Identification and characterization of a gene coding a novel isoform of DEAD-box protein

2002 ◽  
Vol 14 (3) ◽  
pp. 185 ◽  
Author(s):  
Lanlan Yin ◽  
JianMin Li ◽  
Hu Zhu ◽  
Min Lin ◽  
Lijun Cheng ◽  
...  
Keyword(s):  
Human Testis ◽  
Microarray Hybridization ◽  
Chain Reaction ◽  
Dead Box ◽  
Gene Coding ◽  
Testis Cdna Library ◽  
Testis Cdna ◽  
Polymerase Chain ◽  
Identification And Characterization

A gene coding a novel isoform of DEAD-box protein named testicular DEAD-box protein (tDbp), presumably involved in testicular function, was identified and characterized. Testicular DEAD-box protein was cloned from a human testis cDNA library. The cDNA microarray hybridization showed that it was expressed at a higher level in adult testis than in embryo testis. Reverse transcription-polymerase chain reaction indicated that tDbp was specifically expressed in testis, but not in some other tissues.

scholarly journals Identification and characterization of Edwardsiella ictaluri from diseased Pangasius pangasius, cultured in Cirata Lake, Indonesia

2018 ◽  
Vol 19 (3) ◽  
pp. 816-822 ◽  
Author(s):  
MIRA MAWARDI ◽  
JAELANI JAELANI ◽  
ZAKKI ZAINUN ◽  
YUANI MUNDAYANA ◽  
BAIRD SAM CHILORA ◽  
...  
Keyword(s):  
Edwardsiella Ictaluri ◽  
Pcr Analysis ◽  
Nucleotide Sequencing ◽  
Infection Prevalence ◽  
Chain Reaction ◽  
Prevalence Level ◽  
Conventional Biochemical Test ◽  
Polymerase Chain ◽  
Identification And Characterization

Mawardi M, Jaelani, Zainun Z, Mundayana Y, Chilora BS, Hardi EH. 2018. Identification and characterization of Edwardsiella ictaluri from diseased Pangasius pangasius, cultured in Cirata Lake, Indonesia. Biodiversitas 19: 766-772. In January 2016, there were reported extensive mortalities of Pangasius pangasius cultured on cage, in Cirata Lake. Edwardsiella ictaluri is primarily recognized as a disease-causing pathogen in catfish species, causing an economically important bacterial infection hence affecting productivity of aquaculture enterprises in many regions across the E. ictaluri. The samples were 17 fishes collected from organs containing spleen, kidney, and liver. The total samples were 51 organs of fishes. The high bacteria infection prevalence level was found in kidney (29.41%). The gross pathological signs of sample of organs were abnormal sizes and abnormal color including white nodules. Bacteria were identified by conventional biochemical test, polymerase chain reaction (PCR) analysis and nucleotide sequencing. The results showed that P. pangasius cultured in cages in Cirata Lake is positively infected by E. ictaluri strain (accession number KF9071291). Other infection cases by Aeromonas sp. and Plesiomonas shigelloides as co-infection bacteria in P. pangasius were also found.

scholarly journals Identification and Characterization of Tomato Mutants Affected in the Rx-Mediated Resistance to PVX Isolates

2012 ◽  
Vol 25 (3) ◽  
pp. 341-354 ◽  
Author(s):  
Bénédicte Sturbois ◽  
Marie-Pierre Dubrana-Ourabah ◽  
Julie Gombert ◽  
Bertrand Lasseur ◽  
Audrey Macquet ◽  
...  
Keyword(s):  
Potato Virus X ◽  
Viral Capsid ◽  
Parental Line ◽  
Chain Reaction ◽  
Systemic Necrosis ◽  
Protein Elicitor ◽  
Polymerase Chain ◽  
Identification And Characterization ◽  
Avirulent Isolate

