scholarly journals Developmental and tissue-specific regulation of the Q10 class I gene by DNA methylation.

1986 ◽  
Vol 83 (20) ◽  
pp. 7598-7602 ◽  
Author(s):  
K. Tanaka ◽  
Y. Barra ◽  
K. J. Isselbacher ◽  
G. Khoury ◽  
G. Jay
Keyword(s):  
Dna Methylation ◽  
Class I ◽  
Tissue Specific ◽  
Specific Regulation ◽  
I Gene

Stable transfer and restricted expression of a cloned class I gene encoding a secreted transplantation-like antigen

1985 ◽  
Vol 5 (6) ◽  
pp. 1295-1300
Author(s):  
Y Barra ◽  
K Tanaka ◽  
K J Isselbacher ◽  
G Khoury ◽  
G Jay
Keyword(s):  
Molecular Mechanisms ◽  
Cell Types ◽  
Class I ◽  
Regulatory Sequences ◽  
Transcriptional Enhancer ◽  
Gene Encoding ◽  
Specific Expression ◽  
Tissue Specific ◽  
Functional Studies ◽  
I Gene

The identification of a unique major histocompatibility complex class I gene, designated Q10, which encodes a secreted rather than a cell surface antigen has led to questions regarding its potential role in regulating immunological functions. Since the Q10 gene is specifically activated only in the liver, we sought to define the molecular mechanisms which control its expression in a tissue-specific fashion. Results obtained by transfection of the cloned Q10 gene, either in the absence or presence of a heterologous transcriptional enhancer, into a variety of cell types of different tissue derivations are consistent with the Q10 gene being regulated at two levels. The first is by a cis-dependent mechanism which appears to involve site-specific DNA methylation. The second is by a trans-acting mechanism which would include the possibility of an enhancer binding factor. The ability to efficiently express the Q10 gene in certain transfected cell lines offers an opportunity to obtain this secreted class I antigen in quantities sufficient for functional studies; this should also make it possible to define regulatory sequences which may be responsible for the tissue-specific expression of Q10.

scholarly journals Human leukocyte antigen F (HLA-F). An expressed HLA gene composed of a class I coding sequence linked to a novel transcribed repetitive element.

1990 ◽  
Vol 171 (1) ◽  
pp. 1-18 ◽  
Author(s):  
D E Geraghty ◽  
X H Wei ◽  
H T Orr ◽  
B H Koller
Keyword(s):  
Dna Sequence ◽  
Hla Class I ◽  
Class I ◽  
Northern Analysis ◽  
Protein Sequence Analysis ◽  
Specific Expression ◽  
Rnase Protection ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
I Gene

We describe here the isolation and sequencing of a previously uncharacterized HLA class I gene. This gene, HLA-5.4, is the third non-HLA-A,B,C gene characterized whose sequence shows it encodes an intact class I protein. RNase protection assays with a probe specific for this gene demonstrated its expression in B lymphoblastoid cell lines, in resting T cells, and skin cells, while no mRNA could be detected in the T cell line Molt 4. Consistent with a pattern of expression different from that of other class I genes, DNA sequence comparisons identified potential regulator motifs unique to HLA-5.4 and possibly essential for tissue-specific expression. Protein sequence analysis of human and murine class I antigens has identified 10 highly conserved residues believed to be involved in antigen binding. Five of these are altered in HLA-5.4, and of these, three are nonconservative. In addition, examination of the HLA-5.4 DNA sequence predicts that the cytoplasmic segment of this protein is shorter than that of the classical transplantation antigens. The 3' untranslated region of the HLA-5.4 gene contains one member of a previously undescribed multigene family consisting of at least 30 members. Northern analysis showed that several of these sequences were transcribed, and the most ubiquitous transcript, a 600-nucleotide polyadenylated mRNA, was found in all tissues and cells examined. This sequence is conserved in the mouse genome, where a similar number of copies were found, and one of these sequences was also transcribed, yielding a 600-nucleotide mRNA. The characterization of this unique HLA class I gene and the demonstration of its tissue-specific expression have prompted us to propose that HLA-5.4 be designated HLA-F.

scholarly journals Stable transfer and restricted expression of a cloned class I gene encoding a secreted transplantation-like antigen.

1985 ◽  
Vol 5 (6) ◽  
pp. 1295-1300 ◽  
Author(s):  
Y Barra ◽  
K Tanaka ◽  
K J Isselbacher ◽  
G Khoury ◽  
G Jay
Keyword(s):  
Molecular Mechanisms ◽  
Cell Types ◽  
Class I ◽  
Regulatory Sequences ◽  
Transcriptional Enhancer ◽  
Gene Encoding ◽  
Specific Expression ◽  
Tissue Specific ◽  
Functional Studies ◽  
I Gene

The identification of a unique major histocompatibility complex class I gene, designated Q10, which encodes a secreted rather than a cell surface antigen has led to questions regarding its potential role in regulating immunological functions. Since the Q10 gene is specifically activated only in the liver, we sought to define the molecular mechanisms which control its expression in a tissue-specific fashion. Results obtained by transfection of the cloned Q10 gene, either in the absence or presence of a heterologous transcriptional enhancer, into a variety of cell types of different tissue derivations are consistent with the Q10 gene being regulated at two levels. The first is by a cis-dependent mechanism which appears to involve site-specific DNA methylation. The second is by a trans-acting mechanism which would include the possibility of an enhancer binding factor. The ability to efficiently express the Q10 gene in certain transfected cell lines offers an opportunity to obtain this secreted class I antigen in quantities sufficient for functional studies; this should also make it possible to define regulatory sequences which may be responsible for the tissue-specific expression of Q10.

