Isolation and Characterization of 1'-O-Abscisic Acid- β -D-Glucopyranoside From Vegetative Tomato Tissue

1981 ◽  
Vol 8 (6) ◽  
pp. 571 ◽  
Author(s):  
BR Loveys ◽  
BV Milborrow
Keyword(s):  
Abscisic Acid ◽  
Anomeric Proton ◽  
Major Constituent ◽  
Alkaline Solutions ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Tomato Plants ◽  
Isolation And Characterization ◽  
Oxidase Test

A new, polar metabolite of abscisic acid (ABA) has been isolated from an aqueous extract of tomato plants. The compound is acidic, soluble in butanol and slightly soluble in acidic ethyl acetate. It is a major constituent of the products formed from (�)-[2-14C]abscisic acid and it occurs naturally. The structure was determined by gas-liquid chromatography-mass spectrometry (g.l.c.-m.s.) and nuclear magnetic resonance (n.m.r.) spectrometry as the 1'-O-β-D-giucopyrairoside of abscisic acid (ABAGS). The glucosyl moiety was identified by g.l.c.-m.s. and a glucose oxidase test, the ABA by n.m.r. and g.l.c. Tests with α- and β-glucosidase proved inconclusive, but the signal of the anomeric proton in the �H n.m.r. spectrum showed the 8 Hz coupling of a β-glucoside. ABAGS can be methylated with redistilled diazomethane and, like the glucose ester of ABA (ABAGE), it is unstable in alkaline solutions.

Seasonal variation of abscisic acid in the dormant shoots of Douglas-fir

1979 ◽  
Vol 57 (5) ◽  
pp. 534-538 ◽  
Author(s):  
Joe E. Webber ◽  
Murray L. Laver ◽  
Joe B. Zaerr ◽  
Denis P. Lavender
Keyword(s):  
Abscisic Acid ◽  
Liquid Chromatography ◽  
Seasonal Variation ◽  
Internal Standard ◽  
Close Correlation ◽  
Douglas Fir ◽  
Gas Liquid Chromatography ◽  
Bud Burst ◽  
Liquid Chromatography Mass Spectrometry ◽  
Layer Chromatography

The occurrence of abscisic acid (ABA) in the dormant shoots of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) was confirmed by bioassay, thin-layer chromatography, gas–liquid chromatography, and gas–liquid chromatography – mass spectrometry. Seasonal variation of ABA in the buds, leaves, and stems was then determined using 2-trans-ABA as an internal standard. Concentrations of ABA were highest in the autumn for buds (2.1 μg/g) and needles (0.79 μg/g) and highest in January for stems (0.34 μg/g). The lowest concentrations for all tissues were in February and March, before bud burst. Close correlation of levels of ABA with previously measured physiological evidence of growth and metabolic activity suggests a possible role in the dormancy cycle of Douglas-fir.

scholarly journals Gas-Liquid Chromatography–Mass Spectrometry Characterization of Polychlorinated Biphenyls (Aroclors) and 36Cl-Labeling of Aroclors 1248 and 1254

1971 ◽  
Vol 54 (4) ◽  
pp. 801-807
Author(s):  
David L Stalling ◽  
James N Huckins
Keyword(s):  
Mass Spectrometry ◽  
Aquatic Organisms ◽  
Sample Introduction ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Radioactive Materials ◽  
Isomer Composition ◽  
Temperature Programmed ◽  
Uptake And Metabolism

Abstract The isomer composition of the Aroclor 1200 series was characterized by GLC-MS, using temperature programming and SE-30 support-coated, open-tubular capillary columns. A method is described for the preparation and purification of 36Cl-labeled Aroclors 1248 and 1254. Neutron irradiation of the commercial material was used to prepare the 36Cl-labeled material. Purification of the irradiated product was accomplished by silicic acid column chromatography. Yields of the purified product were between 63 and 99%, with no detectable alteration of the isomer composition; 10% of the 36C1 produced was associated with the unchanged PCB isomers and the remaining radioactivity was contained in polychlorinated terphenyls. The terphenyls were produced by irradiation polymerization, and they were easily separated from the PCB components. Mass spectrometry, utilizing either temperature programmed GLC or direct probe sample introduction, was used to characterize the irradiation products. The radioactive materials are being utilized in experiments to determine uptake and metabolism of PCB by aquatic organisms.

