scholarly journals Rational design of a scytalone dehydratase-like enzyme using a structurally homologous protein scaffold

1999 ◽  
Vol 96 (7) ◽  
pp. 3568-3571 ◽  
Author(s):  
A. E. Nixon ◽  
S. M. Firestine ◽  
F. G. Salinas ◽  
S. J. Benkovic
Keyword(s):  
Rational Design ◽  
Protein Scaffold ◽  
Homologous Protein ◽  
Scytalone Dehydratase

Rational design of artificial dye-decolorizing peroxidases using myoglobin by engineering Tyr/Trp in the heme center

2017 ◽  
Vol 46 (34) ◽  
pp. 11230-11238 ◽  
Author(s):  
Le-Le Li ◽  
Hong Yuan ◽  
Fei Liao ◽  
Bo He ◽  
Shu-Qin Gao ◽  
...  
Keyword(s):  
Rational Design ◽  
Catalytic Performance ◽  
Protein Scaffold

Artificial dye-decolorizing peroxidases (DyPs) have been rationally designed using myoglobin (Mb) as a protein scaffold by engineering Tyr/Trp in the heme center, such as F43Y/F138 W Mb, which exhibited catalytic performance comparable to some native DyPs.

scholarly journals Rational Design of Dual Active Sites in a Single Protein Scaffold: A Case Study of Heme Protein in Myoglobin

ChemistryOpen ◽  
2016 ◽  
Vol 5 (3) ◽  
pp. 192-196 ◽  
Author(s):  
Xiao-Gang Shu ◽  
Ji-Hu Su ◽  
Ke-Jie Du ◽  
Yong You ◽  
Shu-Qin Gao ◽  
...  
Keyword(s):  
Active Sites ◽  
Rational Design ◽  
Heme Protein ◽  
Protein Scaffold ◽  
Single Protein

scholarly journals Rational Design of a Mononuclear Metal Site into the Archaeal Rieske-type Protein Scaffold

2005 ◽  
Vol 280 (10) ◽  
pp. 9129-9134 ◽  
Author(s):  
Toshio Iwasaki ◽  
Asako Kounosu ◽  
Ye Tao ◽  
Zhongrui Li ◽  
Jacob E. Shokes ◽  
...  
Keyword(s):  
Rational Design ◽  
Protein Scaffold ◽  
Metal Site ◽  
Type Protein

Molecular insights on the dynamic stability of peptide nucleic acid functionalized carbon and boron nitride nanotubes

2021 ◽  
Vol 23 (1) ◽  
pp. 219-228
Author(s):  
Nabanita Saikia ◽  
Mohamed Taha ◽  
Ravindra Pandey
Keyword(s):  
Nucleic Acid ◽  
Boron Nitride ◽  
Nucleic Acids ◽  
Dynamic Stability ◽  
Peptide Nucleic Acid ◽  
Rational Design ◽  
Peptide Nucleic Acids ◽  
Boron Nitride Nanotubes ◽  
Molecular Hybrids ◽  
Single Wall Nanotubes

The rational design of self-assembled nanobio-molecular hybrids of peptide nucleic acids with single-wall nanotubes rely on understanding how biomolecules recognize and mediate intermolecular interactions with the nanomaterial's surface.

Optimizing electron density of nickel sulfide electrocatalysts through sulfur vacancy engineering for alkaline hydrogen evolution

2020 ◽  
Vol 8 (35) ◽  
pp. 18207-18214
Author(s):  
Dongbo Jia ◽  
Lili Han ◽  
Ying Li ◽  
Wenjun He ◽  
Caichi Liu ◽  
...  
Keyword(s):  
Hydrogen Evolution ◽  
Electron Density ◽  
Rational Design ◽  
Nickel Sulfide ◽  
Catalytic Performance ◽  
Sulfide Catalysts ◽  
Sulfur Vacancy

A novel, rational design for porous S-vacancy nickel sulfide catalysts with remarkable catalytic performance for alkaline HER.

Rational design of annotine analogues with increased inhibitory activity towards acetylcholinesterase

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
ES Halldorsdottir ◽  
S Oddsson ◽  
AM Einarsdottir ◽  
B Eiriksdottir ◽  
NM Kowal ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Rational Design

Anticoagulant Activity of Hirulog™, a Direct Thrombin Inhibitor, in Humans

1993 ◽  
Vol 69 (02) ◽  
pp. 157-163 ◽  
Author(s):  
Irving Fox ◽  
Adrian Dawson ◽  
Peter Loynds ◽  
Jane Eisner ◽  
Kathleen Findlen ◽  
...  
Keyword(s):  
Rational Design ◽  
Therapeutic Potential ◽  
Anticoagulant Activity ◽  
Subcutaneous Administration ◽  
Direct Thrombin Inhibitor ◽  
Controlled Study ◽  
Thrombin Inhibitor ◽  
Thrombin Time ◽  
Thrombotic Disease ◽  
Dose Dependent

SummaryHirulog™ (BG8967) is a direct thrombin inhibitor built by rational design using the protein hirudin as a model (Maraganore et al. [1990]; Biochemistry 29: 7095–101). In order to evaluate the therapeutic potential for hirulog in the management of thrombotic disease, the tolerability and anticoagulant activity of the agent were examined in a study of human volunteers.In a randomized, placebo-controlled study (n = 54), the intravenous infusion of hirulog over 15 min showed a rapid, dose-dependent prolongation of activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). There was a corresponding dose-dependent increase in plasma hirulog levels. The peptide was rapidly cleared with a half-life of 36 min and a total body clearance rate for the peptide of 0.43 1 kg−1 h−1. Similar activity was observed following subcutaneous injection but with sustained pharmacodynamic and pharmacokinetic behavior. There was a significant correlation between pharmacokinetic and pharmacodynamic variables for both intravenous (r = 0.8, p <0.001) and subcutaneous administration (r = 0.7, p = 0.002).To evaluate the possible interactions of aspirin on the tolerability and anticoagulant activity of intravenous hirulog, a cross-over design was employed in eight subjects. Aspirin administration did not modify the peptide’s activity. At the administered dose of 0.6 mg kg−1 h−1 for 2 h, hirulog infusion prolonged APTT from 230 to 260% baseline. The infusion of hirulog in subjects who had received aspirin was not associated with any significant changes in the template bleeding time.The final phase of the study examined the activity and tolerability of hirulog in ten subjects during prolonged intravenous infusions for up to 24 h. The peptide (0.3 mg kg−1 h−1) exhibited sustained anticoagulant activity with no evidence for a cumulative effect. During hirulog infusion, APTT was prolonged from 210 to 250% baseline.In all phases of the study, hirulog administration was generally well-tolerated.Our observations show that hirulog is an active antithrombin agent with excellent tolerability in humans. As a direct thrombin inhibitor, hirulog provides a novel approach for the management of thrombotic disease.

Development of a rational design methodology for precast concrete slender spandrel beams: Part 1, experimental results

PCI Journal ◽  
2011 ◽  
Vol 56 (2) ◽  
pp. 88-112 ◽  
Author(s):  
Gregory Lucier ◽  
Catrina Walter ◽  
Sami Rizkalla ◽  
Paul Zia ◽  
Gary Klein
Keyword(s):  
Design Methodology ◽  
Rational Design ◽  
Precast Concrete ◽  
Experimental Results
Sign in / Sign up

Export Citation Format

Share Document