scholarly journals Transgenic male-sterile plant induced by an unedited atp9 gene is restored to fertility by inhibiting its expression with antisense RNA.

1996 ◽  
Vol 93 (20) ◽  
pp. 11259-11263 ◽  
Author(s):  
E. Zabaleta ◽  
A. Mouras ◽  
M. Hernould ◽  
Suharsono ◽  
A. Araya
Keyword(s):  
Antisense Rna ◽  
Sterile Plant ◽  
Male Sterile ◽  
Male Sterile Plant ◽  
Transgenic Male

scholarly journals Seed-set evaluation of four male-sterile, female-fertile soybean lines using alfalfa leafcutting bees and honey bees as pollinators

2008 ◽  
Vol 146 (4) ◽  
pp. 461-469 ◽  
Author(s):  
E. ORTIZ-PEREZ ◽  
R. M. A. MIAN ◽  
R. L. COOPER ◽  
T. MENDIOLA ◽  
J. TEW ◽  
...  
Keyword(s):  
Honey Bees ◽  
Seed Set ◽  
Soybean Seed ◽  
Sterile Plant ◽  
Male Sterile ◽  
Male Sterile Plant ◽  
Local Conditions ◽  
Sterile Female ◽  
Cross Pollination ◽  
Pollinator Species

SUMMARYMale-sterile, female-fertile plants were used to produce hybrid soybean seed. Manual cross-pollination using male-sterile plants to produce large quantities of hybrid seed is difficult and time-consuming because of the low success rate in cross-pollination. Insect pollinators may be suitable vectors to transfer pollen, but the most suitable vector for pollen transfer from the male parent to the female parent has not been identified for soybean. The objective of the present study was to evaluate seed-set on four male-sterile, female-fertile soybean lines by using alfalfa leafcutting bees (Megachile rotundata (F.)) and honey bees (Apis mellifera (L.)) as pollinators. Seed-set was evaluated in summers 2003 and 2005 near Ames, Iowa, USA and in summers 2003, 2004, and 2005 near Wooster, Ohio, USA. Neither the effect of pollinator species nor the interaction effect of pollinator species×location was significant for any year. Honey bees performed similarly to alfalfa leafcutting bees at both locations. The results indicated significant differences for seed-set among male-sterile lines, suggesting preferential pollination. Male-sterile lines, ms1 (Urbana) and ms2 (Ames 2), had higher cross-pollinated seed-set compared to ms6 (Ames 1), and ms6 (Corsoy 79). At the Ames location, ms1ms1 (Urbana) plants had the highest seed-set (50·16 seeds per male-sterile plant in 2005). At the Wooster location, ms1ms1 (Urbana) plants also had the highest seed-set (92·04 seeds per male-sterile plant) in 2005. Costs and local conditions need to be addressed to support the choice of either pollinator species as a pollination vector to produce hybrid soybean seed.

scholarly journals Slow Development Restores the Fertility of Photoperiod-Sensitive Male-Sterile Plant Lines

2020 ◽  
Vol 184 (2) ◽  
pp. 923-932
Author(s):  
Cheng Zhang ◽  
Te Xu ◽  
Meng-Yi Ren ◽  
Jun Zhu ◽  
Qiang-Sheng Shi ◽  
...  
Keyword(s):  
Sterile Plant ◽  
Male Sterile ◽  
Male Sterile Plant ◽  
Slow Development ◽  
Photoperiod Sensitive

Slowing development restores the fertility of thermo-sensitive male-sterile plant lines

Nature Plants ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 360-367 ◽  
Author(s):  
Jun Zhu ◽  
Yue Lou ◽  
Qiang-Sheng Shi ◽  
Sen Zhang ◽  
Wen-Tao Zhou ◽  
...  
Keyword(s):  
Sterile Plant ◽  
Male Sterile ◽  
Male Sterile Plant

DIGENIC INHERITANCE OF MALE STERILITY IN PLANTAGO LANCEOLATA

1969 ◽  
Vol 11 (3) ◽  
pp. 739-744 ◽  
Author(s):  
M. D. Ross
Keyword(s):  
Male Sterility ◽  
Plantago Lanceolata ◽  
Sterile Plant ◽  
Male Sterile ◽  
Male Sterile Plant ◽  
Digenic Inheritance ◽  
Dominant Genes

Gynodioecy is an outbreeding mechanism where both male-sterile (i.e. female) and hermaphrodite plants are found. Plantago lanceolata is gynodioecious, and ratios of 1:1, 3:1, 7:1 and 15:1 obtained in the descendants of a male-sterile plant indicate that there are two duplicate dominant genes for hermaphroditism, and that the double recessive, ms1ms1ms2ms2, is male sterile. Descendants of other male-sterile plants produced different results suggesting the presence of some additional factor affecting the inheritance of male sterility.