Tissue-specific expression of the mouse Q10 H-2 class-I gene during embryogenesis

Gene ◽  
1987 ◽  
Vol 61 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Brigitte David-Watine ◽  
Catherine Transy ◽  
Gabriel Gachelin ◽  
Philippe Kourilsky
Keyword(s):  
Class I ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
I Gene

Liver-specific expression of a Qa-encoded class I gene is associated with DNA hypomethylation

1986 ◽  
Vol 6 (1) ◽  
pp. 315-317
Author(s):  
C G Miyada ◽  
R B Wallace
Keyword(s):  
Dna Methylation ◽  
Major Histocompatibility Complex ◽  
Class I ◽  
Transplantation Antigen ◽  
Chromosomal Dna ◽  
Dna Hypomethylation ◽  
Specific Expression ◽  
Histocompatibility Complex ◽  
Dna Restriction ◽  
I Gene

DNA methylation of two murine major histocompatibility complex (H-2) class I genes was examined in hybridizations to MspI and HpaII chromosomal DNA restriction digests. Q10, which exhibits liver-specific expression, and H-2Kb, a transplantation antigen gene, were examined in liver, spleen, thymus, and cell-line DNAs. Unmethylated Q10 gene sequences were detected only in the liver, whereas the H-2Kb gene was unmethylated in all tissues examined.

scholarly journals DNA Methylation Regulates Placental Lactogen I Gene Expression

Endocrinology ◽  
2001 ◽  
Vol 142 (8) ◽  
pp. 3389-3396 ◽  
Author(s):  
Jae-Hyeon Cho ◽  
Hiromichi Kimura ◽  
Tatsuya Minami ◽  
Jun Ohgane ◽  
Naka Hattori ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Promoter Region ◽  
Trichostatin A ◽  
Methylation Status ◽  
Placental Lactogen ◽  
Specific Gene ◽  
Tissue Specific ◽  
Reporter Activity ◽  
I Gene

Abstract Expression of rat placental lactogen I is specific to the placenta and never expressed in other tissues. To obtain insight into the mechanism of tissue-specific gene expression, we investigated the methylation status in 3.4 kb of the 5′-flanking region of the rat placental lactogen I gene. We found that the distal promoter region of the rat placental lactogen I gene had more potent promoter activity than that of the proximal area alone, which contains several possible cis-elements. Although there are only 17 CpGs in the promoter region, in vitro methylation of the reporter constructs caused severe suppression of reporter activity, and CpG sites in the placenta were more hypomethylated than other tissues. Coexpression of methyl-CpG-binding protein with reporter constructs elicited further suppression of the reporter activity, whereas treatment with trichostatin A, an inhibitor of histone deacetylase, reversed the suppression caused by methylation. Furthermore, treatment of rat placental lactogen I nonexpressing BRL cells with 5-aza-2′-deoxycytidine, an inhibitor of DNA methylation, or trichostatin A resulted in the de novo expression of rat placental lactogen I. These results provide evidence that change in DNA methylation is the fundamental mechanism regulating the tissue-specific expression of the rat placental lactogen I gene.

Locus specific regulation of HLA class I gene expression

1996 ◽  
Vol 47 (1-2) ◽  
pp. 11 ◽  
Author(s):  
S.J.P. Gobin ◽  
V. Keijsers ◽  
A.M. Woltman ◽  
A. Peijnenburg ◽  
L. Wilson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hla Class I ◽  
Class I ◽  
Specific Regulation ◽  
I Gene

scholarly journals Liver-specific expression of a Qa-encoded class I gene is associated with DNA hypomethylation.

1986 ◽  
Vol 6 (1) ◽  
pp. 315-317 ◽  
Author(s):  
C G Miyada ◽  
R B Wallace
Keyword(s):  
Dna Methylation ◽  
Major Histocompatibility Complex ◽  
Class I ◽  
Transplantation Antigen ◽  
Chromosomal Dna ◽  
Dna Hypomethylation ◽  
Specific Expression ◽  
Histocompatibility Complex ◽  
Dna Restriction ◽  
I Gene

DNA methylation of two murine major histocompatibility complex (H-2) class I genes was examined in hybridizations to MspI and HpaII chromosomal DNA restriction digests. Q10, which exhibits liver-specific expression, and H-2Kb, a transplantation antigen gene, were examined in liver, spleen, thymus, and cell-line DNAs. Unmethylated Q10 gene sequences were detected only in the liver, whereas the H-2Kb gene was unmethylated in all tissues examined.

Sign in / Sign up

Export Citation Format

Share Document