Isolation and characterization of a barley mutant with abscisic-acid-insensitive stomata

Planta ◽  
1988 ◽  
Vol 173 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Ilva Raskin ◽  
Juanita A. R. Ladyman
Keyword(s):  
Abscisic Acid ◽  
Barley Mutant ◽  
Isolation And Characterization

The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana

1984 ◽  
Vol 61 (3) ◽  
pp. 377-383 ◽  
Author(s):  
M. Koornneef ◽  
G. Reuling ◽  
C. M. Karssen
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Isolation And Characterization

Characterization of abscisic acid and metabolites by combined liquid chromatography-mass spectrometry with ion-spray and plasma-spray ionization techniques

1992 ◽  
Vol 623 (2) ◽  
pp. 255-263 ◽  
Author(s):  
Lawrence R. Hogge ◽  
Garth D. Abrams ◽  
Suzanne R. Abrams ◽  
Pierre Thibault ◽  
Steven Pleasance
Keyword(s):  
Mass Spectrometry ◽  
Abscisic Acid ◽  
Liquid Chromatography ◽  
Plasma Spray ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

scholarly journals Study on the biosynthesis and structure characterization of exopolysaccharide from Lactobacillus fermentum MC3

2018 ◽  
Vol 127 (1D) ◽  
pp. 13
Author(s):  
Trần Thị Ái Luyến ◽  
Đỗ Thị Bích Thủy ◽  
Trần Thị Văn Thi ◽  
Phan Thị Thu Huyền
Keyword(s):  
Cell Density ◽  
Culture Medium ◽  
Structure Characterization ◽  
Lactobacillus Fermentum ◽  
Molar Ratio ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Initial Cell ◽  
Initial Cell Density

<p><strong>Abstract: </strong>The effects of carbohydrate sources in various concentration (2%, 3%, 4%, 5%, 6%) and fermentation conditions (such as initial cell density, temperature, pH and incubation time) on EPS synthesis of <em>Lactobacillus fermentum </em>MC3 were also studied. The results showed that adding different sugars (including glucose, lactose and sucrose) to culture medium significantly increased the EPS production. In comparison with other concentrations, EPS amounts were maximized in the medium supplemented with 4% (w/v) of sugars. The outcome was the highest for glucose, which was 178.207 mg/L, the obtained figures for lactose and for sucrose were 148.614 mg/L and 152.272 mg/L respectively. The results indicated that EPS production by <em>L. fermentum </em>MC3 reached the maximum values in the medium supplemented with 4% (w/v) glucose at 40<sup>0</sup>C, pH 6.0, initial cell density of 10<sup>6</sup>CFU/ml for 48 h cultivation with amount of 200.728 mg/L. By methylation analysis and gas–liquid chromatography–mass spectrometry (GLC–MS), the exopolysaccharide was found to be composed of D-mannose: D-glucose: D-galactose in a molar ratio of 1 : 0.74 : 0.09.</p>

scholarly journals Isolation and Characterization of Six Abscisic Acid-Inducible Genes from Carrot Somatic Embryos

2004 ◽  
Vol 21 (4) ◽  
pp. 309-314 ◽  
Author(s):  
Hajime SHIOTA ◽  
Guangxiao YANG ◽  
Shihua SHEN ◽  
Chang-Ho EUN ◽  
Ken-ichi WATABE ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Somatic Embryos ◽  
Isolation And Characterization ◽  
Inducible Genes

scholarly journals Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci

1996 ◽  
Vol 10 (4) ◽  
pp. 655-661 ◽  
Author(s):  
Karen M. Leon-Kloosterziel ◽  
Marta Alvarez Gil ◽  
Gerda J. Ruijs ◽  
Steven E. Jacobsen ◽  
Neil E. Olszewski ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Arabidopsis Mutants ◽  
Isolation And Characterization

Multivariate characterization of spontaneously generated electrical signals evoked by electrical stimulation in abscisic acid mutant tomato plants

2021 ◽  
Author(s):  
Fábia Barbosa da Silva ◽  
Francynes da Conceição Oliveira Macedo ◽  
Diogo Capelin ◽  
Gabriel Silva Daneluzzi ◽  
Aldeir Ronaldo Silva ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Abscisic Acid ◽  
Tomato Plants ◽  
Electrical Signals
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