397 DERIVATION OF NEURAL STEM CELLS FROM PORCINE EPIBLAST CELLS

2010 ◽  
Vol 22 (1) ◽  
pp. 355
Author(s):  
M. A. Rasmussen ◽  
V. J. Hall ◽  
P. Hyttel
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Neural Stem Cell ◽  
Bipolar Cells ◽  
Stem Cell Markers ◽  
High Density Culture ◽  
Cell Markers ◽  
Stem Cell Medium ◽  
Cell Medium

The use of neural stem cells (NSC) has gained increased attention as a means of treating neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. However, before regenerative treatment of humans can be undertaken, safety studies of NSC using animal models are required. The mouse has been the model of choice so far; however, testing in larger mammals such as the pig is essential. The aim of this study was to derive NSC from porcine epiblast cells and to analyze these cells using neural stem cell markers. A total of 47 epiblasts were isolated from E9 porcine embryos and grown on mouse embryonic fibroblast cells in a porcine embryonic stem cell medium. After 5 days, 23 outgrowth colonies had formed (49%). Based on morphology, 8 outgrowth colonies were selected and cut into 63 smaller pieces, which were transferred to MS5 stromal cells in a serum replacement medium, and after an additional 12 days, rosette structures had formed. These structures were transferred to Matrigel-coated dishes in a neural stem cell medium containing EGF and FGF. Under such conditions, bipolar cells containing large nuclei and several nucleoli grew out from the rosettes. The bipolar cells have been expanded for more than 8 passages without any change in morphology or growth rate, and upon high-density culture, the cells spontaneously form floating neurospheres. Stainings revealed that the cells expressed the neural stem cell markers Nestin (100%), Sox2 (100%), Pax6 (100%), and Vimentin (100%), as well as the proliferation marker Ki67 (54%). The same markers were found to be expressed in the lateral ventricles of the developing porcine brain, a location known to have high neurogenic activity. When growth factors were withdrawn from the culture medium, a higher proportion of TujI expressing cells were observed, especially when cells were cultured as neurospheres. We conclude that it is possible to derive presumptive NSC from porcine epiblast cells and that these express the same markers as reported for human NSC. Further studies are required to determine if the cells can be cultured long term and differentiate into various neuronal and glial cell types.

scholarly journals Expression of the Neural Stem Cell Markers NG2 and L1 in Human Angiomyolipoma: Are Angiomyolipomas Neoplasms of Stem Cells?

2007 ◽  
Vol 13 (3-4) ◽  
pp. 160-165 ◽  
Author(s):  
So Dug Lim ◽  
William Stallcup ◽  
Benjamin Lefkove ◽  
Baskaran Govindarajan ◽  
Kit Sing Au ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Markers ◽  
Cell Markers

scholarly journals The Neural Stem Cell Properties of PKD2L1+ Cerebrospinal Fluid-Contacting Neurons in vitro

2021 ◽  
Vol 15 ◽  
Author(s):  
Shuo Wang ◽  
Yuqi He ◽  
Huiqian Zhang ◽  
Li Chen ◽  
Liang Cao ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Cerebrospinal Fluid ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Neural Stem Cell ◽  
Immunofluorescence Staining ◽  
Stem Cell Markers ◽  
Cord Injury

Cerebrospinal fluid-touching neurons (CSF-cNs) exist in the region surrounding the central canal of the spinal cord, which locate in the adult neurogenic niche. Previous research showed that CSF-cNs expressed the molecular markers of immature neural cells in vivo. Here, we explored the potential of CSF-cNs as neural stem cell in intro. We first found that PKD2L1+ CSF-cNs, isolating by FACS using the molecular marker PKD2L1 of CSF-cNs, expressed neural stem cells markers like Nestin, Sox2, and GFAP by immunofluorescence staining. PKD2L1+ CSF-cNs were able to form neurospheres and passaged in vitro. Immunofluorescence staining showed that the neurospheres forming by PKD2L1+ CSF-cNs also expressed neural stem cell markers Nestin, Sox2 and GFAP. The neurospheres expressed proliferation markers Ki67 and PCNA by immunofluorescence staining, indicating that the neurospheres forming by PKD2L1+ CSF-cNs were proliferative. The neurospheres, forming by CSF-cNs, had the ability of differentiation into neurons, astrocytes, and oligodendrocytes. Collectively, our data suggested that PKD2L1+ CSF-cNs have the properties of neural stem cells in vitro and may provide a promising approach for the repair of spinal cord injury.

Stress resistant human embryonic stem cells as a potential source for the identification of novel cancer stem cell markers

2010 ◽  
Vol 289 (2) ◽  
pp. 208-216 ◽  
Author(s):  
Shaker A. Mousa ◽  
Thangirala Sudha ◽  
Evgeny Dyskin ◽  
Usawadee Dier ◽  
Christine Gallati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Cancer Stem Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Cancer Stem Cell Markers ◽  
Potential Source

scholarly journals P080 Therapeutical effect of urine-derived stem cells on DSS-induced chronic model of mice

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S175-S175
Author(s):  
X R Wu ◽  
C Zhou ◽  
H S Liu ◽  
L Xuan-hui ◽  
T Hu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Low Cost ◽  
Stem Cell Markers ◽  
Male Adult ◽  
Hematopoietic Stem ◽  
Cell Markers ◽  
Murine Colitis ◽  
Chronic Model

