scholarly journals Three-dimensional organization of nascent rod outer segment disk membranes

2015 ◽  
Vol 112 (48) ◽  
pp. 14870-14875 ◽  
Author(s):  
Stefanie Volland ◽  
Louise C. Hughes ◽  
Christina Kong ◽  
Barry L. Burgess ◽  
Kenneth A. Linberg ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Outer Segment ◽  
3D Visualization ◽  
Mammalian Species ◽  
Three Dimensional ◽  
Initiation Site ◽  
Rod Photoreceptor ◽  
Disk Formation ◽  
3D Data ◽  
Vertebrate Photoreceptor

The vertebrate photoreceptor cell contains an elaborate cilium that includes a stack of phototransductive membrane disks. The disk membranes are continually renewed, but how new disks are formed remains poorly understood. Here we used electron microscope tomography to obtain 3D visualization of the nascent disks of rod photoreceptors in three mammalian species, to gain insight into the process of disk morphogenesis. We observed that nascent disks are invariably continuous with the ciliary plasma membrane, although, owing to partial enclosure, they can appear to be internal in 2D profiles. Tomographic analyses of the basal-most region of the outer segment show changes in shape of the ciliary plasma membrane indicating an invagination, which is likely a first step in disk formation. The invagination flattens to create the proximal surface of an evaginating lamella, as well as membrane protrusions that extend between adjacent lamellae, thereby initiating a disk rim. Immediately distal to this initiation site, lamellae of increasing diameter are evident, indicating growth outward from the cilium. In agreement with a previous model, our data indicate that mature disks are formed once lamellae reach full diameter, and the growth of a rim encloses the space between adjacent surfaces of two lamellae. This study provides 3D data of nascent and mature rod photoreceptor disk membranes at unprecedented z-axis depth and resolution, and provides a basis for addressing fundamental questions, ranging from protein sorting in the photoreceptor cilium to photoreceptor electrophysiology.

scholarly journals 3-DIMENSIONAL GEOLOGICAL MAPPING AND MODELING ACTIVITIES AT THE GEOLOGICAL SURVEY OF NORWAY

2015 ◽  
Vol XL-2/W4 ◽  
pp. 11-16 ◽  
Author(s):  
A. Jarna ◽  
A. Bang-Kittilsen ◽  
C. Haase ◽  
I. H. C. Henderson ◽  
F. Høgaas ◽  
...  
Keyword(s):  
3D Visualization ◽  
Three Dimensional ◽  
Mineral Resources ◽  
Geological Mapping ◽  
Geological Survey ◽  
Marine Geology ◽  
Data Infrastructure ◽  
3 Dimensional ◽  
3D Data ◽  
Wide Range

Geology and all geological structures are three-dimensional in space. Geology can be easily shown as four-dimensional when time is considered. Therefore GIS, databases, and 3D visualization software are common tools used by geoscientists to view, analyse, create models, interpret and communicate geological data. The NGU (Geological Survey of Norway) is the national institution for the study of bedrock, mineral resources, surficial deposits and groundwater and marine geology. The interest in 3D mapping and modelling has been reflected by the increase of number of groups and researches dealing with 3D in geology within NGU. This paper highlights 3D geological modelling techniques and the usage of these tools in bedrock, geophysics, urban and groundwater studies at NGU, same as visualisation of 3D online. The examples show use of a wide range of data, methods, software and an increased focus on interpretation and communication of geology in 3D. The goal is to gradually expand the geospatial data infrastructure to include 3D data at the same level as 2D.

scholarly journals Immunocytochemical localization of opsin in the cell membrane of developing rat retinal photoreceptors.

1984 ◽  
Vol 98 (5) ◽  
pp. 1788-1795 ◽  
Author(s):  
I Nir ◽  
D Cohen ◽  
D S Papermaster
Keyword(s):  
Plasma Membrane ◽  
Outer Segment ◽  
Plasma Membranes ◽  
Photoreceptor Cells ◽  
Rod Photoreceptor ◽  
Rod Photoreceptors ◽  
Retinal Photoreceptors ◽  
Retinal Rod ◽  
Ros Formation ◽  
Developing Rat