Male sterility in Indian mustard (Brassica juncea (L.) Coss). IV. Genetics of MS-4

1985 ◽  
Vol 27 (5) ◽  
pp. 487-490 ◽  
Author(s):  
Surinder S. Banga ◽  
K. S. Labana
Keyword(s):  
Male Sterility ◽  
Brassica Juncea ◽  
Indian Mustard ◽  
Field Trials ◽  
Genetic Constitution ◽  
Male Sterile ◽  
Fertility Restorer ◽  
Male Sterile Plant ◽  
Restorer Genes ◽  
Fertility Restorer Genes

Male sterile plants of Indian mustard (Brassica juncea (L.) Coss.) were observed in the F2 generation of the cross RLM-198 × EJ-33. The genetic analysis revealed that male sterility occurred when the cytoplasm of RLM-198 interacted with recessive nuclear genes of EJ-33. The genetic constitution of RLM-198 was postulated to be (S) RF RF, EJ-33 as (F) rf rf, and the male sterile plants as (S) rf rf. Varieties of Indian mustard from India mostly contained dominant fertility restorer genes, while European varieties had a greater frequency of the recessive maintainer genes. None of these varieties, however, was capable of complete maintenance of male sterility. Heterosis for yield up to 56% over the national check was observed in field trials. The use of this cytoplasmic male sterile plant in hybrid mustard production will not be economical, until a complete maintainer for male sterility is identified.Key words: Brassica juncea, Indian mustard, male sterility, hybrids.

Transfer of a species cytoplasm specific (scs) gene from Triticum timopheevii to T. turgidum

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 238-243 ◽  
Author(s):  
S. S. Maan
Keyword(s):  
Common Wheat ◽  
Triticum Turgidum ◽  
Seed Viability ◽  
Male Parent ◽  
Alien Chromosome ◽  
Male Sterile ◽  
Male Sterile Plant ◽  
B Genome ◽  
Aegilops Longissima ◽  
Genome Donor

Initial attempts to substitute euploid nuclei of Triticum turgidum L. or T. aestivum L. into Aegilops longissima S. &L. cytoplasm failed because an alien chromosome remained fixed in the Triticum nucleus. The alien chromosome had gene(s) conditioning sporophytic sterility (also known as the gameticidal or Cuckoo effect). Subsequently, an exceptional 29-chromosome, male-sterile plant with spontaneously improved female fertility was used as a source of Ae. longissima cytoplasm, and a fully fertile alloplasmic common wheat 'Selkirk' line was developed. However, alloplasmic 'Selkirk' crossed with durum wheat as a recurrent male parent did not produce euploid plants. Instead, chromosome 1D or telocentric 1DL of 'Selkirk' was retained and male-sterile plants with 29 chromosomes were obtained. They set two seed types: a few that were plump and viable (PVi) and a large number that were shrivelled and inviable (SIv). The 1DL was deleted by crossing these plants to T. timopheevii, backcrossing the F1's to T. timopheevii, and repeatedly backcrossing the timopheevii-like plants to durum as the recurrent male parent. The resulting euploid durum plants with Ae. longissima cytoplasm were male sterile and set a 1:1 ratio of PVi and SIv seeds. Thus, a species cytoplasm specific (scs) gene of T. timopheevii was transferred to durum and caused male sterility and abortion of embryos lacking this gene. In conclusion, (i) the scs gene was expressed as a dominant sterility gene, restored seed viability, and partial compatibility between the durum nucleus and Ae. longissima cytoplasm and (ii) a scs gene on 1DL also caused dominant sterility in durum but not in alloplasmic common wheat. Hence, alien scs homoeoallele(s) conditioned sterility and seed abortion in alloplasmic durum but not in T. aestivum and T. timopheevii.Key words: interspecific nucleocytoplasmic genetics, sporophytically controlled sterility, B-genome donor, scs gene.

