The interaction of fresh and frozen-thawed ram spermatozoa with oviducal epithelial cells in vitro

2000 ◽  
Vol 12 (6) ◽  
pp. 237 ◽  
Author(s):  
L. Gillan ◽  
G. Evans ◽  
W. M. C. Maxwell
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Small Proportion ◽  
Oestrous Cycle ◽  
Cell Monolayers ◽  
Oviduct Epithelial Cell ◽  
Epithelial Cell Monolayers ◽  
Oviduct Epithelial Cells

In order to investigate the interaction of fresh and frozen–thawed spermatozoa with oviduct epithelial cells, spermatozoa were co-incubated with ovine oviduct epithelial cell monolayers (OECM) derived from either complete oviducts, at any stage of the oestrous cycle (Experiments 1 and 2), or from different regions of the oviduct at different stages of the cycle (Experiment 3). Fresh and frozen—thawed spermatozoa displayed different patterns of binding to, and release from, the OECM. Frozen—thawed spermatozoa immediately bound to the complete oviduct OECM and were released after 2 h. A small proportion of fresh spermatozoa bound immediately, increasing to a maximum after 2 h, and were gradually released thereafter. When only the cells that were released from the OECM were observed by chlortetracycline staining in Experiment 2, it was found that the presence of an OECM increased the number of capacitated fresh spermatozoa while decreasing the number of capacitated frozen–thawed spermatozoa. Overall, the OECM advanced the membrane state of both types of spermatozoa from uncapacitated to acrosome-reacted. Fresh and frozen—thawed spermatozoa bound to OECM derived from the cells of the isthmus and the ampulla in similar proportions. However, more spermatozoa were capacitated when incubated with OECM derived from isthmic rather than ampullary cells. Higher proportions of fresh spermatozoa bound to, and were acrosome-reacted following incubation with OECM derived from post- rather than pre-ovulatory tracts. Such differences were not observed for frozen—thawed spermatozoa. The findings reported in this study show that fresh and frozen—thawed spermatozoa behave differently when in contact with oviduct cells in vitro. This may be a consequence of the more advanced membrane state of the frozen spermatozoa upon thawing.

Characterization of bovine oviduct epithelial cell monolayers cultured under serum-free conditions

1995 ◽  
Vol 31 (9) ◽  
pp. 664-670 ◽  
Author(s):  
A. Van Langendonckt ◽  
A. Vansteenbrugge ◽  
C. Dessy-Doize ◽  
J. E. Flechon ◽  
G. Charpigny ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Serum Free ◽  
Cell Monolayers ◽  
Oviduct Epithelial Cell ◽  
Epithelial Cell Monolayers ◽  
Serum Free Conditions

Evaluating an in vitro culture system of bovine uterine and oviduct epithelial cells for subsequent embryo co-culture

1992 ◽  
Vol 4 (5) ◽  
pp. 573 ◽  
Author(s):  
JK Thibodeaux ◽  
MW Myers ◽  
LL Goodeaux ◽  
Y Menezo ◽  
JD Roussel ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Growth Rates ◽  
Culture System ◽  
Culture Medium ◽  
Growth Patterns ◽  
Oviduct Epithelial Cells

Three experiments were conducted to evaluate the effects of culture medium and incubation temperature on bovine uterine and oviduct epithelial cell growth, so that the most efficient combination could then be used to develop a co-culture system for bovine embryos. In the first experiment, uterine and oviduct epithelial cells at either the second or third subpassage were incubated for 8 days at 37 degrees C with 5% CO2 in Tissue Culture Medium-199, CMRL-1066, Minimal Essential Medium, Menezo's B2 or Ham's F-12 medium. In addition to plotting growth curves of cell populations, the cell cycle was monitored for 8 days by flow cytometry. Uterine and oviduct epithelial cells incubated in CMRL-1066 exhibited the highest growth rates during the 8-day culture period. However, there were no differences in cell cycle analysis among treatment groups during the incubation period. In the second experiment, CMRL-1066 medium was used to evaluate growth and proliferation of uterine and oviduct epithelial cells incubated at 37 degrees C or 39 degrees C; temperature had no significant effect on growth rates or proliferation rates for either uterine or oviduct cells during the 8-day incubation. In the third experiment, the more promising culture media for epithelial cell culture studies were chosen for in vitro maturation and subsequent in vitro fertilization (IVF) of bovine oocytes. Early cleavage-stage embryos produced by IVF procedures were subsequently cultured in vitro for 7 days in medium alone or with oviduct epithelial cells. In this study, the culture medium did not influence fertilization or cleavage rates. However, more embryos co-cultured with oviduct epithelial cells were considered viable after 7 days of incubation compared with embryos incubated in medium alone. These results indicate that various incubation conditions can influence the growth of bovine uterine and oviduct epithelial cells in vitro. However, in spite of changes in cell growth patterns, there does not appear to be a change in their embryotropic capabilities in vitro.

scholarly journals Impact of Hydroxychloroquine-Loaded Polyurethane Intravaginal Rings on Lactobacilli

2015 ◽  
Vol 59 (12) ◽  
pp. 7680-7686 ◽  
Author(s):  
Yannick Leandre Traore ◽  
Yufei Chen ◽  
Anne-Marie Bernier ◽  
Emmanuel A. Ho
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Female Genital Tract ◽  
Vaginal Epithelial Cell ◽  
Content Type ◽  
Cell Monolayers ◽  
Intravaginal Rings ◽  
Vaginal Epithelial Cells ◽  
Epithelial Cell Monolayers ◽  
The Impact

