scholarly journals The retinoblastoma-susceptibility gene product binds directly to the human TATA-binding protein-associated factor TAFII250.

1995 ◽  
Vol 92 (8) ◽  
pp. 3115-3119 ◽  
Author(s):  
Z. Shao ◽  
S. Ruppert ◽  
P. D. Robbins
Author(s):  
Michael A. Clegg ◽  
Natalie H. Theodoulou ◽  
Paul Bamborough ◽  
Chun-wa Chung ◽  
Peter D. Craggs ◽  
...  

2018 ◽  
Vol 1 (5) ◽  
pp. e201800082 ◽  
Author(s):  
Marte Molenaars ◽  
Georges E Janssens ◽  
Toon Santermans ◽  
Marco Lezzerini ◽  
Rob Jelier ◽  
...  

Mutations in the clk-1 gene impair mitochondrial ubiquinone biosynthesis and extend the lifespan in Caenorhabditis elegans. We demonstrate here that this life extension is linked to the repression of cytoplasmic mRNA translation, independent of the alleged nuclear form of CLK-1. Clk-1 mutations inhibit polyribosome formation similarly to daf-2 mutations that dampen insulin signaling. Comparisons of total versus polysomal RNAs in clk-1(qm30) mutants reveal a reduction in the translational efficiencies of mRNAs coding for elements of the translation machinery and an increase in those coding for the oxidative phosphorylation and autophagy pathways. Knocking down the transcription initiation factor TATA-binding protein-associated factor 4, a protein that becomes sequestered in the cytoplasm during early embryogenesis to induce transcriptional silencing, ameliorates the clk-1 inhibition of polyribosome formation. These results underscore a prominent role for the repression of cytoplasmic protein synthesis in eukaryotic lifespan extension and suggest that mutations impairing mitochondrial function are able to exploit this repression similarly to reductions of insulin signaling. Moreover, this report reveals an unexpected role for TATA-binding protein-associated factor 4 as a repressor of polyribosome formation when ubiquinone biosynthesis is compromised.


1998 ◽  
Vol 273 (23) ◽  
pp. 14293-14300 ◽  
Author(s):  
Josef Ozer ◽  
Katherine Mitsouras ◽  
Dennis Zerby ◽  
Michael Carey ◽  
Paul M. Lieberman

2000 ◽  
Vol 74 (13) ◽  
pp. 6096-6104 ◽  
Author(s):  
Yasuko Mori ◽  
Panadda Dhepakson ◽  
Takuya Shimamoto ◽  
Keiji Ueda ◽  
Yasuyuki Gomi ◽  
...  

ABSTRACT We have characterized the human herpesvirus 6B (HHV-6B)rep gene, which is a homologue of the adeno-associated virus type 2 rep and is unique in the herpesvirus family. Three transcripts, 9.0, 5.0, and 2.7 kb (the major transcript), were detected by Northern blotting using an HHV-6B rep probe under late conditions. We investigated the expression kinetics of therep gene using cycloheximide (CHX) and phosphonoformic acid (PFA), which are inhibitors of protein synthesis and viral DNA synthesis, respectively. The 5.2-kb transcript was mainly detected in the absence of protein biosynthesis upon infection, and none of the 9.0-, 5.0-, and 2.7-kb transcripts detected under the late conditions were detected in the presence of CHX and PFA. Sequences obtained from a cDNA library showed that the 5.0- and 2.7-kb transcripts were spliced from two and three exons, respectively, and the 2.7-kb transcript was more abundant. Immunohistochemistry using an antibody raised against the HHV-6 rep gene product (REP) revealed that REP was mainly present in the nucleus of MT-4 cells within 24 h after infection with HHV-6B. Using pull-down assays, coimmunoprecipitation, and a mammalian two hybrid system, we showed that HHV-6 REP binds to a transcription factor, human TATA-binding protein, through its N-terminal region.


2007 ◽  
Vol 27 (8) ◽  
pp. 2886-2896 ◽  
Author(s):  
Arindam Dasgupta ◽  
Rebekka O. Sprouse ◽  
Sarah French ◽  
Pavel Aprikian ◽  
Robert Hontz ◽  
...  

ABSTRACT Mot1 is an essential, conserved, TATA-binding protein (TBP)-associated factor in Saccharomyces cerevisiae with well-established roles in the global control of RNA polymerase II (Pol II) transcription. Previous results have suggested that Mot1 functions exclusively in Pol II transcription, but here we report a novel role for Mot1 in regulating transcription by RNA polymerase I (Pol I). In vivo, Mot1 is associated with the ribosomal DNA, and loss of Mot1 results in decreased rRNA synthesis. Consistent with a direct role for Mot1 in Pol I transcription, Mot1 also associates with the Pol I promoter in vitro in a reaction that depends on components of the Pol I general transcription machinery. Remarkably, in addition to Mot1's role in initiation, rRNA processing is delayed in mot1 cells. Taken together, these results support a model in which Mot1 affects the rate and efficiency of rRNA synthesis by both direct and indirect mechanisms, with resulting effects on transcription activation and the coupling of rRNA synthesis to processing.


2000 ◽  
Vol 345 (3) ◽  
pp. 521 ◽  
Author(s):  
Jan A. VAN DER KNAAP ◽  
Vincent VAN DEN BOOM ◽  
Jeroen KUIPERS ◽  
Michiel J.T. VAN EIJK ◽  
Peter C. VAN DER VLIET ◽  
...  

2007 ◽  
Vol 282 (30) ◽  
pp. 22228-22238 ◽  
Author(s):  
Tapas K. Mal ◽  
Shinya Takahata ◽  
Sewon Ki ◽  
Le Zheng ◽  
Tetsuro Kokubo ◽  
...  

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