Agrobacterium-mediated transformation and generation of male sterile lines of Australian canola

2005 ◽  
Vol 56 (4) ◽  
pp. 353 ◽  
Author(s):  
Yan Zhang ◽  
Mohan B. Singh ◽  
Ines Swoboda ◽  
Prem L. Bhalla
Keyword(s):  
Transgenic Plants ◽  
Leaf Disc ◽  
Gus Expression ◽  
Male Sterile ◽  
Root Explants ◽  
Agrobacterium Mediated Transformation ◽  
Seedling Explants ◽  
Antisense Approach ◽  
Histochemical Tests ◽  
Transgenic Male

An efficient protocol for Agrobacterium-mediated transformation of Australian commercial canola cultivars using seedling explants is described. Seedling explants provide flexibility and reduction in labour and maintenance costs of explant sources. Five commercial genotypes of canola were successfully transformed using the developed protocol. A transformation efficiency of 67% was obtained for genotypes Oscar and RK7 from cotyledon explants, which was higher than the rate for the most commonly used cultivar Westar (33%). Comparison of different seedling explants showed that although transgenic plants could be regenerated from all explant types (cotyledons, hypocotyls, and roots) used, the number of plants regenerated per explant type varied among the cultivars. Cotyledons produced the maximum number of transgenic shoots (RK7, RI25, Oscar, and Westar cultivars), whereas root explants produced the lowest numbers of shoots. Therefore, cotyledons and hypocotyls can be considered as ideal explants for the Agrobacterium-mediated transformation of these Australian canola cultivars. Integration and expression of the introduced transgene were analysed by DNA gel blot, leaf disc test, and GUS expression assays. Analysis of progeny showed that the transgene was stably inherited. The possibility of producing male sterile lines using an antisense approach was also explored. For this, Bcp1, a gene shown to be vital for viable pollen development, was targetted. Pollen ablation and lack of seed set were observed in the transgenic plants. Histochemical tests showed an intact tapetum layer and well developed pollen in control plants, whereas degraded tapetum and ablated pollen were noted in the transgenic plants. These results indicate that it is possible to generate stable transgenic male-sterile lines of canola using this strategy.

scholarly journals CYTOPLASMIC STERILITY FACTORS IN VICIA FABA L

Genetics ◽  
1976 ◽  
Vol 82 (3) ◽  
pp. 443-449
Author(s):  
J R Edwardson ◽  
D A Bond ◽  
R G Christie
Keyword(s):  
Vicia Faba ◽  
Sterile Plant ◽  
Fertile Plant ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Vicia Faba L ◽  
Ultrathin Sections ◽  
Cytoplasmic Sterility

ABSTRACT Tissues of cytoplasmic male sterile, maintainer, restorer, and restored lines, and sterile plants which reverted to fertility in Vicia faba were examined in ultrathin sections. Cytoplasmic spherical bodies (CSB), ca. 70 nm in diameter, were observed in tissues of all sterile plants but not in tissues of maintainer, restorer or restored sterile plants. No CSB were observed in a reverted fertile branch of a tiller-sterile plant, nor in 5 of 6 reverted fertile plants. One reverted fertile plant contained CSB in ovules. It is proposed that the CSB are the sites of, or possibly, products of, sterility factors in Vicia faba.

scholarly journals Toward the Identification of Cytoplasmic Male Sterility in Leek: Evaluation of Organellar DNA Diversity among Cultivated Accessions of Allium ampeloprasum

1999 ◽  
Vol 124 (2) ◽  
pp. 163-165 ◽  
Author(s):  
Michael J. Havey ◽  
Daniela Lopes Leite
Keyword(s):  
Male Sterility ◽  
Chloroplast Genome ◽  
Male Sterile ◽  
Male Sterile Plant ◽  
Asexual Propagation ◽  
Allium Ampeloprasum ◽  
Cytoplasmic Diversity ◽  
Length Polymorphisms ◽  
Mitochondrial Dnas ◽  
Great Headed Garlic

Hybrid leek is more uniform and higher yielding than open-pollinated cultivars and is presently produced by asexual propagation of a genic male-sterile plant. A cheaper method to produce hybrid leek seed would be a system of cytoplasmic-genic male sterility (CMS). Restriction fragment length polymorphisms (RFLPs) in the organellar genomes have correlated with CMS in many crops. We undertook gel-blot analyses of the chloroplast and mitochondrial DNAs to assess cytoplasmic diversity among 62 accessions of the major cultivated forms of Allium ampeloprasum L. (leek, kurrat, and great-headed garlic). No polymorphisms were detected in the chloroplast genome of leek and kurrat. Three accessions of leek and one of kurrat possessed one or two of seven polymorphic mitochondrial probe-enzyme combinations. Great-headed garlic differed from leek and kurrat for six polymorphisms in the chloroplast genome and for many mitochondrial probe-enzyme combinations. Our analyses revealed few organellar polymorphisms among accessions of leek and kurrat, reducing the probability that selection of polymorphic cytoplasms will reveal CMS in leek.

Sign in / Sign up

Export Citation Format

Share Document