Five tomato mutants affected in the Rx-mediated resistance against Potato virus X (PVX) were identified by screening a mutagenized population derived from a transgenic, Rx1-expressing ‘Micro-Tom’ line. Contrary to their parental line, they failed to develop lethal systemic necrosis upon infection with the virulent PVX-KH2 isolate. Sequence analysis and quantitative reverse-transcription polymerase chain reaction experiments indicated that the mutants are not affected in the Rx1 transgene or in the Hsp90, RanGap1 and RanGap2, Rar1 and Sgt1 genes. Inoculation with the PVX-CP4 avirulent isolate demonstrated that the Rx1 resistance was still effective in the mutants. In contrast, the virulent PVX-KH2 isolate accumulation was readily detectable in all mutants, which could further be separated in two groups depending on their ability to restrict the accumulation of PVX-RR, a mutant affected at two key positions for Rx1 elicitor activity. Finally, transient expression of the viral capsid protein elicitor indicated that the various mutants have retained the ability to mount an Rx1-mediated hypersensitive response. Taken together, the results obtained are consistent with a modification of the specificity or intensity of the Rx1-mediated response. The five Micro-Tom mutants should provide very valuable resources for the identification of novel tomato genes affecting the functioning of the Rx gene.

scholarly journals Characterization of a new human apolipoprotein A-I Yame by direct sequencing of polymerase chain reaction-amplified DNA.

1991 ◽  
Vol 32 (8) ◽  
pp. 1275-1280
Author(s):  
Y Takada ◽  
J Sasaki ◽  
M Seki ◽  
S Ogata ◽  
Y Teranishi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Direct Sequencing ◽  
Chain Reaction ◽  
Human Apolipoprotein ◽  
Apolipoprotein A ◽  
Polymerase Chain

scholarly journals Characterization of Xanthomonas axonopodis pv. phaseoli isolates

2008 ◽  
Vol 34 (3) ◽  
pp. 228-231 ◽  
Author(s):  
Willian Mário de Carvalho Nunes ◽  
Maria Júlia Corazza ◽  
Silvana Aparecida Crestes Dias de Souza ◽  
Siu Mui Tsai ◽  
Eiko Eurya Kuramae
Keyword(s):  
Xanthomonas Campestris ◽  
Random Amplified Polymorphic Dna ◽  
Hypersensitive Reaction ◽  
Chain Reaction ◽  
Xanthomonas Axonopodis ◽  
Paulo State ◽  
Total Dna ◽  
Polymerase Chain ◽  
Bacterial Plant Pathogen

A simple, quick and easy protocol was standardized for extraction of total DNA of the bacteria Xanthomonas axonopodis pv. phaseoli. The DNA obtained by this method had high quality and the quantity was enough for the Random Amplified Polymorphic DNA (RAPD) reactions with random primers, and Polymerase Chain Reaction (PCR) with primers of the hypersensitivity and pathogenicity gene (hrp). The DNA obtained was free of contamination by proteins or carbohydrates. The ratio 260nm/380nm of the DNA extracted ranged from 1.7 to 1.8. The hrp gene cluster is required by bacterial plant pathogen to produce symptoms on susceptible hosts and hypersensitive reaction on resistant hosts. This gene has been found in different bacteria as well as in Xanthomonas campestris pv. vesicatoria (9). The primers RST21 and RST22 (9) were used to amplify the hrp gene of nine different isolates of Xanthomonas axonopodis pv. phaseoli from Botucatu, São Paulo State, Brazil, and one isolate, "Davis". PCR amplified products were obtained in all isolates pathogenic to beans.

Characterization of Giardia duodenalis by polymerase-chain-reaction fingerprinting

1998 ◽  
Vol 84 (9) ◽  
pp. 707-714 ◽  
Author(s):  
Wieger L. Homan ◽  
Margriet Gilsing ◽  
Hafida Bentala ◽  
Louis Limper ◽  
Frans van Knapen
Keyword(s):  
Polymerase Chain Reaction ◽  
Giardia Duodenalis ◽  
Chain Reaction ◽  
Polymerase Chain
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