Detection of the sexually transmissible genital mycoplasmas by polymerase chain reaction in women

Sexual Health ◽  
2011 ◽  
Vol 8 (3) ◽  
pp. 445 ◽  
Author(s):  
Vessela V. Ouzounova-Raykova ◽  
Rumyana Markovska ◽  
Gergana Mizgova ◽  
Ivan G. Mitov
Keyword(s):  
Polymerase Chain Reaction ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Chain Reaction ◽  
Clinical Disorders ◽  
Polymerase Chain ◽  
Asymptomatic Women ◽  
Genital Mycoplasmas

Background The role of Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium in the development of clinical disorders is still unclear. The aim of this study was to determine their prevalence in symptomatic and asymptomatic women. Methods: A total of 348 women were examined by applying polymerase chain reaction (PCR) methods. Results: The registered prevalence was as follows: U. urealyticum 14.66%; M. hominis 3.16%; and M. genitalium 0.29%. Co-infection was established in 11 swabs. Conclusions: This is the first study in Bulgaria for the detection of mycoplasmas by PCR. Our results demonstrate similar or lower values in comparison with other researchers and further investigations are needed.

Isolation of Ureaplasma urealyticum and Mycoplasma hominis From Public Toilet Bowls

1999 ◽  
Vol 20 (01) ◽  
pp. 66-68 ◽  
Author(s):  
Israel Potasman ◽  
Amnon Oren ◽  
Isaac Srugo
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Antigen Detection ◽  
Chain Reaction ◽  
Survival Assay ◽  
Polymerase Chain

Abstract The presence of Ureaplasma urealyticum, Mycoplasma hominis, and Chlamydia trachomatis was explored in 50 public restroom toilet bowls. We used culture, antigen detection, polymerase chain reaction, and survival assay. Five bowls (10%) were contaminated with at least one organism. U urealyticum was found in four bowls, M hominis in three, and C trachomatis in one. U urealyticum survived on the toilet rim for up to 2 hours.

scholarly journals Detection of mycoplasmas in urethral swabs from HIV-1 infected patients and control individuals using culture techniques and polymerase chain reaction

1998 ◽  
Vol 40 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Regina Ayr Florio da CUNHA ◽  
Kioko TAKEI ◽  
Adelaide José VAZ ◽  
Caio ROSENTHAL
Keyword(s):  
Polymerase Chain Reaction ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Species ◽  
Control Group ◽  
Chain Reaction ◽  
Culture Techniques ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Hiv 1

The objective of the present study was to determine the prevalence of certain mycoplasma species, i.e., Mycoplasma hominis, Ureaplasma urealyticum and Mycoplasma penetrans, in urethral swabs from HIV-1 infected patients compared to swabs from a control group. Mycoplasmas were detected by routine culture techniques and by the Polymerase Chain Reaction (PCR) technique, using 16SrRNA generic primers of conserved region and Mycoplasma penetrans specific primers. The positivity rates obtained with the two methods were comparable. Nevertheless, PCR was more sensitive, while the culture techniques allowed the quantification of the isolates. The results showed no significant difference (p < 0.05) in positivity rates between the methods used for mycoplasma detection.

scholarly journals Macrophage migration inhibitory factor may play a protective role in osteoarthritis

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Ming Liu ◽  
Zikun Xie ◽  
Guang Sun ◽  
Liujun Chen ◽  
Dake Qi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Protective Role ◽  
Macrophage Migration ◽  
Chain Reaction ◽  
Protein Levels ◽  
Tnf Α ◽  
Polymerase Chain

Abstract Background Osteoarthritis (OA) is the most prevalent form of arthritis and the major cause of disability and overall diminution of quality of life in the elderly population. Currently there is no cure for OA, partly due to the large gaps in our understanding of its underlying molecular and cellular mechanisms. Macrophage migration inhibitory factor (MIF) is a procytokine that mediates pleiotropic inflammatory effects in inflammatory diseases such as rheumatoid arthritis (RA) and ankylosing spondylitis (AS). However, data on the role of MIF in OA is limited with conflicting results. We undertook this study to investigate the role of MIF in OA by examining MIF genotype, mRNA expression, and protein levels in the Newfoundland Osteoarthritis Study. Methods One hundred nineteen end-stage knee/hip OA patients, 16 RA patients, and 113 healthy controls were included in the study. Two polymorphisms in the MIF gene, rs755622, and -794 CATT5-8, were genotyped using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and PCR followed by automated capillary electrophoresis, respectively. MIF mRNA levels in articular cartilage and subchondral bone were measured by quantitative polymerase chain reaction. Plasma concentrations of MIF, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) were measured by enzyme-linked immunosorbent assay. Results rs755622 and -794 CATT5-8 genotypes were not associated with MIF mRNA or protein levels or OA (all p ≥ 0.19). MIF mRNA level in cartilage was lower in OA patients than in controls (p = 0.028) and RA patients (p = 0.004), while the levels in bone were comparable between OA patients and controls (p = 0.165). MIF protein level in plasma was lower in OA patients than in controls (p = 3.01 × 10−10), while the levels of TNF-α, IL-6 and IL-1β in plasma were all significantly higher in OA patients than in controls (all p ≤ 0.0007). Multivariable logistic regression showed lower MIF and higher IL-1β protein levels in plasma were independently associated with OA (OR per SD increase = 0.10 and 8.08; 95% CI = 0.04–0.19 and 4.42–16.82, respectively), but TNF-α and IL-6 became non-significant. Conclusions Reduced MIF mRNA and protein expression in OA patients suggested MIF might have a protective role in OA and could serve as a biomarker to differentiate OA from other joint disorders.

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