Mature retinal rod photoreceptors sequester opsin in the disk and plasma membranes of the rod outer segment (ROS). Opsin is synthesized in the inner segment and is transferred to the outer segment along the connecting cilium that joins the two compartments. We have investigated early stages of retinal development during which the polarized distribution of opsin is established in the rod photoreceptor cell. Retinas were isolated from newborn rats, 3-21 d old, and incubated with affinity purified biotinyl-sheep anti-bovine opsin followed by avidin-ferritin. At early postnatal ages prior to the development of the ROS, opsin is labeled by antiopsin on the inner segment plasma membrane. At the fifth postnatal day, as ROS formation begins opsin was detected on the connecting cilium plasma membrane. However, the labeling density of the ciliary plasma membrane was not uniform: the proximal cilium was relatively unlabeled in comparison with the distal cilium and the ROS plasma membrane. In nearly mature rat retinas, opsin was no longer detected on the inner segment plasma membrane. A similar polarized distribution of opsin was also observed in adult human rod photoreceptor cells labeled with the same antibodies. These results suggest that some component(s) of the connecting cilium and its plasma membrane may participate in establishing and maintaining the polarized distribution of opsin.

scholarly journals Localization of peripherin/rds in the disk membranes of cone and rod photoreceptors: relationship to disk membrane morphogenesis and retinal degeneration.

1992 ◽  
Vol 116 (3) ◽  
pp. 659-667 ◽  
Author(s):  
K Arikawa ◽  
L L Molday ◽  
R S Molday ◽  
D S Williams
Keyword(s):  
Plasma Membrane ◽  
Retinal Degeneration ◽  
Outer Segment ◽  
Photoreceptor Cells ◽  
Growth Stages ◽  
Rod Outer Segments ◽  
Rod Photoreceptor ◽  
Outer Segments ◽  
Disk Membrane ◽  
Membrane Morphogenesis

The outer segments of vertebrate rod photoreceptor cells consist of an ordered stack of membrane disks, which, except for a few nascent disks at the base of the outer segment, is surrounded by a separate plasma membrane. Previous studies indicate that the protein, peripherin or peripherin/rds, is localized along the rim of mature disks of rod outer segments. A mutation in the gene for this protein has been reported to be responsible for retinal degeneration in the rds mouse. In the present study, we have shown by immunogold labeling of rat and ground squirrel retinas that peripherin/rds is present in the disk rims of cone outer segments as well as rod outer segments. Additionally, in the basal regions of rod and cone outer segments, where disk morphogenesis occurs, we have found that the distribution of peripherin/rds is restricted to a region that is adjacent to the cilium. Extension of its distribution from the cilium coincides with the formation of the disk rim. These results support the model of disk membrane morphogenesis that predicts rim formation to be a second stage of growth, after the first stage in which the ciliary plasma membrane evaginates to form open nascent disks. The results also indicate how the proteins of the outer segment plasma membrane and the disk membranes are sorted into their separate domains: different sets of proteins may be incorporated into membrane outgrowths during different growth stages of disk morphogenesis. Finally, the presence of peripherin/rds protein in both cone and rod outer segment disks, together with the phenotype of the rds mouse, which is characterized by the failure of both rod and cone outer segment formation, suggest that the same rds gene is expressed in both types of photoreceptor cells.

scholarly journals PRODUCING AND VISUALIZATING 3D BUILDING GEODATABASE AS A PART OF 3D CADASTRE PROJECT

2021 ◽  
Vol XLVI-4/W5-2021 ◽  
pp. 239-243
Author(s):  
A. Guntel ◽  
A. C. Aydinoglu
Keyword(s):  
3D Visualization ◽  
Three Dimensional ◽  
Information Modeling ◽  
Digital Data ◽  
Data Sets ◽  
3D Data ◽  
3D Cadastre ◽  
Real Estate Valuation ◽  
Geographic Data ◽  
Building Information

Abstract. Digital data production possibilities have developed with the emerging technologies, and it has become possible to use different data formats together. The usability of three-dimensional (3D) data on various application areas has increased with the multidimensional use of geographic data in established information systems, for 3D visualization, presentation, and analysis. Topography-related analyzes such as digital elevation models, digital terrain models, slope maps and visibility maps can be made from geographic data sets produced in 3D. In addition, the use of 3D data in Building Information Modeling (BIM) has added various innovations for geographic data analysis. In this study, a geographic database was established by taking the vector data produced in the 3D Cadastre project that was carried out by the General Directorate of Land Registry and Cadastre as an example. Data obtained from photogrammetry and architectural projects were used in accordance with the OGC CityGML standard. After creating 3D building database in GIS environment, as result of various visualization and analysis techniques, the contributions of this project to BIM were revealed for various applications such as real estate valuation, disaster management, renewable energy, 3D city models, and smart city projects.

scholarly journals Three-dimensional architecture of murine rod outer segments determined by cryoelectron tomography