ABSTRACTThe use of polymeric devices for controlled sustained delivery of drugs is a promising approach for the prevention of HIV-1 infection. Unfortunately, certain microbicides, when topically applied vaginally, may be cytotoxic to vaginal epithelial cells and the protective microflora present within the female genital tract. In this study, we evaluated the impact of hydroxychloroquine (HCQ)-loaded, reservoir-type, polyurethane intravaginal rings (IVRs) on the growth ofLactobacillus crispatusandLactobacillus jenseniiand on the viability of vaginal and ectocervical epithelial cells. The IVRs were fabricated using hot-melt injection molding and were capable of providing controlled release of HCQ for 24 days, with mean daily release rates of 17.01 ± 3.6 μg/ml in sodium acetate buffer (pH 4) and 29.45 ± 4.84 μg/ml in MRS broth (pH 6.2). Drug-free IVRs and the released HCQ had no significant effects on bacterial growth or the viability of vaginal or ectocervical epithelial cells. Furthermore, there was no significant impact on the integrity of vaginal epithelial cell monolayers, in comparison with controls, as measured by transepithelial electrical resistance. Overall, this is the first study to evaluate the effects of HCQ-loaded IVRs on the growth of vaginal flora and the integrity of vaginal epithelial cell monolayers.

scholarly journals Human sperm function in co-culture with human, macaque or bovine oviduct epithelial cell monolayers

1998 ◽  
Vol 13 (10) ◽  
pp. 2797-2804 ◽  
Author(s):  
J.E. Ellington ◽  
A.E. Jones ◽  
C.M. Davitt ◽  
C.S. Schneider ◽  
R.S. Brisbois ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Human Sperm ◽  
Sperm Function ◽  
Cell Monolayers ◽  
Oviduct Epithelial Cell ◽  
Epithelial Cell Monolayers

scholarly journals Transient active osmotic swelling of epithelium upon curvature induction

2020 ◽  
Author(s):  
Caterina Tomba ◽  
Valeriy Luchnikov ◽  
Luca Barberi ◽  
Carles Blanch-Mercader ◽  
Aurélien Roux
Keyword(s):  
Epithelial Cell ◽  
Primary Response ◽  
Membrane Tension ◽  
Osmotic Swelling ◽  
Volume Increase ◽  
Cell Monolayers ◽  
Collective Response ◽  
Epithelial Cell Monolayers ◽  
Hypertonic Shock

Generation of tissue curvature is essential to morphogenesis. However, how cells adapt to changing curvature is still unknown because tools to dynamically control curvature in vitro are lacking. Here we developed self-rolling substrates to study how flat epithelial cell monolayers adapt to a rapid, anisotropic change of curvature. We show that the primary response is an active and transient osmotic swelling of cells. This cell volume increase is not observed on inducible wrinkled substrates, where concave and convex regions alternate each other over short distances, identifying swelling as a collective response to changes of curvature with persistent sign over large distances. It is triggered by a drop in membrane tension and actin depolymerization, perceived by cells as a hypertonic shock. Osmotic swelling restores tension while actin reorganizes, probably to comply with curvature. Epithelia are thus unique materials that transiently, actively swell while adapting to large curvature induction.

scholarly journals Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

2006 ◽  
Vol 188 (2) ◽  
pp. 205-213 ◽  
Author(s):  
S E Ulbrich ◽  
S Rehfeld ◽  
S Bauersachs ◽  
E Wolf ◽  
R Rottmayer ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Nitric Oxide Synthases ◽  
Oestrous Cycle ◽  
Inos Mrna ◽  
Female Reproduction ◽  
Specific Expression ◽  
Oviduct Epithelial Cells

Nitric oxide synthases (NOS) account for the endogenous production of nitric oxide (NO), a small and permeable bioreactive molecule. NO is known to act as a paracrine mediator during various processes associated with female reproduction. In the present study, the mRNA expression of the endothelial (eNOS) and inducible (iNOS) NO synthases were examined in bovine oviduct epithelial cells (BOEC) during the oestrous cycle. In addition, eNOS and iNOS mRNA and protein were localised by in situ hybridisation and immunocytochemistry respectively. Furthermore, the effects of exogenously applied oestradiol-17β and progesterone on NOS mRNA regulation were studied in a suspension culture of BOEC. The eNOS mRNA abundance was low around ovulation (day 0) and increased significantly until pro-oestrus (day 18) in the ampulla. Immunoreactive protein of eNOS was detected predominantly in endothelial cells as well as in secretory oviduct epithelial cells at pro-oestrus. The iNOS mRNA concentration was significantly reduced in the isthmus at pro-oestrus (day 18) and oestrus (day 0) compared with persistently high levels in the ampulla. By in situ hybridisation, specific iNOS transcripts were additionally demonstrated in the oviduct epithelium. Immunoreactive iNOS protein was localised in secretory epithelial cells as well as in the lamina muscularis. The in vitro stimulation showed that both NOS were stimulated by progesterone, but not by oestradiol-17β. The region-specific modulated expression of eNOS and iNOS provides evidence for an involvement of endogenously produced NO in the regulation of oviductal functions.

In vitro model of “wound healing” analyzed by laser scanning cytometry: Accelerated healing of epithelial cell monolayers in the presence of hyaluronate

Cytometry ◽  
2003 ◽  
Vol 53A (1) ◽  
pp. 1-8 ◽  
Author(s):  
Asifa S. Haider ◽  
Jerzy Grabarek ◽  
Ben Eng ◽  
Paulina Pedraza ◽  
Nicholas R. Ferreri ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Laser Scanning ◽  
In Vitro Model ◽  
Cell Monolayers ◽  
Laser Scanning Cytometry ◽  
Epithelial Cell Monolayers ◽  
Vitro Model
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