scholarly journals Identification of phosphoproteins correlated with proliferation and cell cycle arrest in Saccharomyces cerevisiae: positive and negative regulation by cAMP-dependent protein kinase.

1986 ◽  
Vol 83 (16) ◽  
pp. 5973-5977 ◽  
Author(s):  
M. L. Tripp ◽  
R. Pinon ◽  
J. Meisenhelder ◽  
T. Hunter
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Protein Kinase ◽  
Cell Cycle Arrest ◽  
Negative Regulation ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Cycle Arrest ◽  
Dependent Protein

scholarly journals The Saccharomyces cerevisiae YAK1 gene encodes a protein kinase that is induced by arrest early in the cell cycle.

1991 ◽  
Vol 11 (8) ◽  
pp. 4045-4052 ◽  
Author(s):  
S Garrett ◽  
M M Menold ◽  
J R Broach
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Kinase Activity ◽  
Specific Activity ◽  
S Phase ◽  
Protein Kinase Activity ◽  
Dependent Protein Kinase ◽  
Protein Levels ◽  
Cycle Arrest ◽  
Dependent Protein

Null mutations in the gene YAK1, which encodes a protein with sequence homology to known protein kinases, suppress the cell cycle arrest phenotype of mutants lacking the cyclic AMP-dependent protein kinase (A kinase). That is, loss of the YAK1 protein specifically compensates for loss of the A kinase. Here, we show that the protein encoded by YAK1 has protein kinase activity. Yak1 kinase activity is low during exponential growth but is induced at least 50-fold by arrest of cells prior to the completion of S phase. Induction is not observed by arrest at stages later in the cell cycle. Depending on the arrest regimen, induction can occur either by an increase in Yak1 protein levels or by an increase in Yak1 specific activity. Finally, an increase in Yak1 protein levels causes growth arrest of cells with attenuated A kinase activity. These results suggest that Yak1 acts in a pathway parallel to that of the A kinase to negatively regulate cell proliferation.

The Saccharomyces cerevisiae SRK1 gene, a suppressor of bcy1 and ins1, may be involved in protein phosphatase function

1991 ◽  
Vol 11 (6) ◽  
pp. 3369-3373
Author(s):  
R B Wilson ◽  
A A Brenner ◽  
T B White ◽  
M J Engler ◽  
J P Gaughran ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Protein Phosphatase ◽  
Shuttle Vector ◽  
Temperature Sensitive ◽  
Protein Kinase Activity ◽  
Dependent Protein Kinase ◽  
Cycle Arrest ◽  
Dependent Protein

The Saccharomyces cerevisiae SRK1 gene, when expressed on a low-copy shuttle vector, partially suppresses the phenotype associated with elevated levels of cyclic AMP-dependent protein kinase activity and suppresses the temperature-sensitive cell cycle arrest of the ins1 mutant. SRK1 is located on chromosome IV, 3 centimorgans from gcn2. A mutant carrying a deletion mutation in srk1 is viable. SRK1 encodes a 140-kDa protein with homology to the dis3+ protein from Schizosaccharomyces pombe. The ability of SRK1 to alleviate partially the defects caused by high levels of cyclic AMP-dependent protein kinase and the similarity of its encoded protein to dis3+ suggest that SRK1 may have a role in protein phosphatase function.

scholarly journals Ras/cAMP-dependent Protein Kinase (PKA) Regulates Multiple Aspects of Cellular Events by Phosphorylating the Whi3 Cell Cycle Regulator in Budding Yeast

2013 ◽  
Vol 288 (15) ◽  
pp. 10558-10566 ◽  
Author(s):  
Masaki Mizunuma ◽  
Ryohei Tsubakiyama ◽  
Takafumi Ogawa ◽  
Atsunori Shitamukai ◽  
Yoshifumi Kobayashi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Budding Yeast ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Cellular Events ◽  
Cell Cycle Regulator ◽  
Dependent Protein
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