2007 ◽  
Vol 177 (5) ◽  
pp. 917-925 ◽  
Author(s):  
Stephan Nickell ◽  
Paul S.-H. Park ◽  
Wolfgang Baumeister ◽  
Krzysztof Palczewski
Keyword(s):  
Plasma Membrane ◽  
Mammalian Species ◽  
Three Dimensional ◽  
Photoreceptor Cells ◽  
Light Signal ◽  
Proper Distance ◽  
Theoretical Approaches ◽  
Internal Structures ◽  
Membrane Components ◽  
Important Structure

The rod outer segment (ROS) of photoreceptor cells houses all components necessary for phototransduction, a set of biochemical reactions that amplify and propagate a light signal. Theoretical approaches to quantify this process require precise information about the physical boundaries of the ROS. Dimensions of internal structures within the ROS of mammalian species have yet to be determined with the precision required for quantitative considerations. Cryoelectron tomography was utilized to obtain reliable three-dimensional morphological information about this important structure from murine retina. Vitrification of samples permitted imaging of the ROS in a minimally perturbed manner and the preservation of substructures. Tomograms revealed the characteristic highly organized arrangement of disc membranes stacked on top of one another with a surrounding plasma membrane. Distances among the various membrane components of the ROS were measured to define the space available for phototransduction to occur. Reconstruction of segments of the ROS from single-axis tilt series images provided a glimpse into the three-dimensional architecture of this highly differentiated neuron. The reconstructions revealed spacers that likely maintain the proper distance between adjacent discs and between discs and the plasma membrane. Spacers were found distributed throughout the discs, including regions that are distant from the rim region of discs.

scholarly journals Biosynthesis and vectorial transport of opsin on vesicles in retinal rod photoreceptors.

1986 ◽  
Vol 34 (1) ◽  
pp. 5-16 ◽  
Author(s):  
D S Papermaster ◽  
B G Schneider ◽  
D DeFoe ◽  
J C Besharse
Keyword(s):  
Plasma Membrane ◽  
Outer Segment ◽  
Photoreceptor Cells ◽  
Disk Surface ◽  
Light Sensitivity ◽  
Apical Plasma Membrane ◽  
Rod Photoreceptor ◽  
Electron Microscopic ◽  
Retinal Rod ◽  
Membrane Bound

Retinal rod photoreceptor cells absorb light at one end and establish synaptic contacts on the other. Light sensitivity is conferred by a set of membrane and cytosol proteins that are gathered at one end of the cell to form a specialized organelle, the rod outer segment (ROS). The ROS is composed of rhodopsin-laden, flattened disk-shaped membranes enveloped by the cell's plasma membrane. Rhodopsin is synthesized on elements of the rough endoplasmic reticulum and Golgi apparatus near the nucleus in the inner segment. From this synthetic site, the membrane-bound apoprotein, opsin, is released from the Golgi in the membranes of small vesicles. These vesicles are transported through the cytoplasm of the inner segment until they reach its apical plasma membrane. At that site, opsin-laden vesicles appear to fuse near the base of the connecting cilium that joins the inner and outer segments. This fusion inserts opsin into the plasma membrane of the photoreceptor. Opsin becomes incorporated into the disk membrane by a process of membrane expansion and fusion to form the flattened disks of the outer segment. Within the disks, opsin is highly mobile, and rapidly rotates and traverses the disk surface. Despite its mobility in the outer segment, quantitative electron microscopic, immunocytochemical, and autoradiographic studies of opsin distribution demonstrate that little opsin is detectable in the inner segment plasma membrane, although its bilayer is in continuity with the plasma membrane of the outer segment. The photoreceptor successfully establishes the polarized distribution of its membrane proteins by restricting the redistribution of opsin after vectorially transporting it to one end of the cell on post-Golgi vesicles.

scholarly journals Distribution of guanylate cyclase within photoreceptor outer segments

1996 ◽  
Vol 109 (7) ◽  
pp. 1803-1812
Author(s):  
M.A. Hallett ◽  
J.L. Delaat ◽  
K. Arikawa ◽  
C.L. Schlamp ◽  
F. Kong ◽  
...  
Keyword(s):  
Guanylate Cyclase ◽  
Actin Filaments ◽  
Outer Segment ◽  
Essential Role ◽  
Photoreceptor Cells ◽  
Rod Photoreceptor ◽  
Light Activation ◽  
Photoreceptor Outer Segment ◽  
Photoreceptor Outer Segments ◽  
Vertebrate Photoreceptor

