmine rice (Oryza sativa L. KDML105), particularly from inland salt-affected areas in Thailand, is both domestically and globally valued for its unique aroma and high grain quality. The key aroma compound, 2-acetyl-1-pyrroline (2AP), has undergone a gradual degradation due to anthropogenic soil salinization driven by excessive chemical input and climate change. Here, we propose a cheaper and an ecofriendly solution to improve the 2AP levels, based on the application of plant growth-promoting rhizobacteria (PGPR). In the present study, nine PGPR isolates from rice rhizosphere were investigated for the 2AP production in liquid culture and the promotion potential for 2AP content in KDML105 rice seedlings under four NaCl concentrations (0, 50, 100, and 150 mM NaCl). The inoculation of 2AP-producing rhizobacteria resulted in an increase in 2AP content in rice seedling leaves with the maximum enhancement from Sinomonas sp. ORF15-23 at 50 mM NaCl (19.6 µg·kg−1), corresponding to a 90.2% increase as compared to the control. Scanning electron microscopy confirmed the colonization of Sinomonas sp. ORF15-23 in the roots of salinity-stressed KDML105 seedlings. Our results provide evidence that Sinomonas sp. ORF15-23 could be a promising PGPR isolate in promoting aroma level of Thai jasmine rice KDML105 under salt stress.

High-density linkage mapping for agronomic and physiological traits of rice (Oryza sativa L.) under reproductive-stage salt stress

2021 ◽  
Vol 100 (2) ◽  
Author(s):  
Mostafa Ahmadizadeh ◽  
Nadali Babaeian-Jelodar ◽  
Ghasem Mohammadi-Nejad ◽  
Nadali Bagheri ◽  
Rakesh Kumar Singh
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Linkage Mapping ◽  
Oryza Sativa L ◽  
High Density ◽  
Reproductive Stage ◽  
Physiological Traits

Silicon alleviates salt stress-induced potassium deficiency by promoting potassium uptake and translocation in rice (Oryza sativa L.)

2021 ◽  
Vol 258-259 ◽  
pp. 153379
Author(s):  
Guochao Yan ◽  
Xiaoping Fan ◽  
Wanning Zheng ◽  
Zixiang Gao ◽  
Chang Yin ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Oryza Sativa L ◽  
Potassium Deficiency ◽  
Potassium Uptake

scholarly journals Screening of rice (Oryza sativa L.) for salinity tolerance at seedling stage under hydroponic condition

2020 ◽  
Vol 8 (4) ◽  
pp. 1776-1779
Author(s):  
Anuj Kumar ◽  
DK Dwivedi ◽  
Pratibha Yadav ◽  
Rahul Kumar ◽  
Preeti Kumari ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salinity Tolerance ◽  
Oryza Sativa L ◽  
Seedling Stage

scholarly journals Response of Iranian Rice Recombinant Inbred Lines (Oryza sativa L.) to Salt Stress in Seedling Stage

2019 ◽  
Vol 11 (29) ◽  
pp. 65-84
Author(s):  
Seyedeh Minoo Mirarab Razi ◽  
Reza Shirzadian-Khorramabad ◽  
Hossein Sabouri ◽  
Babak Rabiei ◽  
Hossein Hosseini Moghadam ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Recombinant Inbred ◽  
Oryza Sativa L ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Seedling Stage
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