scholarly journals Inhibition of the p44/42 MAP kinase pathway protects hippocampal neurons in a cell-culture model of seizure activity

1998 ◽  
Vol 95 (20) ◽  
pp. 11975-11980 ◽  
Author(s):  
B. Murray ◽  
A. Alessandrini ◽  
A. J. Cole ◽  
A. G. Yee ◽  
E. J. Furshpan
Keyword(s):  
Cell Culture ◽  
Map Kinase ◽  
Hippocampal Neurons ◽  
Seizure Activity ◽  
Cell Culture Model ◽  
Culture Model ◽  
A Cell ◽  
Map Kinase Pathway ◽  
Kinase Pathway

Juglone Exerts Cytotoxic, Anti-proliferative and Anti-invasive Effects on Glioblastoma Multiforme in a Cell Culture Model

2016 ◽  
Vol 16 (9) ◽  
pp. 1190-1197 ◽  
Author(s):  
Dziugas Meskelevicius ◽  
Kastytis Sidlauskas ◽  
Ruta Bagdonaviciute ◽  
Julius Liobikas ◽  
Daiva Majiene
Keyword(s):  
Cell Culture ◽  
Glioblastoma Multiforme ◽  
Cell Culture Model ◽  
Culture Model ◽  
A Cell

scholarly journals Zinc, Zinc Transporters, and Cadmium Cytotoxicity in a Cell Culture Model of Human Urothelium

Toxics ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 94
Author(s):  
Soisungwan Satarug ◽  
Scott H. Garrett ◽  
Seema Somji ◽  
Mary Ann Sens ◽  
Donald A. Sens
Keyword(s):  
Dna Methylation ◽  
Cell Culture ◽  
Cell Viability ◽  
Zinc Transporters ◽  
Cell Culture Model ◽  
Induced Expression ◽  
Glutathione Biosynthesis ◽  
Culture Model ◽  
A Cell ◽  
Cd Exposure

We explored the potential role of zinc (Zn) and zinc transporters in protection against cytotoxicity of cadmium (Cd) in a cell culture model of human urothelium, named UROtsa. We used real-time qRT-PCR to quantify transcript levels of 19 Zn transporters of the Zrt-/Irt-like protein (ZIP) and ZnT gene families that were expressed in UROtsa cells and were altered by Cd exposure. Cd as low as 0.1 µM induced expression of ZnT1, known to mediate efflux of Zn and Cd. Loss of cell viability by 57% was seen 24 h after exposure to 2.5 µM Cd. Exposure to 2.5 µM Cd together with 10–50 µM Zn prevented loss of cell viability by 66%. Pretreatment of the UROtsa cells with an inhibitor of glutathione biosynthesis (buthionine sulfoximine) diminished ZnT1 induction by Cd with a resultant increase in sensitivity to Cd cytotoxicity. Conversely, pretreatment of UROtsa cells with an inhibitor of DNA methylation, 5-aza-2’-deoxycytidine (aza-dC) did not change the extent of ZnT1 induction by Cd. The induced expression of ZnT1 that remained impervious in cells treated with aza-dC coincided with resistance to Cd cytotoxicity. Therefore, expression of ZnT1 efflux transporter and Cd toxicity in UROtsa cells could be modulated, in part, by DNA methylation and glutathione biosynthesis. Induced expression of ZnT1 may be a viable mechanistic approach to mitigating cytotoxicity of Cd.

Bai Hu Tang, Si Ni Tang, and Xue Bi Tang amplify pro-inflammatory activities and reduce apoptosis in endothelial cells in a cell culture model of sepsis

2018 ◽  
Vol 225 ◽  
pp. 309-318 ◽  
Author(s):  
Dagmar Brislinger ◽  
Christine Daxböck ◽  
Eva Roßmanith ◽  
Manuela Stückler ◽  
Ingrid Lang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Cell Culture Model ◽  
Culture Model ◽  
A Cell

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

10.3791/61388 ◽  
2020 ◽  
Author(s):  
Genilso Gava-Junior ◽  
Cláudio Roque ◽  
Julieta Mendes-Oliveira ◽  
Ana C Bernardino ◽  
Inês Serrenho ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Culture Model ◽  
Culture Model ◽  
A Cell
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