Guanylate cyclases play an essential role in the recovery of vertebrate photoreceptor cells after light activation. Here, we have investigated how one such guanylate cyclase, RetGC-1, is distributed within light- and dark-adapted rod photoreceptor cells. Guanylate cyclase activity partitioned with the photoreceptor outer segment (OS) cytoskeleton in a light-sensitive manner. RetGC-1 was found to bind actin filaments in actin blot overlays, suggesting a mechanism for its association with the OS cytoskeleton. In retinal sections, this enzyme was immunodetected only in the OSs, where it appeared to be distributed throughout the disk membranes.

scholarly journals Freeze-fracture studies of photoreceptor membranes: new observations bearing upon the distribution of cholesterol.

1983 ◽  
Vol 97 (3) ◽  
pp. 749-755 ◽  
Author(s):  
L D Andrews ◽  
A I Cohen
Keyword(s):  
New York ◽  
Plasma Membrane ◽  
Outer Segment ◽  
Plasma Membranes ◽  
Prolonged Exposure ◽  
Freeze Fracture ◽  
Cholesterol Content ◽  
Apical Plasma Membrane ◽  
Frog Neuromuscular Junction ◽  
Vertebrate Photoreceptor

We performed electron microscopy of replicas from freeze-fractured retinas exposed during or after fixation to the cholesterol-binding antibiotic, filipin. We observed characteristic filipin-induced perturbations throughout the disk and plasma membranes of retinal rod outer segments of various species. It is evident that a prolonged exposure to filipin in fixative enhances rather than reduces presumptive cholesterol detection in the vertebrate photoreceptor cell. In agreement with the pattern seen in our previous study (Andrews, L.D., and A. I. Cohen, 1979, J. Cell Biol., 81:215-228), filipin-binding in membranes exhibiting particle-free patches seemed largely confined to these patches. Favorably fractured photoreceptors exhibited marked filipin-binding in apical inner segment plasma membrane topologically confluent with and proximate to the outer segment plasma membrane, which was comparatively free of filipin binding. A possible boundary between these differing membrane domains was suggested in a number of replicas exhibiting lower filipin binding to the apical plasma membrane of the inner segment in the area surrounding the cilium. This area contains a structure (Andrews, L. D., 1982, Freeze-fracture studies of vertebrate photoreceptors, In Structure of the Eye, J. G. Hollyfield and E. Acosta Vidrio, editors, Elsevier/North-Holland, New York, 11-23) that resembles the active zones of the nerve terminals for the frog neuromuscular junction. These observations lead us to hypothesize that these structures may function to direct vesicle fusion to occur near them, in a domain of membrane more closely resembling outer than inner segment plasma membrane. The above evidence supports the views that (a) all disk membranes contain cholesterol, but the particle-free patches present in some disks trap cholesterol from contiguous particulate membrane regions; (b) contiguous inner and outer segment membranes may greatly differ in cholesterol content; and (c) the suggested higher cholesterol in the inner segment than in the outer segment plasma membrane may help direct newly inserted photopigment molecules to the outer segment.

scholarly journals Developing a Tile-Based Rendering Method to Improve Rendering Speed of 3D Geospatial Data with HTML5 and WebGL

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Seokchan Kang ◽  
Jiyeong Lee
Keyword(s):  
Spatial Data ◽  
Bin Packing ◽  
3D Visualization ◽  
Three Dimensional ◽  
Real Data ◽  
Web Based ◽  
3D Data ◽  
City Modeling ◽  
Internet Explorer ◽  
Modeling Data

A dedicated plug-in has been installed to visualize three-dimensional (3D) city modeling spatial data in web-based applications. However, plug-in methods are gradually becoming obsolete, owing to their limited performance with respect to installation errors, unsupported cross-browsers, and security vulnerability. Particularly, in 2015, the NPAPI service was terminated in most existing web browsers except Internet Explorer. To overcome these problems, the HTML5/WebGL (next-generation web standard, confirmed in October 2014) technology emerged. In particular, WebGL is able to display 3D spatial data without plug-ins in browsers. In this study, we attempted to identify the requirements and limitations of displaying 3D city modeling spatial data using HTML5/WebGL, and we propose alternative ways based on the bin-packing algorithm that aggregates individual 3D city modeling data including buildings in tile units. The proposed method reduces the operational complexity and the number and volume of transmissions required for rendering processing to improve the speed of 3D data rendering. The proposed method was validated on real data for evaluating its effectiveness in 3D visualization of city modeling data in web-based applications.

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