scholarly journals Characterization and cDNA cloning of the pheromone-binding protein from the tobacco hornworm, Manduca sexta: a tissue-specific developmentally regulated protein.

1988 ◽  
Vol 85 (24) ◽  
pp. 9851-9855 ◽  
Author(s):  
T. K. Gyorgyi ◽  
A. J. Roby-Shemkovitz ◽  
M. R. Lerner
Keyword(s):  
Manduca Sexta ◽  
Cdna Cloning ◽  
Binding Protein ◽  
Tobacco Hornworm ◽  
Developmentally Regulated ◽  
Tissue Specific ◽  
Pheromone Binding Protein

scholarly journals NBP-45, a Novel Nucleosomal Binding Protein with a Tissue-specific and Developmentally Regulated Expression

2000 ◽  
Vol 275 (9) ◽  
pp. 6368-6374 ◽  
Author(s):  
Hitoshi Shirakawa ◽  
David Landsman ◽  
Yuri V. Postnikov ◽  
Michael Bustin
Keyword(s):  
Binding Protein ◽  
Developmentally Regulated ◽  
Tissue Specific ◽  
Regulated Expression

Comparative properties of tobacco hornworm, Manduca sexta, carbonic anhydrases

1981 ◽  
Vol 241 (1) ◽  
pp. R92-R99
Author(s):  
A. M. Jungreis ◽  
N. D. Barron ◽  
J. W. Johnston
Keyword(s):  
Alkali Metal ◽  
Manduca Sexta ◽  
Fat Body ◽  
Metal Cations ◽  
Tobacco Hornworm ◽  
Carbonic Anhydrases ◽  
Alkali Metal Cations ◽  
Halide Anion ◽  
Tissue Specific ◽  
Q10 Values

Comparative properties of midgut, fat body and integumentary carbonic anhydrases (CAs) were measured as functions of CA-catalyzed CO2 hydration or p-nitrophenylacetate (pNPA) hydrolysis in feeding larvae and pharate pupae. Alkali metal cations fully reverse the halide anion inhibition of CA catalyzed CO2 hydration during the larval but not the pharate pupal stages in development. Concentrations of acetazolamide required to inhibit 50% of tissue-specific CA-catalyzed CO2 hydration (I50) were approximately 10(-8) M. The activity profiles between pH 6.0 and 9.0 had well-defined optima around pH 8.0 with activity declining dramatically above the optima. When CA-catalyzed pNPA hydrolysis was measured between 4 and 37 degrees C, insect enzymes failed to exhibit positive Q10 values between 25 and 37 degrees C. The affinities of the hornworm CAs toward pNPA were determined with stage- and tissue-specific Km ranging from 0.42 to 8.0 X 10(-3) M. It is concluded that tissue and stage-specific CAs exist in M. sexta, whose properties appear to differ markedly with those previously reported for mammalian enzymes.

scholarly journals Cloning and Expression of a Queen Pheromone-Binding Protein in the Honeybee: an Olfactory-Specific, Developmentally Regulated Protein

1999 ◽  
Vol 19 (17) ◽  
pp. 7468-7475 ◽  
Author(s):  
Emmanuelle Danty ◽  
Loı̈c Briand ◽  
Christine Michard-Vanhée ◽  
Valérie Perez ◽  
Gérard Arnold ◽  
...  
Keyword(s):  
Binding Protein ◽  
Queen Pheromone ◽  
Cloning And Expression ◽  
Developmentally Regulated ◽  
Pheromone Binding Protein
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