Abstract Background The application of stem cell therapy in the treatment of inflammatory bowel diseases (IBD) is limited because of the invasive approaches of stem cells. Urine-derived stem cells (USCs) were recently shown to have regenerative properties, which can be harvested in a safe, low-cost and non-invasive way. Methods Human USC were isolated and expanded from the urine of healthy male adult volunteers (n = 3, age arrange 24–30 years old). USC were characterised by cell surface marker expression profile and multipotent differentiation. In vivo therapeutic value of USC was assessed using murine colitis chronic model induced by dextran sulphate sodium (DSS). Results USC were positive for mesenchymal stem cell markers but were negative for hematopoietic stem cell markers. These cells differentiated into osteo-, adipo- and chondro-genic cell lineages. Systemic administration of USC significantly ameliorated the clinical and histopathological severity of colitis and increased the survival rate in chronic murine colitis model. Conclusion This study demonstrated that implantation of USC reduces inflammation in IBD rodent model, indicating that USC therapy serves as a potential cell-based therapeutic candidate for IBD.

scholarly journals Loss of postnatal quiescence of neural stem cells through mTOR activation upon genetic removal of cysteine string protein-α

2019 ◽  
Vol 116 (16) ◽  
pp. 8000-8009 ◽  
Author(s):  
Jose L. Nieto-González ◽  
Leonardo Gómez-Sánchez ◽  
Fabiola Mavillard ◽  
Pedro Linares-Clemente ◽  
María C. Rivero ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Neural Stem Cell ◽  
Molecular Mechanisms ◽  
Radial Glia ◽  
Brain Dysfunction ◽  
Mtor Signaling ◽  
Cysteine String Protein ◽  
Newborn Neurons

Neural stem cells continuously generate newborn neurons that integrate into and modify neural circuitry in the adult hippocampus. The molecular mechanisms that regulate or perturb neural stem cell proliferation and differentiation, however, remain poorly understood. Here, we have found that mouse hippocampal radial glia-like (RGL) neural stem cells express the synaptic cochaperone cysteine string protein-α (CSP-α). Remarkably, in CSP-α knockout mice, RGL stem cells lose quiescence postnatally and enter into a high-proliferation regime that increases the production of neural intermediate progenitor cells, thereby exhausting the hippocampal neural stem cell pool. In cell culture, stem cells in hippocampal neurospheres display alterations in proliferation for which hyperactivation of the mechanistic target of rapamycin (mTOR) signaling pathway is the primary cause of neurogenesis deregulation in the absence of CSP-α. In addition, RGL cells lose quiescence upon specific conditional targeting of CSP-α in adult neural stem cells. Our findings demonstrate an unanticipated cell-autonomic and circuit-independent disruption of postnatal neurogenesis in the absence of CSP-α and highlight a direct or indirect CSP-α/mTOR signaling interaction that may underlie molecular mechanisms of brain dysfunction and neurodegeneration.

scholarly journals Intracerebral implantation of human neural stem cells and motor recovery after stroke: multicentre prospective single-arm study (PISCES-2)

2020 ◽  
Vol 91 (4) ◽  
pp. 396-401 ◽  
Author(s):  
Keith W Muir ◽  
Diederik Bulters ◽  
Mark Willmot ◽  
Nikola Sprigg ◽  
Anand Dixit ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Multicentre Study ◽  
Neural Stem Cells ◽  
Neural Stem Cell ◽  
Upper Limb ◽  
Limb Movement ◽  
Human Neural Stem Cells ◽  
Human Neural Stem Cell ◽  
Cell Implantation

BackgroundHuman neural stem cell implantation may offer improved recovery from stroke. We investigated the feasibility of intracerebral implantation of the allogeneic human neural stem cell line CTX0E03 in the subacute—chronic recovery phase of stroke and potential measures of therapeutic response in a multicentre study.MethodsWe undertook a prospective, multicentre, single-arm, open-label study in adults aged >40 years with significant upper limb motor deficits 2–13 months after ischaemic stroke. 20 million cells were implanted by stereotaxic injection to the putamen ipsilateral to the cerebral infarct. The primary outcome was improvement by 2 or more points on the Action Research Arm Test (ARAT) subtest 2 at 3 months after implantation.FindingsTwenty-three patients underwent cell implantation at eight UK hospitals a median of 7 months after stroke. One of 23 participants improved by the prespecified ARAT subtest level at 3 months, and three participants at 6 and 12 months. Improvement in ARAT was seen only in those with residual upper limb movement at baseline. Transient procedural adverse effects were seen, but no cell-related adverse events occurred up to 12 months of follow-up. Two deaths were unrelated to trial procedures.InterpretationAdministration of human neural stem cells by intracerebral implantation is feasible in a multicentre study. Improvements in upper limb function occurred at 3, 6 and 12 months, but not in those with absent upper limb movement at baseline, suggesting a possible target population for future controlled trials.FundingReNeuron, Innovate UK (application no 32074-222145).Trial registration numberEudraCT Number: 2012-003482-18

Flow Cytometric Analysis of Neural Stem Cell Markers on Pediatric Brain Tumors

Neurosurgery ◽  
2005 ◽  
Vol 57 (2) ◽  
pp. 434-434
Author(s):  
Samuel Cheshier ◽  
Laurie E. Ailles ◽  
Michael Lim ◽  
Paul Laddis ◽  
Victor C.K. Tse ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Tumors ◽  
Neural Stem Cell ◽  
Flow Cytometric Analysis ◽  
Pediatric Brain Tumors ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Flow Cytometric ◽  
Pediatric Brain
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